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MS data file    : cw0342_q4.pkl

Database        : spombe  (5049 sequences; 2391832 residues)

Timestamp       : 27 Oct 2006 at 16:00:52 GMT

Significant hits: SPAC13G6.10c  |||serine-rich protein|Schizosaccharomyces pombe|chr 1|||Manual

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 26 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    SPAC13G6.10c                      Mass: 54406    Score: 71     Queries matched: 1
 |||serine-rich protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   1033.91   2065.81   2066.01   -0.20 0  71  2.1e-006 1  R.GLAWIPGTDLGYSDNFVNK.G

 
      Proteins matching the same set of peptides:
      mSPAC13G6.10c                     Mass: 52568    Score: 71     Queries matched: 1
 |predicted| probably mild-alkali sensitive

2.    SPCC285.14                        Mass: 134045   Score: 9      Queries matched: 1
 |||TRAPP |Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   1041.92   2081.82   2082.07   -0.25 0  9  2.9 1  K.LFDSSIFSDYLLFFLQK.E

3.    SPBP23A10.08                      Score: 6      Queries matched: 1
 |alp5||actin-like protein|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   1033.91   2065.81   2066.02   -0.21 1  7  5 2  K.SKNPVEIGQPANCELRPR.D + 2 Deamidation (NQ)

4.    SPAC664.14                        Mass: 56161    Score: 4      Queries matched: 1
 |||Amt ammonium transporter|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   787.33   786.33   786.42   -0.09 1  4  10 1  K.LKSHMR.I + Oxidation (M)

5.    SPBC16H5.02                       Score: 3      Queries matched: 1
 |pfk1||6-phosphofructokinase |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   1033.91   2065.81   2066.02   -0.21 1  3  12 8  R.GWLAEGGTLIGTARCMAFR.E

6.    SPAC17H9.01                       Score: 2      Queries matched: 1
 |cid16||nucleotidyltransferase |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   787.33   786.33   786.46   -0.13 1  2  14 2  R.KISTPNK.L

7.    SPCC63.03                         Mass: 72414    Score: 2      Queries matched: 1
 |||DNAJ domain protein|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   1033.91   2065.81   2066.03   -0.23 1  4  11 4  R.LGQKISLPIMWCSVFDR.S + Deamidation (NQ); Oxidation (M)

8.    SPAC16E8.04c                      Score: 2      Queries matched: 1
 |||chorismate mutase |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   1033.91   2065.81   2066.07   -0.26 1  3  12 6  R.SALIRQEDTIIFNFLER.A + 2 Deamidation (NQ)

9.    SPAC3H8.09c                       Mass: 82778    Score: 1      Queries matched: 1
 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   970.88   1939.75   1939.80   -0.05 0  1  22 1  K.ANYGFVQFFTNEDCAR.A + 2 Deamidation (NQ)

10.   SPBC56F2.04                       Score: 1      Queries matched: 1
 |||snoRNA binding|Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   1033.91   2065.81   2066.05   -0.24 1  3  12 7  K.QLNMQRYDSFIKPSLPK.F + 2 Deamidation (NQ)

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   350.95   349.95        
2   437.02   436.02        
3   487.12   486.11        
4   649.15   648.14        
5   762.37   761.36        

Search Parameters

Type of search         : MS/MS Ion Search

Enzyme                 : Trypsin

Fixed modifications    : Carbamidomethyl (C)

Variable modifications : Deamidation (NQ),Oxidation (M)

Mass values            : Monoisotopic

Protein Mass           : Unrestricted

Peptide Mass Tolerance : ± 0.8 Da

Fragment Mass Tolerance: ± 0.4 Da

Max Missed Cleavages   : 1

Instrument type        : ESI-QUAD-TOF

Number of queries      : 9

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
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Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 2
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Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 3
Score greater than 16 indicates identity
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Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 4
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 5
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 6
Score greater than 15 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein     Peptide
  3.5   -0.09    4  SPAC664.14  K.LKSHMR.I
  2.4   -0.17                   K.KISQALK.K
  2.4   -0.13    6  SPAC17H9.01 R.KISTPNK.L
Top scoring peptide matches to query 7
Score greater than 14 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein     Peptide
  1.3   -0.05    9  SPAC3H8.09c K.ANYGFVQFFTNEDCAR.A
Top scoring peptide matches to query 8
Score greater than 24 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 71.0   -0.20    1  SPAC13G6.10c R.GLAWIPGTDLGYSDNFVNK.G
  7.2   -0.21    3  SPBP23A10.08 K.SKNPVEIGQPANCELRPR.D
  7.2   -0.21    3  SPBP23A10.08 K.SKNPVEIGQPANCELRPR.D
  3.8   -0.21    3  SPBP23A10.08 K.SKNPVEIGQPANCELRPR.D
  3.8   -0.23    7  SPCC63.03    R.LGQKISLPIMWCSVFDR.S
  3.3   -0.26    8  SPAC16E8.04c R.SALIRQEDTIIFNFLER.A
  3.3   -0.24   10  SPBC56F2.04  K.QLNMQRYDSFIKPSLPK.F
  3.2   -0.21    5  SPBC16H5.02  R.GWLAEGGTLIGTARCMAFR.E
  3.1    0.77    3  SPBP23A10.08 K.SKNPVEIGQPANCELRPR.D
  3.1    0.77    3  SPBP23A10.08 K.SKNPVEIGQPANCELRPR.D
Top scoring peptide matches to query 9
Score greater than 22 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
  9.1   -0.25    2  SPCC285.14 K.LFDSSIFSDYLLFFLQK.E