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culture media for yeast and E. coli
link to antibiotics stock solutions

link to general stock solutions

minimal media for E. coli @ the bottom of the page

SOB

(E. coli)

2% bactotryptone
0.5% yeast extract
10 mM NaCl
2.5 mM KCl
10 mM MgCl2
10 mM MgSO4

autoclave

SOC

(E.coli)

SOB + 20 mM glucose

autoclave for 10 min.

YNB

(yeast)

YNB = yeast nitrogen base

10x YNB stock (100 ml): 6.7 g YNB w/o amino acids + carbon/energy source

if you don't use amino acids as selection marker, use YNB+amino acids for faster growth

10x YNB + glucose (100 ml): 6.7 g YNB, 20 g glucose

filtersterilize or autoclave for 10 min.

YPD

(yeast and E. coli)

1% yeast extract
2% bactopeptone
2% glucose

(add 2% agar for plates)

autoclave for 10 min.

2YT

(E. coli and yeast)

1.6% bactotryptone
1% yeast extract
0.5% NaCl

(add 1.5% agar for plates)

autoclave

minimal media for E. coli

MOPS buffered minimal medium for E. coli and S. typhimurium (adapted from Neidhart et al.(1974) J Bacteriol 119, 736-47)

for 1 liter:

100 ml 10x MS
1 ml 1.32 M K2HPO4
40 ml 1M MOPS pH 7.4
10 ml 50% glucose
2.5 ml 4 mg/ml thiamine
amino acids (at 4 mg/ml = 100x), antibiotics etc. as required (for MC1000-derived strains: valine, leucine, isoleucine, thiamine)

10x MS and K2HPO4 can be autoclaved, the rest should be filtersterilized

for 1 liter 10x MS:
40 ml 1 M Tricine (freshly made), pH 7.4 with 50% KOH
50 ml 1.9 M NH4Cl
10 ml 0.276 M K2SO4
10 ml 10 mM FeSO4 (freshly made)
10 ul (!) 0.5 M CaCl2
5.3 ml 1 M MgCl2
100 ml 5 M NaCl
100 ul (!) 10.000x micronutrients

tricine and FeSO4 should be filtersterilized, the rest can be autoclaved

for 10.000x micronutrients:
0.3 mM (NH4)6(Mo7)24
40 mM H3BO3
3 mM CoCl2
1 mM CuSO4
8 mM MnCl2
1 mM ZnSO4

a variation on this theme to grow E. coli at different growth rates in media of equal osmolarity (Peter Huls, Univ Amsterdam):
osmolarity = 300 mosM
 
for 1 liter: glucose (normal growth) proline (slow growth) glu + AA (turbo growth)
10x MS 100 ml 100 ml 100 ml
1 M MOPS pH 7.6 40 ml 40 ml 40 ml
1.32 M K2HPO4 1 ml 1 ml 1 ml
4 mg/ml thiamine 2.5 ml 2.5 ml 2.5 ml
antibiotics as required as required as required
5 M NaCl 21.6 ml 24 ml 21.6 ml
50% glucose 10 ml - 10 ml
40 mg/ml proline - 20 ml -
amino acid mix* - - 20 ml
2 mg/ml tyrosine - - 20 ml
amino acids** as required (leu, ile, and val for MC1000 and derivatives)

*all amino acids except tyrosine, 2 mg/ml each
** 4 mg/ml = 100x stock
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