Mascot Search Results

User            : 
Email           : 
Search title    : 
MS data file    : cw0349_q1.pkl
Database        : spombe  (5049 sequences; 2391832 residues)
Timestamp       : 10 Oct 2006 at 09:39:09 GMT
Significant hits: mSPCC1322.10  |predicted|: Predicted GPI protein
                  TRBOTR        trypsin (EC3.4.21.4 ) precursor-bovine

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 31 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

  Help
  Significance threshold p< Max. number of hits  
  Standard scoring  MudPIT scoring  Ions score cut-off Show sub-sets
  Show pop-ups  Suppress pop-ups  Sort unassigned Require bold red

        Error tolerant   

1.    mSPCC1322.10                      Mass: 23944    Score: 45     Queries matched: 1
 |predicted|: Predicted GPI protein
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   423.75   845.49   845.46   0.03 0  45  0.0024 1  -.VSVTSPTR.D

2.    TRBOTR                            Mass: 24662    Score: 34     Queries matched: 1
 trypsin (EC3.4.21.4 ) precursor-bovine
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   453.77   905.52   905.50   0.02 0  34  0.024 1  K.NKPGVYTK.V

 
      Proteins matching the same set of peptides:
      TRY2_CANFA                        Mass: 27090    Score: 34     Queries matched: 1
 Trypsin, anionic precursor (EC 3.4.21.4).
3.    SPAC25B8.06c                      Mass: 51692    Score: 16     Queries matched: 1
 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   453.77   905.52   906.47   -0.95 1  16  1.6 2  K.ENRGLYR.L

4.    SPCC5E4.04                        Score: 14     Queries matched: 1
 |cut1||separin|Schizosaccharomyces pombe|chr 3|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   845.43   0.06 0  17  1.5 2  K.VSWTPEK.F

5.    SPBC32H8.13c                      Score: 13     Queries matched: 1
 |mok12||alpha-1,3-glucan synthase|Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   847.43   -1.94 0  13  3.5 3  K.TVSTPSEK.L

6.    SPACUNK4.13c                      Score: 10     Queries matched: 1
 |||GTP binding|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   453.77   905.52   906.50   -0.98 2  10  6.2 3  K.SVGKCKTK.G

7.    SPBC13G1.06c                      Mass: 11996    Score: 8      Queries matched: 1
 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   453.77   905.52   906.52   -1.00 2  8  8 4  R.DKFKELK.V

8.    SPBC3E7.05c                       Score: 7      Queries matched: 1
 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   453.77   905.52   904.50   1.02 0  7  12 5  R.LQQELFK.L

9.    SPBP22H7.05c                      Score: 5      Queries matched: 1
 |||TAT-binding protein homolog|Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   845.40   0.09 0  5  24 5  K.WVGEAER.Q

 
      Proteins matching the same set of peptides:
      SPAC31G5.19                       Score: 5      Queries matched: 1
10.   SPAC1399.05c                      Score: 3      Queries matched: 1
 |||zinc finger protein|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   845.44   0.05 1  3  34 6  M.GEVERTR.Q

11.   SPBC1861.02                       Score: 2      Queries matched: 1
 |abp2||ARS binding protein |Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   453.77   905.52   904.51   1.01 0  2  33 7  K.IQQYVVR.L

12.   SPBC83.01                         Score: 2      Queries matched: 1
 |ucp8||actin cortical patch component |Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   453.77   905.52   906.52   -1.00 0  2  33 8  K.AGDPPILPK.Q

13.   SPCC1235.05c                      Score: 2      Queries matched: 1
 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr 3|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   453.77   905.52   905.46   0.05 1  2  35 10  K.ELRETMK.G

14.   SPAC1952.02                       Score: 2      Queries matched: 1
 |||G-patch domain|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   845.53   -0.04 2  2  49 8  K.VSGTKKVK.A

15.   SPBC1105.08                       Score: 1      Queries matched: 1
 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   847.44   -1.95 0  1  50 10  K.LMQGMLR.K

16.   SPBC30D10.10c                     Score: 1      Queries matched: 1
 |tor1||phosphatidylinositol kinase|Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   845.50   -0.01 1  6  17 4  R.VSNKLTGK.D

17.   SPBC21D10.12                      Score: 0      Queries matched: 1
 |hob1||amphiphysin homolog|Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   453.77   905.52   906.48   -0.96 0  3  30 6  K.FNVGEITK.D

18.   SPAC26A3.09c                      Score: 0      Queries matched: 1
 |rga2||GTPase activating protein|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   845.48   0.01 1  2  46 7  M.VKMDVVR.T

19.   SPBC29A10.04                      Score: 0      Queries matched: 1
 |psm1|smc1|mitotic cohesin complex |Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.75   845.49   845.47   0.02 1  2  49 8  R.VSDTRLR.L

20.   SPBC725.06c                       Score: 0      Queries matched: 1
 |||serine/threonine protein kinase |Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   453.77   905.52   906.49   -0.97 1  2  33 9  K.ENRTFIK.K

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 2 Da
Fragment Mass Tolerance: ± 0.8 Da
Max Missed Cleavages   : 2
Instrument type        : ESI-QUAD-TOF
Number of queries      : 2

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 44.6    0.03    1  mSPCC1322.10  -.VSVTSPTR.D
 16.7    0.06    4  SPCC5E4.04    K.VSWTPEK.F
 13.0   -1.94    5  SPBC32H8.13c  K.TVSTPSEK.L
  6.2   -0.01   16  SPBC30D10.10c R.VSNKLTGK.D
  4.6    0.09    9  SPBP22H7.05c  K.WVGEAER.Q
  3.1    0.05   10  SPAC1399.05c  M.GEVERTR.Q
  1.8    0.01   18  SPAC26A3.09c  M.VKMDVVR.T
  1.5    0.02   19  SPBC29A10.04  R.VSDTRLR.L
  1.5   -0.04   14  SPAC1952.02   K.VSGTKKVK.A
  1.4   -1.95   15  SPBC1105.08   K.LMQGMLR.K
Top scoring peptide matches to query 2
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 33.6    0.02    2  TRBOTR       K.NKPGVYTK.V
 15.6   -0.95    3  SPAC25B8.06c K.ENRGLYR.L
  9.6   -0.98    6  SPACUNK4.13c K.SVGKCKTK.G
  8.4   -1.00    7  SPBC13G1.06c R.DKFKELK.V
  6.8    1.02    8  SPBC3E7.05c  R.LQQELFK.L
  2.8   -0.96   17  SPBC21D10.12 K.FNVGEITK.D
  2.4    1.01   11  SPBC1861.02  K.IQQYVVR.L
  2.3   -1.00   12  SPBC83.01    K.AGDPPILPK.Q
  2.3   -0.97   20  SPBC725.06c  K.ENRTFIK.K
  2.0    0.05   13  SPCC1235.05c K.ELRETMK.G