Mascot Search Results

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MS data file    : cw0341_q4.pkl

Database        : spombe  (5049 sequences; 2391832 residues)

Timestamp       : 27 Oct 2006 at 13:49:27 GMT

Significant hits: mSPAC13G6.10c  |predicted| probably mild-alkali sensitive

                  SPAC11E3.13c   |||GPI anchored protein |Schizosaccharomyces pombe|chr 1|||Manual

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 38 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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        Error tolerant   

1.    mSPAC13G6.10c                     Mass: 52568    Score: 212    Queries matched: 3
 |predicted| probably mild-alkali sensitive
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
21   945.87   1889.72   1889.93   -0.21 0  115  1.4e-009 1  K.TLMTSALGFLDGHGSVER.Y
22   953.88   1905.74   1905.93   -0.18 0  (61) 0.00036 1  K.TLMTSALGFLDGHGSVER.Y + Oxidation (M)
23   1033.91   2065.81   2066.01   -0.20 0  97  9.4e-008 1  R.GLAWIPGTDLGYSDNFVNK.G

 
      Proteins matching the same set of peptides:
      SPAC13G6.10c                      Mass: 54406    Score: 211    Queries matched: 3
 |||serine-rich protein|Schizosaccharomyces pombe|chr 1|||Manual

2.    SPAC11E3.13c                      Mass: 54058    Score: 84     Queries matched: 1
 |||GPI anchored protein |Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
25   1190.48   2378.95   2379.13   -0.17 0  85  1.3e-006 1  R.GVDYQPGGSSTLVDPLADTSICK.R

 
      Proteins matching the same set of peptides:
      mSPAC11E3.13C                     Mass: 51689    Score: 84     Queries matched: 1
 |predicted|: Predicted GPI protein : Gas1 homology
3.    mSPAC1705.03C                     Mass: 41468    Score: 19     Queries matched: 1
 |predicted|: Predicted GPI protein : Ecm33 family
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
14   837.36   836.35   836.44   -0.09 0  19  4.7 1  K.GVSVNFSK.L

 
      Proteins matching the same set of peptides:
      SPAC1705.03c                      Mass: 43547    Score: 17     Queries matched: 1
 ||SPAC23H4.19|involved in cell wall biosynthesis |Schizosaccharomyces pombe|chr 1|||Manual
4.    SPAC23D3.10c                      Mass: 78240    Score: 18     Queries matched: 1
 |eng2||glycosyl hydrolase family 81|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 21   945.87   1889.72   1889.99   -0.27 0  20  4.8 2  R.AHPSPPLGLTSNLFPIQT.N + Deamidation (NQ)

5.    SPAC56F8.03                       Mass: 120371   Score: 17     Queries matched: 1
 |||translation initiation factor if-2 |Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
13   818.38   817.37   817.42   -0.05 0  20  4.1 1  N.NEVVTEK.K

6.    SPACUNK4.07c                      Mass: 137542   Score: 16     Queries matched: 1
 |cta4|sev4, SPAPYUK71.01|P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 14   837.36   836.35   836.45   -0.10 1  18  5.7 2  V.RVSGGSFK.T

7.    SPBC27.04                         Mass: 118988   Score: 16     Queries matched: 1
 |||Mre11 complex |Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 13   818.38   817.37   817.38   -0.01 0  20  4.4 2  S.NVEEAEK.D

8.    b1,3glucanase                     Mass: 54953    Score: 16     Queries matched: 1
 min sig.pept ACC M38734|O.xanthineolytica 512 aa
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
15   842.43   841.42   841.44   -0.02 1  16  6.7 1  K.ALRNPQSG.K

9.    SPCC1682.08c                      Mass: 79049    Score: 16     Queries matched: 1
 |||RNA-binding protein|Schizosaccharomyces pombe|chr 3|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 13   818.38   817.37   817.42   -0.05 0  17  7.7 4  D.NVTEDIK.L

10.   SPBC23G7.10c                      Mass: 43770    Score: 16     Queries matched: 1
 |||NADH-dependent flavin oxidoreductase |Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 13   818.38   817.37   817.43   -0.06 0  17  8.1 5  Y.QVPFAEK.I

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   672.34   671.33   671.41   -0.08 1  14  11 1  RDIIR
17   1253.50   1252.49   1252.58   -0.09 1  13  19 1  LRFSGSMVQNP + 2 Deamidation (NQ); Oxidation (M)
4   487.12   486.11   486.24   -0.13 0  12  3.3 1  SGQPV
12   811.20   810.19   810.42   -0.23 0  12  28 1  ADHILVSG
10   762.38   761.37   761.40   -0.04 1  10  41 1  SRAETAK
24   1092.95   2183.88   2184.18   -0.30 1  9  57 1  FVIGQIPGHMLLQRFPVSK + 2 Deamidation (NQ); Oxidation (M)
20   921.90   1841.78   1842.04   -0.26 1  7  78 1  GRTGTFIAVDQILQVPK
5   541.26   540.25   540.18   0.06 0  7  10 1  MNSSS + Oxidation (M)
11   811.19   810.18   809.41   0.77 1  5  1.2e+002 1  YRIDLM
1   437.19   436.18        
2   487.11   486.10        
3   487.11   486.11        
6   561.23   560.22        
7   649.15   648.15        
8   649.15   648.15        
16   973.24   972.23        
18   1297.34   1296.33        
19   1459.40   1458.39        

Search Parameters

Type of search         : MS/MS Ion Search

Enzyme                 : semiTrypsin

Fixed modifications    : Carbamidomethyl (C)

Variable modifications : Deamidation (NQ),Oxidation (M)

Mass values            : Monoisotopic

Protein Mass           : Unrestricted

Peptide Mass Tolerance : ± 0.8 Da

Fragment Mass Tolerance: ± 0.4 Da

Max Missed Cleavages   : 1

Instrument type        : ESI-QUAD-TOF

Number of queries      : 25

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 2
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 3
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 4
Score greater than 24 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 11.5   -0.13        K.SGQPV.L
 10.8   -0.13        K.GSGPAV.A
  8.5   -0.14        R.GSRPA.I
  7.9   -0.13        K.TPNGV.E
Top scoring peptide matches to query 5
Score greater than 19 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  6.7    0.06        MNSSS
  6.7    0.03        K.NFSSS.N
Top scoring peptide matches to query 6
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 7
Score greater than 39 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 8
Score greater than 39 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 9
Score greater than 25 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 14.2   -0.08        RDIIR
 14.2   -0.08        RDILR
 14.2   -0.08        RDLIR
 14.2   -0.08        RDLLR
 14.2   -0.08        RNIIR
 14.2   -0.08        A.RNILR.N
 14.2   -0.08        RNLIR
 14.2   -0.08        RNLLR
 13.7   -0.07        E.SSLLPR.L
 11.4    0.00        P.VGDQPR.D
Top scoring peptide matches to query 10
Score greater than 21 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  9.6   -0.04        G.SRAETAK.N
  9.4   -0.04        Q.YLQNPK.S
  8.2   -0.02        R.SSKTNPK.R
  5.5   -0.06        S.SSKELAK.Q
  5.3   -0.04        M.YLVNPR.D
  4.7    0.01        R.SSELNVN.E
  4.7   -0.06        I.SSSVIAAK.L
  4.3   -0.09        N.SRKPFK.D
  4.3   -0.02        L.SNLQTAK.E
  4.3   -0.06        R.STKVQAK.I
Top scoring peptide matches to query 11
Score greater than 18 indicates homology
Score greater than 39 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  5.2    0.77        K.YRIDLM.E
  5.1    0.78        R.MSSLLLM.C
Top scoring peptide matches to query 12
Score greater than 21 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 11.5   -0.23        K.ADHILVSG.H
 11.2   -0.24        M.APRVAPGGS.Q
  8.4   -0.16        R.YGSSAGNVG.D
  3.1   -0.17        T.GSGSNFSR.A
Top scoring peptide matches to query 13
Score greater than 30 indicates homology
Score greater than 39 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 19.9   -0.05    5  SPAC56F8.03  N.NEVVTEK.K
 19.6   -0.01    7  SPBC27.04    S.NVEEAEK.D
 17.2   -0.01                    L.VNEAEEK.N
 17.2   -0.05    9  SPCC1682.08c D.NVTEDIK.L
 16.9   -0.10                    E.KVPFAEK.F
 16.9   -0.06   10  SPBC23G7.10c Y.QVPFAEK.I
 15.9   -0.01                    I.VNDDVEK.A
 15.6   -0.01                    P.NEAEVEK.L
 15.6   -0.05                    R.NSIEVQK.F
 15.1   -0.06                    L.FDNVVPK.T
Top scoring peptide matches to query 14
Score greater than 27 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 18.6   -0.09    3  mSPAC1705.03C K.GVSVNFSK.L
 17.7   -0.10    6  SPACUNK4.07c  V.RVSGGSFK.T
 13.5   -0.02                     R.NVSEKMN.D
 12.6   -0.04                     GSVGESSSK
 12.3   -0.04                     K.SSASPSSSK.S
 11.1   -0.10                     L.FRNSVSK.N
  9.9   -0.11                     D.LTKSSSSK.E
  9.9   -0.05                     L.ENVNFSK.A
  9.6   -0.07                     K.RCLSSSK.Y
  8.8   -0.00                     Y.EQGNSSSK.F
Top scoring peptide matches to query 15
Score greater than 20 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 16.0   -0.02    8  b1,3glucanase K.ALRNPQSG.K
  7.0   -0.09                     S.RLANLGAK.L
  2.2   -0.12                     R.LARLILSG.G
  2.0   -0.09                     I.RSPILTR.Q
  0.6   -0.12                     V.IVKGGQLK.D
  0.2    0.09                     K.DVTEYDT.V
  0.2    0.03                     T.NVQTHNK.I
  0.1   -0.05                     R.VGISPDRV.Q
  0.1   -0.08                     T.VGIGAGQLK.S
Top scoring peptide matches to query 16
Score greater than 40 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 17
Score greater than 25 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 13.0   -0.09        R.LRFSGSMVQNP.H
 12.2   -0.08        K.NNPNQNPQAAAL.T
 12.0    0.64        K.LLKIVLLTAGVI.G
 12.0    0.80        K.QARVLDPNQVL.S
 11.7   -0.02        R.NNADSQSLQSST.E
 11.6   -0.10        R.NVQSHNRGINN.R
 11.6   -0.10        R.NVQSHNRGINN.R
 11.6   -0.10        R.NVQSHNRGINN.R
 11.5   -0.08        V.DDEGRLYTER.L
 11.5   -0.06        K.DNQGLLEGKNY.N
Top scoring peptide matches to query 18
Score greater than 41 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 19
Score greater than 41 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 20
Score greater than 18 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  6.8   -0.26        V.GRTGTFIAVDQILQVPK.N
  5.3   -0.13        P.PAQNQVLPFQSLNAPSK.K
  2.8   -0.13        P.PAQNQVLPFQSLNAPSK.K
  2.8   -0.13        P.PAQNQVLPFQSLNAPSK.K
  2.1   -0.13        G.VYLYFEDNAGVIVNPK.G
  1.3    0.71        D.SIIPLRYQQPTLLLGK.C
  1.1   -0.11        R.DMFQKILQGSVELFNG.K
  1.1    0.72        G.IVTIVNPFRLLGFSHK.Q
  0.8   -0.19        D.QMLFLNQLLLEHNTK.Y
  0.2   -0.11        P.GGSIQDCFLVNGVLFSK.K
Top scoring peptide matches to query 21
Score greater than 39 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
115.3   -0.21    1  mSPAC13G6.10c K.TLMTSALGFLDGHGSVER.Y
 20.0   -0.27    4  SPAC23D3.10c  R.AHPSPPLGLTSNLFPIQT.N
  7.2   -0.23                     E.EAQVVVQTLNGYEINGR.R
  6.6    0.72    4  SPAC23D3.10c  R.AHPSPPLGLTSNLFPIQT.N
  5.2   -0.21                     K.QSDRVAVTASTGLAACNIG.G
  4.8   -0.21                     R.WAFHTGRILGMSWNAK.S
  3.8   -0.15                     R.NAVMNIMLWVPDWDGI.L
  3.2   -0.13                     P.DVENMSFDGKDFPVFK.E
  3.0   -0.23                     S.TICVVLAGSKSDLQNQR.Q
  3.0   -0.23                     S.TICVVLAGSKSDLQNQR.Q
Top scoring peptide matches to query 22
Score greater than 21 indicates homology
Score greater than 39 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 61.1   -0.18    1  mSPAC13G6.10c K.TLMTSALGFLDGHGSVER.Y
  4.3   -0.09                     R.TFLGMAIMSFNQLTGCN.Y
  4.3   -0.09                     R.TFLGMAIMSFNQLTGCN.Y
  4.3   -0.09                     R.TFLGMAIMSFNQLTGCN.Y
  2.8   -0.12                     K.LQLDRNDIDQAMDTQI.D
  2.8   -0.12                     K.LQLDRNDIDQAMDTQI.D
  2.0   -0.07                     R.LTLNCLDTPSDENGEDI.A
  1.9    0.72                     G.TLGGNAVLEIFGQSKLTR.T
  0.5   -0.11                     R.VNDMSPVEGDLGLQLSSE.A
  0.3   -0.19                     L.LFLCSIAVFYYDGPNK.D
Top scoring peptide matches to query 23
Score greater than 32 indicates homology
Score greater than 39 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 97.0   -0.20    1  mSPAC13G6.10c R.GLAWIPGTDLGYSDNFVNK.G
 13.4   -0.25                     T.IQSVLPCDYSLFKNNLR.V
 10.9    0.77                     A.KELGYSLIEWLNPMNLK.E
  9.6   -0.23                     A.QLEAVKLTDTTEVDFTEK.L
  9.6   -0.25                     R.ALKDLQGYPLYGKPMMIQ.Y
  9.1   -0.11                     G.KEPEGYLSNQSNNGSAQNK.K
  9.1   -0.11                     G.KEPEGYLSNQSNNGSAQNK.K
  9.1   -0.11                     G.KEPEGYLSNQSNNGSAQNK.K
  9.1   -0.11                     G.KEPEGYLSNQSNNGSAQNK.K
  9.1   -0.11                     G.KEPEGYLSNQSNNGSAQNK.K
Top scoring peptide matches to query 24
Score greater than 18 indicates homology
Score greater than 39 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  8.7   -0.30        G.FVIGQIPGHMLLQRFPVSK.F
  5.3    0.68        K.GQGFLGVINLRVGDVLDLAIGG.D
  5.3   -0.13        R.QDVSNLVNRGQAQLQHMLN.M
  2.2   -0.23        R.TLIPSLANKIDDQNLQGILD.E
  2.1    0.71        T.AWTWIKPCFFNFVILLK.R
  2.1   -0.24        K.RLNKPFQQQTALPISMPVP.F
  2.0   -0.13        R.QDVSNLVNRGQAQLQHMLN.M
  2.0   -0.24        N.TINIPTDSLSIVSNPSQTPAK.F
  2.0   -0.30        N.KLTHQTLPNVFTQLSELSK.C
  1.6    0.69        G.FVIGQIPGHMLLQRFPVSK.F
Top scoring peptide matches to query 25
Score greater than 23 indicates homology
Score greater than 39 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 85.2   -0.17    2  SPAC11E3.13c R.GVDYQPGGSSTLVDPLADTSICK.R
  5.0   -0.21                    R.LTDRTVFISGLPNELCSTENL.K
  3.6   -0.26                    D.MCIGTPMRLVQAIQTGLSFEK.V
  3.5   -0.16                    K.KYASLEQSSHESQLYLVNPSP.E
  3.3   -0.14                    K.ASSGMQQLKSQLEENIPVNSAM.E
  3.3   -0.11                    K.VHNHINELQGVLSVNCSSSPDA.A
  3.3   -0.11                    K.VHNHINELQGVLSVNCSSSPDA.A
  2.8   -0.26                    L.QAAKSVFQSISGDLVTLAVECR.S
  2.4   -0.27                    F.VASASGVGFGNSAGVEKNKPYALR.T
  2.2   -0.19                    H.PNDHVRNTVQQAIENISNNSK.L