Mascot Search Results

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MS data file    : cw0340_q3.pkl
Database        : spombe  (5049 sequences; 2391832 residues)
Timestamp       : 27 Sep 2006 at 09:39:00 GMT
Significant hits: TRBOTR         trypsin (EC3.4.21.4 ) precursor-bovine
                  b1,6glucanase  | ACCESSION: S55325 |Trichoderma harzianum - 430 aa

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 30 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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        Error tolerant   

1.    TRBOTR                            Mass: 24662    Score: 402    Queries matched: 7
 trypsin (EC3.4.21.4 ) precursor-bovine
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   403.26   804.50   804.41   0.09 0  41  0.0058 1  K.SAASLNSR.V
6   453.81   905.60   905.50   0.10 0  28  0.12 1  K.NKPGVYTK.V
9   539.33   1076.64   1076.52   0.12 0  66  1.7e-005 1  K.APILSDSSCK.S
11   577.35   1152.69   1152.57   0.13 0  60  5.4e-005 1  K.SSGTSYPDVLK.C
12   584.86   1167.70   1167.57   0.13 0  51  0.00043 1  K.VCNYVSWIK.Q
18   745.96   1489.90   1489.73   0.17 0  63  2.9e-005 1  K.LQGIVSWGSGCAQK.N
20   1082.21   2162.40   2162.05   0.36 0  95  1.5e-008 1  R.LGEDNINVVEGNEQFISASK.S

2.    b1,6glucanase                     Mass: 48363    Score: 192    Queries matched: 5
 | ACCESSION: S55325 |Trichoderma harzianum - 430 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   432.76   863.50   863.40   0.09 0  12  4.1 1  K.FQNHYR.D
10   567.37   1132.73   1132.61   0.12 0  16  1.6 1  R.FEPALASGITK.I
13   602.90   1203.78   1203.64   0.14 1  59  6.7e-005 1  K.KLHVQFMSSK.W
14   614.88   1227.75   1227.61   0.14 0  56  0.00015 1  R.DAEAALNVPSNK.K
15   678.94   1355.86   1355.70   0.16 1  49  0.00065 1  R.DAEAALNVPSNKK.L

3.    SPBC83.01                         Score: 22     Queries matched: 2
 |ucp8||actin cortical patch component |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   453.81   905.60   906.52   -0.92 0  13  3.4 4  K.AGDPPILPK.Q
 13   602.90   1203.78   1203.67   0.11 2  9  6.9 5  R.KLEEKIESTK.Y

4.    SPBC19C7.07c                      Mass: 32901    Score: 16     Queries matched: 2
 |||tRNA-splicing endonuclease |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   453.81   905.60   904.54   1.06 2  11  5.2 9  K.KIEIMKK.L + Oxidation (M)
 20   1082.21   2162.40   2162.05   0.35 2  5  16 2  K.KTWLIGGSNDRNINMNGEK.S + Oxidation (M)

5.    SPBC4C3.09                        Score: 15     Queries matched: 1
 |||galactosyltransferase family 8|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 13   602.90   1203.78   1203.67   0.11 2  15  2 2  K.LKHHPETKSK.Y

6.    SPBC30D10.17c                     Score: 14     Queries matched: 1
 |||glucan synthase regulator |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   453.81   905.60   905.47   0.13 0  14  2.7 3  K.EIETTSVK.Q

7.    SPBC13G1.10c                      Score: 14     Queries matched: 1
 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   453.81   905.60   905.52   0.08 1  19  0.89 2  K.ELKELFK.Y

8.    SPBC2G2.11                        Mass: 54062    Score: 12     Queries matched: 1
 |||N-myristoyltransferase 1|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
21   1275.77   2549.52   2551.34   -1.81 2  12  2.3 1  K.KITDFFSFYSLPSTVIGNPKYK.D

9.    SPAC23C4.07                       Score: 12     Queries matched: 1
 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   453.81   905.60   904.47   1.13 0  12  4.2 5  K.QIEQMLK.F + Oxidation (M)

10.   SPBC3E7.05c                       Score: 12     Queries matched: 1
 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   453.81   905.60   904.50   1.10 0  12  4.6 6  R.LQQELFK.L

11.   SPAC16A10.07c                     Score: 11     Queries matched: 1
 |taz1|myb1, myb|involved in telomere maintenance|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   403.26   804.50   804.42   0.08 1  11  4.9 2  R.SAARSGTR.R

12.   SPAC3A12.04c                      Score: 11     Queries matched: 1
 |||RNase P subunit |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   453.81   905.60   905.45   0.15 0  11  5.2 7  K.ELYPEQK.I

13.   SPAC23A1.10                       Score: 11     Queries matched: 1
 |ef1a-b||translation elongation factor |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 13   602.90   1203.78   1203.69   0.10 2  11  4.7 3  K.KIEESPKFVK.S

 
      Proteins matching the same set of peptides:
      SPCC794.09c                       Score: 11     Queries matched: 1
      SPBC839.15c                       Score: 11     Queries matched: 1
14.   SPACUNK4.13c                      Score: 11     Queries matched: 1
 |||GTP binding|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   453.81   905.60   906.50   -0.89 2  11  5.4 10  K.SVGKCKTK.G

15.   SPAC4G8.13c                       Score: 11     Queries matched: 1
 |prz1||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 12   584.86   1167.70   1167.65   0.05 2  11  4.4 2  K.RFTRAYNLK.S

16.   SPCC1259.12c                      Score: 10     Queries matched: 1
 |||SPRY domain|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 12   584.86   1167.70   1166.64   1.05 1  10  4.8 3  K.NGAYLGVAFKK.V

17.   SPBC3B9.14c                       Score: 10     Queries matched: 1
 |mrpl3||mitochondrial ribosomal protein l3|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   567.37   1132.73   1133.58   -0.85 0  12  3.4 2  R.IGLPESYPMK.V

18.   SPAC22E12.02                      Score: 10     Queries matched: 1
 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   403.26   804.50   803.42   1.08 1  10  7.1 3  R.NQTKASR.D

19.   SPBC29A10.04                      Mass: 141659   Score: 10     Queries matched: 1
 |psm1|smc1|mitotic cohesin complex |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 12   584.86   1167.70   1166.56   1.14 1  10  5.5 4  K.EFLKDMQEK.E

20.   SPAC17C9.08                       Score: 10     Queries matched: 1
 |pnu1|nuc1|endodeoxyribonuclease|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 14   614.88   1227.75   1226.69   1.06 0  10  6.3 2  R.ASGLEILSNVPK.G

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   547.37   546.36   546.31   0.05 0  9  4.6 1  ASITR
4   428.31   854.60   856.39   -1.79 0  4  23 1  TYSGNSTK
16   690.91   1379.82   1381.75   -1.93 1  2  31 1  GILAKVSSMPAHR + Oxidation (M)
7   458.84   915.66   916.41   -0.75 1  2  45 1  KHSCEEK
2   362.26   722.51        
8   472.34   942.67        
17   705.39   1408.77        
19   747.38   1492.76        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 2 Da
Fragment Mass Tolerance: ± 0.8 Da
Max Missed Cleavages   : 2
Instrument type        : ESI-QUAD-TOF
Number of queries      : 21

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 21 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  9.3    0.05        M.ASITR.L
  7.8    1.04        K.ASIQK.R
  7.8    0.06        R.ASLEK.Q
  7.8    1.01        R.GTLKK.G
  7.8    1.01        K.SAIKK.Y
  7.8    1.04        R.SAIQK.D
  7.8    0.06        SALEK
  7.8    1.01        K.SALKK.V
  7.8    1.04        K.SALQK.V
  7.8    0.06        R.TGIEK.N
Top scoring peptide matches to query 2
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 3
Score greater than 26 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 40.7    0.09    1  TRBOTR        K.SAASLNSR.V
 11.4    0.08   11  SPAC16A10.07c R.SAARSGTR.R
  9.8    1.08   18  SPAC22E12.02  R.NQTKASR.D
  7.7    0.07                     M.ATSGIQTK.V
  7.4    0.04                     R.TKSWKR.A
  6.2   -0.87                     K.DDSWKR.W
  5.3    0.06                     -.MDLLGLK.E
  3.4    1.09                     K.SDTALAAR.E
  2.3    1.05                     K.KTSAIER.V
  2.2    1.02                     K.TKSSLIR.N
Top scoring peptide matches to query 4
Score greater than 16 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.6   -1.79        R.TYSGNSTK.I
  3.5    0.15        K.TYSPLFK.A
  3.5   -0.85        K.HKSDNKK.T
  3.0    1.12        R.HTLISAGR.I
  1.0    1.24        K.TYHMMR.N
  1.0    1.24        K.TYHMMR.N
  0.5    1.15        R.GEILPPASA.-
  0.0    0.09        K.KHTSVKR.S
Top scoring peptide matches to query 5
Score greater than 22 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 11.7    0.09    2  b1,6glucanase K.FQNHYR.D
  8.6    0.09                     -.MQVVDEK.T
  8.4    1.11                     K.NCEALEK.E
  7.9    0.02                     K.FEIKAEK.C
  7.1    1.01                     R.INTLFQK.D
  6.3    0.18                     R.DEDDDQK.H
  6.1    0.06                     -.MSLQLEK.N
  5.4   -0.81                     K.MENSMTH.-
  5.3    1.04                     K.EEMAKKK.L
  2.3    0.03                     K.STSKKGEK.S
Top scoring peptide matches to query 6
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 27.5    0.10    1  TRBOTR        K.NKPGVYTK.V
 18.7    0.08    7  SPBC13G1.10c  K.ELKELFK.Y
 13.9    0.13    6  SPBC30D10.17c K.EIETTSVK.Q
 12.8   -0.92    3  SPBC83.01     K.AGDPPILPK.Q
 11.9    1.13    9  SPAC23C4.07   K.QIEQMLK.F
 11.5    1.10   10  SPBC3E7.05c   R.LQQELFK.L
 11.0    0.15                     K.ELMEKEK.K
 11.0    0.15   12  SPAC3A12.04c  K.ELYPEQK.I
 11.0    1.06    4  SPBC19C7.07c  K.KIEIMKK.L
 10.8   -0.89   14  SPACUNK4.13c  K.SVGKCKTK.G
Top scoring peptide matches to query 7
Score greater than 14 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.8   -0.75        K.KHSCEEK.S
  1.8    0.20        R.YTSAYRR.N
Top scoring peptide matches to query 8
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 9
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 65.5    0.12    1  TRBOTR K.APILSDSSCK.S
  0.9   -1.85              K.CIDMFIHK.S
Top scoring peptide matches to query 10
Score greater than 26 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 15.5    0.12    2  b1,6glucanase R.FEPALASGITK.I
 12.3   -0.85   17  SPBC3B9.14c   R.IGLPESYPMK.V
  9.0    0.16                     K.IDAEDKVSTR.G
  5.6   -1.80                     R.EMVSWLLEE.-
  5.4    1.14                     R.EKIQTETQR.L
  4.6    1.11                     R.KMGAGDLLRR.Q
  4.3    0.03                     R.YLLAKILSGR.I
  4.0    1.17                     K.NPIDLGTMQK.K
  3.9   -0.80                     R.YNPEEDLVR.I
  3.8    0.16                     R.FNMPAPLATR.F
Top scoring peptide matches to query 11
Score greater than 24 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 59.9    0.13    1  TRBOTR K.SSGTSYPDVLK.C
  7.9   -0.90              -.MDAIHPVSLR.N
  6.1   -1.92              R.GYNIFGLMLK.K
  4.8   -0.93              R.AVYKHMLHR.S
  4.6    0.03              R.LPPNGKITTGR.K
  4.4   -0.83              K.SSQNGDMFLR.C
  4.0   -0.90              -.MLKTFNSSAR.S
  2.2    1.09              K.SMLSSEKRSK.N
  1.6    1.07              K.ATASESFKALK.S
  1.5    0.03              K.KEIITHKER.I
Top scoring peptide matches to query 12
Score greater than 26 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 50.8    0.13    1  TRBOTR       K.VCNYVSWIK.Q
 10.6    0.05   15  SPAC4G8.13c  K.RFTRAYNLK.S
 10.3    1.05   16  SPCC1259.12c K.NGAYLGVAFKK.V
  9.7    1.14   19  SPBC29A10.04 K.EFLKDMQEK.E
  8.2    0.10                    R.GIDHNDTRLK.R
  7.2    0.09                    K.NYSKSTDLLK.K
  7.2    1.03                    R.KNRHMQLIK.K
  6.6   -0.91                    R.IFVCFAERK.S
  6.5    1.19                    R.LSNGCVDMQK.V
  6.3    1.02                    R.FEKVTRFLK.K
Top scoring peptide matches to query 13
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 59.5    0.14    2  b1,6glucanase K.KLHVQFMSSK.W
 14.7    0.11    5  SPBC4C3.09    K.LKHHPETKSK.Y
 11.0    0.10   13  SPAC23A1.10   K.KIEESPKFVK.S
  9.4    0.05                     K.KLNLLSKFNK.Y
  9.3    0.11    3  SPBC83.01     R.KLEEKIESTK.Y
  9.0   -0.84                     R.ELQIWFQNK.R
  8.7    0.01                     R.KIIVKIFTSR.K
  8.4    1.19                     R.QIEQNAAASGSK.D
  8.1   -1.81                     K.EYTAEIQQPK.T
  7.5    0.11                     R.KLGPEYVSRR.S
Top scoring peptide matches to query 14
Score greater than 26 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 55.7    0.14    2  b1,6glucanase R.DAEAALNVPSNK.K
  9.6    1.06   20  SPAC17C9.08   R.ASGLEILSNVPK.G
  2.9   -0.97                     R.APVAKFLADGLK.G
  0.3    0.02                     K.QIFRLIDPVK.T
  0.1    0.20                     K.EAFSYADAQAR.I
Top scoring peptide matches to query 15
Score greater than 19 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 49.4    0.16    2  b1,6glucanase R.DAEAALNVPSNKK.L
  3.4    0.21                     R.EQTVYYAHAFK.N
  3.4   -1.86                     R.IDSVIEEELRR.M
  2.4    0.11                     K.SVAKPQKISENR.I
  1.6   -1.92                     K.LTSNEQISLLLK.I
  1.4    1.15                     K.IPDWVLEQDLK.I
  0.5    1.08                     K.QGIASLLLDNALK.K
Top scoring peptide matches to query 16
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.4   -1.93        K.GILAKVSSMPAHR.Q
  0.5   -1.97        R.VVVFARTTPQHK.M
  0.1   -1.97        K.KIQRESIPFHK.R
Top scoring peptide matches to query 17
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 18
Score greater than 20 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 62.5    0.17    1  TRBOTR K.LQGIVSWGSGCAQK.N
  3.8   -1.82              K.RSPAAAFDEMRNK.L
  1.5   -0.89              R.QIGISTLSSNISSGK.G
  0.1    1.19              K.EFLNDLFTEFSK.F
Top scoring peptide matches to query 19
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 20
Score greater than 23 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 94.8    0.36    1  TRBOTR       R.LGEDNINVVEGNEQFISASK.S
  4.6    0.35    4  SPBC19C7.07c K.KTWLIGGSNDRNINMNGEK.S
  4.0   -1.58                    K.QDLSLSWQDTMSNPQDRK.F
  4.0    0.32                    K.TLLSNNLPHMLFYGSPGTGK.T
  2.8    1.41                    R.EDKDCYTMSLLLDVSSTGK.I
Top scoring peptide matches to query 21
Score greater than 22 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 12.1   -1.81    8  SPBC2G2.11 K.KITDFFSFYSLPSTVIGNPKYK.D
  9.1   -1.62                  R.ATATIMLMGDTCTRGCRFCSVK.T
  9.1   -1.76                  R.DDVQMNADVQFSVTSGLIVEIKK.L
  9.0   -1.75                  K.APEPGYIDKVMLTTTDSDQTLIK.V
  8.6   -1.72                  R.LNRLVADYMMANGYHGAAALLCK.D
  7.4   -0.67                  K.EAPMAPFLEANRRHQGEHASMR.Y
  6.5   -1.61                  K.SFCIPEMQTYRCGICGITSQK.I
  5.5   -1.67                  K.WVEVGNSGMFRPEMLEPMGLPK.D
  5.0   -1.79                  K.ERLTSVVETEHEYYTELVKVK.E
  5.0   -1.75                  -.MEKSQHTLFRPCIDIHQGQVK.Q