Mascot Search Results

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MS data file    : cw0210_q3.pkl
Database        : Cglabrata 041110dos (5427 sequences; 2703904 residues)
Timestamp       : 19 Aug 2005 at 11:46:22 GMT
Significant hits: CAGL0F07579g  similar to sp|P28319 Saccharomyces cerevisiae YKL096w CWP1 cell wall mannoprotein, start by similar

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 27 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    CAGL0F07579g                      Mass: 20710    Score: 64     Queries matched: 1
 similar to sp|P28319 Saccharomyces cerevisiae YKL096w CWP1 cell wall mannoprotein, start by similar
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   780.83   1559.64   1559.73   -0.09 0  64  9.3e-006 1  K.GNSGFFAIPSGSEYK.F

 
      Proteins matching the same set of peptides:
      CAGL0F07601g                      Mass: 21335    Score: 64     Queries matched: 1
 similar to sp|P28319 Saccharomyces cerevisiae YKL096w CWP1 cell wall mannoprotein, start by similar
      mCwp1.1                           Mass: 19256    Score: 64     Queries matched: 1
 |Weig et al. 2004| 198aa Similar to S.cerevisiae YKL096w (CWP1) CAGL0F07601g|CAGL-IPF545|CAGL-CDS42
      mCwp1.2                           Mass: 18631    Score: 64     Queries matched: 1
 |predicted| 192aa Similar to S.cerevisiae YKL096w (CWP1) CAGL0F07579g|CAGL-IPF543|CAGL-CDS4333.1

2.    CAGL0L11374g                      Mass: 62612    Score: 5      Queries matched: 1
 similar to sp|P54838 Saccharomyces cerevisiae YML070w DAK1 dihydroxyacetone kinase, start by simila
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   586.28   1170.54   1169.64   0.90 0  5  11 1  R.VIAVGDDVAVGR.E

3.    CAGL0H09152g                      Mass: 86062    Score: 4      Queries matched: 1
 similar to sp|P47116 Saccharomyces cerevisiae YJR059w PTK2, hypothetical start [Candida glabrata] C
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   680.29   1358.56   1358.65   -0.09 0  4  12 1  R.SIHSNATSTGTAGR.K

4.    CAGL0J02640g                      Mass: 63231    Score: 2      Queries matched: 1
 similar to sp|P43609 Saccharomyces cerevisiae YFR037c RSC8, hypothetical start [Candida glabrata] C
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   586.28   1170.54   1170.62   -0.08 1  2  19 2  K.MTVKYFNLR.N

5.    CAGL0L11660g                      Score: 1      Queries matched: 1
 highly similar to sp|P04051 Saccharomyces cerevisiae YOR116c RPO31 DNA-directed RNA polymerase III, start by similarity [Candida glabrata] Complete CDS. CAGL0L11660g|CAGL-IPF4252|CAGL-CDS0119.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   586.28   1170.54   1170.55   -0.00 1  1  26 3  K.TAETGYMSRR.L

6.    CAGL0G09361g                      Score: 1      Queries matched: 1
 similar to sp|P16451 Saccharomyces cerevisiae YGR193c PDX1 pyruvate dehydrogenase complex protein X, hypothetical start [Candida glabrata] Complete CDS. CAGL0G09361g|CAGL-IPF1564|CAGL-CDS2569.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   586.28   1170.54   1171.69   -1.14 1  1  27 4  -.MLSARVLLNR.S

7.    CAGL0E01111g                      Mass: 137210   Score: 0      Queries matched: 1
 similar to sp|P46674 Saccharomyces cerevisiae YDR159w leucine permease transcriptional regulator, h
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   644.77   1287.52   1288.68   -1.15 1  0  29 1  K.GRDFSPLQLEK.C

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   868.41   867.40        
3   636.26   1270.51        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 6

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 2
Score greater than 15 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  4.8    0.90    2  CAGL0L11374g R.VIAVGDDVAVGR.E
  2.3   -0.08    4  CAGL0J02640g K.MTVKYFNLR.N
  1.1   -0.00    5  CAGL0L11660g K.TAETGYMSRR.L
  0.8   -1.14    6  CAGL0G09361g -.MLSARVLLNR.S
Top scoring peptide matches to query 3
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 4
Score greater than 13 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  0.4   -1.15    7  CAGL0E01111g K.GRDFSPLQLEK.C
Top scoring peptide matches to query 5
Score greater than 16 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  3.6   -0.09    3  CAGL0H09152g R.SIHSNATSTGTAGR.K
Top scoring peptide matches to query 6
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 64.0   -0.09    1  CAGL0F07579g K.GNSGFFAIPSGSEYK.F