Mascot Search Results

User            : 
Email           : 
Search title    : NaOH released
MS data file    : cw0342_q2.pkl
Database        : spombe  (5049 sequences; 2391832 residues)
Timestamp       : 23 Sep 2006 at 13:55:49 GMT
Significant hits: K1CJ_HUMAN    Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10).
                  mPSU1         |predicted|: Predicted GPI protein : CELL WALL SYNTHESIS PROTEIN
                  K1CJ_BOVIN    Keratin, type I cytoskeletal VIB (Cytokeratin VIB).
                  SPAC13G6.10c  |||serine-rich protein|Schizosaccharomyces pombe|chr 1|||Manual

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 29 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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1.    K1CJ_HUMAN                        Mass: 59711    Score: 117    Queries matched: 3
 Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10).
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   583.24   1164.46   1164.58   -0.11 0  64  1.6e-005 1  R.LENEIQTYR.S
9   617.78   1233.55   1233.67   -0.13 1  32  0.031 1  R.LKYENEVALR.Q
10   683.26   1364.51   1364.63   -0.12 0  21  0.37 1  R.SQYEQLAEQNR.K

2.    mPSU1                             Mass: 40375    Score: 99     Queries matched: 2
 |predicted|: Predicted GPI protein : CELL WALL SYNTHESIS PROTEIN
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   565.72   1129.43   1129.54   -0.11 0  63  2.7e-005 1  K.GTSAQYYVNK.A
12   795.28   1588.55   1588.71   -0.16 0  37  0.0089 1  K.TQWPSTQPSDGETR.G

 
      Proteins matching the same set of peptides:
      SPAC1002.13c                      Mass: 42295    Score: 99     Queries matched: 2
 |psu1||beta-glucosidase |Schizosaccharomyces pombe|chr 1|||Manual
3.    K1CJ_BOVIN                        Mass: 54986    Score: 85     Queries matched: 2
 Keratin, type I cytoskeletal VIB (Cytokeratin VIB).
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   583.24   1164.46   1164.58   -0.11 0  64  1.6e-005 1  R.LENEIQTYR.S
 10   683.26   1364.51   1364.67   -0.16 1  21  0.37 1  R.SQYEQLAEKNR.R

4.    SPAC13G6.10c                      Mass: 54406    Score: 48     Queries matched: 1
 |||serine-rich protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
13   1033.90   2065.79   2066.01   -0.22 0  48  0.00048 1  R.GLAWIPGTDLGYSDNFVNK.G

 
      Proteins matching the same set of peptides:
      mSPAC13G6.10c                     Mass: 52568    Score: 48     Queries matched: 1
 |predicted| probably mild-alkali sensitive
5.    K1CL_HUMAN                        Score: 16     Queries matched: 1
 Keratin, type I cytoskeletal 12 (Cytokeratin 12) (K12) (CK 12).
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   583.24   1164.46   1164.60   -0.14 0  16  0.97 2  R.LELEIETYR.R

 
      Proteins matching the same set of peptides:
      K1CL_RABIT                        Score: 16     Queries matched: 1
6.    SPAC977.09c                       Mass: 75062    Score: 14     Queries matched: 1
 |||lysophospholipase |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   523.23   1044.45   1044.59   -0.14 1  14  2.5 1  R.ISKVNSELR.S

 
      Proteins matching the same set of peptides:
      SPBC1348.10c                      Mass: 75062    Score: 14     Queries matched: 1
 ||SPAC1348.10c|lysophospholipase |Schizosaccharomyces pombe|chr 2|||Manual
7.    SPBC1709.06                       Mass: 54025    Score: 12     Queries matched: 1
 |||dihydrouridine synthase |Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   428.73   855.44   855.44   -0.00 0  12  2.8 1  R.AITVHCR.T

8.    SPAC823.04                        Mass: 22775    Score: 11     Queries matched: 1
 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   523.23   1044.45   1044.59   -0.14 1  11  5.2 2  R.AESIRQTLK.S

9.    SPCC338.13                        Score: 9      Queries matched: 1
 |||Golgi transport complex |Schizosaccharomyces pombe|chr 3|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   428.73   855.44   855.57   -0.13 1  12  2.9 2  R.LVVTRLR.S

10.   SPAC1952.11c                      Score: 9      Queries matched: 1
 |ure2||urease |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   523.23   1044.45   1044.60   -0.15 2  9  7.1 3  K.RVEAVKSTR.N

11.   SPAC18B11.06                      Score: 9      Queries matched: 1
 |||U3 snoRNP component |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 9   617.78   1233.55   1233.71   -0.16 1  9  5.7 2  R.IQYSVDKLLR.A

12.   SPAPB1E7.04c                      Score: 9      Queries matched: 1
 |||glycosyl hydrolase family 18|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   565.72   1129.43   1129.54   -0.11 0  9  7.1 2  K.GTNTAYSAFAK.R

13.   SPBC30B4.02c                      Mass: 79232    Score: 9      Queries matched: 1
 |||R3H domain|Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   435.74   869.46   870.54   -1.08 2  9  5.7 1  R.KAGKAVAAR.M

14.   SPCC1259.14c                      Score: 8      Queries matched: 1
 |meu27|B8647-6|possibly S. pombe specific|Schizosaccharomyces pombe|chr 3|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   428.73   855.44   855.48   -0.04 0  8  6.7 3  K.LAPINNSK.S

15.   SPCC550.02c                       Mass: 40236    Score: 8      Queries matched: 1
 |cwf5|ecm2|RNA-binding protein|Schizosaccharomyces pombe|chr 3|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   435.74   869.46   869.49   -0.03 0  8  7.4 2  K.VQWGKPR.S

16.   SPAC17G6.14c                      Mass: 49656    Score: 7      Queries matched: 1
 |uap56||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   381.70   761.38   761.41   -0.03 0  7  13 1  K.YLPDVR.T

17.   SPBC409.11                        Score: 7      Queries matched: 1
 |meu18||NLS |Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   428.73   855.44   855.59   -0.15 1  7  9.5 4  K.LIKTLIR.K

18.   SPBC19G7.10c                      Mass: 82983    Score: 6      Queries matched: 1
 |||topoisomerase associated protein |Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   421.72   841.43   841.50   -0.06 1  8  6.4 1  K.GMRLLPR.L

19.   SPAPB1E7.01c                      Score: 6      Queries matched: 1
 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   738.32   1474.62   1473.79   0.84 1  6  12 2  -.MEQKVSADVLLNK.Y

20.   SPAC6G9.01c                       Score: 5      Queries matched: 1
 |||hypothetical protein|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   435.74   869.46   870.46   -1.00 1  5  12 3  K.KANPANEK.S

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   738.32   1474.62   1475.83   -1.21 0  6  11 1  LYNAGVVTVLATQK
2   412.72   823.42   823.54   -0.12 1  0  40 1  IVARIPR

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 2 Da
Fragment Mass Tolerance: ± 0.8 Da
Max Missed Cleavages   : 2
Instrument type        : ESI-QUAD-TOF
Number of queries      : 13

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 18 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  7.4   -0.03   16  SPAC17G6.14c K.YLPDVR.T
  5.5    0.92                    K.KQKTTR.L
  5.5    0.96                    K.QKQTTR.T
  3.7   -0.98                    R.GTPETMK.A
  2.3    1.99                    K.VYEPPR.Y
  1.8    0.92                    M.GKAKTTR.K
  1.0    1.91                    R.YVLPLR.E
  0.7    0.95                    R.VYINPR.F
  0.2   -1.01                    R.GGRGGGFR.G
  0.1    1.95                    R.TVASLNR.E
Top scoring peptide matches to query 2
Score greater than 13 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  0.2   -0.12        K.IVARIPR.A
Top scoring peptide matches to query 3
Score greater than 17 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  8.5   -0.06   18  SPBC19G7.10c K.GMRLLPR.L
  4.5    0.97                    K.NVGNPRGK.G
  3.3   -0.11                    K.ISALGLLR.L
  2.4    0.01                    K.VTAEYFL.-
  2.2   -0.07                    K.EKSLIPR.R
  2.2   -0.04                    R.ENTLLPR.L
  0.6   -0.10                    K.LGILTPTK.A
Top scoring peptide matches to query 4
Score greater than 22 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 12.2   -0.00    7  SPBC1709.06  R.AITVHCR.T
 11.9   -0.13    9  SPCC338.13   R.LVVTRLR.S
  8.4   -0.04   14  SPCC1259.14c K.LAPINNSK.S
  6.8   -0.15   17  SPBC409.11   K.LIKTLIR.K
  4.7   -0.08                    K.INDLILR.A
  4.3   -0.12                    K.IKDLVLR.C
  3.8   -0.02                    K.IVGNSNPR.E
  3.1   -1.08                    K.SLNAALLR.Y
  2.9   -0.06                    K.MPNLILR.N
  2.3   -1.11                    K.GLAKIISR.L
Top scoring peptide matches to query 5
Score greater than 21 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  8.7   -1.08   13  SPBC30B4.02c R.KAGKAVAAR.M
  7.5   -0.03   15  SPCC550.02c  K.VQWGKPR.S
  5.4   -1.11                    K.KAIGNIKK.F
  5.4   -1.00   20  SPAC6G9.01c  K.KANPANEK.S
  5.4   -1.11                    K.KAWILLK.E
  5.4   -1.06                    K.QANKVRR.Q
  5.2   -0.05                    K.DRLTIPR.R
  4.5   -0.06                    R.VVRNNLR.V
  4.5   -1.98                    K.EASPVNQK.L
  3.9   -1.09                    R.VVRVRSR.E
Top scoring peptide matches to query 6
Score greater than 24 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 13.8   -0.14    6  SPAC977.09c  R.ISKVNSELR.S
 10.6   -0.14    8  SPAC823.04   R.AESIRQTLK.S
  9.2   -0.15   10  SPAC1952.11c K.RVEAVKSTR.N
  8.2   -1.05                    K.NSNELENVK.R
  1.9   -0.20                    R.SLSLLKVASK.S
  1.4   -0.11                    R.EASKALDRR.Q
  0.9    0.78                    K.KASLSKLGLK.N
Top scoring peptide matches to query 7
Score greater than 25 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 62.9   -0.11    2  mPSU1        K.GTSAQYYVNK.A
  8.7   -0.11   12  SPAPB1E7.04c K.GTNTAYSAFAK.R
  6.2    0.77                    R.VGAAKKISNNK.A
  3.8    0.79                    K.YIRVLSHNK.K
  3.1    0.74                    R.VTRSLVLVDK.T
  2.9   -0.27                    K.ILRTMAKGLK.L
  2.6   -0.17                    R.QSVEADIAGLK.R
  2.0    1.80                    K.GKKATSTPNPK.S
  1.9    1.91                    R.SATSNFSTSAR.F
  1.9   -1.19                    R.SSIEGVLADLK.L
Top scoring peptide matches to query 8
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 64.2   -0.11    1+ K1CJ_HUMAN R.LENEIQTYR.S
 16.5   -0.14    5  K1CL_HUMAN R.LELEIETYR.R
  6.1   -1.10                  R.LENEIETYR.R
  5.5    0.77                  K.ENILIPLPQK.R
  3.7   -0.26                  R.NQLGVAPIILK.H
  3.4    0.83                  K.ELNITSFGKR.F
  3.2    0.76                  R.SIAGRVHRLR.L
  2.9    0.81                  R.TTGLLDLYLR.L
  2.4    0.87                  K.LAHYLQYEK.K
  2.2   -1.18                  M.SSSTYRKLPK.I
Top scoring peptide matches to query 9
Score greater than 24 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 31.8   -0.13    1  K1CJ_HUMAN   R.LKYENEVALR.Q
  9.1   -0.16   11  SPAC18B11.06 R.IQYSVDKLLR.A
  6.5    1.87                    K.KKTSTGSALDPK.K
  3.3    1.90                    K.GVVVDVESMGLK.E
  3.3   -1.15                    R.KLSMSSKVDIK.A
  3.3   -1.15                    K.QILTNVSGYLK.K
  1.9   -0.16                    R.IAGNLQLYSKK.R
  1.6   -0.16                    K.KLSDKFVEIR.S
  1.3   -1.96                    K.DQEANNSSDTR.K
  0.8    0.92                    K.EKELEASKDGK.I
Top scoring peptide matches to query 10
Score greater than 15 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 20.6   -0.16    3  K1CJ_BOVIN R.SQYEQLAEKNR.R
 20.6   -0.12    1  K1CJ_HUMAN R.SQYEQLAEQNR.K
  1.4   -0.17                  K.KHLASFSSEGFR.T
Top scoring peptide matches to query 11
Score greater than 14 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  6.0   -1.21                    K.LYNAGVVTVLATQK.G
  5.8    0.84   19  SPAPB1E7.01c -.MEQKVSADVLLNK.Y
  1.6   -1.16                    R.KELDHFTSKPFK.N
  1.0   -1.14                    -.MEVGSLEVKKNDK.D
  0.7   -0.08                    K.ENQYAASEHSVLK.K
Top scoring peptide matches to query 12
Score greater than 16 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 36.5   -0.16    2  mPSU1 K.TQWPSTQPSDGETR.G
  1.6   -1.27             R.QTLLSHNEFLNFK.N
  0.9    0.54             K.AKLILIAANAPPLRK.S
Top scoring peptide matches to query 13
Score greater than 17 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 48.2   -0.22    4  SPAC13G6.10c R.GLAWIPGTDLGYSDNFVNK.G
  2.0   -1.32                    K.KLDAINRLSNIIYYSER.V
  1.8   -0.35                    R.IIAVSGMSDLKWELHVIR.D
  1.7   -0.39                    R.LKALLVLSIENTENPEKR.K
  1.0    1.55                    K.LVASVLALMTIVPAQAGLIGK.R
  0.4   -0.18                    K.DHEYSLQLQSYREAAEK.A
  0.3   -0.39                    R.LALSRFLEKPIRYFTGR.T