Mascot Search Results

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MS data file    : cw0129_q3.pkl
Database        : Cglabrata 041110dos (5427 sequences; 2703904 residues)
Timestamp       : 22 Feb 2005 at 14:10:37 GMT
Significant hits: CAGL0M01826g  highly similar to sp|P38248 Saccharomyces cerevisiae YBR078w ECM33 or sp|Q12355 Saccharomyces cerev
                  Glu-C         |Endoproteinase- mature protein - (Staphylococcal serine proteinase) ACC P04188

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 28 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

Switch to Protein Summary Report

To create a bookmark for this report, right click this link: Peptide Summary Report (../data/20050222/F002687.dat)

        Error tolerant   

1.    CAGL0M01826g                      Mass: 43265    Score: 42     Peptides matched: 1
 highly similar to sp|P38248 Saccharomyces cerevisiae YBR078w ECM33 or sp|Q12355 Saccharomyces cerev
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   526.77   1051.54   1051.52   0.02 1  42  0.0022 1  SVEGFGSLKE

 
      Proteins matching the same set of peptides:
      mCAGL0M01826g                     Mass: 41542    Score: 42     Peptides matched: 1
 |predicted| 404aa Highly similar to 38248 S.cerevisiae YBR078w ECM33 CAGL0M01826g|CAGL-IPF6362|CAGL

2.    Glu-C                             Mass: 29006    Score: 30     Peptides matched: 2
 |Endoproteinase- mature protein - (Staphylococcal serine proteinase) ACC P04188
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   559.32   1116.63   1116.62   0.01 0  9  4.9 1  LAIVKFSPNE
8   733.84   1465.67   1465.67   0.00 0  20  0.24 1  LSTTGGNSGSPVFNE

 
      Proteins matching the same set of peptides:
      Glu-C/V8                          Mass: 36304    Score: 30     Peptides matched: 2
 |Endoproteinase precursor (Staphylococcal serine proteinase) ACC P04188
3.    CAGL0F01287g                      Mass: 52284    Score: 28     Peptides matched: 1
 highly similar to sp|Q08193 Saccharomyces cerevisiae YOL030w, hypothetical start [Candida glabrata]
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   708.33   1414.64   1414.64   0.00 1  30  0.036 1  IKGNAFFDSSSNE

 
      Proteins matching the same set of peptides:
      mCAGL0F01287g                     Mass: 50421    Score: 28     Peptides matched: 1
 |predicted| highly similar to sp|Q08193 Saccharomyces cerevisiae YOL030w, hypothetical start [Candi
4.    CAGL0I09174g                      Mass: 18053    Score: 14     Peptides matched: 1
 similar to sp|P38337 Saccharomyces cerevisiae YBR258c, hypothetical start [Candida glabrata] Comple
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   416.74   831.46   831.47   -0.01 0  14  2.3 1  ALKLSVSD

5.    CAGL0K12386g                      Mass: 94886    Score: 14     Peptides matched: 1
 similar to sp|P43572 Saccharomyces cerevisiae YFL024c EPL1, hypothetical start [Candida glabrata] C
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   491.72   981.42   981.52   -0.09 1  14  1.5 1  FIDFKAIE

6.    CAGL0H07711g                      Mass: 66660    Score: 11     Peptides matched: 1
 highly similar to sp|P53064 Saccharomyces cerevisiae YGL244w RTF1, start by similarity [Candida gla
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   416.74   831.46   831.45   0.01 0  11  4.3 2  KQLMLAE

7.    CAGL0F05467g                      Mass: 17381    Score: 11     Peptides matched: 1
 similar to tr|Q03954 Saccharomyces cerevisiae YDR201w SPC19, hypothetical start [Candida glabrata]
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   416.74   831.46   831.43   0.03 0  11  4.6 3  AIAIASATD

8.    CAGL0J10516g                      Score: 10     Peptides matched: 1
 similar to sp|Q04089 Saccharomyces cerevisiae YDR440w DOT1, hypothetical start [Candida glabrata] Complete CDS. CAGL0J10516g|CAGL-IPF2839|CAGL-CDS1260.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   491.72   981.42   980.49   0.93 0  10  4 2  ASTPVHQLE

9.    CAGL0K01749g                      Score: 8      Peptides matched: 1
 similar to sp|Q12451 Saccharomyces cerevisiae YDL019c, start by similarity [Candida glabrata] Complete CDS. CAGL0K01749g|CAGL-IPF8701|CAGL-CDS0212.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   526.77   1051.54   1050.52   1.02 0  10  3.4 2  TATSTASVKGE

10.   CAGL0G02717g                      Score: 8      Peptides matched: 1
 similar to sp|P08019 Saccharomyces cerevisiae YIL099w SGA1 sporulation specific glucan 1, 4-alpha-glucosidase, hypothetical start [Candida glabrata] Complete CDS. CAGL0G02717g|CAGL-IPF3584|CAGL-CDS1979.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   491.72   981.42   981.53   -0.11 0  8  5.6 4  FWKFIIE

11.   CAGL0L00429g                      Score: 8      Peptides matched: 1
 highly similar to sp|P49095 Saccharomyces cerevisiae YMR189w GSD2, hypothetical start [Candida glabrata] Complete CDS. CAGL0L00429g|CAGL-IPF7539|CAGL-CDS0398.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   491.72   981.42   980.45   0.98 1  8  5.6 5  GTTADQFLE

12.   CAGL0M13079g                      Score: 8      Peptides matched: 1
 similar to sp|P35734 Saccharomyces cerevisiae YKL052c, hypothetical start [Candida glabrata] Complete CDS. CAGL0M13079g|CAGL-IPF6106|CAGL-CDS4420.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   491.72   981.42   981.42   0.01 1  8  5.6 5  ANAERSYGD

13.   CAGL0G01254g                      Score: 8      Peptides matched: 1
 highly similar to sp|P53090 Saccharomyces cerevisiae YGL202w ARO8, start by similarity [Candida glabrata] Complete CDS. CAGL0G01254g|CAGL-IPF3866|CAGL-CDS2054.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   526.77   1051.54   1052.59   -1.05 0  8  5.4 3  TSKFSVKKE

14.   CAGL0I08195g                      Score: 8      Peptides matched: 1
 similar to sp|P10823 Saccharomyces cerevisiae YER020w GPA2 guanine nucleotide-binding regulatory protein, start by similarity [Candida glabrata] Complete CDS. CAGL0I08195g|CAGL-IPF4329|CAGL-CDS2414.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   491.72   981.42   981.60   -0.17 0  8  5.7 7  KIQRVPLE

15.   CAGL0G09867g                      Mass: 74687    Score: 7      Peptides matched: 1
 similar to sp|Q06623 Saccharomyces cerevisiae YPR179c PLO1, start by similarity [Candida glabrata]
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   491.72   981.42   981.44   -0.02 1  9  4.5 3  KNSNDYLE

16.   CAGL0H03949g                      Mass: 45354    Score: 7      Peptides matched: 1
 highly similar to sp|P33309 Saccharomyces cerevisiae YNL001w DOM34, start by similarity [Candida gl
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   491.20   980.38   979.50   0.88 0  7  6.7 1  KITQYAQE

17.   CAGL0M05599g                      Score: 7      Peptides matched: 1
 similar to sp|P38288 Saccharomyces cerevisiae YBR162c TOS1, hypothetical start [Candida glabrata] Complete CDS. CAGL0M05599g|CAGL-IPF7164|CAGL-CDS2435.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   491.20   980.38   981.38   -1.00 0  7  7.4 2  TNGNGYNQD

18.   CAGL0L02827g                      Score: 6      Peptides matched: 1
 similar to sp|P29461 Saccharomyces cerevisiae YOR208w PTP2 protein-tyrosine-phosphatase, hypothetical start [Candida glabrata] Complete CDS. CAGL0L02827g|CAGL-IPF2764|CAGL-CDS1141.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   491.20   980.38   980.49   -0.12 1  6  8.1 3  ELRKGTYD

19.   CAGL0F05621g                      Mass: 40832    Score: 6      Peptides matched: 1
 similar to sp|P53688 Saccharomyces cerevisiae YDR191w HST4, hypothetical start [Candida glabrata] C
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   526.77   1051.54   1051.52   0.02 1  6  8.4 4  SVASGKELFD

20.   CAGL0A03432g                      Mass: 132948   Score: 6      Peptides matched: 1
 similar to sp|P32849 Saccharomyces cerevisiae YLR032w RAD5, hypothetical start [Candida glabrata] C
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   491.20   980.38   980.46   -0.08 1  6  8.5 4  ELPTQHQE

Peptide matches not assigned to protein hits: (no details means no match)
List sorted by Decreasing Score. Sort by Decreasing Intensity or Increasing Query # / Mr

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   446.15   445.14         
9   1045.10   3132.29         

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : V8-DE
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Data File Name         : cw0129_q3.pkl
Number of queries      : 9

Mascot:  http://www.matrixscience.com/ 

Top scoring peptide matches to query 1
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
Top scoring peptide matches to query 2
Score greater than 25 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 14.0   -0.01   4                      CAGL0I09174g  ALKLSVSD
 11.3    0.01   6                      CAGL0H07711g  KQLMLAE
 11.0    0.03   7                      CAGL0F05467g  AIAIASATD
  4.2    0.02                                        ALRSEKE
  3.6    0.06                                        SPVSVQSE
  3.4   -0.03                                        AIKLMKE
  2.9    0.01                                        AIAKFAPD
  2.8    0.01                                        AILCLKD
  2.4   -0.93                                        KGDGSGLAE
  1.8    0.04                                        RGRLDSE
Top scoring peptide matches to query 3
Score greater than 19 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  7.1    0.88  16                      CAGL0H03949g  KITQYAQE
  6.7   -1.00  17                      CAGL0M05599g  TNGNGYNQD
  6.3   -0.12  18                      CAGL0L02827g  ELRKGTYD
  6.0   -0.08  20                      CAGL0A03432g  ELPTQHQE
  5.1   -0.10                                        FTKTENLE
  4.5   -0.07                                        ELSSAKMSE
  3.4   -0.15                                        EINVLHKE
  1.3   -0.03                                        NSSSSQDKE
  0.6   -0.05                                        EMLATCKE
Top scoring peptide matches to query 4
Score greater than 23 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 13.7   -0.09   5                      CAGL0K12386g  FIDFKAIE
  9.5    0.93   8                      CAGL0J10516g  ASTPVHQLE
  9.1   -0.02  15                      CAGL0G09867g  KNSNDYLE
  8.1   -0.11  10                      CAGL0G02717g  FWKFIIE
  8.1    0.98  11                      CAGL0L00429g  GTTADQFLE
  8.1    0.01  12                      CAGL0M13079g  ANAERSYGD
  8.0   -0.17  14                      CAGL0I08195g  KIQRVPLE
  5.6   -0.02                                        EMNSFLIE
  4.3    0.94                                        FTKTENLE
  4.2    0.98                                        IYNNLSEE
Top scoring peptide matches to query 5
Score greater than 25 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 42.0    0.02   1                      CAGL0M01826g  SVEGFGSLKE
 10.1    1.02   9                      CAGL0K01749g  TATSTASVKGE
  8.1   -1.05  13                      CAGL0G01254g  TSKFSVKKE
  6.2    0.02  19                      CAGL0F05621g  SVASGKELFD
  5.9    1.02                                        ASSVSTGSVKE
  2.4   -0.00                                        KIMEQFKE
  2.2    0.04                                        SVSKFNQQD
  0.7    1.01                                        NAELLTYVE
  0.7    0.96                                        GKEKKVTFD
  0.2    0.02                                        KDLLYSAGAD
Top scoring peptide matches to query 6
Score greater than 13 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  9.1    0.01   2                             Glu-C  LAIVKFSPNE
Top scoring peptide matches to query 7
Score greater than 17 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 29.5    0.00   3                      CAGL0F01287g  IKGNAFFDSSSNE
  2.2   -0.96                                        SNYYGRTNTPDE
Top scoring peptide matches to query 8
Score greater than 14 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 20.5    0.00   2                             Glu-C  LSTTGGNSGSPVFNE
Top scoring peptide matches to query 9
Score greater than 19 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide