Mascot Search Results

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MS data file    : cw0338_q7.pkl
Database        : spombe  (5049 sequences; 2391832 residues)
Timestamp       : 26 Sep 2006 at 15:15:59 GMT
Significant hits: SPAC1705.03c  ||SPAC23H4.19|involved in cell wall biosynthesis |Schizosaccharomyces pombe|chr 1|||Manual
                  SPAC11E3.13c  |||GPI anchored protein |Schizosaccharomyces pombe|chr 1|||Manual
                  mPSU1         |predicted|: Predicted GPI protein : CELL WALL SYNTHESIS PROTEIN
                  SPBC32F12.11  |tdh1|gpd1|glyceraldehyde 3-phosphate dehydrogenase|Schizosaccharomyces pombe|chr 2|||Manual
                  mSPCC1322.10  |predicted|: Predicted GPI protein
                  SPAC23A1.10   |ef1a-b||translation elongation factor |Schizosaccharomyces pombe|chr 1|||Manual

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 29 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    SPAC1705.03c                      Mass: 43547    Score: 87     Queries matched: 2
 ||SPAC23H4.19|involved in cell wall biosynthesis |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   409.78   817.55   817.45   0.09 0  47  0.0015 1  K.GALTVETK.A
4   419.27   836.53   836.44   0.09 0  41  0.0037 1  K.GVSVNFSK.L

 
      Proteins matching the same set of peptides:
      mSPAC1705.03C                     Mass: 41468    Score: 87     Queries matched: 2
 |predicted|: Predicted GPI protein : Ecm33 family

2.    SPAC11E3.13c                      Mass: 54058    Score: 51     Queries matched: 2
 |||GPI anchored protein |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
16   686.38   1370.74   1370.59   0.16 0  14  1.7 1  K.GNAFFNSDTNER.F
21   1190.70   2379.39   2379.13   0.27 0  36  0.0067 1  R.GVDYQPGGSSTLVDPLADTSICK.R

 
      Proteins matching the same set of peptides:
      mSPAC11E3.13C                     Mass: 51689    Score: 51     Queries matched: 2
 |predicted|: Predicted GPI protein : Gas1 homology
3.    mPSU1                             Mass: 40375    Score: 49     Queries matched: 2
 |predicted|: Predicted GPI protein : CELL WALL SYNTHESIS PROTEIN
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
15   565.85   1129.68   1129.54   0.14 0  25  0.16 1  K.GTSAQYYVNK.A
18   795.46   1588.90   1588.71   0.19 0  23  0.18 1  K.TQWPSTQPSDGETR.G

 
      Proteins matching the same set of peptides:
      SPAC1002.13c                      Mass: 42295    Score: 49     Queries matched: 2
 |psu1||beta-glucosidase |Schizosaccharomyces pombe|chr 1|||Manual
4.    SPBC32F12.11                      Mass: 36018    Score: 47     Queries matched: 1
 |tdh1|gpd1|glyceraldehyde 3-phosphate dehydrogenase|Schizosaccharomyces pombe|chr 2|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
17   734.99   1467.96   1467.82   0.14 0  47  0.00088 1  R.VPTPDVSVVDLTVK.L

 
      Proteins matching the same set of peptides:
      SPBC354.12                        Mass: 35823    Score: 47     Queries matched: 1
 |gpd3||glyceraldehyde 3-phosphate dehydrogenase|Schizosaccharomyces pombe|chr 2|||Manual
5.    mSPCC1322.10                      Mass: 23944    Score: 33     Queries matched: 1
 |predicted|: Predicted GPI protein
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   423.78   845.55   845.46   0.09 0  33  0.037 1  -.VSVTSPTR.D

6.    SPAC23A1.10                       Mass: 50099    Score: 31     Queries matched: 1
 |ef1a-b||translation elongation factor |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   488.33   974.65   974.54   0.11 0  31  0.05 1  R.LPLQDVYK.I

 
      Proteins matching the same set of peptides:
      SPCC794.09c                       Mass: 50085    Score: 31     Queries matched: 1
 |ef1a-a||translation elongation factor 1 |Schizosaccharomyces pombe|chr 3|||Manual
      SPBC839.15c                       Mass: 50099    Score: 31     Queries matched: 1
 |ef1a-c||translation elongation factor |Schizosaccharomyces pombe|chr 2|||Manual
7.    SPAPB2B4.03                       Mass: 47732    Score: 16     Queries matched: 1
 |cig2|cyc17|cyclin|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   419.27   836.53   836.42   0.12 1  16  1.1 2  R.RSCSLSK.F

8.    SPBC14C8.14c                      Score: 16     Queries matched: 1
 |pol5||DNA polymerase V|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   409.78   817.55   817.43   0.12 1  16  1.8 3  K.KNAQETK.Q

9.    SPBC3D6.12                        Score: 14     Queries matched: 1
 |||WD repeat protein|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   409.78   817.55   817.45   0.09 0  17  1.5 2  K.QTIESLK.D

10.   SPBC32H8.13c                      Score: 12     Queries matched: 1
 |mok12||alpha-1,3-glucan synthase|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   423.78   845.55   847.43   -1.88 0  12  4.1 3  K.TVSTPSEK.L

11.   SPBC354.08c                       Score: 12     Queries matched: 1
 |||DUF221|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   419.27   836.53   838.44   -1.91 1  12  3.1 4  K.KHDNAVR.S

12.   SPAC13G6.10c                      Mass: 54406    Score: 10     Queries matched: 1
 |||serine-rich protein|Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
19   1034.13   2066.24   2066.01   0.23 0  10  3.1 1  R.GLAWIPGTDLGYSDNFVNK.G

 
      Proteins matching the same set of peptides:
      mSPAC13G6.10c                     Mass: 52568    Score: 10     Queries matched: 1
 |predicted| probably mild-alkali sensitive
13.   SPAC23G3.12c                      Score: 10     Queries matched: 1
 |||PDZ domain protein|Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   409.78   817.55   817.47   0.08 0  10  7 5  R.QYALPVK.G

14.   SPAC1952.08c                      Score: 10     Queries matched: 1
 |||pyridoxamine 5'-phosphate oxidase |Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   419.27   836.53   836.42   0.12 1  10  4.8 5  K.RCLSSSK.Y

15.   SPBC776.08c                       Score: 10     Queries matched: 1
 |||Nrap|Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   409.78   817.55   817.47   0.08 0  10  7.5 6  R.QIFPVSK.L

16.   SPCC162.06c                       Score: 9      Queries matched: 1
 |||involved in intracellular protein transport|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   409.78   817.55   817.47   0.08 1  9  7.9 7  R.QLKSQSK.N

17.   SPAC3A12.11c                      Score: 9      Queries matched: 1
 |cwf2|prp3|involved in mRNA splicing |Schizosaccharomyces pombe|chr 1|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   409.78   817.55   818.38   -0.83 0  9  8.8 8  K.NNDVTEK.I

18.   SPAC27F1.06c                      Score: 9      Queries matched: 2
 |||peptidyl-prolyl cis-trans isomerase |Schizosaccharomyces pombe|chr 1|||Manual
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   527.36   526.35   528.33   -1.97 0  4  2 2  K.LLAVN.-
 5   421.80   841.59   841.47   0.12 1  4  20 2  K.VKGDGPAAK.R

19.   SPCC5E4.04                        Score: 8      Queries matched: 1
 |cut1||separin|Schizosaccharomyces pombe|chr 3|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   423.78   845.55   845.43   0.13 0  12  3.9 2  K.VSWTPEK.F

20.   SPBC336.08                        Mass: 23082    Score: 8      Queries matched: 1
 |||kinetochore protein |Schizosaccharomyces pombe|chr 2|||Manual
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   523.34   1044.66   1046.51   -1.85 0  8  9.3 1  M.SDSPIELMR.S

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   527.36   526.35   526.26   0.09 0  6  1.2 1  HGTGR
20   737.78   2210.32   2210.05   0.27 2  6  7.6 1  DLIFEMENKKATGNEQSEK
5   421.80   841.59   841.54   0.05 0  5  19 1  ISALGLLR
9   502.86   1003.70   1005.53   -1.83 1  4  19 1  YTYYIKR
14   555.33   1108.65   1110.50   -1.85 0  3  21 1  WQSAFESEK
7   435.82   869.63   869.36   0.27 0  2  35 1  GMSSETMK
10   516.37   1030.73   1029.57   1.16 0  0  53 1  VPEALVSTSK
2   532.94   531.93        
12   533.39   1064.77        
13   546.89   1091.77        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 2 Da
Fragment Mass Tolerance: ± 0.8 Da
Max Missed Cleavages   : 2
Instrument type        : ESI-QUAD-TOF
Number of queries      : 21

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 17 indicates homology
Score greater than 20 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  6.5    0.09                    R.HGTGR.I
  4.3   -1.97   18  SPAC27F1.06c K.LLAVN.-
Top scoring peptide matches to query 2
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 3
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 46.6    0.09    1  SPAC1705.03c K.GALTVETK.A
 16.7    0.09    9  SPBC3D6.12   K.QTIESLK.D
 15.7    0.12    8  SPBC14C8.14c K.KNAQETK.Q
 10.7    0.11                    K.SVGTNGRK.V
  9.8    0.08   13  SPAC23G3.12c R.QYALPVK.G
  9.6    0.08   15  SPBC776.08c  R.QIFPVSK.L
  9.4    0.08   16  SPCC162.06c  R.QLKSQSK.N
  8.8   -0.83   17  SPAC3A12.11c K.NNDVTEK.I
  8.4    0.06                    K.KLITMGR.G
  7.6   -1.83                    K.EQNSNTK.T
Top scoring peptide matches to query 4
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 40.7    0.09    1  SPAC1705.03c K.GVSVNFSK.L
 15.9    0.12    7  SPAPB2B4.03  R.RSCSLSK.F
 12.0    0.08                    K.SRVNSFK.D
 11.6   -1.91   11  SPBC354.08c  K.KHDNAVR.S
  9.7    0.12   14  SPAC1952.08c K.RCLSSSK.Y
  7.7    0.12                    R.GAFDGRSK.E
  7.0   -1.87                    R.HNENVAR.A
  6.3    0.06                    K.QSKLFSK.G
  6.0   -1.93                    K.QEAHKVK.K
  4.5   -0.00                    K.RLPARPK.K
Top scoring peptide matches to query 5
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  4.7    0.05                    K.ISALGLLR.L
  4.3    0.12   18  SPAC27F1.06c K.VKGDGPAAK.R
  2.3    0.17                    K.VTAEYFL.-
  0.7    0.05                    K.SLIALLGR.K
Top scoring peptide matches to query 6
Score greater than 27 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 32.6    0.09    5  mSPCC1322.10 -.VSVTSPTR.D
 12.5    0.13   19  SPCC5E4.04   K.VSWTPEK.F
 12.2   -1.88   10  SPBC32H8.13c K.TVSTPSEK.L
 10.3    0.08                    R.VSDTRLR.L
  7.0    0.12                    M.GEVERTR.Q
  4.6    0.15                    K.AEDAERR.N
  4.2    0.09                    K.KDESVLR.R
  3.4   -1.90                    K.TVSILCR.G
  3.2   -1.90                    K.STSRELR.I
  3.2    1.08                    K.GKTVEVGR.A
Top scoring peptide matches to query 7
Score greater than 13 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.6    0.27        K.GMSSETMK.A
  0.7    1.07        K.ARLQLLR.G
  0.7    1.01        R.KVLKLLR.R
Top scoring peptide matches to query 8
Score greater than 18 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein     Peptide
 30.7    0.11    6  SPAC23A1.10 R.LPLQDVYK.I
  3.4   -0.80                   R.LEEDQNTK.H
  3.1    0.12                   K.IIDTLEGSK.K
  2.9    0.12                   K.IIDVASTEK.Q
Top scoring peptide matches to query 9
Score greater than 16 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  4.4   -1.83        K.YTYYIKR.Q
  3.1    0.15        K.VLSGSLCIR.S
  1.5   -1.77        R.DWSAVCLR.F
Top scoring peptide matches to query 10
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  0.4    1.16        R.VPEALVSTSK.K
Top scoring peptide matches to query 11
Score greater than 19 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
  8.1   -1.85   20  SPBC336.08 M.SDSPIELMR.S
  5.6    0.07                  R.ISKVNSELR.S
  0.7    1.13                  R.TPSPAISESR.K
  0.6    0.14                  K.AEVATPTNSR.S
  0.3    0.07                  R.AESIRQTLK.S
  0.3    0.09                  R.EASKALDRR.Q
  0.3    0.99                  K.KASLSKLGLK.N
  0.3    0.01                  R.SLSLLKVASK.S
Top scoring peptide matches to query 12
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 13
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 14
Score greater than 14 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.0   -1.85        K.WQSAFESEK.L
  1.1   -0.92        K.TLETEYNLK.A
Top scoring peptide matches to query 15
Score greater than 16 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 25.3    0.14    3  mPSU1 K.GTSAQYYVNK.A
  2.1   -0.96             K.KRNISSEVAK.D
Top scoring peptide matches to query 16
Score greater than 14 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 14.4    0.16    2  SPAC11E3.13c K.GNAFFNSDTNER.F
  0.6    0.01                    R.EAGAREAAAAAQKK.K
Top scoring peptide matches to query 17
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 46.8    0.14    4  SPBC32F12.11 R.VPTPDVSVVDLTVK.L
Top scoring peptide matches to query 18
Score greater than 18 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 23.4    0.19    3  mPSU1 K.TQWPSTQPSDGETR.G
  4.2   -1.96             K.AAAGEIKYLSESKPK.I
  3.1   -1.91             K.ASFFDIGGHSILATR.L
  0.9    0.15             R.KTQIEDLEEDQNK.S
  0.5   -1.83             R.QRDDANKGGSSSNQK.K
  0.0   -1.98             K.DKSKSPHIVVATPGR.L
  0.0   -1.91             K.KSKDELWCLISGR.I
Top scoring peptide matches to query 19
Score greater than 19 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 10.0    0.23   12  SPAC13G6.10c R.GLAWIPGTDLGYSDNFVNK.G
  6.4   -0.88                    R.ADLEIQRAAIPIMVAQNSK.L
  3.9   -1.77                    K.QEKEQETDLEAQVEHKK.R
  3.4   -1.93                    R.TLQSLSCARIRPLVMVPK.S
  1.2    1.12                    R.LKAVQLSHQNNYREPIR.A
  1.1   -1.95                    R.ASLLTGVDTLARAVSVTLGPK.G
  1.1   -1.85                    K.GIENEIVDAAQEERVLKR.L
  1.0    0.06                    R.LALSRFLEKPIRYFTGR.T
  1.0    1.17                    R.FHRVIKNFMLQGGDFTR.G
  1.0    1.08                    K.NLRRLDIVFPIPGQYHK.Y
Top scoring peptide matches to query 20
Score greater than 14 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  6.3    0.27        R.DLIFEMENKKATGNEQSEK.G
  0.7   -1.87        K.HYEMLFVRGDSVILIAPPR.N
  0.3    0.15        K.AVEEAATHEPGKKAVAVSAFAK.A
Top scoring peptide matches to query 21
Score greater than 20 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 36.3    0.27    2  SPAC11E3.13c R.GVDYQPGGSSTLVDPLADTSICK.R
  5.5    0.28                    R.TGTFMALFEILSQTDDSTSTSK.F
  4.3    0.30                    R.VSPPPGFCYDVHPHNNIACAK.C
  4.1    0.20                    R.DWTCLDLGGIGLRNVSTDLFK.F
  3.2   -0.70                    K.IFEVSDVAFCDYSRERMTR.N
  2.4    0.17                    R.NMSVIAHVDHGKSTLTDSLVQK.A
  2.4   -0.75                    K.TEWELYDLSQDKGELENLAK.V
  1.9    0.20                    K.LSATQRSSLESFYTSFISLDK.N
  1.4    0.17                    M.ASLDIHLYVKKDLTYGECVR.V
  1.4    1.17                    K.AVFDELCSLVDPKVDAFTPKK.G