4 mg/ml in water
add HCl to dissolve if necessary
filtersterilize
tyrosine: 2 mg/ml
add NaOH to dissolve
filtersterilize
dropout mix: all amino acids
except one at 2 mg/ml each in water - do not add tyrosine
dissolve amino acids one
by one
filtersterilize
DNA loading buffer
40% (v/v) glycerol
0.25% xylene cyanol
0.25% bromophenolblue
2% v/v 50x TBE
DEPC-treated water
add DEPC (diethylpyrocarbonate)
to 0.1%
stir for at least 30 min.
autoclave for 30 min.
keep at room temperature
with loose cap untill cool
20xSSC can also be treated with DEPC
solutions containing amines
(including Tris!) can NOT be treated with DEPC
for RNA work, use HEPES
as buffer when possible, as this can be DEPCed
EDTA
0.5 M = 186.1 g/l
needs NaOH to dissolve
set pH to 8.0 with NaOH
ethidium bromide
10.000 x = 5 mg/ml in water
carcinogenic !
buy dissolved ethidium bromide
instead of playing with the powder yourself if possible
remember: machines don't
get cancer!
20x SSC
3 M NaCl, 0.3 M sodium citrate
per liter: 880 g NaCl, 440 g sodium citrate
may be autoclaved or treated
with DEPC
TE
10 mM Tris , pH 7 or 8 (or
anything in between) with HCl
1 mM EDTA
can be prepared as 50x stock
solution
TES
10 mM Tris , pH 7 or 8 (or
anything in between) with HCl
1 mM EDTA
0.1% SDS
can be prepared as 50x stock
solution
Tris
1M = 121.1 g/l
set pH with HCl
X-Gal
1000x stock = 35 mg/ml in DMF (dimethylformamide)
store frozen & keep dark