Mascot Search Results

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MS data file    : cw0106_q2.pkl
Database        : Cglabrata 041110dos (5427 sequences; 2703904 residues)
Timestamp       : 22 Feb 2005 at 14:09:14 GMT
Significant hits: CAGL0F07601g  similar to sp|P28319 Saccharomyces cerevisiae YKL096w CWP1 cell wall mannoprotein, start by similar
                  CAGL0F07579g  similar to sp|P28319 Saccharomyces cerevisiae YKL096w CWP1 cell wall mannoprotein, start by similar
                  CAGL0I06160g  highly similar to sp|P47001 Saccharomyces cerevisiae YJL158c CIS3, start by similarity [Candida gla
                  CAGL0M08492g  highly similar to sp|Q03178 Saccharomyces cerevisiae YKL164c required forlerance to heat shock, sta
                  mSSr1         |validated by us| CAGL0H06413g similar to sp|O13547 Saccharomyces cerevisiae YLR390wa CW14, start b
                  CAGL0F01463g  similar to sp|P10863 Saccharomyces cerevisiae YER011w TIR1, start by similarity [Candida glabrata] 
                  CAGL0M01826g  highly similar to sp|P38248 Saccharomyces cerevisiae YBR078w ECM33 or sp|Q12355 Saccharomyces cerev

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 27 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

Switch to Protein Summary Report

To create a bookmark for this report, right click this link: Peptide Summary Report (../data/20050222/F002684.dat)

        Error tolerant   

1.    CAGL0F07601g                      Mass: 21335    Score: 435    Peptides matched: 5
 similar to sp|P28319 Saccharomyces cerevisiae YKL096w CWP1 cell wall mannoprotein, start by similar
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   634.82   1267.62   1267.60   0.01 0  119  4e-011 1  TGGSFAGTVTDAGK
12   503.94   1508.80   1508.78   0.02 1  101  1.6e-009 1  TGGSFAGTVTDAGKLK
13   780.88   1559.74   1559.73   0.01 0  59  3.1e-005 1  GNSGFFAIPSGSEYK
16   858.91   1715.80   1715.80   0.00 0  122  1.3e-011 1  FSDNTYAVVNSDGSIK
18   706.00   2114.98   2114.98   0.01 0  33  0.0084 1  TGSESEGTSGFALSGSHLTYK

 
      Proteins matching the same set of peptides:
      mCwp1.1                           Mass: 19256    Score: 435    Peptides matched: 5
 |Weig et al. 2004| 198aa Similar to S.cerevisiae YKL096w (CWP1) CAGL0F07601g|CAGL-IPF545|CAGL-CDS42


2.    CAGL0F07579g                      Mass: 20710    Score: 384    Peptides matched: 5
 similar to sp|P28319 Saccharomyces cerevisiae YKL096w CWP1 cell wall mannoprotein, start by similar
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   627.81   1253.60   1253.59   0.01 0  108  4.8e-010 1  SGGSFAGTVTDAGK
11   499.27   1494.78   1494.77   0.01 1  61  1.8e-005 1  SGGSFAGTVTDAGKLK
 13   780.88   1559.74   1559.73   0.01 0  59  3.1e-005 1  GNSGFFAIPSGSEYK
 16   858.91   1715.80   1715.80   0.00 0  122  1.3e-011 1  FSDNTYAVVNSDGSIK
 18   706.00   2114.98   2114.98   0.01 0  33  0.0084 1  TGSESEGTSGFALSGSHLTYK

 
      Proteins matching the same set of peptides:
      mCwp1.2                           Mass: 18631    Score: 384    Peptides matched: 5
 |predicted| 192aa Similar to S.cerevisiae YKL096w (CWP1) CAGL0F07579g|CAGL-IPF543|CAGL-CDS4333.1

3.    CAGL0I06160g                      Mass: 24169    Score: 157    Peptides matched: 2
 highly similar to sp|P47001 Saccharomyces cerevisiae YJL158c CIS3, start by similarity [Candida gla
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   551.81   1101.61   1101.60   0.01 0  82  2.5e-007 1  NDGTLQLTLK
15   565.29   1692.85   1692.84   0.01 1  75  7.2e-007 1  ESSCKNDGTLQLTLK

 
      Proteins matching the same set of peptides:
      mPir4p.1                          Mass: 22496    Score: 157    Peptides matched: 2
 |predicted-NN| 216aa Highly similar to S.cerevisiae YJL158c (CIS3/PIR4) CAGL0I06160g|CAGL-IPF1401|C
      mPir4p.2                          Mass: 22425    Score: 157    Peptides matched: 2
 |predicted-HMM| 215aa Highly similar to S.cerevisiae YJL158c (CIS3/PIR4) CAGL0I06160g|CAGL-IPF1401|

4.    CAGL0M08492g                      Mass: 34283    Score: 90     Peptides matched: 1
 highly similar to sp|Q03178 Saccharomyces cerevisiae YKL164c required forlerance to heat shock, sta
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   559.80   1117.59   1117.58   0.01 0  90  3.6e-008 1  VDGTLQMNLK

 
      Proteins matching the same set of peptides:
      mPir3p                            Mass: 32307    Score: 90     Peptides matched: 1
 |predicted| 316aa Highly similar to S.cerevisiae YKL164c (Pir1) CAGL0M08492g|CAGL-IPF8003|CAGL-CDS3

5.    mSSr1                             Mass: 19214    Score: 86     Peptides matched: 1
 |validated by us| CAGL0H06413g similar to sp|O13547 Saccharomyces cerevisiae YLR390wa CW14, start b
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
14   807.93   1613.84   1613.83   0.01 0  86  6.3e-008 1  TPPACLLACVAQVSK


6.    CAGL0F01463g                      Mass: 21450    Score: 38     Peptides matched: 1
 similar to sp|P10863 Saccharomyces cerevisiae YER011w TIR1, start by similarity [Candida glabrata]
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   420.77   839.52   839.51   0.01 0  38  0.0049 1  GIEALLPK

 
      Proteins matching the same set of peptides:
      mCAGL0F01463g                     Mass: 19557    Score: 38     Peptides matched: 1
 |predicted| 202aa Similar to P10863 S. cerevisiae YER011w TIR1 CAGL0F01463g|CAGL-IPF9579|CAGL-CDS4

7.    CAGL0M01826g                      Mass: 43265    Score: 34     Peptides matched: 1
 highly similar to sp|P38248 Saccharomyces cerevisiae YBR078w ECM33 or sp|Q12355 Saccharomyces cerev
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   649.86   1297.70   1297.67   0.02 0  35  0.008 1  EVNVLNINNNR

 
      Proteins matching the same set of peptides:
      mCAGL0M01826g                     Mass: 41542    Score: 34     Peptides matched: 1
 |predicted| 404aa Highly similar to 38248 S.cerevisiae YBR078w ECM33 CAGL0M01826g|CAGL-IPF6362|CAGL

8.    CAGL0I06182g                      Score: 21     Peptides matched: 1
 highly similar to sp|Q03178 Saccharomyces cerevisiae YKL164c PIR1, start by similarity [Candida glabrata] Complete CDS. CAGL0I06182g|CAGL-IPF1404|CAGL-CDS3232.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   559.80   1117.59   1118.56   -0.98 0  21  0.32 2  VDGTLEMNLK

 
      Proteins matching the same set of peptides:
      CAGL0I06204g                      Mass: 35969    Score: 21     Peptides matched: 1
 highly similar to sp|Q03178 Saccharomyces cerevisiae YKL164c PIR1, start by similarity [Candida gla
      mPir1p                            Mass: 34149    Score: 21     Peptides matched: 1
 |predicted| 331aa Highly similar S. cerevisiae YKL164c PIR1 CAGL0I06204g|CAGL-IPF1408|CAGL-CDS3158.
      mPir2p.1                          Score: 21     Peptides matched: 1
      mPir2p.2                          Score: 21     Peptides matched: 1

9.    CAGL0A00979g                      Score: 15     Peptides matched: 1
 no similarity, hypothetical start [Candida glabrata] Complete CDS. CAGL0A00979g|CAGL-IPF15755
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   559.80   1117.59   1116.59   1.00 1  15  1.3 3  RNDVQSATVK


10.   CAGL0H02805g                      Score: 14     Peptides matched: 1
 similar to sp|P47037 Saccharomyces cerevisiae YJL074c SMC3 required for structural maintenance of chromosomes, start by similarity [Candida glabrata] Complete CDS. CAGL0H02805g|CAGL-IPF1974|CAGL-CDS0226.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   559.80   1117.59   1117.62   -0.04 0  14  1.4 4  TLSSEDLLLK


11.   CAGL0H03421g                      Mass: 41768    Score: 13     Peptides matched: 1
 weakly similar to sp|P53159 Saccharomyces cerevisiae YGL075c MPS2, start by similarity [Candida gla
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   551.81   1101.61   1101.60   0.01 1  13  1.8 2  SKADSLGSPIK


12.   CAGL0C01551g                      Score: 12     Peptides matched: 1
 similar to sp|P41817 Saccharomyces cerevisiae YPL177c CUP9, hypothetical start [Candida glabrata] Complete CDS. CAGL0C01551g|CAGL-IPF7256|CAGL-CDS4245.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   551.81   1101.61   1100.68   0.93 1  12  2.6 3  DLLIKTGLTK


13.   CAGL0I03674g                      Mass: 52017    Score: 11     Peptides matched: 1
 highly similar to sp|Q12250 Saccharomyces cerevisiae YDL147w RPN5, start by similarity [Candida gla
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   577.30   1152.58   1152.60   -0.02 1  11  2.5 1  AEKDYTSVLK


14.   CAGL0K08514g                      Mass: 167185   Score: 10     Peptides matched: 1
 similar to sp|P34244 Saccharomyces cerevisiae YKL101w serine/threonine-protein kinase, hypothetical
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   634.82   1267.62   1268.67   -1.06 1  12  1.9 2  SLYSQTSISKR


15.   CAGL0F04565g                      Score: 10     Peptides matched: 1
 similar to sp|P07256 Saccharomyces cerevisiae YBL045c COR1 ubiquinol--cytochrome-c reductase, start by similarity [Candida glabrata] Complete CDS. CAGL0F04565g|CAGL-IPF1671|CAGL-CDS2358.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   551.81   1101.61   1100.64   0.97 1  10  3.8 5  IDAITVKDVK


16.   CAGL0K02365g                      Score: 10     Peptides matched: 1
 highly similar to tr|Q12482 Saccharomyces cerevisiae YPR021c, start by similarity [Candida glabrata] Complete CDS. CAGL0K02365g|CAGL-IPF2266|CAGL-CDS0570.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   551.81   1101.61   1100.62   1.00 1  12  2.6 3  DIREQVITK


17.   CAGL0H00330g                      Score: 10     Peptides matched: 1
 similar to tr|Q06179 Saccharomyces cerevisiae YLR454w, hypothetical start [Candida glabrata] Complete CDS. CAGL0H00330g|CAGL-IPF7288|CAGL-CDS0011.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   559.80   1117.59   1117.56   0.03 0  10  4.2 5  VDNNTSEVLK


18.   CAGL0H02673g                      Score: 10     Peptides matched: 1
 similar to sp|P36144 Saccharomyces cerevisiae YKR060w, hypothetical start [Candida glabrata] Complete CDS. CAGL0H02673g|CAGL-IPF8751|CAGL-CDS3854.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   551.81   1101.61   1101.66   -0.04 1  10  4.1 6  DMRILLITK


19.   CAGL0J01045g                      Score: 10     Peptides matched: 1
 highly similar to sp|P20448 Saccharomyces cerevisiae YJL033w HCA4, start by similarity [Candida glabrata] Complete CDS. CAGL0J01045g|CAGL-IPF7892|CAGL-CDS0899.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   551.81   1101.61   1100.64   0.97 1  10  4.2 7  DLPISKSTLK


20.   CAGL0L07634g                      Score: 9      Peptides matched: 1
 some similarities with sp|Q04263 Saccharomyces cerevisiae YML002w, hypothetical start [Candida glabrata] Complete CDS. CAGL0L07634g|CAGL-IPF9327|CAGL-CDS0289.1
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   444.70   887.38   887.42   -0.04 0  9  5.8 2  DLLCDPR


Peptide matches not assigned to protein hits: (no details means no match)
List sorted by Decreasing Score. Sort by Decreasing Intensity or Increasing Query # / Mr


      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   444.70   887.38   887.49   -0.11 0  11  4.4 1  DIIQMIR
17   585.59   1753.75   1753.74   0.01 0  3  11 1  FESQSNDNELNENSK
6   616.80   1231.58         
10   436.19   1305.56         

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Data File Name         : cw0106_q2.pkl
Number of queries      : 18

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 24 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
37.8 0.01 6 CAGL0F01463g GIEALLPK
8.7 -0.95 ENQLIPK
5.2 -0.92 AAVLENPQ
4.0 -0.99 GIALTAAPK
2.4 0.04 EEPKLPK
2.4 -0.01 KVQQIPK
2.3 0.03 QQQVIPK
2.1 -0.99 GLGSLIPGK
1.8 -0.01 GLVTPVVR
1.1 0.97 AVLRRPK
Top scoring peptide matches to query 2
Score greater than 23 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
10.6 -0.11 DIIQMIR
9.4 -0.04 20 CAGL0L07634g DLLCDPR
8.3 -0.16 LDSISLIK
8.3 -0.09 LDPSTSLR
8.3 -0.09 IDQQEKK
8.3 -0.12 LDSLAIEK
8.3 -0.09 IDDLREK
8.3 -0.09 IDEEVKR
8.0 -0.07 LDGDGAGRK
7.9 -0.09 LVEERDK
Top scoring peptide matches to query 3
Score greater than 28 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
81.8 0.01 3 CAGL0I06160g NDGTLQLTLK
13.2 0.01 11 CAGL0H03421g SKADSLGSPIK
11.7 0.93 12 CAGL0C01551g DLLIKTGLTK
11.7 1.00 16 CAGL0K02365g DIREQVITK
10.0 0.97 15 CAGL0F04565g IDAITVKDVK
9.6 -0.04 18 CAGL0H02673g DMRILLITK
9.6 0.97 19 CAGL0J01045g DLPISKSTLK
8.0 -1.03 SVTVSRISVR
8.0 1.00 LDETILRNK
7.3 0.97 LGKVEDVTIK
Top scoring peptide matches to query 4
Score greater than 28 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
90.3 0.01 4 CAGL0M08492g VDGTLQMNLK
20.8 -0.98 8 CAGL0I06182g VDGTLEMNLK
14.9 1.00 9 CAGL0A00979g RNDVQSATVK
14.4 -0.04 10 CAGL0H02805g TLSSEDLLLK
9.7 0.03 17 CAGL0H00330g VDNNTSEVLK
8.6 -0.03 TLKGVMDNIK
7.3 0.06 QEATIAENDK
7.2 1.02 LEELEAQSAK
7.2 -0.04 ATEHHLGLLK
7.1 -0.00 VELSNRCIK
Top scoring peptide matches to query 5
Score greater than 22 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
11.1 -0.02 13 CAGL0I03674g AEKDYTSVLK
9.0 1.07 MSSDSGDNVLK
7.9 0.04 FVDDSEKTGR
7.0 0.93 QIQPDLPTLK
5.7 -0.02 AFIRTFQDR
5.4 0.99 HNPEKEASIK
5.0 0.95 VYSNRSSVIK
4.4 1.05 RFDAQTGSDR
3.4 0.99 TTSRCSSIIK
2.3 -0.01 KYLFNSPER
Top scoring peptide matches to query 6
Score greater than 28 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
Top scoring peptide matches to query 7
Score greater than 27 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
107.9 0.01 2 CAGL0F07579g SGGSFAGTVTDAGK
8.8 0.92 FSAEALSFLIR
7.9 1.01 NVYETVTGDQK
7.8 -1.14 AINRAGNSVIIK
5.0 -0.00 EYGDVTVRMGK
4.3 0.01 YDAVVEACSLK
2.2 -1.06 TFELSKEFVR
0.8 1.02 RNMSDSTSLSR
0.7 -1.00 LCYDSKISNR
Top scoring peptide matches to query 8
Score greater than 27 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
118.8 0.01 1 CAGL0F07601g TGGSFAGTVTDAGK
12.0 -1.06 14 CAGL0K08514g SLYSQTSISKR
8.3 1.01 GTGDCKFLQNK
6.3 0.04 SSGMSAPTTQSSK
2.0 -1.04 YPGSKTNLSFR
0.6 0.11 EEYWDNNNGK
Top scoring peptide matches to query 9
Score greater than 15 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
35.5 0.02 7 CAGL0M01826g EVNVLNINNNR
0.3 -1.03 IVDDLVNRLSR
Top scoring peptide matches to query 10
Score greater than 27 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
Top scoring peptide matches to query 11
Score greater than 16 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
61.3 0.01 2 CAGL0F07579g SGGSFAGTVTDAGKLK
Top scoring peptide matches to query 12
Score greater than 19 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
101.3 0.02 1 CAGL0F07601g TGGSFAGTVTDAGKLK
Top scoring peptide matches to query 13
Score greater than 16 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
59.2 0.01 1+ CAGL0F07601g GNSGFFAIPSGSEYK
Top scoring peptide matches to query 14
Score greater than 26 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
86.2 0.01 5 mSSr1 TPPACLLACVAQVSK
7.8 0.07 TPNVFDKSGQSSGYK
4.4 0.08 MSDEVAVPPVQEGEK
Top scoring peptide matches to query 15
Score greater than 18 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
75.2 0.01 3 CAGL0I06160g ESSCKNDGTLQLTLK
0.8 -1.07 VQKGGALYYINNNLK
0.0 1.12 TNISNNDNNDDDTIK
Top scoring peptide matches to query 16
Score greater than 26 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
122.2 0.00 1+ CAGL0F07601g FSDNTYAVVNSDGSIK
6.4 -0.10 AMNSANVLPVTKASWK
Top scoring peptide matches to query 17
Score greater than 13 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
2.6 0.01 FESQSNDNELNENSK
2.5 0.94 RNFTSDSNNQGSLGTR
0.3 -1.16 IWINYTCAKPSFKK
Top scoring peptide matches to query 18
Score greater than 15 indicates homology
Score greater than 25 indicates identity
Status bar shows all hits for this peptide

Score Delta Hit Protein Peptide
33.0 0.01 1+ CAGL0F07601g TGSESEGTSGFALSGSHLTYK