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MS data file    : cw0342_q3.pkl

Database        : spombe  (5049 sequences; 2391832 residues)

Timestamp       : 27 Oct 2006 at 15:59:55 GMT

Significant hits: mSPAC13G6.10c  |predicted| probably mild-alkali sensitive

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 37 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    mSPAC13G6.10c                     Mass: 52568    Score: 54     Queries matched: 1
 |predicted| probably mild-alkali sensitive
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   1033.92   2065.82   2066.01   -0.19 0  54  0.002 1  R.GLAWIPGTDLGYSDNFVNK.G

 
      Proteins matching the same set of peptides:
      SPAC13G6.10c                      Mass: 54406    Score: 52     Queries matched: 1
 |||serine-rich protein|Schizosaccharomyces pombe|chr 1|||Manual

2.    SPBC13E7.11                       Mass: 33556    Score: 14     Queries matched: 1
 ||SPBC30D10.19c|rhomboid family protease|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   762.37   761.37   761.43   -0.06 0  14  13 2  A.SLLHHR.L

3.    SPCC18.06c                        Mass: 37321    Score: 14     Queries matched: 1
 |||CCR4-Not complex |Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   842.43   841.43   841.41   0.02 0  14  11 1  N.FANSTFR.F

4.    SPAC20G4.03c                      Mass: 79983    Score: 14     Queries matched: 1
 |hri1||serine/threonine protein kinase|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   842.43   841.43   841.38   0.04 0  14  11 1  R.SFSTSSNI.F

5.    SPBC12D12.05c                     Mass: 47826    Score: 13     Queries matched: 1
 |||mitochondrial transporter|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 9   1033.92   2065.82   2066.06   -0.24 1  14  17 2  T.IQSVLPCDYSLFKNNLR.V

6.    SPBC16G5.17                       Mass: 66042    Score: 13     Queries matched: 1
 |||transcriptional regulator |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   842.43   841.43   841.43   -0.00 0  14  11 1  K.AIHTSTVN.E

7.    SPBC1709.04c                      Mass: 19122    Score: 12     Queries matched: 1
 |cyp3||cyclophilin |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   762.37   761.37   761.35   0.02 0  15  13 1  K.NSTFHR.I + Deamidation (NQ)

8.    SPAC1006.06                       Mass: 131767   Score: 12     Queries matched: 1
 |rgf2||GDP-GTP exchange protein for Rho1p |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   762.37   761.37   761.38   -0.01 0  14  13 2  D.VTYSHR.L

9.    SPBC4B4.07c                       Mass: 28322    Score: 10     Queries matched: 1
 |||small nuclear ribonucleoprotein |Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 9   1033.92   2065.82   2066.06   -0.24 1  12  31 4  R.ALKDLQGYPLYGKPMMIQ.Y + Deamidation (NQ)

10.   SPCC1235.13                       Mass: 59992    Score: 9      Queries matched: 1
 |ght6|meu12|meiotic expression upregulated|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 9   1033.92   2065.82   2066.04   -0.22 0  13  23 3  I.GALSVIAPGYQSESSPPHIR.G + Deamidation (NQ)

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
10   1041.93   2081.84   2082.17   -0.33 1  10  39 1  GISHVNALLDAIITLFQKK + 2 Deamidation (NQ)
1   352.95   351.95        
2   393.17   392.16        
3   437.03   436.02        
4   487.12   486.11        
5   672.34   671.33        
6   743.36   742.36        

Search Parameters

Type of search         : MS/MS Ion Search

Enzyme                 : semiTrypsin

Fixed modifications    : Carbamidomethyl (C)

Variable modifications : Deamidation (NQ),Oxidation (M)

Mass values            : Monoisotopic

Protein Mass           : Unrestricted

Peptide Mass Tolerance : ± 0.8 Da

Fragment Mass Tolerance: ± 0.4 Da

Max Missed Cleavages   : 1

Instrument type        : ESI-QUAD-TOF

Number of queries      : 10

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
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Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 2
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Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 3
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 4
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 5
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 6
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 7
Score greater than 23 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 14.8    0.02    7  SPBC1709.04c K.NSTFHR.I
 14.5   -0.06    2  SPBC13E7.11  A.SLLHHR.L
 14.5   -0.01    8  SPAC1006.06  D.VTYSHR.L
  9.9   -0.03                    A.AMARGTR.Q
  9.9   -0.02                    C.AMDAKAR.S
  9.9    0.02                    T.AMQANAR.T
  9.9    0.02                    T.AMQANAR.T
  9.9   -0.05                    A.AMSVAKR.V
  9.9   -0.02                    N.AMVQSAR.E
  9.9    0.00                    I.DSANLSR.G
Top scoring peptide matches to query 8
Score greater than 16 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 14.0   -0.00    6  SPBC16G5.17  K.AIHTSTVN.E
 14.0    0.02    3  SPCC18.06c   N.FANSTFR.F
 14.0    0.04    4  SPAC20G4.03c R.SFSTSSNI.F
  2.4   -0.04                    L.DKIPVDR.V
  2.4   -0.00                    K.EIPNVNR.E
  2.4   -0.00                    M.GIPANVDR.E
  2.4   -0.08                    Y.IINLVNR.I
  2.4   -0.04                    R.KPNIVRD.L
  2.4   -0.03                    K.LENPVSAI.K
  2.4   -0.11                    R.LLRAVIAS.P
Top scoring peptide matches to query 9
Score greater than 30 indicates homology
Score greater than 39 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 53.5   -0.19    1  mSPAC13G6.10c R.GLAWIPGTDLGYSDNFVNK.G
 14.2   -0.24    5  SPBC12D12.05c T.IQSVLPCDYSLFKNNLR.V
 12.9   -0.22   10  SPCC1235.13   I.GALSVIAPGYQSESSPPHIR.G
 11.6   -0.24    9  SPBC4B4.07c   R.ALKDLQGYPLYGKPMMIQ.Y
  7.7   -0.19                     R.LGAGEITTRITTDTNFIQD.G
  7.7   -0.19                     R.LGAGEITTRITTDTNFIQD.G
  7.5    0.76   10  SPCC1235.13   I.GALSVIAPGYQSESSPPHIR.G
  6.3   -0.20                     K.SKNPVEIGQPANCELRPR.D
  6.3   -0.20                     K.SKNPVEIGQPANCELRPR.D
  5.8    0.78                     A.KELGYSLIEWLNPMNLK.E
Top scoring peptide matches to query 10
Score greater than 21 indicates homology
Score greater than 39 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 10.4   -0.33        N.GISHVNALLDAIITLFQKK.L
  8.3   -0.24        S.LMQHHMVLYVTVIENVR.E
  8.1   -0.02        M.NAENTFSILNTNEPNAGGSQ.V
  7.7   -0.23        K.LFDSSIFSDYLLFFLQK.E
  7.7   -0.11        R.LYYDGSSMIFANGKMLAQG.H
  6.2   -0.09        K.CNIAGKPVACATQMLESMT.Y
  5.9   -0.10        H.SPFNDTNTLETKLQSDDR.G
  5.2   -0.10        H.SPFNDTNTLETKLQSDDR.G
  4.6   -0.18        T.CSITVDEDEAVIQYALKK.R
  4.3   -0.12        V.PVYTTNTGCPIFNPMAAAR.V