Mascot Search Results

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MS data file    : cw0349_q2.pkl
Database        : spombe  (5049 sequences; 2391832 residues)
Timestamp       : 10 Oct 2006 at 11:05:58 GMT
Significant hits: mSPCC1322.10  |predicted|: Predicted GPI protein

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 30 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    mSPCC1322.10                      Mass: 23944    Score: 35     Queries matched: 1
 |predicted|: Predicted GPI protein
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   423.75   845.49   845.46   0.03 0  35  0.018 1  -.VSVTSPTR.D

2.    SPBC32H8.13c                      Score: 13     Queries matched: 1
 |mok12||alpha-1,3-glucan synthase|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   847.43   -1.94 0  13  2.6 2  K.TVSTPSEK.L

3.    SPCC5E4.04                        Score: 8      Queries matched: 1
 |cut1||separin|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   845.43   0.06 0  11  4.7 3  K.VSWTPEK.F

4.    SPBP22H7.05c                      Score: 7      Queries matched: 1
 |||TAT-binding protein homolog|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   845.40   0.08 0  7  13 4  K.WVGEAER.Q

 
      Proteins matching the same set of peptides:
      SPAC31G5.19                       Score: 7      Queries matched: 1
5.    SPBC31E1.01c                      Mass: 185106   Score: 2      Queries matched: 1
 ||SPBC660.18c|zinc finger protein |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   453.77   905.52   906.42   -0.89 0  2  28 1  R.GSNASASASR.A

6.    SPAC1399.05c                      Mass: 61203    Score: 2      Queries matched: 1
 |||zinc finger protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   845.44   0.05 1  2  35 6  M.GEVERTR.Q

7.    SPBP8B7.29                        Score: 1      Queries matched: 1
 |||para-aminobenzoate synthase |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   453.77   905.52   904.46   1.06 0  1  35 2  K.ATNTTEIR.I

8.    SPAC25B8.06c                      Score: 1      Queries matched: 1
 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   453.77   905.52   906.47   -0.94 1  1  38 3  K.ENRGLYR.L

9.    SPAC637.06                        Mass: 41386    Score: 1      Queries matched: 1
 |||galactosyltransferase|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   453.77   905.52   906.43   -0.91 1  1  41 4  R.RSDWTSR.L

10.   SPAC328.03                        Score: 0      Queries matched: 1
 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-forming]|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   843.49   1.99 1  0  52 9  R.IASIERR.L

11.   SPBC12C2.05c                      Score: 0      Queries matched: 1
 |||DAG domain|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   847.44   -1.95 1  2  36 7  K.ERSSIEK.E

12.   SPBC29A10.04                      Score: 0      Queries matched: 1
 |psm1|smc1|mitotic cohesin complex |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   845.47   0.01 1  1  50 8  R.VSDTRLR.L

13.   SPBC30D10.10c                     Score: 0      Queries matched: 1
 |tor1||phosphatidylinositol kinase|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   845.50   -0.01 1  4  23 5  R.VSNKLTGK.D

14.   SPCC584.04                        Score: 0      Queries matched: 1
 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   844.48   1.01 1  0  52 9  K.GKTVEVGR.A

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 2 Da
Fragment Mass Tolerance: ± 0.8 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 2

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 28 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 34.9    0.03    1  mSPCC1322.10  -.VSVTSPTR.D
 13.4   -1.94    2  SPBC32H8.13c  K.TVSTPSEK.L
 10.8    0.06    3  SPCC5E4.04    K.VSWTPEK.F
  6.5    0.08    4  SPBP22H7.05c  K.WVGEAER.Q
  3.9   -0.01   13  SPBC30D10.10c R.VSNKLTGK.D
  2.0    0.05    6  SPAC1399.05c  M.GEVERTR.Q
  1.9   -1.95   11  SPBC12C2.05c  K.ERSSIEK.E
  0.5    0.01   12  SPBC29A10.04  R.VSDTRLR.L
  0.4    1.01   14  SPCC584.04    K.GKTVEVGR.A
  0.4    1.99   10  SPAC328.03    R.IASIERR.L
Top scoring peptide matches to query 2
Score greater than 14 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  2.1   -0.89    5  SPBC31E1.01c R.GSNASASASR.A
  1.2    1.06    7  SPBP8B7.29   K.ATNTTEIR.I
  0.9   -0.94    8  SPAC25B8.06c K.ENRGLYR.L
  0.5   -0.91    9  SPAC637.06   R.RSDWTSR.L