Mascot Search Results

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MS data file    : cw0347_q1.pkl
Database        : spombe  (5049 sequences; 2391832 residues)
Timestamp       : 29 Sep 2006 at 11:11:38 GMT
Significant hits: TRBOTR        trypsin (EC3.4.21.4 ) precursor-bovine
                  mSPCC1322.10  |predicted|: Predicted GPI protein
                  SPCC1020.01c  |pma2|SPCC1393.01|P-type proton ATPase |Schizosaccharomyces pombe|chr 3|||Manual

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 28 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    TRBOTR                            Mass: 24662    Score: 248    Queries matched: 3
 trypsin (EC3.4.21.4 ) precursor-bovine
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   577.31   1152.61   1152.57   0.05 0  43  0.002 1  K.SSGTSYPDVLK.C
5   1082.08   2162.14   2162.05   0.09 0  139  4e-013 1  R.LGEDNINVVEGNEQFISASK.S
6   758.41   2272.20   2272.15   0.05 0  66  8.7e-006 1  K.SIVHPSYNSNTLNNDIMLIK.L

2.    mSPCC1322.10                      Mass: 23944    Score: 41     Queries matched: 1
 |predicted|: Predicted GPI protein
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   423.76   845.51   845.46   0.05 0  41  0.0052 1  -.VSVTSPTR.D

3.    SPCC1020.01c                      Mass: 110743   Score: 29     Queries matched: 1
 |pma2|SPCC1393.01|P-type proton ATPase |Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   993.09   1984.17   1984.12   0.04 0  29  0.037 1  R.SAADIVFLAPGLSAIIDALK.T

 
      Proteins matching the same set of peptides:
      SPAC1071.10c                      Mass: 100391   Score: 29     Queries matched: 1
 |pma1||P-type proton ATPase |Schizosaccharomyces pombe|chr 1|||Manual
4.    SPBC32H8.13c                      Score: 14     Queries matched: 1
 |mok12||alpha-1,3-glucan synthase|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.76   845.51   847.43   -1.92 0  14  2.9 2  K.TVSTPSEK.L

5.    SPCC5E4.04                        Score: 10     Queries matched: 1
 |cut1||separin|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.76   845.51   845.43   0.08 0  13  3.6 3  K.VSWTPEK.F

6.    SPBC887.14c                       Score: 8      Queries matched: 1
 |pfh1|pif1|DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   577.31   1152.61   1151.60   1.02 2  8  6.7 2  K.SMLSSEKRSK.N

7.    SPBC25H2.11c                      Score: 7      Queries matched: 1
 |||bromodomain protein|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   577.31   1152.61   1152.66   -0.05 1  7  7.9 3  R.LPPNGKITTGR.K

8.    SPCC1672.11c                      Score: 5      Queries matched: 1
 |||P-type ATPase |Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.76   845.51   847.48   -1.97 0  5  20 5  K.STTATVIR.E

9.    SPBC3F6.05                        Score: 5      Queries matched: 1
 |rga1||GTPase activating protein|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   577.31   1152.61   1152.50   0.11 1  5  12 4  R.RMDAFDENR.A

10.   SPBC1289.16c                      Mass: 90755    Score: 5      Queries matched: 1
 ||SPBC8E4.06|copper amine oxidase |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   681.87   1361.72   1362.72   -1.00 0  5  15 1  R.ISLTEMAVPFGAK.G

11.   SPBC115.02c                       Score: 4      Queries matched: 1
 |||ATPase |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   577.31   1152.61   1152.58   0.04 0  4  15 5  K.SAADYLSLASR.Y

12.   SPAP27G11.09c                     Score: 4      Queries matched: 1
 |||GTP cyclohydrolase |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   577.31   1152.61   1152.59   0.02 0  4  16 6  R.GAYVGTLYPGR.K

13.   SPBC106.06                        Score: 4      Queries matched: 1
 |cct4||chaperonin-containing T-complex|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.76   845.51   847.46   -1.95 0  7  15 4  K.TVSILCR.G

14.   SPBC16A3.18                       Score: 4      Queries matched: 1
 |||hypothetical protein|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   577.31   1152.61   1152.63   -0.01 0  4  17 7  K.LQPIGSPQASR.K

15.   SPBC19F5.04                       Score: 4      Queries matched: 1
 |||aspartate kinase |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   758.41   2272.20   2272.26   -0.06 2  4  13 2  K.GATAVTIKDTIMVINIQSNRK.I

16.   SPBC1709.09                       Score: 4      Queries matched: 1
 |||involved in mitochondrial protein synthesis|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   758.41   2272.20   2271.07   1.12 2  4  14 3  K.DVKHDHDPEFANSFNKMLK.S

17.   SPBC18H10.05                      Score: 3      Queries matched: 1
 |||WD repeat protein|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   758.41   2272.20   2272.22   -0.02 2  3  18 5  K.VCSERLTSTIIAPARTAVEAK.E

18.   SPAC2F3.11                        Score: 3      Queries matched: 1
 |||exopolyphosphatase |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   577.31   1152.61   1152.52   0.10 0  3  24 9  K.DEGGSLHADPR.I

19.   SPBP22H7.05c                      Score: 3      Queries matched: 1
 |||TAT-binding protein homolog|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.76   845.51   845.40   0.11 0  3  38 9  K.WVGEAER.Q

 
      Proteins matching the same set of peptides:
      SPAC31G5.19                       Score: 3      Queries matched: 1
20.   SPAC1399.05c                      Score: 3      Queries matched: 1
 |||zinc finger protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   423.76   845.51   845.44   0.07 1  3  38 9  M.GEVERTR.Q

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   1290.32   3867.93        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 2 Da
Fragment Mass Tolerance: ± 0.8 Da
Max Missed Cleavages   : 2
Instrument type        : ESI-QUAD-TOF
Number of queries      : 7

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 29 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 41.3    0.05    2  mSPCC1322.10 -.VSVTSPTR.D
 13.8   -1.92    4  SPBC32H8.13c K.TVSTPSEK.L
 12.8    0.08    5  SPCC5E4.04   K.VSWTPEK.F
  6.7   -1.95   13  SPBC106.06   K.TVSILCR.G
  5.4   -1.97    8  SPCC1672.11c K.STTATVIR.E
  5.0    0.04                    R.VSDTRLR.L
  4.0    1.03                    K.GKTVEVGR.A
  4.0    0.03                    M.VKMDVVR.T
  2.5    0.07   20  SPAC1399.05c M.GEVERTR.Q
  2.5    0.11   19  SPBP22H7.05c K.WVGEAER.Q
Top scoring peptide matches to query 2
Score greater than 23 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 43.3    0.05    1  TRBOTR        K.SSGTSYPDVLK.C
  8.1    1.02    6  SPBC887.14c   K.SMLSSEKRSK.N
  7.4   -0.05    7  SPBC25H2.11c  R.LPPNGKITTGR.K
  5.4    0.11    9  SPBC3F6.05    R.RMDAFDENR.A
  4.5    0.04   11  SPBC115.02c   K.SAADYLSLASR.Y
  4.4    0.02   12  SPAP27G11.09c R.GAYVGTLYPGR.K
  3.9   -0.01   14  SPBC16A3.18   K.LQPIGSPQASR.K
  3.4    0.07                     K.DSMETMKRR.M
  2.6    0.10   18  SPAC2F3.11    K.DEGGSLHADPR.I
  2.4    0.98                     K.KSWKEYAIK.V
Top scoring peptide matches to query 3
Score greater than 17 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  5.0   -1.00   10  SPBC1289.16c R.ISLTEMAVPFGAK.G
  4.2    0.02                    K.QSFSITTSLNHK.L
  2.6    0.08                    K.KTSPQSSEDAANK.E
  0.4   -0.94                    R.KSAQLENETDTK.S
Top scoring peptide matches to query 4
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 29.3    0.04    3  SPCC1020.01c R.SAADIVFLAPGLSAIIDALK.T
Top scoring peptide matches to query 5
Score greater than 22 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
139.3    0.09    1  TRBOTR R.LGEDNINVVEGNEQFISASK.S
  0.7    0.06              R.IQPNEDVQSVVVHSNPSWK.N
Top scoring peptide matches to query 6
Score greater than 20 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 65.7    0.05    1  TRBOTR       K.SIVHPSYNSNTLNNDIMLIK.L
  3.8   -0.06   15  SPBC19F5.04  K.GATAVTIKDTIMVINIQSNRK.I
  3.7    1.12   16  SPBC1709.09  K.DVKHDHDPEFANSFNKMLK.S
  3.0   -1.07                    K.QKVSVPKGAYLLGVADETGTLK.G
  2.6   -0.02   17  SPBC18H10.05 K.VCSERLTSTIIAPARTAVEAK.E
  1.8    0.04                    R.IADYEDKKDGTSFPVSFLLK.S
  1.7    0.05                    K.SLGNVIDPMDIINGVTLENMK.K
  1.1   -1.01                    K.HSEFISYPIQLVVTREVEK.E
  0.6   -1.04                    K.KPEPYKGKGIYVDGEKPQLK.A
  0.5    0.02                    K.KLGADFTVDYHDPNVVEIIK.K
Top scoring peptide matches to query 7
Score greater than 23 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide