Mascot Search Results

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MS data file    : cw0210_q7.pkl
Database        : Cglabrata 041110dos (5427 sequences; 2703904 residues)
Timestamp       : 19 Aug 2005 at 11:48:52 GMT
Significant hits: CAGL0F07579g  similar to sp|P28319 Saccharomyces cerevisiae YKL096w CWP1 cell wall mannoprotein, start by similar
                  CAGL0I06160g  highly similar to sp|P47001 Saccharomyces cerevisiae YJL158c CIS3, start by similarity [Candida gla

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 25 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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1.    CAGL0F07579g                      Mass: 20710    Score: 287    Queries matched: 6
 similar to sp|P28319 Saccharomyces cerevisiae YKL096w CWP1 cell wall mannoprotein, start by similar
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   859.38   1716.75   1715.80   0.95 0  84  9.4e-008 1  K.FSDNTYAVVNSDGSIK.T
3   578.31   1731.89   1731.92   -0.02 0  (54) 8.8e-005 1  K.FSTAQGTGAIDIVISPR.S
4   578.31   1731.91   1731.92   -0.00 0  (44) 0.001 1  K.FSTAQGTGAIDIVISPR.S
5   867.02   1732.02   1731.92   0.11 0  146  5.5e-014 1  K.FSTAQGTGAIDIVISPR.S
8   705.99   2114.95   2114.98   -0.02 0  14  0.62 1  K.TGSESEGTSGFALSGSHLTYK.G
14   1092.19   3273.56   3273.63   -0.07 1  43  0.00034 1  K.GNSGFFAIPSGSEYKFSTAQGTGAIDIVISPR.S

 
      Proteins matching the same set of peptides:
      CAGL0F07601g                      Mass: 21335    Score: 287    Queries matched: 6
 similar to sp|P28319 Saccharomyces cerevisiae YKL096w CWP1 cell wall mannoprotein, start by similar
      mCwp1.1                           Mass: 19256    Score: 287    Queries matched: 6
 |Weig et al. 2004| 198aa Similar to S.cerevisiae YKL096w (CWP1) CAGL0F07601g|CAGL-IPF545|CAGL-CDS42
      mCwp1.2                           Mass: 18631    Score: 287    Queries matched: 6
 |predicted| 192aa Similar to S.cerevisiae YKL096w (CWP1) CAGL0F07579g|CAGL-IPF543|CAGL-CDS4333.1

2.    CAGL0I06160g                      Mass: 24169    Score: 86     Queries matched: 1
 highly similar to sp|P47001 Saccharomyces cerevisiae YJL158c CIS3, start by similarity [Candida gla
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   1023.47   2044.93   2044.98   -0.05 0  86  4e-008 1  K.IAEQCSPINLEVVSLVDC.-

 
      Proteins matching the same set of peptides:
      mPir4p.1                          Mass: 22496    Score: 86     Queries matched: 1
 |predicted-NN| 216aa Highly similar to S.cerevisiae YJL158c (CIS3/PIR4) CAGL0I06160g|CAGL-IPF1401|C
      mPir4p.2                          Mass: 22425    Score: 86     Queries matched: 1
 |predicted-HMM| 215aa Highly similar to S.cerevisiae YJL158c (CIS3/PIR4) CAGL0I06160g|CAGL-IPF1401|
3.    CAGL0M05159g                      Score: 10     Queries matched: 1
 similar to sp|Q02555 Saccharomyces cerevisiae YMR239c RNT1 double-stranded ribonuclease, hypothetical start [Candida glabrata] Complete CDS. CAGL0M05159g|CAGL-IPF7196|CAGL-CDS2404.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   867.02   1732.02   1730.90   1.12 1  10  2.5 2  K.QLKSNLSPDVDLSTSK.T

4.    CAGL0F04257g                      Mass: 39120    Score: 4      Queries matched: 1
 similar to sp|P38199 Saccharomyces cerevisiae YBL032w, hypothetical start [Candida glabrata] Comple
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   696.87   1391.72   1392.83   -1.11 1  4  13 1  -.MPVSHRILLSLK.E

5.    CAGL0H01507g                      Score: 3      Queries matched: 1
 similar to sp|Q06639 Saccharomyces cerevisiae YDR303c RSC3, start by similarity [Candida glabrata] Complete CDS. CAGL0H01507g|CAGL-IPF5820|CAGL-CDS0827.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   696.87   1391.72   1390.68   1.05 0  3  16 2  K.NDLNMEISNITK.H

6.    CAGL0K04807g                      Score: 3      Queries matched: 1
 similar to sp|P40157 Saccharomyces cerevisiae YNL212w, start by similarity [Candida glabrata] Complete CDS. CAGL0K04807g|CAGL-IPF4017|CAGL-CDS0818.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   578.31   1731.89   1732.88   -0.99 0  3  14 2  K.QEISMIPSGEFNLLR.S

7.    CAGL0H01419g                      Score: 2      Queries matched: 1
 similar to tr|Q06631 Saccharomyces cerevisiae YDR299w BFR2 involved in protein transport steps at the Brefeldin A blocks, start by similarity [Candida glabrata] Complete CDS. CAGL0H01419g|CAGL-IPF5815|CAGL-CDS1945.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   859.38   1716.75   1715.90   0.85 1  2  13 2  K.AMNSANVLPVTKASWK.L

8.    CAGL0G10087g                      Mass: 162560   Score: 2      Queries matched: 1
 similar to sp|P17883 Saccharomyces cerevisiae YPR189w SKI3 antiviral protein, start by similarity [
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   1055.86   3164.55   3163.64   0.91 1  2  5.4 1  K.NGSSNSVSLFSHAFVLSNGRSPSIQLLFAK.S

9.    CAGL0F08129g                      Score: 2      Queries matched: 1
 highly similar to sp|P53313 Saccharomyces cerevisiae YGR245c SDA1 required for normal organization of the actin cytoskeleton, start by similarity [Candida glabrata] Complete CDS. CAGL0F08129g|CAGL-IPF588|CAGL-CDS0953.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   859.38   1716.75   1716.67   0.08 1  2  15 3  K.EYDVDMEDSDDEKK.S

10.   CAGL0L13156g                      Score: 2      Queries matched: 1
 some similarities with tr|Q08003 Saccharomyces cerevisiae YLR073c, hypothetical start [Candida glabrata] Complete CDS. CAGL0L13156g|CAGL-IPF8532|CAGL-CDS4427.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   696.87   1391.72   1391.65   0.07 1  2  22 3  K.TELDAMYERHK.V

11.   CAGL0L04466g                      Score: 1      Queries matched: 1
 similar to tr|Q99287 Saccharomyces cerevisiae YOR165w, hypothetical start [Candida glabrata] Complete CDS. CAGL0L04466g|CAGL-IPF8128|CAGL-CDS0852.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   867.02   1732.02   1730.89   1.13 1  1  17 3  K.TAKVEEEIELQDLSK.N

12.   CAGL0F07755g                      Mass: 71670    Score: 1      Queries matched: 1
 similar to sp|P40969 Saccharomyces cerevisiae YMR168c CEP3 kinetochore protein complex, start by si
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   1082.00   2161.99   2161.09   0.91 1  1  13 1  M.FQRPLPTKSNRPCSVCSK.R

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   887.41   1772.80        
10   791.91   3163.60        
12   1062.86   3185.55        
13   1074.53   3220.57        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 14

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 16 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  4.0   -1.11    4  CAGL0F04257g -.MPVSHRILLSLK.E
  2.9    1.05    5  CAGL0H01507g K.NDLNMEISNITK.H
  1.6    0.07   10  CAGL0L13156g K.TELDAMYERHK.V
Top scoring peptide matches to query 2
Score greater than 20 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 83.7    0.95    1  CAGL0F07579g K.FSDNTYAVVNSDGSIK.T
  2.2    0.85    7  CAGL0H01419g K.AMNSANVLPVTKASWK.L
  1.7    0.08    9  CAGL0F08129g K.EYDVDMEDSDDEKK.S
Top scoring peptide matches to query 3
Score greater than 18 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 54.4   -0.02    1  CAGL0F07579g K.FSTAQGTGAIDIVISPR.S
  2.5   -0.99    6  CAGL0K04807g K.QEISMIPSGEFNLLR.S
Top scoring peptide matches to query 4
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 43.8   -0.00    1  CAGL0F07579g K.FSTAQGTGAIDIVISPR.S
Top scoring peptide matches to query 5
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
146.2    0.11    1  CAGL0F07579g K.FSTAQGTGAIDIVISPR.S
  9.6    1.12    3  CAGL0M05159g K.QLKSNLSPDVDLSTSK.T
  1.3    1.13   11  CAGL0L04466g K.TAKVEEEIELQDLSK.N
Top scoring peptide matches to query 6
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 7
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 86.2   -0.05    2  CAGL0I06160g K.IAEQCSPINLEVVSLVDC.-
Top scoring peptide matches to query 8
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 14.2   -0.02    1  CAGL0F07579g K.TGSESEGTSGFALSGSHLTYK.G
Top scoring peptide matches to query 9
Score greater than 13 indicates homology
Score greater than 24 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  0.9    0.91   12  CAGL0F07755g M.FQRPLPTKSNRPCSVCSK.R
Top scoring peptide matches to query 10
Score greater than 21 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 11
Score greater than 14 indicates homology
Score greater than 22 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  1.9    0.91    8  CAGL0G10087g K.NGSSNSVSLFSHAFVLSNGRSPSIQLLFAK.S
Top scoring peptide matches to query 12
Score greater than 21 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 13
Score greater than 21 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 14
Score greater than 21 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 43.0   -0.07    1  CAGL0F07579g K.GNSGFFAIPSGSEYKFSTAQGTGAIDIVISPR.S