Mascot Search Results

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MS data file    : cw0342_q1.pkl
Database        : spombe  (5049 sequences; 2391832 residues)
Timestamp       : 23 Sep 2006 at 13:10:18 GMT
Significant hits: mPSU1  |predicted|: Predicted GPI protein : CELL WALL SYNTHESIS PROTEIN

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 30 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    mPSU1                             Mass: 40375    Score: 59     Queries matched: 2
 |predicted|: Predicted GPI protein : CELL WALL SYNTHESIS PROTEIN
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   565.70   1129.38   1129.54   -0.16 0  39  0.0064 1  K.GTSAQYYVNK.A
8   795.25   1588.48   1588.71   -0.23 0  20  0.4 1  K.TQWPSTQPSDGETR.G

 
      Proteins matching the same set of peptides:
      SPAC1002.13c                      Mass: 42295    Score: 59     Queries matched: 2
 |psu1||beta-glucosidase |Schizosaccharomyces pombe|chr 1|||Manual

2.    SPBC1709.06                       Mass: 54025    Score: 17     Queries matched: 1
 |||dihydrouridine synthase |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   428.71   855.40   855.44   -0.04 0  17  0.85 1  R.AITVHCR.T

3.    SPAC823.04                        Mass: 22775    Score: 11     Queries matched: 1
 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   523.22   1044.42   1044.59   -0.18 1  11  4.5 1  R.AESIRQTLK.S

4.    SPCC330.06c                       Mass: 16722    Score: 10     Queries matched: 1
 |pmp20||peroxiredoxin|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   435.72   869.42   870.52   -1.10 0  10  4.3 1  M.VAVGSTLPK.V

5.    SPAC3H5.09c                       Mass: 312425   Score: 9      Queries matched: 2
 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   565.70   1129.38   1127.64   1.74 1  1  44 2  R.ELILGASNRR.K
7   738.29   1474.57   1475.73   -1.15 0  14  1.7 1  R.SGNLFPTLDSSPNK.K

6.    SPAPB1E7.01c                      Score: 8      Queries matched: 1
 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   738.29   1474.57   1473.79   0.79 1  8  7.8 2  -.MEQKVSADVLLNK.Y

7.    SPBCPT2R1.08c                     Score: 6      Queries matched: 1
 |||helicase|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   435.72   869.42   869.54   -0.12 1  6  12 2  K.VVRDIIR.T

 
      Proteins matching the same set of peptides:
      SPAC212.11                        Score: 6      Queries matched: 1
8.    SPAC6G9.01c                       Score: 6      Queries matched: 1
 |||hypothetical protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   435.72   869.42   870.46   -1.03 1  6  12 3  K.KANPANEK.S

9.    SPBC30B4.02c                      Score: 6      Queries matched: 1
 |||R3H domain|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   435.72   869.42   870.54   -1.12 2  6  12 3  R.KAGKAVAAR.M

10.   SPAC1834.10c                      Score: 6      Queries matched: 1
 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   435.72   869.42   870.57   -1.14 2  6  12 3  K.KAIGNIKK.F

11.   SPAC20H4.01                       Score: 5      Queries matched: 1
 ||SPAC631.03|WD repeat protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   428.71   855.40   855.51   -0.11 0  5  14 2  K.LALRPMR.A

12.   SPAC637.11                        Score: 5      Queries matched: 1
 |suv3||RNA helicase ATP-dependent|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   795.25   1588.48   1588.96   -0.47 2  5  12 2  K.TKAIAEAFLIKGISK.L

13.   TRY2_CANFA                        Score: 5      Queries matched: 1
 Trypsin, anionic precursor (EC 3.4.21.4).
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   428.71   855.40   855.52   -0.11 0  5  14 3  R.VATISLPR.A

14.   SPAC1B3.09c                       Score: 4      Queries matched: 1
 |||involved in ribosome biogenesis and assembly |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   428.71   855.40   855.43   -0.03 1  4  17 6  K.NRNSHTK.N

15.   SPCC825.02                        Score: 4      Queries matched: 1
 |||cell surface signalling protein |Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   795.25   1588.48   1589.74   -1.26 1  4  15 3  R.DLDAAEENEKSLEK.E

16.   SPCC550.02c                       Mass: 40236    Score: 4      Queries matched: 1
 |cwf5|ecm2|RNA-binding protein|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   435.72   869.42   869.49   -0.07 0  4  17 6  K.VQWGKPR.S

17.   SPCC757.03c                       Score: 3      Queries matched: 1
 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   795.25   1588.48   1587.89   0.59 2  3  22 4  K.STAEAAIKVSQSLRK.T

18.   SPBC25H2.11c                      Score: 2      Queries matched: 1
 |||bromodomain protein|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   795.25   1588.48   1589.93   -1.44 2  2  22 5  R.IASSVFLNQSLRKK.R

19.   SPAC26F1.01                       Score: 2      Queries matched: 1
 |sec74|SPAPJ691.01c|Sec7 domain|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   795.25   1588.48   1587.86   0.62 2  2  22 5  K.KIDSVRTSIESPTR.T

20.   SPAC4F8.11                        Score: 2      Queries matched: 1
 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   795.25   1588.48   1589.76   -1.28 2  2  23 7  K.ATGNEQSEKGKENAK.T

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   381.68   761.35   761.41   -0.06 0  2  45 1  YLPDVR
2   421.70   841.39   841.50   -0.11 0  1  39 1  VDNILLR

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 2 Da
Fragment Mass Tolerance: ± 0.8 Da
Max Missed Cleavages   : 2
Instrument type        : ESI-QUAD-TOF
Number of queries      : 8

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 14 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.1   -0.06        K.YLPDVR.T
  0.9    1.96        K.VYEPPR.Y
Top scoring peptide matches to query 2
Score greater than 13 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  0.8   -0.11        R.VDNILLR.S
Top scoring peptide matches to query 3
Score greater than 19 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein     Peptide
 17.4   -0.04    2  SPBC1709.06 R.AITVHCR.T
  5.2   -0.11   11  SPAC20H4.01 K.LALRPMR.A
  5.1   -0.11   13  TRY2_CANFA  R.VATISLPR.A
  4.9   -1.12                   K.REVVKAR.R
  4.9    0.87                   R.REVVPKK.E
  4.4   -0.03   14  SPAC1B3.09c K.NRNSHTK.N
  3.0   -0.13                   K.RDLLLAR.E
  1.2    0.89                   K.ILRNSPR.A
  0.7   -0.15                   K.LGKAIVQK.R
  0.5   -0.11                   K.LALLAAER.K
Top scoring peptide matches to query 4
Score greater than 19 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
  9.9   -1.10    4  SPCC330.06c   M.VAVGSTLPK.V
  5.6   -0.12    7  SPBCPT2R1.08c K.VVRDIIR.T
  5.5   -1.12    9  SPBC30B4.02c  R.KAGKAVAAR.M
  5.5   -1.14   10  SPAC1834.10c  K.KAIGNIKK.F
  5.5   -1.03    8  SPAC6G9.01c   K.KANPANEK.S
  3.9   -0.07   16  SPCC550.02c   K.VQWGKPR.S
  3.6   -1.16                     K.VTRVIRK.T
  2.5   -1.00                     K.QSPASPER.D
  2.1   -1.11                     M.KNDLILR.A
  2.1   -1.07                     K.QENVLLR.K
Top scoring peptide matches to query 5
Score greater than 19 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 11.2   -0.18    3  SPAC823.04 R.AESIRQTLK.S
  6.2   -1.09                  K.NSNELENVK.R
  1.1   -0.16                  K.ILENSVKDK.F
  1.1   -1.10                  R.SLPEMISNR.K
  0.9   -0.18                  R.ISKVNSELR.S
  0.8   -0.24                  R.SLSLLKVASK.S
  0.6   -0.15                  R.EASKALDRR.Q
  0.6    0.75                  K.KASLSKLGLK.N
Top scoring peptide matches to query 6
Score greater than 16 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein     Peptide
 39.2   -0.16    1  mPSU1       K.GTSAQYYVNK.A
  0.8    1.74    5  SPAC3H5.09c R.ELILGASNRR.K
  0.7   -0.32                   K.ILRTMAKGLK.L
  0.7   -1.22                   R.SNNGLSILASR.L
  0.5   -1.23                   K.SRLGLNRCR.A
Top scoring peptide matches to query 7
Score greater than 21 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 14.2   -1.15    5  SPAC3H5.09c  R.SGNLFPTLDSSPNK.K
  7.5    0.79    6  SPAPB1E7.01c -.MEQKVSADVLLNK.Y
  3.8   -1.26                    K.LYNAGVVTVLATQK.G
  3.0   -1.15                    R.FTAADLAAVPEESR.K
  1.6   -0.19                    K.VSATENSAPKKTDK.E
  0.5    0.79                    K.EPTWKVDLVPYK.Q
Top scoring peptide matches to query 8
Score greater than 19 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 19.9   -0.23    1  mPSU1        K.TQWPSTQPSDGETR.G
  5.2   -0.47   12  SPAC637.11   K.TKAIAEAFLIKGISK.L
  4.2   -1.26   15  SPCC825.02   R.DLDAAEENEKSLEK.E
  2.5    0.59   17  SPCC757.03c  K.STAEAAIKVSQSLRK.T
  2.5   -1.44   18  SPBC25H2.11c R.IASSVFLNQSLRKK.R
  2.5    0.62   19  SPAC26F1.01  K.KIDSVRTSIESPTR.T
  2.5   -1.28   20  SPAC4F8.11   K.ATGNEQSEKGKENAK.T
  2.4    0.64                    R.LDERFIDANGVAIR.D
  2.4    0.66                    K.LLVLFDSEDPRER.D
  2.4    1.61                    K.NIRTELSDISGVRK.G