Mascot Search Results

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MS data file    : cw0210_q4.pkl
Database        : Cglabrata 041110dos (5427 sequences; 2703904 residues)
Timestamp       : 19 Aug 2005 at 11:44:28 GMT
Significant hits: mCwp1.1       |Weig et al. 2004| 198aa Similar to S.cerevisiae YKL096w (CWP1) CAGL0F07601g|CAGL-IPF545|CAGL-CDS42
                  mCwp1.2       |predicted| 192aa Similar to S.cerevisiae YKL096w (CWP1) CAGL0F07579g|CAGL-IPF543|CAGL-CDS4333.1
                  CAGL0M08492g  highly similar to sp|Q03178 Saccharomyces cerevisiae YKL164c required forlerance to heat shock, sta

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 26 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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1.    mCwp1.1                           Mass: 19256    Score: 359    Queries matched: 6
 |Weig et al. 2004| 198aa Similar to S.cerevisiae YKL096w (CWP1) CAGL0F07601g|CAGL-IPF545|CAGL-CDS42
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   780.86   1559.71   1559.73   -0.01 0  45  0.00084 1  K.GNSGFFAIPSGSEYK.F
5   858.90   1715.78   1715.80   -0.02 0  114  7.7e-011 1  K.FSDNTYAVVNSDGSIK.T
6   866.95   1731.88   1731.92   -0.03 0  100  2.3e-009 1  K.FSTAQGTGAIDIVISPR.S
7   578.31   1731.91   1731.92   -0.00 0  (60) 2.5e-005 1  K.FSTAQGTGAIDIVISPR.S
 11   1058.48   2114.94   2114.98   -0.04 0  2  11 2  K.TGSESEGTSGFALSGSHLTYK.G
13   1055.52   3163.54   3162.63   0.91 0  98  1.2e-009 1  -.DSQAFGLLAIHSGSPVQNTPVDSQNGALVLK.T

2.    mCwp1.2                           Mass: 18631    Score: 350    Queries matched: 6
 |predicted| 192aa Similar to S.cerevisiae YKL096w (CWP1) CAGL0F07579g|CAGL-IPF543|CAGL-CDS4333.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   780.86   1559.71   1559.73   -0.01 0  45  0.00084 1  K.GNSGFFAIPSGSEYK.F
 5   858.90   1715.78   1715.80   -0.02 0  114  7.7e-011 1  K.FSDNTYAVVNSDGSIK.T
 6   866.95   1731.88   1731.92   -0.03 0  100  2.3e-009 1  K.FSTAQGTGAIDIVISPR.S
 7   578.31   1731.91   1731.92   -0.00 0  (60) 2.5e-005 1  K.FSTAQGTGAIDIVISPR.S
 11   1058.48   2114.94   2114.98   -0.04 0  2  11 2  K.TGSESEGTSGFALSGSHLTYK.G
 13   1055.52   3163.54   3162.63   0.91 0  88  1e-008 2  -.DSQAFGLLAIHSGSPVQNTPVNSENGALVLK.S

3.    CAGL0M08492g                      Mass: 34283    Score: 30     Queries matched: 1
 highly similar to sp|Q03178 Saccharomyces cerevisiae YKL164c required forlerance to heat shock, sta
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   559.80   1117.58   1117.58   -0.00 0  30  0.04 1  K.VDGTLQMNLK.G

 
      Proteins matching the same set of peptides:
      mPir3p                            Mass: 32307    Score: 30     Queries matched: 1
 |predicted| 316aa Highly similar to S.cerevisiae YKL164c (Pir1) CAGL0M08492g|CAGL-IPF8003|CAGL-CDS3
4.    CAGL0I06182g                      Mass: 34833    Score: 10     Queries matched: 1
 highly similar to sp|Q03178 Saccharomyces cerevisiae YKL164c PIR1, start by similarity [Candida gla
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   559.80   1117.58   1118.56   -0.99 0  10  3.9 2  K.VDGTLEMNLK.G

 
      Proteins matching the same set of peptides:
      CAGL0I06204g                      Mass: 35969    Score: 10     Queries matched: 1
 highly similar to sp|Q03178 Saccharomyces cerevisiae YKL164c PIR1, start by similarity [Candida gla
      mPir1p                            Score: 10     Queries matched: 1
      mPir2p.1                          Score: 10     Queries matched: 1
      mPir2p.2                          Score: 10     Queries matched: 1
5.    CAGL0L03520g                      Score: 7      Queries matched: 1
 similar to sp|Q01389 Saccharomyces cerevisiae YJL095w BCK1, start by similarity [Candida glabrata] Complete CDS. CAGL0L03520g|CAGL-IPF2711|CAGL-CDS0128.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   858.90   1715.78   1714.90   0.88 0  7  4.3 2  R.AHLDKPVVEELHNSK.S

6.    CAGL0B03179g                      Mass: 102044   Score: 6      Queries matched: 1
 weakly similar to sp|P47035 Saccharomyces cerevisiae YJL076w NET1 required for rDNA silencing and n
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   877.95   1753.89   1754.97   -1.08 1  6  5.2 1  K.AFGNSSRIEPQILVPK.R

7.    CAGL0H02805g                      Score: 5      Queries matched: 1
 similar to sp|P47037 Saccharomyces cerevisiae YJL074c SMC3 required for structural maintenance of chromosomes, start by similarity [Candida glabrata] Complete CDS. CAGL0H02805g|CAGL-IPF1974|CAGL-CDS0226.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   559.80   1117.58   1117.62   -0.04 0  5  12 3  K.TLSSEDLLLK.L

8.    CAGL0A02112g                      Score: 4      Queries matched: 1
 highly similar to sp|P38707 Saccharomyces cerevisiae YHR019c DED81 asparaginyl-tRNA-synthetase, hypothetical start [Candida glabrata] Complete CDS. CAGL0A02112g|CAGL-IPF2985|CAGL-CDS1779.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   877.95   1753.89   1754.98   -1.10 0  4  7.9 2  K.TVQYILEDPIAGPLVK.Q

9.    CAGL0H01419g                      Score: 3      Queries matched: 1
 similar to tr|Q06631 Saccharomyces cerevisiae YDR299w BFR2 involved in protein transport steps at the Brefeldin A blocks, start by similarity [Candida glabrata] Complete CDS. CAGL0H01419g|CAGL-IPF5815|CAGL-CDS1945.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   858.90   1715.78   1715.90   -0.13 1  3  12 3  K.AMNSANVLPVTKASWK.L

10.   CAGL0B00352g                      Score: 2      Queries matched: 1
 highly similar to sp|P25586 Saccharomyces cerevisiae YCL059c KRR1, start by similarity [Candida glabrata] Complete CDS. CAGL0B00352g|CAGL-IPF3677|CAGL-CDS3268.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   877.95   1753.89   1754.91   -1.02 1  4  7.9 2  R.KVDLQIESGEYFLSK.K

11.   CAGL0L02871g                      Mass: 82339    Score: 2      Queries matched: 1
 highly similar to sp|P39744 Saccharomyces cerevisiae YOR206w RAD4, start by similarity [Candida gla
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   1058.48   2114.94   2116.09   -1.15 1  2  9.9 1  K.YAVTDEKAFQELMFIALK.D

12.   CAGL0G10087g                      Score: 1      Queries matched: 1
 similar to sp|P17883 Saccharomyces cerevisiae YPR189w SKI3 antiviral protein, start by similarity [Candida glabrata] Complete CDS. CAGL0G10087g|CAGL-IPF1512|CAGL-CDS0140.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 13   1055.52   3163.54   3163.64   -0.10 1  1  5.1 3  K.NGSSNSVSLFSHAFVLSNGRSPSIQLLFAK.S

13.   CAGL0L04048g                      Score: 1      Queries matched: 1
 similar to sp|P07213 Saccharomyces cerevisiae YNL121c TOM70 or sp|P38825 Saccharomyces cerevisiae YHR117w TOM7, hypothetical start [Candida glabrata] Complete CDS. CAGL0L04048g|CAGL-IPF8101|CAGL-CDS1575.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   1058.48   2114.94   2114.17   0.77 1  1  13 3  K.LHPRPKSYIYLGLLMADK.G

14.   CAGL0A02695g                      Score: 1      Queries matched: 1
 similar to tr|Q06339 Saccharomyces cerevisiae YDR362c TFC6, hypothetical start [Candida glabrata] Complete CDS. CAGL0A02695g|CAGL-IPF1294|CAGL-CDS1122.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   1058.48   2114.94   2116.12   -1.18 1  1  13 4  R.FYSFSISAGIVTIERIGEK.-

15.   CAGL0K01551g                      Score: 1      Queries matched: 1
 highly similar to sp|Q12389 Saccharomyces cerevisiae YDL031w, start by similarity [Candida glabrata] Complete CDS. CAGL0K01551g|CAGL-IPF5090|CAGL-CDS0486.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   1058.48   2114.94   2115.89   -0.95 0  1  13 4  K.VDNDDDDVNAYFSVNTDAK.S

16.   CAGL0L04224g                      Score: 1      Queries matched: 1
 highly similar to sp|Q12487 Saccharomyces cerevisiae YOR150w MRPL23, start by similarity [Candida glabrata] Complete CDS. CAGL0L04224g|CAGL-IPF8112|CAGL-CDS4684.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   1058.48   2114.94   2113.97   0.96 0  1  13 4  K.VFDGAEHPYGDNFTAYALK.Q

17.   CAGL0E02431g                      Score: 0      Queries matched: 1
 highly similar to sp|P04786 Saccharomyces cerevisiae YOL006c TOP1 DNA topoisomerase I, hypothetical start [Candida glabrata] Complete CDS. CAGL0E02431g|CAGL-IPF7961|CAGL-CDS1037.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   1058.48   2114.94   2115.00   -0.06 1  0  15 7  K.DESDDEENHITGLKSSIVK.A

18.   CAGL0J10626g                      Mass: 61075    Score: 0      Queries matched: 1
 similar to sp|P38799 Saccharomyces cerevisiae YHR078w, start by similarity [Candida glabrata] Compl
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   644.81   1287.60   1287.76   -0.16 1  0  31 1  R.SNLPFGTALKLK.E

19.   CAGL0L03784g                      Score: 0      Queries matched: 1
 some similarities with sp|P20676 Saccharomyces cerevisiae YOR098c NUP1 nuclear pore protein, hypothetical start [Candida glabrata] Complete CDS. CAGL0L03784g|CAGL-IPF2682|CAGL-CDS0640.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   559.80   1117.58   1117.53   0.05 0  0  37 4  K.QEATIAENDK.T

20.   CAGL0F07469g                      Mass: 71526    Score: 0      Queries matched: 1
 similar to sp|P38330 Saccharomyces cerevisiae YBR238c or sp|P53140 Saccharomyces cerevisiae YGL107c
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   1058.48   2114.94   2116.14   -1.20 1  0  16 8  K.GIDEPFLNIILAKCTVWK.E

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   680.32   1358.62        
9   885.94   1769.86        
10   895.47   1788.93        
12   705.99   2114.96        
14   791.90   3163.58        
15   1062.85   3185.52        
16   1074.53   3220.56        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 16

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 24 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 29.9   -0.00    3  CAGL0M08492g K.VDGTLQMNLK.G
 10.0   -0.99    4  CAGL0I06182g K.VDGTLEMNLK.G
  5.2   -0.04    7  CAGL0H02805g K.TLSSEDLLLK.L
  0.2    0.05   19  CAGL0L03784g K.QEATIAENDK.T
Top scoring peptide matches to query 2
Score greater than 13 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  0.3   -0.16   18  CAGL0J10626g R.SNLPFGTALKLK.E
Top scoring peptide matches to query 3
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 4
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 44.9   -0.01    1+ mCwp1.1 K.GNSGFFAIPSGSEYK.F
Top scoring peptide matches to query 5
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
114.5   -0.02    1+ mCwp1.1      K.FSDNTYAVVNSDGSIK.T
  7.0    0.88    5  CAGL0L03520g R.AHLDKPVVEELHNSK.S
  2.6   -0.13    9  CAGL0H01419g K.AMNSANVLPVTKASWK.L
Top scoring peptide matches to query 6
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
100.3   -0.03    1+ mCwp1.1 K.FSTAQGTGAIDIVISPR.S
Top scoring peptide matches to query 7
Score greater than 17 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 60.0   -0.00    1+ mCwp1.1 K.FSTAQGTGAIDIVISPR.S
  1.2    0.10               R.KGAGAAGAAGSAGTGGNSDAGK.K
Top scoring peptide matches to query 8
Score greater than 17 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  6.2   -1.08    6  CAGL0B03179g K.AFGNSSRIEPQILVPK.R
  4.4   -1.02   10  CAGL0B00352g R.KVDLQIESGEYFLSK.K
  4.4   -1.10    8  CAGL0A02112g K.TVQYILEDPIAGPLVK.Q
  4.4   -1.02                    K.VDLQIESGEYFLSKK.E
Top scoring peptide matches to query 9
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 10
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 11
Score greater than 14 indicates homology
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  2.1   -1.15   11  CAGL0L02871g K.YAVTDEKAFQELMFIALK.D
  1.7   -0.04    1+ mCwp1.1      K.TGSESEGTSGFALSGSHLTYK.G
  1.1    0.77   13  CAGL0L04048g K.LHPRPKSYIYLGLLMADK.G
  1.0   -1.18   14  CAGL0A02695g R.FYSFSISAGIVTIERIGEK.-
  1.0   -0.95   15  CAGL0K01551g K.VDNDDDDVNAYFSVNTDAK.S
  1.0    0.96   16  CAGL0L04224g K.VFDGAEHPYGDNFTAYALK.Q
  0.3   -0.06   17  CAGL0E02431g K.DESDDEENHITGLKSSIVK.A
  0.2   -1.20   20  CAGL0F07469g K.GIDEPFLNIILAKCTVWK.E
  0.2   -0.14                    K.WFGFGPVDKDGFGIGYIIK.D
  0.0   -0.18                    R.LIDNAISLGTKESLAWAEGK.I
Top scoring peptide matches to query 12
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 13
Score greater than 21 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 97.8    0.91    1  mCwp1.1      -.DSQAFGLLAIHSGSPVQNTPVDSQNGALVLK.T
 88.5    0.91    2  mCwp1.2      -.DSQAFGLLAIHSGSPVQNTPVNSENGALVLK.S
  1.4   -0.10   12  CAGL0G10087g K.NGSSNSVSLFSHAFVLSNGRSPSIQLLFAK.S
Top scoring peptide matches to query 14
Score greater than 21 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 15
Score greater than 21 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 16
Score greater than 21 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide