Mascot Search Results

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MS data file    : cw0339_q3.pkl
Database        : spombe  (5049 sequences; 2391832 residues)
Timestamp       : 27 Sep 2006 at 10:55:02 GMT
Significant hits: TRBOTR         trypsin (EC3.4.21.4 ) precursor-bovine
                  b1,3glucanase  min sig.pept ACC M38734|O.xanthineolytica 512 aa
                  mSPCC1322.10   |predicted|: Predicted GPI protein

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 30 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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        Error tolerant   

1.    TRBOTR                            Mass: 24662    Score: 496    Queries matched: 7
 trypsin (EC3.4.21.4 ) precursor-bovine
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   453.81   905.61   905.50   0.11 0  19  0.81 1  K.NKPGVYTK.V
6   539.33   1076.64   1076.52   0.12 0  51  0.00043 1  K.APILSDSSCK.S
10   577.38   1152.74   1152.57   0.17 0  59  6.2e-005 1  K.SSGTSYPDVLK.C
11   584.86   1167.71   1167.57   0.14 0  73  2.4e-006 1  K.VCNYVSWIK.Q
15   745.96   1489.91   1489.73   0.17 0  86  1.2e-007 1  K.LQGIVSWGSGCAQK.N
21   1082.16   2162.31   2162.05   0.26 0  137  8.7e-013 1  R.LGEDNINVVEGNEQFISASK.S
22   758.47   2272.38   2272.15   0.22 0  70  4.4e-006 1  K.SIVHPSYNSNTLNNDIMLIK.L

2.    b1,3glucanase                     Mass: 54953    Score: 376    Queries matched: 8
 min sig.pept ACC M38734|O.xanthineolytica 512 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   452.31   902.61   902.50   0.11 0  19  1 1  R.TLCAALVR.T
7   540.83   1079.65   1079.52   0.12 0  94  2e-008 1  R.SGSGQEVAAFK.K
9   554.34   1106.66   1106.53   0.13 1  49  0.00076 1  R.GRVDGDWFR.F
12   604.39   1206.77   1206.64   0.13 0  63  2.6e-005 1  R.ALNPSHGIDVGK.I
13   640.43   1278.84   1278.70   0.14 0  40  0.0054 1  R.GPIYLYVLGER.D
17   778.02   1554.02   1553.84   0.18 0  60  5.2e-005 1  -.VPATIPLTITNDSGR.G
18   801.52   1601.02   1600.83   0.18 0  41  0.0036 1  K.DLQAPNDHVVGPIAR.T
19   924.54   1847.06   1846.87   0.19 0  9  5.1 1  R.DMVVTPFSHEPGTQFR.G

3.    mSPCC1322.10                      Mass: 23944    Score: 30     Queries matched: 1
 |predicted|: Predicted GPI protein
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   423.78   845.55   845.46   0.09 0  30  0.07 1  -.VSVTSPTR.D

4.    SPAC13G6.10c                      Mass: 54406    Score: 28     Queries matched: 1
 |||serine-rich protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
20   1034.13   2066.25   2066.01   0.24 0  28  0.069 1  R.GLAWIPGTDLGYSDNFVNK.G

 
      Proteins matching the same set of peptides:
      mSPAC13G6.10c                     Mass: 52568    Score: 28     Queries matched: 1
 |predicted| probably mild-alkali sensitive
5.    SPCC895.09c                       Mass: 151098   Score: 20     Queries matched: 3
 |||RNA helicase|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   584.86   1167.71   1165.76   1.95 1  9  6.2 3  K.AWPKVVILLK.M
 18   801.52   1601.02   1600.70   0.32 1  10  4.8 2  K.LNEDDDEEDVPRR.K
 19   924.54   1847.06   1845.92   1.14 1  1  39 7  R.TFPIKDYYIEDFAPK.L

6.    SPCC737.04                        Score: 15     Queries matched: 1
 |B8647-5||Pfam-B_8647 domain|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   453.81   905.61   906.52   -0.91 1  15  2.2 2  R.QDKLYLK.S

7.    SPACUNK4.13c                      Score: 14     Queries matched: 1
 |||GTP binding|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   453.81   905.61   906.50   -0.89 2  14  2.7 3  K.SVGKCKTK.G

8.    SPBC32H8.13c                      Score: 13     Queries matched: 2
 |mok12||alpha-1,3-glucan synthase|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   423.78   845.55   847.43   -1.87 0  13  3.7 2  K.TVSTPSEK.L
 9   554.34   1106.66   1106.38   0.27 0  2  38 5  K.TYDMDCMR.Q + Oxidation (M)

9.    SPAC16C9.07                       Score: 13     Queries matched: 1
 ||SPAC2G11.01|serine/threonine protein kinase |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   452.31   902.61   902.53   0.08 2  13  3.8 2  R.TLGKNSKR.A

10.   SPAC4G8.13c                       Score: 12     Queries matched: 1
 |prz1||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   584.86   1167.71   1167.65   0.06 2  12  3 2  K.RFTRAYNLK.S

11.   SPBC25H2.11c                      Score: 12     Queries matched: 1
 |||bromodomain protein|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   577.38   1152.74   1152.66   0.07 1  12  3.1 2  R.LPPNGKITTGR.K

12.   SPBC3E7.05c                       Mass: 63461    Score: 12     Queries matched: 1
 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   453.81   905.61   904.50   1.10 0  12  3.9 4  R.LQQELFK.L

13.   SPBC13E7.03c                      Score: 12     Queries matched: 1
 |||SAM domain |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   403.27   804.52   803.43   1.08 0  12  4.3 1  R.GIMALGAR.R + Oxidation (M)

14.   SPBC18H10.11c                     Score: 12     Queries matched: 1
 |||hypothetical protein|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   403.27   804.52   803.49   1.03 1  12  4.3 1  K.TKSSLIR.N

15.   SPAC26A3.09c                      Score: 12     Queries matched: 2
 |rga2||GTPase activating protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   403.27   804.52   803.45   1.07 1  12  4.3 1  K.TTDIKAR.S
 2   423.78   845.55   845.48   0.07 1  1  64 7  M.VKMDVVR.T

16.   SPAC110.01                        Score: 12     Queries matched: 1
 ||SPAC140.05|serine/threonine protein kinase |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   403.27   804.52   803.45   1.07 1  12  4.3 1  K.KTSAIER.V

17.   SPCC970.08                        Score: 12     Queries matched: 1
 |||inositol polyphosphate kinase |Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   403.27   804.52   803.40   1.12 0  12  4.3 1  K.GMQIAER.G

18.   SPAC1782.03                       Mass: 40716    Score: 12     Queries matched: 1
 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   403.27   804.52   803.39   1.13 1  12  4.3 1  K.DAEASRR.G

19.   SPAC1834.06c                      Mass: 38782    Score: 12     Queries matched: 1
 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   403.27   804.52   803.41   1.10 0  12  4.3 1  K.STQDVVR.C

20.   SPAPB18E9.01                      Score: 12     Queries matched: 1
 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   403.27   804.52   806.37   -1.85 0  12  4.7 8  K.AFNQDGR.E

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
25   1459.69   2917.36   2915.43   1.93 1  10  3 1  APSMGAKSLCIPLEQPSGEDAIIEGTTK + Oxidation (M)
23   1137.21   2272.41   2271.11   1.30 1  6  9.9 1  QMADTCYQEILVLKSSIEK + Oxidation (M)
14   690.93   1379.85   1381.75   -1.90 1  6  13 1  GILAKVSSMPAHR + Oxidation (M)
16   747.40   1492.78   1493.93   -1.15 2  3  25 1  TPGLKKLAEVILGR
24   1275.78   2549.55   2551.27   -1.72 1  3  20 1  APEPGYIDKVMLTTTDSDQTLIK + Oxidation (M)
5   529.83   1057.64   1058.51   -0.87 1  3  35 1  RVPAWCDR
8   548.81   1095.61        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 2 Da
Fragment Mass Tolerance: ± 0.8 Da
Max Missed Cleavages   : 2
Instrument type        : ESI-QUAD-TOF
Number of queries      : 25

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 20 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 11.9    1.13   18  SPAC1782.03   K.DAEASRR.G
 11.9    1.08   13  SPBC13E7.03c  R.GIMALGAR.R
 11.9    1.12   17  SPCC970.08    K.GMQIAER.G
 11.9    1.07   16  SPAC110.01    K.KTSAIER.V
 11.9    1.10   19  SPAC1834.06c  K.STQDVVR.C
 11.9    1.03   14  SPBC18H10.11c K.TKSSLIR.N
 11.9    1.07   15  SPAC26A3.09c  K.TTDIKAR.S
 11.6   -1.85   20  SPAPB18E9.01  K.AFNQDGR.E
  6.8   -1.91                     R.LSDIFGR.R
  6.2   -1.86                     R.ASAHHER.R
Top scoring peptide matches to query 2
Score greater than 27 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 30.4    0.09    3  mSPCC1322.10 -.VSVTSPTR.D
 13.1   -1.87    8  SPBC32H8.13c K.TVSTPSEK.L
 11.7    0.13                    K.VSWTPEK.F
  1.4    0.11                    R.TIAAPSMR.F
  1.4    0.09                    R.TIAEREK.L
  1.1   -1.90                    K.TVSILCR.G
  0.8   -2.00                    R.TVKFIIK.L
  0.8    0.07   15  SPAC26A3.09c M.VKMDVVR.T
  0.6   -1.89                    K.LMQGMLR.K
  0.3    0.06                    R.VSNKLTGK.D
Top scoring peptide matches to query 3
Score greater than 26 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 18.6    0.11    2  b1,3glucanase R.TLCAALVR.T
 12.8    0.08    9  SPAC16C9.07   R.TLGKNSKR.A
  9.8    0.08                     K.TIERTKR.K
  7.0   -0.92                     K.KSLWSKR.F
  4.7    0.12                     K.LTEAREGK.L
  3.7    0.10                     K.KSTEPLTK.R
  3.2   -0.83                     R.SENSPSKR.K
  3.0   -1.84                     R.GVDIMSQR.L
  2.4   -0.81                     R.TNGQQSGGR.F
  1.9    0.06                     K.KSSILDIK.K
Top scoring peptide matches to query 4
Score greater than 28 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 19.1    0.11    1  TRBOTR       K.NKPGVYTK.V
 14.7   -0.91    6  SPCC737.04   R.QDKLYLK.S
 13.9   -0.89    7  SPACUNK4.13c K.SVGKCKTK.G
 12.2    1.10   12  SPBC3E7.05c  R.LQQELFK.L
  8.6    0.13                    K.LESLTSEK.S
  7.9    0.13                    K.EIETTSVK.Q
  7.8    0.08                    K.ELKELFK.Y
  7.2    1.09                    K.IQQYVVR.L
  7.2    1.10                    K.IQLFEQK.R
  6.8    0.12                    K.QLKDTSSK.L
Top scoring peptide matches to query 5
Score greater than 14 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.5   -0.87        K.RVPAWCDR.I
  1.6   -1.94        K.LLERCLEK.N
  1.6    0.12        K.QPKMHDFR.Q
Top scoring peptide matches to query 6
Score greater than 18 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 51.4    0.12    1  TRBOTR K.APILSDSSCK.S
  2.0   -1.86              K.CIDMFIHK.S
Top scoring peptide matches to query 7
Score greater than 23 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 94.4    0.12    2  b1,3glucanase R.SGSGQEVAAFK.K
  5.0   -1.93                     R.VIEPGSPLDR.E
  3.8   -0.78                     R.CAEWSTDGR.W
  1.9    1.20                     K.SRDGEDEGSK.E
Top scoring peptide matches to query 8
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 9
Score greater than 22 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 48.8    0.13    2  b1,3glucanase R.GRVDGDWFR.F
  6.3   -1.89                     R.SQYEDLAKR.N
  3.5    0.13                     K.HNNFSSFVR.Q
  3.2   -0.02                     K.GIHISLNLIK.W
  1.8    0.27    8  SPBC32H8.13c  K.TYDMDCMR.Q
  1.7    0.03                     R.RRYSILGSR.K
  0.8    0.03                     K.RAFPKTPYK.I
  0.7   -1.89                     K.LGFQRDSMR.N
  0.6    0.06                     K.YTNELLLNK.E
  0.3    0.12                     K.SSNKEGRSSR.G
Top scoring peptide matches to query 10
Score greater than 29 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 59.3    0.17    1  TRBOTR       K.SSGTSYPDVLK.C
 12.4    0.07   11  SPBC25H2.11c R.LPPNGKITTGR.K
  8.8    1.14                    K.SMLSSEKRSK.N
  6.1   -0.78                    K.SSQNGDMFLR.C
  5.8    1.19                    K.DDDFIQGSKK.D
  4.5    0.14                    R.QKVSEPYFR.G
  4.3    1.19                    K.QTFQTPSSEK.S
  4.3    1.22                    K.VMDMIDGVEK.K
  4.2    1.17                    M.QGSIYEGSRR.N
  3.0    0.06                    K.KASFIYRLR.K
Top scoring peptide matches to query 11
Score greater than 29 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein     Peptide
 73.3    0.14    1  TRBOTR      K.VCNYVSWIK.Q
 12.4    0.06   10  SPAC4G8.13c K.RFTRAYNLK.S
  9.3    1.95    5  SPCC895.09c K.AWPKVVILLK.M
  8.4    0.11                   R.VYKESQRMK.V
  8.1    0.04                   K.VPVLCVSPGLK.H
  8.0   -0.84                   R.EMFEDSRKK.T
  6.2    0.16                   R.VLMYEEEQK.L
  5.8    1.04                   R.KNRHMQLIK.K
  5.8    0.10                   K.NYSKSTDLLK.K
  5.8   -1.91                   K.EMLHTLWLK.L
Top scoring peptide matches to query 12
Score greater than 24 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 63.4    0.13    2  b1,3glucanase R.ALNPSHGIDVGK.I
  7.7   -0.81                     R.ANIEEDYAKR.M
  7.6   -0.86                     R.KGLAEGMVFEK.E
  5.0    0.12                     R.AGYTVQERRK.I
  2.1   -1.81                     K.KQNPEFFDGK.N
  1.1   -0.87                     K.MFLGVRADVGK.Y
  0.4    0.17                     K.TERDTSTNRK.Q
Top scoring peptide matches to query 13
Score greater than 18 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 39.9    0.14    2  b1,3glucanase R.GPIYLYVLGER.D
  2.8   -1.81                     R.FKRLYDMHR.H
Top scoring peptide matches to query 14
Score greater than 19 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  6.1   -1.90        K.GILAKVSSMPAHR.Q
Top scoring peptide matches to query 15
Score greater than 22 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 86.3    0.17    1  TRBOTR K.LQGIVSWGSGCAQK.N
  3.7   -0.88              R.QIGISTLSSNISSGK.G
  2.3   -0.93              R.KLGSGSFGVVWKAR.E
  1.5    0.06              K.IVTDPAKLVPYFK.N
  0.9    0.12              K.QVVPSSQRLADYK.C
Top scoring peptide matches to query 16
Score greater than 16 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.3   -1.15        R.TPGLKKLAEVILGR.D
Top scoring peptide matches to query 17
Score greater than 20 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 60.2    0.18    2  b1,3glucanase -.VPATIPLTITNDSGR.G
  3.5   -1.83                     R.VGVILMSRQAETPR.K
  1.9    0.08                     K.RPDLIVPYKARVK.A
  1.6    0.19                     K.TLGPIANAINACALR.T
  1.5    0.17                     R.VYMAEFLELLAKK.V
  1.1    0.20                     R.SGSIHSDIRVSQLR.E
  0.6   -1.83                     K.LTVGLNSVNELLER.C
Top scoring peptide matches to query 18
Score greater than 25 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 41.4    0.18    2  b1,3glucanase K.DLQAPNDHVVGPIAR.T
 10.2    0.32    5  SPCC895.09c   K.LNEDDDEEDVPRR.K
  8.1    0.17                     R.DERKPQFPFKGPR.S
  6.4    1.18                     R.LNKFSYPNYVLSR.T
  4.2    1.13                     R.ISILEKEKEDLQR.K
  3.2    0.15                     R.NVDVWQRMLRIR.S
  2.4    1.14                     R.LIKSANPEAFGLNAR.G
  1.3    0.28                     K.GELQTAMEIMDHAR.K
  1.2   -0.84                     R.DLIGLLRAYSYYR.F
  0.6   -1.89                     K.KSLTYSSFLAFLVK.L
Top scoring peptide matches to query 19
Score greater than 20 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
  9.4    0.19    2  b1,3glucanase R.DMVVTPFSHEPGTQFR.G
  7.0    1.10                     R.SKTLDNAALDLPYVGNR.K
  4.8   -1.67                     K.MDVMCQSMSFNADGTR.L
  2.9   -1.89                     K.DRRPKYMENLIESAK.K
  2.2   -0.79                     K.DDDTVLEDNQLKSNSR.I
  1.3   -1.97                     R.DIKPANILLDHRGMIK.Y
  0.6    1.14    5  SPCC895.09c   R.TFPIKDYYIEDFAPK.L
  0.5   -0.85                     K.NYLVRNSSSHPYHFK.K
  0.3   -1.78                     R.MTLPSTDWHSMELSAK.V
  0.3   -1.88                     K.SDCSKNTQLKPSFLPK.N
Top scoring peptide matches to query 20
Score greater than 22 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 27.7    0.24    4  SPAC13G6.10c R.GLAWIPGTDLGYSDNFVNK.G
  7.5   -0.86                    R.ADLEIQRAAIPIMVAQNSK.L
  4.8   -0.86                    K.DITVNAHPNQPSRPRLPR.V
  4.3   -0.80                    R.VSQILERVRDGAHSMPEK.Q
  4.2    0.12                    R.IIAVSGMSDLKWELHVIR.D
  3.6    0.28                    R.SDMYTIKSRWEIPHCK.R
  3.0   -1.88                    R.KLGVKPPKGCLMFGPPGTGK.T
  2.4   -1.76                    R.RAEWDEYLKWAIDAFR.L
  2.3   -0.86                    K.DIHVTLIEAAPGVLPMFTK.S
  2.0   -1.72                    K.NYESEGRLTPPPVSMGYR.Y
Top scoring peptide matches to query 21
Score greater than 27 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
137.1    0.26    1  TRBOTR R.LGEDNINVVEGNEQFISASK.S
  6.4    0.23              K.TLLSNNLPHMLFYGSPGTGK.T
  4.8   -0.82              K.VNALPEETTLAVNHRVDASK.G
  2.8    0.26              K.KTWLIGGSNDRNINMNGEK.S
  1.9   -1.65              R.FISADSLSTCMTDSQLSASK.L
  1.4   -0.67              K.WYTRYANGSPICSEMGKK.E
  0.9   -0.81              K.KVFNNSDEFQPSPLTLLSK.A
  0.5    1.27              K.KDMIFFDIAQSEGFQIEK.I
  0.4    1.16              K.SQENAPQVLLGISQSHVQVK.K
  0.1    0.12              K.VIKCPLSGLQLKLYQQMK.K
Top scoring peptide matches to query 22
Score greater than 25 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 69.8    0.22    1  TRBOTR K.SIVHPSYNSNTLNNDIMLIK.L
  8.0   -0.84              R.GAAQALVCENLRTIQFEILK.Y
  6.6   -0.96              K.KLITQILYLQTLVSEQQKK.I
  6.2   -0.83              K.HSEFISYPIQLVVTREVEK.E
  4.0   -0.89              K.QKVSVPKGAYLLGVADETGTLK.G
  2.3    0.23              -.MEVQKGLIEAFYNTYPLEK.A
  1.2   -0.87              K.KPEPYKGKGIYVDGEKPQLK.A
  0.8    1.27              K.TVLDVGCGTGILSMFCAKAGAK.K
  0.4   -0.85              R.EVGDLSNALSVSMVRLVDILK.F
  0.1   -1.81              R.EALRTATASGDQRMLMPAVLK.D
Top scoring peptide matches to query 23
Score greater than 19 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  6.3    1.30        R.QMADTCYQEILVLKSSIEK.E
Top scoring peptide matches to query 24
Score greater than 15 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.7   -1.72        K.APEPGYIDKVMLTTTDSDQTLIK.V
Top scoring peptide matches to query 25
Score greater than 21 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 10.4    1.93        K.APSMGAKSLCIPLEQPSGEDAIIEGTTK.C
  8.0    0.82        K.IVASMPSKMAVVLQMPRGNLETIYPR.I
  8.0    1.99        R.RDLYTMTDRSPAEHGALDLHMGTSNK.Q