Mascot Search Results

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MS data file    : cw0338_q8.pkl
Database        : spombe  (5049 sequences; 2391832 residues)
Timestamp       : 26 Sep 2006 at 16:59:45 GMT
Significant hits: mSPAC19B12.02C  |predicted|: Predicted GPI protein : SIM. TO S. CEREVISIAE GAS1
                  SPAC13G6.10c    |||serine-rich protein|Schizosaccharomyces pombe|chr 1|||Manual
                  SPAC11E3.13c    |||GPI anchored protein |Schizosaccharomyces pombe|chr 1|||Manual
                  mPSU1           |predicted|: Predicted GPI protein : CELL WALL SYNTHESIS PROTEIN

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 29 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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1.    mSPAC19B12.02C                    Mass: 57007    Score: 90     Queries matched: 2
 |predicted|: Predicted GPI protein : SIM. TO S. CEREVISIAE GAS1
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   526.84   1051.68   1051.54   0.13 0  41  0.0035 1  -.SVSPVHVDGR.Y
9   826.02   1650.03   1649.84   0.19 0  49  0.00047 1  R.DVPYFQELSVNAIR.V

2.    SPAC13G6.10c                      Mass: 54406    Score: 46     Queries matched: 1
 |||serine-rich protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
10   1034.13   2066.24   2066.01   0.23 0  46  0.00074 1  R.GLAWIPGTDLGYSDNFVNK.G

 
      Proteins matching the same set of peptides:
      mSPAC13G6.10c                     Mass: 52568    Score: 46     Queries matched: 1
 |predicted| probably mild-alkali sensitive
3.    SPAC11E3.13c                      Mass: 54058    Score: 41     Queries matched: 2
 |||GPI anchored protein |Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   815.55   1629.08   1628.89   0.19 0  6  10 2  R.DLPYLQGLNINTIR.V
13   1190.78   2379.54   2379.13   0.41 0  35  0.0098 1  R.GVDYQPGGSSTLVDPLADTSICK.R

 
      Proteins matching the same set of peptides:
      mSPAC11E3.13C                     Mass: 51689    Score: 41     Queries matched: 2
 |predicted|: Predicted GPI protein : Gas1 homology
4.    mPSU1                             Mass: 40375    Score: 36     Queries matched: 1
 |predicted|: Predicted GPI protein : CELL WALL SYNTHESIS PROTEIN
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   795.46   1588.90   1588.71   0.19 0  36  0.01 1  K.TQWPSTQPSDGETR.G

 
      Proteins matching the same set of peptides:
      SPAC1002.13c                      Mass: 42295    Score: 36     Queries matched: 1
 |psu1||beta-glucosidase |Schizosaccharomyces pombe|chr 1|||Manual
5.    SPAC823.04                        Mass: 22775    Score: 23     Queries matched: 2
 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   486.85   971.68   971.54   0.14 0  15  1.7 2  R.VSEIQQLR.D
4   523.35   1044.68   1044.59   0.09 1  7  12 1  R.AESIRQTLK.S

6.    SPAC1705.03c                      Mass: 43547    Score: 17     Queries matched: 1
 ||SPAC23H4.19|involved in cell wall biosynthesis |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
14   1284.30   2566.58   2566.26   0.32 0  17  0.51 1  R.YIQEITMEGLESAQNIQISANSK.G

 
      Proteins matching the same set of peptides:
      mSPAC1705.03C                     Mass: 41468    Score: 17     Queries matched: 1
 |predicted|: Predicted GPI protein : Ecm33 family
7.    SPCC10H11.01                      Mass: 114599   Score: 15     Queries matched: 1
 |prp11||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   486.85   971.68   971.58   0.11 1  15  1.7 1  R.ASLDGIKIR.G

8.    SPCC285.11                        Mass: 48869    Score: 15     Queries matched: 1
 |||UBX domain|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   486.85   971.68   972.49   -0.81 0  15  1.7 2  K.QEAIDELR.N

9.    SPBCPT2R1.08c                     Score: 14     Queries matched: 2
 |||helicase|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   435.82   869.64   869.54   0.09 1  5  16 9  K.VVRDIIR.T
 3   486.85   971.68   973.55   -1.87 2  13  3.1 4  R.ECKLRIR.F

 
      Proteins matching the same set of peptides:
      SPAC212.11                        Score: 14     Queries matched: 2
10.   SPBC16E9.10c                      Score: 12     Queries matched: 1
 |||AAA family ATPase|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   526.84   1051.68   1052.40   -0.73 0  12  2.7 2  R.WSSASTNDAD.-

11.   SPAC24C9.08                       Mass: 66850    Score: 11     Queries matched: 1
 |||gly x carboxypeptidase activity |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   1035.14   2068.26   2068.01   0.25 2  11  2.8 1  K.QEKEQETDLEAQVEHKK.R

12.   SPBC839.13c                       Mass: 22327    Score: 8      Queries matched: 1
 |rpl1601||60S ribosomal protein L13/L16|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   435.82   869.64   871.44   -1.80 0  8  7.3 1  K.EASPVNQK.L

13.   SPAC32A11.04c                     Score: 7      Queries matched: 1
 |tif212|tif22, SPAC6B12.17c|translation initiation factor|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   435.82   869.64   870.49   -0.86 0  7  9.4 3  K.QIENVLR.R

14.   SPAC1834.10c                      Score: 7      Queries matched: 1
 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   435.82   869.64   870.57   -0.93 2  7  9.6 4  K.KAIGNIKK.F

15.   SPAC11D3.07c                      Mass: 70569    Score: 6      Queries matched: 1
 |||involved in transcriptional regulation|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   815.55   1629.08   1628.76   0.32 0  6  9.2 1  K.NIEKPEDGEESVQR.R

16.   SPAC806.03c                       Score: 6      Queries matched: 1
 |rps2601|rps26-1, rps26|40S ribosomal protein S26|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   435.82   869.64   870.55   -0.92 2  6  12 6  R.VVRVRSR.E

 
      Proteins matching the same set of peptides:
      SPAC1805.11c                      Mass: 14082    Score: 6      Queries matched: 1
 |rps2602|rps26-2|40S ribosomal protein S26|Schizosaccharomyces pombe|chr 1|||Manual
17.   SPAC1805.17                       Score: 6      Queries matched: 1
 |crm1|caf2, SPAC1B2.01|chromosome region maintenance protein 1|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   421.81   841.60   843.52   -1.91 0  6  13 2  K.ATLGTLLR.F

18.   SPAC6G9.01c                       Score: 6      Queries matched: 1
 |||hypothetical protein|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   435.82   869.64   870.46   -0.82 1  6  13 7  K.KANPANEK.S

19.   SPBC577.07                        Score: 6      Queries matched: 1
 |ubp10||ubiquitin C-terminal hydrolase activity|Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   1035.14   2068.26   2069.12   -0.86 1  6  8.8 2  K.NFDNCPQLVQRLAILIR.K

20.   SPAC14C4.05c                      Mass: 95533    Score: 6      Queries matched: 1
 |||hypothetical protein|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
12   1111.63   2221.25   2221.10   0.15 1  6  8.4 1  K.VEEMVQAFMSIIGKWYYK.A

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   421.81   841.60   841.50   0.11 1  8  9.6 1  GMRLLPR
6   568.33   1134.65        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 2 Da
Fragment Mass Tolerance: ± 0.8 Da
Max Missed Cleavages   : 2
Instrument type        : ESI-QUAD-TOF
Number of queries      : 14

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 19 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein     Peptide
  7.5    0.11                   K.GMRLLPR.L
  6.2   -1.91   17  SPAC1805.17 K.ATLGTLLR.F
  3.0    0.07                   K.ISALGLLR.L
  1.6    0.18                   K.VTAEYFL.-
  0.8   -0.81                   K.LEEEAPR.E
  0.5   -1.85                   K.GDRDLLR.N
  0.2    0.10                   K.EIQALIR.Q
  0.2    0.10                   R.QLEAIIR.I
Top scoring peptide matches to query 2
Score greater than 20 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
  8.4   -1.80   12  SPBC839.13c   K.EASPVNQK.L
  7.5   -0.90                     R.KAGKAVAAR.M
  7.3   -0.86   13  SPAC32A11.04c K.QIENVLR.R
  7.2   -0.93   14  SPAC1834.10c  K.KAIGNIKK.F
  7.1   -0.88                     K.QANKVRR.Q
  6.2   -0.92   16  SPAC806.03c   R.VVRVRSR.E
  5.7   -0.82   18  SPAC6G9.01c   K.KANPANEK.S
  5.7   -0.93                     K.KAWILLK.E
  5.0    0.09    9  SPBCPT2R1.08c K.VVRDIIR.T
  5.0    0.12                     R.VVRNNLR.V
Top scoring peptide matches to query 3
Score greater than 25 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein       Peptide
 15.5    0.11    7  SPCC10H11.01  R.ASLDGIKIR.G
 15.4   -0.81    8  SPCC285.11    K.QEAIDELR.N
 15.4    0.14    5  SPAC823.04    R.VSEIQQLR.D
 12.8   -1.87    9  SPBCPT2R1.08c R.ECKLRIR.F
Top scoring peptide matches to query 4
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
  7.1    0.09    5  SPAC823.04 R.AESIRQTLK.S
  4.0    0.04                  R.LLEVFRLR.S
  3.5    0.12                  -.MSSSPPALKK.F
  2.8    0.14                  R.IASLEDELR.A
  2.5    0.04                  K.LFRDLLIR.K
  2.5    0.09                  K.LPYGVDLLR.L
  2.1    0.06                  K.KFTPRQLR.L
  0.9   -1.82                  M.SDSPIELMR.S
  0.8   -1.86                  K.DITFLADPR.G
  0.8   -1.85                  R.LRMKDNDR.K
Top scoring peptide matches to query 5
Score greater than 27 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein        Peptide
 40.6    0.13    1  mSPAC19B12.02C -.SVSPVHVDGR.Y
 11.8   -0.73   10  SPBC16E9.10c   R.WSSASTNDAD.-
  4.2    0.12                      M.SETTKSGSKK.S
  2.3    0.21                      K.EGSAWYDPK.N
  1.2    0.15                      R.EGYLNSNKK.Q
  1.1    0.15                      K.GEYIDATKR.G
  0.8    0.16                      K.QCTLEFVR.Q
Top scoring peptide matches to query 6
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 7
Score greater than 16 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 35.8    0.19    4  mPSU1 K.TQWPSTQPSDGETR.G
  0.9   -1.88             K.DSSKPTEDQESKLK.V
  0.7   -1.93             K.STALSLTSSPLNSWK.T
Top scoring peptide matches to query 8
Score greater than 19 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  6.4    0.32   15  SPAC11D3.07c K.NIEKPEDGEESVQR.R
  5.9    0.19    3  SPAC11E3.13c R.DLPYLQGLNINTIR.V
  4.5   -0.76                    -.MPSLNQEETPKKTK.N
  2.4   -0.76                    R.EVDLGIISTDVDNLK.N
  2.0    1.19                    R.LNNNEKLEATQVKK.I
  1.9   -0.76                    K.IDSISIEELDLIDR.I
  0.9    0.29                    K.MPEGSPPTSRSIQSR.N
  0.6    1.19                    R.RAGPNYQEGLRVIR.E
Top scoring peptide matches to query 9
Score greater than 18 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein        Peptide
 49.0    0.19    1  mSPAC19B12.02C R.DVPYFQELSVNAIR.V
  2.8    1.11                      R.SALRSGFLPSLTSSVK.T
Top scoring peptide matches to query 10
Score greater than 19 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 46.2    0.23    2  SPAC13G6.10c R.GLAWIPGTDLGYSDNFVNK.G
  3.7   -0.87                    K.KLDAINRLSNIIYYSER.V
  2.2   -0.87                    K.DITVNAHPNQPSRPRLPR.V
  2.1    0.22                    R.GWLAEGGTLIGTARCMAFR.E
  1.7    0.06                    R.LALSRFLEKPIRYFTGR.T
  1.1   -1.77                    R.RAEWDEYLKWAIDAFR.L
  1.1    1.16                    K.AREAAELARAASAEIPFHR.L
  1.1   -1.92                    R.TLQSLSCARIRPLVMVPK.S
  0.7    1.16                    K.GNSLEVRRELGNCLAHLK.K
  0.6   -1.77                    R.NGSRQTVFFSATWPESVR.A
Top scoring peptide matches to query 11
Score greater than 19 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein     Peptide
 10.6    0.25   11  SPAC24C9.08 K.QEKEQETDLEAQVEHKK.R
  5.6   -0.86   19  SPBC577.07  K.NFDNCPQLVQRLAILIR.K
  4.7    1.15                   K.KLDAINRLSNIIYYSER.V
  4.2    1.23                   K.TEFYPGEVCLLKCRPAK.R
  4.1   -1.69                   K.EQQASQSPSVEGENEPAGVK.S
  3.4    0.07                   R.NIVTSDIQNEKSLLLLIR.D
  2.7   -1.77                   K.APDGSLSRAAMQGQILANDR.R
  2.7   -0.88                   K.KVSDIALEKQLLEEAVER.Y
  2.4   -1.81                   K.KDALHTLLPGFMRCFHK.V
  2.4   -0.84                   R.KNDNVLGLTEDEIQQILK.T
Top scoring peptide matches to query 12
Score greater than 15 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  5.5    0.15   20  SPAC14C4.05c K.VEEMVQAFMSIIGKWYYK.A
  2.7    0.28                    R.ESHSTPHDYEPHVSDKNDK.S
  2.2   -0.91                    R.TMVAENFGDVGIGKVASSLTVK.Y
  1.6    1.24                    K.NEEEKEFDLGLEENPDSVK.K
  0.7    1.28                    K.DICPDEFNEWKDPVEAEK.D
Top scoring peptide matches to query 13
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 34.7    0.41    3  SPAC11E3.13c R.GVDYQPGGSSTLVDPLADTSICK.R
Top scoring peptide matches to query 14
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 17.0    0.32    6  SPAC1705.03c R.YIQEITMEGLESAQNIQISANSK.G