Mascot Search Results

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MS data file    : cw0423_q2.pkl
Database        : Ashbya Ashbya (4796 sequences; 2346673 residues)
Timestamp       : 27 Jul 2007 at 14:22:06 GMT
Significant hits: mAEL111C  Pir protein,Syntenic homolog of Saccharomyces cerevisiae YKL163W (PIR3) and YJL160C; 
                  ABR025C   440619:441236,reverse,Syntenic homolog of Saccharomyces cerevisiae YKL096W (CWP1); Tandem gene quad
                  mAEL103W  Pir protein,Syntenic homolog of Saccharomyces cerevisiae YJL158C (CIS3), YKL164C (PIR1) and YKL163W

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 37 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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        Error tolerant   

1.    mAEL111C                          Mass: 33442    Score: 515    Queries matched: 11
 Pir protein,Syntenic homolog of Saccharomyces cerevisiae YKL163W (PIR3) and YJL160C;
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   407.22   812.43   812.49   -0.05 0  44  0.01 1  R.IGAIVANR.Q
5   502.74   1003.46   1003.48   -0.02 0  44  0.013 1  K.DPVTAVSCR.S
6   511.74   1021.46   1021.48   -0.02 0  36  0.073 1  K.DGVLYDGAGR.I
7   516.77   1031.53   1031.53   -0.01 0  11  28 1  K.LQAIDLVTC.-
10   545.80   1089.58   1089.59   -0.01 0  79  4.2e-006 1  R.SEGTLELTLK.D
18   491.22   1470.65   1470.69   -0.04 1  45  0.0088 1  K.HDSKDPVTAVSCR.S
19   736.35   1470.69   1470.69   0.00 1  (16) 7.6 1  K.HDSKDPVTAVSCR.S
21   824.40   1646.79   1646.80   -0.01 0  47  0.0049 1  N.LYDENIAPQCSPIK.L
24   881.41   1760.80   1760.84   -0.04 0  37  0.059 1  Y.NLYDENIAPQCSPIK.L
30   917.93   1833.84   1833.90   -0.07 0  121  2e-010 1  R.QFQFDGPPPQAGAIYAK.G
36   1151.94   2301.87   2302.06   -0.19 0  61  0.00026 1  L.SGSFYNLYDENIAPQCSPIK.L

 
      Proteins matching the same set of peptides:
      AEL111C                           Mass: 35909    Score: 513    Queries matched: 11
 415421:416467,reverse,Syntenic homolog of Saccharomyces cerevisiae YKL163W (PIR3) and YJL160C; Inve

2.    ABR025C                           Mass: 20232    Score: 265    Queries matched: 4
 440619:441236,reverse,Syntenic homolog of Saccharomyces cerevisiae YKL096W (CWP1); Tandem gene quad
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   462.71   923.41   923.43   -0.03 0  53  0.0012 1  K.GAFTAGSADK.A
9   531.75   1061.48   1061.48   0.00 0  23  1.4 1  D.DFEPAEGAAR.S
22   838.90   1675.79   1675.83   -0.04 0  122  1.8e-010 1  K.LGGSTADALSAVVTDDGK.L
34   987.89   1973.76   1973.85   -0.09 0  67  7.9e-005 1  R.SASMYHMSSVFEDNGALK.L + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      mABR025C                          Mass: 18217    Score: 265    Queries matched: 4
 hom. to Cwp1p,Syntenic homolog of Saccharomyces cerevisiae YKL096W (CWP1);
3.    mAEL103W                          Mass: 18618    Score: 81     Queries matched: 2
 Pir protein,Syntenic homolog of Saccharomyces cerevisiae YJL158C (CIS3), YKL164C (PIR1) and YKL163W
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 21   824.40   1646.79   1646.80   -0.01 0  47  0.0049 1  N.LYDQNIAPQCSPIK.L + Deamidation (NQ)
 24   881.41   1760.80   1760.84   -0.04 0  37  0.059 1  Y.NLYDQNIAPQCSPIK.L + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      AEL103W                           Mass: 18618    Score: 80     Queries matched: 2
 Pir protein,Syntenic homolog of Saccharomyces cerevisiae YJL158C (CIS3), YKL164C (PIR1) and YKL163W
4.    AFL121W                           Mass: 94569    Score: 33     Queries matched: 1
 209373:211949,forward,Non-syntenic homolog of Saccharomyces cerevisiae YBL022C
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   407.22   812.43   812.52   -0.09 1  36  0.052 2  Q.LGAIVRGK.I

5.    ADR112W                           Mass: 66717    Score: 33     Queries matched: 1
 903386:905119,forward,Syntenic homolog of Saccharomyces cerevisiae YDR484W (SAC2)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 21   824.40   1646.79   1646.82   -0.03 1  35  0.088 3  S.YLNDKLAIYDQYK.N + Deamidation (NQ)

6.    ADR359W                           Mass: 48132    Score: 32     Queries matched: 1
 1341916:1343187,forward,Syntenic homolog of Saccharomyces cerevisiae YOR334W (MRS2)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   545.80   1089.58   1089.56   0.03 0  32  0.18 2  Q.QSGSLLQNIK.S + 3 Deamidation (NQ)

7.    ABR161C                           Mass: 44223    Score: 27     Queries matched: 1
 705053:706273,reverse,Syntenic homolog of Saccharomyces cerevisiae YJR105W (ADO1)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   407.22   812.43   812.49   -0.05 0  27  0.47 3  F.VAALVQGR.D

8.    ACL006W                           Mass: 81389    Score: 23     Queries matched: 1
 344395:346521,forward,Syntenic homolog of Saccharomyces cerevisiae YMR001C (CDC5)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 24   881.41   1760.80   1760.68   0.12 0  24  0.95 3  K.NSNNNNNQINQNNEQ.L + 3 Deamidation (NQ)

9.    AGR066W                           Mass: 175574   Score: 21     Queries matched: 1
 837187:841920,forward,Syntenic homolog of Saccharomyces cerevisiae YDR127W (ARO1)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 30   917.93   1833.84   1833.92   -0.08 1  25  0.83 2  L.HSRLGATLDGNEPLPEK.E + Deamidation (NQ)

10.   ABR028C                           Mass: 38958    Score: 20     Queries matched: 2
 448648:449808,reverse,Syntenic homolog of Saccharomyces cerevisiae YKL096W (CWP1); Tandem gene quad
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
16   679.81   1357.60   1357.68   -0.09 1  16  6.7 1  R.TALGTALGSQPARD.L + Deamidation (NQ)
 21   824.40   1646.79   1646.84   -0.05 1  5  83 8  R.DLQSVKTGASQALQVT.A + 2 Deamidation (NQ)

11.   ABL205C                           Mass: 49226    Score: 19     Queries matched: 1
 22832:24187,reverse,Syntenic homolog of Saccharomyces cerevisiae YNL240C (NAR1)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   407.22   812.43   812.46   -0.03 1  19  2.8 4  G.LGPSQRR.D

12.   AFR412C                           Mass: 49524    Score: 19     Queries matched: 1
 1173600:1174919,reverse,Syntenic homolog of Saccharomyces cerevisiae YHR011W (DIA4)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   407.22   812.43   812.49   -0.05 0  19  2.9 5  P.LAGLGLNR.T

13.   AER057W                           Mass: 34482    Score: 19     Queries matched: 1
 741957:742895,forward,Syntenic homolog of Saccharomyces cerevisiae YDL065C (PEX19)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   462.71   923.41   923.43   -0.03 1  20  2.5 2  K.SGKFGAEQT.G

14.   ABR112C                           Mass: 246137   Score: 18     Queries matched: 2
 594245:600670,reverse,Syntenic homolog of Saccharomyces cerevisiae YCR093W (CDC39)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 29   909.42   1816.83   1816.73   0.09 0  11  19 3  R.QQQQQQQQQQQQQQ.Q + 6 Deamidation (NQ)
 30   917.93   1833.84   1834.02   -0.18 1  14  9.5 3  I.NAIVLHVGIEAALEKQK.T + 2 Deamidation (NQ)

15.   AAR129C                           Mass: 41332    Score: 16     Queries matched: 1
 572293:573420,reverse,Syntenic homolog of Saccharomyces cerevisiae YDR415C
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
29   909.42   1816.83   1816.79   0.04 0  17  5 1  L.QFNETYIADVTEEEK.L + 2 Deamidation (NQ)

16.   AGR099C                           Mass: 34550    Score: 16     Queries matched: 1
 924372:925289,reverse,Syntenic homolog of Saccharomyces cerevisiae YDR106W (ARP10)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   407.22   812.43   812.54   -0.10 1  16  6.4 6  R.LKIVVLE.N

17.   AEL148W                           Mass: 65622    Score: 15     Queries matched: 1
 351089:352810,forward,Syntenic homolog of Saccharomyces cerevisiae YJL186W (MNN5)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   545.80   1089.58   1089.56   0.03 0  15  9.2 3  S.SESGQLLIDK.A + Deamidation (NQ)

18.   ADL347C                           Mass: 17914    Score: 15     Queries matched: 1
 94934:95410,reverse,Syntenic homolog of Saccharomyces cerevisiae YML098W (TAF19)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   462.71   923.41   923.40   0.01 0  18  4.1 3  K.QFDNSEGK.S

19.   ACL115W                           Mass: 19397    Score: 15     Queries matched: 1
 143560:144075,forward,NOHBY307; No homolog in Saccharomyces cerevisiae
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   511.74   1021.46   1021.58   -0.12 1  16  6.9 2  L.TAARTHLPR.T

20.   ADR083W                           Mass: 57782    Score: 14     Queries matched: 1
 855232:856821,forward,Syntenic homolog of Saccharomyces cerevisiae YDL143W (CCT4)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   545.80   1089.58   1089.54   0.04 0  14  12 4  E.SGETNAGISVR.R

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
32   970.93   1939.85   1939.74   0.12 1  13  14 1  SSAGGASNGSRGNSDNSANNN + 3 Deamidation (NQ)
38   1306.00   2609.98   2609.20   0.78 1  13  18 1  GIMSSGGSSNYTGIHSTPAALTSREQ + Deamidation (NQ)
3   435.75   869.50   869.49   0.01 0  12  14 1  VVEASVGIP
37   1288.42   2574.82   2574.36   0.47 1  12  22 1  IILSEIFPQKLAMFDHIDAFVQ
28   897.89   1793.77   1793.84   -0.07 0  12  17 1  EPSEDSFLLLDCLEK
15   637.82   1273.64   1273.59   0.04 0  11  21 1  DHLGSYAPNTAK + Deamidation (NQ)
11   581.28   1160.55   1160.66   -0.10 0  11  23 1  INQVFLNVSK
20   795.85   1589.69   1589.83   -0.14 0  11  24 1  AVLETMQLINSTSAI
40   1467.98   2933.94   2933.49   0.45 1  8  47 1  AVDLLLEVESIDKLPQFIDENTFQR + 2 Deamidation (NQ)
17   703.29   1404.56   1403.79   0.77 1  8  53 1  NSRLPVPSLLAHT
14   425.53   1273.56   1273.65   -0.09 1  8  53 1  DDVETLLRDAK
12   584.28   1166.55   1166.68   -0.13 0  7  50 1  EVLLPLLEEL
31   965.39   1928.76   1928.07   0.69 0  7  73 1  SVIVSNIPVEVSNLTVMK
33   973.89   1945.77   1945.95   -0.18 0  5  1e+002 1  FLHAPNQAVLNGQEAPPAA + 2 Deamidation (NQ)
39   1389.35   2776.69   2776.42   0.27 1  4  1.4e+002 1  KVIEIPLYDYSVFYSLWLQCIQ
1   365.66   729.30   729.29   0.00 1  4  1.5e+002 1  KEDSNH + Deamidation (NQ)
25   884.38   1766.74   1766.78   -0.04 0  3  1.3e+002 1  LDGFQSNQNTAAEVSAI + 3 Deamidation (NQ)
23   841.85   1681.68   1681.86   -0.18 1  3  1.6e+002 1  RNPQLLGDLGAEELR + 2 Deamidation (NQ)
26   892.89   1783.76   1783.85   -0.09 0  2  1.6e+002 1  PCVLFFDEFDSIAPK
8   517.74   1033.47   1033.52   -0.05 1  2  1.7e+002 1  FRGQISEPT
13   636.30   1270.58   1270.61   -0.03 0  2  1.9e+002 1  FGEVDAITYQK + Deamidation (NQ)
35   1032.93   2063.84   2063.11   0.72 0  1  2.3e+002 1  NTDQIQAVIDANLIPPLVK + 2 Deamidation (NQ)
27   595.61   1783.79        
41   1299.91   3896.70        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : semiTrypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Deamidation (NQ)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.8 Da
Fragment Mass Tolerance: ± 0.4 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 41

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 15 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.6    0.00        K.KEDSNH.D
  2.6   -0.16        S.FVVPIR.F
  2.6   -0.16        V.FVVPLR.N
  0.2   -0.06        K.LEAATPE.P
  0.2   -0.09        K.LTVATPE.T
  0.2   -0.07        K.QGAQVVQ.T
  0.2   -0.06        K.SPLSPTQ.N
  0.2   -0.09        K.TAVIPTQ.F
  0.2   -0.09        K.VVVSPTQ.R
Top scoring peptide matches to query 2
Score greater than 36 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 43.6   -0.05    1  mAEL111C R.IGAIVANR.Q
 36.5   -0.09    4  AFL121W  Q.LGAIVRGK.I
 26.9   -0.05    7  ABR161C  F.VAALVQGR.D
 19.1   -0.03   11  ABL205C  G.LGPSQRR.D
 19.0   -0.05   12  AFR412C  P.LAGLGLNR.T
 15.6   -0.09                K.IKLVPLM.K
 15.6   -0.10   16  AGR099C  R.LKIVVLE.N
 13.6   -0.05                S.LQLGNLR.Q
 13.5   -0.06                IQLRQR
 13.5   -0.06                M.LQLRQR.L
Top scoring peptide matches to query 3
Score greater than 23 indicates homology
Score greater than 36 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 12.4    0.01        K.VVEASVGIP.T
 11.8   -0.00        R.VGDLLRTP.R
  9.8    0.02        E.VEVRDPR.T
  7.7    0.07        K.VGNTTIQH.R
  6.1    0.07        R.VATAEHVGS.A
  6.0    0.01        A.RQNSLPR.I
  5.8   -0.04        F.VGQLLALR.N
  5.1   -0.06        K.VKLALDLV.T
  4.7    0.03        I.LDVPSSPR.A
  4.7    0.11        S.PDDPSSPR.I
Top scoring peptide matches to query 4
Score greater than 36 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 53.2   -0.03    2  ABR025C K.GAFTAGSADK.A
 20.1   -0.03   13  AER057W K.SGKFGAEQT.G
 17.9    0.01   18  ADL347C K.QFDNSEGK.S
 16.7    0.01               G.FQTGNNDK.D
 16.6   -0.03               Y.SGKFGADDK.D
 11.8   -0.05               D.FIESATEK.A
 11.4   -0.14               V.PPKPKTEK.A
 11.2   -0.03               K.NDFAKSAAT.I
 11.0    0.01               G.FQTGNNDK.D
 10.7   -0.03               S.KSSVSCQK.H
Top scoring peptide matches to query 5
Score greater than 26 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 43.9   -0.02    1  mAEL111C K.DPVTAVSCR.S
 10.5   -0.09                V.LVSLVASCR.I
  8.5    0.05                K.PDEAAACNR.L
  7.4    0.01                P.VLVNDNNGGT.-
  7.4    0.01                P.VLVNDNNGGT.-
  6.8   -0.06                K.DVHNIIHR.D
  6.6   -0.09                C.VLEAARSMK.D
  5.9    0.04                R.NYMPEGHR.L
  2.5   -0.10                Y.VLASLTANSK.R
  1.5   -0.10                R.IVVEGKTTGT.T
Top scoring peptide matches to query 6
Score greater than 31 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 35.9   -0.02    1  mAEL111C K.DGVLYDGAGR.I
 16.1   -0.12   19  ACL115W  L.TAARTHLPR.T
 10.1    0.03                R.ATMQDNLQV.Q
  9.4   -0.02                R.NGVLTFHFS.P
  8.2   -0.05                K.IVFDQGTGVS.K
  8.1   -0.09                R.RSFTALQLS.S
  7.7    0.00                L.RNLTCNSR.K
  5.0    0.02                K.AISLESQCD.S
  4.3    0.01                W.TAVDGSMQGR.D
  4.2    0.01                S.RNLESNCK.R
Top scoring peptide matches to query 7
Score greater than 21 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 10.6   -0.01    1  mAEL111C K.LQAIDLVTC.-
  7.9   -0.04                P.QIANMIITK.A
  7.3   -0.02                K.ELIGKIESGS.S
  6.1    0.03                K.VAAADLVNCV.I
  5.3    0.04                R.TVRALANDGD.G
  3.9    0.04                K.ISRAINDED.D
  3.8    0.09                R.TGATLNDPQD.D
  3.6    0.01                K.LQGKLVDGDS.P
  3.6   -0.02                R.LNALKTANSV.A
  3.5   -0.03                Q.KLANLSQTR.F
Top scoring peptide matches to query 8
Score greater than 15 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.4   -0.05        R.FRGQISEPT.I
  2.4   -0.08        K.NFVGAAETII.N
  2.1    0.06        R.DCPNGQLTQ.E
  1.6   -0.04        R.TDERGLSSAV.L
  1.5   -0.06        R.AVLFPCIAGS.M
  1.3   -0.04        R.NSSGISLPPY.S
  1.3    0.01        R.TQDISNLDQ.A
  1.3    0.01        K.TQDLSIGGQN.F
  1.3    0.01        K.TQDLSIGGQN.F
  1.3   -0.02        R.SCIIGSANVN.E
Top scoring peptide matches to query 9
Score greater than 30 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 23.4    0.00    2  ABR025C D.DFEPAEGAAR.S
 16.0   -0.11               T.VYEAIIAQR.N
  4.5    0.11               K.DDIDDPEDQ.G
  4.4   -0.05               W.FDEHFVLR.R
  3.4    0.02               K.NNPLTEQLM.H
  3.1   -0.10               A.FDEKLPVSK.T
  3.1    0.02               R.ILWQEEEN.S
  3.1   -0.02               R.LLGQQQQLM.H
  2.9    0.03               R.QQNSIPQTSG.D
  2.7    0.02               K.ARGDISAEED.V
Top scoring peptide matches to query 10
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 78.7   -0.01    1  mAEL111C R.SEGTLELTLK.D
 32.4    0.03    6  ADR359W  Q.QSGSLLQNIK.S
 15.3    0.03   17  AEL148W  S.SESGQLLIDK.A
 14.2    0.04   20  ADR083W  E.SGETNAGISVR.R
 12.8    0.02                N.DTSKNGLDLK.T
 12.7    0.01                N.PDKFELVDK.L
 12.1   -0.02                G.TNGIGKSTALK.I
 12.1    0.03                F.ENDKETLLK.R
 12.1   -0.02                D.ILNFEDVIK.G
 11.5    0.10                K.ADSSLQDPEK.S
Top scoring peptide matches to query 11
Score greater than 15 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 11.2   -0.10        Q.INQVFLNVSK.G
  2.3   -0.01        R.SNLTSKAAESGP.A
  2.1    0.05        K.SQDEAAQANRA.V
  1.5   -0.05        E.VYIQQVNPAK.T
  1.0   -0.02        R.SSTRVVPTNSN.N
  0.9   -0.03        P.CTQGPGKVVSK.S
Top scoring peptide matches to query 12
Score greater than 17 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  7.3   -0.13        K.EVLLPLLEEL.A
  7.3   -0.02        L.HSDDPLASLGR.L
  7.3   -0.13        D.LAAAAGLPASAVR.L
  7.3   -0.04        P.VPEQLPAEQR.R
  3.8   -0.03        Q.AVHNGGELSQR.R
  3.8   -0.14        M.GIFIPHSKLR.N
  2.5    0.12        K.MSYETTCQF.L
  1.1   -0.08        A.GLFHPARNVR.K
Top scoring peptide matches to query 13
Score greater than 14 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.8   -0.03        Y.FGEVDAITYQK.Y
  1.7    0.03        K.YLMTQTNGSQQ.P
Top scoring peptide matches to query 14
Score greater than 19 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  7.6   -0.09        Y.DDVETLLRDAK.R
  6.5   -0.22        D.AVKFLNGLITAK.L
  4.5    0.00        K.QAQSESNTGPGAQ.G
  4.1   -0.08        E.LSNQWNKADAK.I
  3.9   -0.16        E.KLYHMGILATK.S
  2.8   -0.04        D.DHLGSYAPNTAK.K
  2.5   -0.07        R.SRLEQAQQDAK.K
  2.0   -0.09        K.EKATDDGLVQAK.K
  1.5   -0.15        E.LAALVNFLMPGK.F
Top scoring peptide matches to query 15
Score greater than 21 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 11.4    0.04        D.DHLGSYAPNTAK.K
 10.9   -0.07        L.LVNFGALGGEVAK.N
  7.6    0.02        A.NVDNSIANDIAK.F
  6.3    0.08        K.QAQSESNTGPGAQ.G
  5.8   -0.02        R.EANLNSLVRVTG.V
  2.7    0.02        A.NVDNSIANDIAK.F
  2.5    0.00        R.AGATGVAHTLFTE.Q
  2.2    0.03        K.LQTEEYSYKL.C
  2.1    0.03        M.AGNESLIQEIAAG.L
  1.9    0.03        K.TDEQVEQILGAA.K
Top scoring peptide matches to query 16
Score greater than 22 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 16.4   -0.09   10  ABR028C R.TALGTALGSQPARD.L
  8.8    0.00               K.EQAGGGAGGGAGGAEGR.P
  8.3   -0.13               E.RQAASLGQSAQLK.L
  6.1    0.80               N.REITSSLLLTPK.D
  5.4   -0.08               K.EGVAVNNDQLSVL.L
  5.0   -0.15               K.WLQDLMLKPSK.M
  4.2   -0.06               K.FFYLGGTEEAPK.N
  2.2   -0.17               D.TIENILIVDISK.S
  1.8   -0.17               A.ILLEMLGNNITK.I
  1.4    0.77               L.DLASALLLLSTIK.W
Top scoring peptide matches to query 17
Score greater than 19 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  7.8    0.77        R.NSRLPVPSLLAHT.L
  6.2    0.71        R.VQLRAPAARPGLR.H
  3.1   -0.13        K.FIQYIVLQQHD.N
  3.1    0.79        R.RLYAHVFLSWI.F
  3.1   -0.09        R.QQTEIYGSKVHD.E
  3.1   -0.09        R.QQTEIYGSKVHD.E
  2.7   -0.11        K.LKVYIESCAANH.Q
  2.5   -0.17        R.NIGVAPSSQLPQPP.P
  2.4   -0.20        R.KHIPQNILTTAAP.S
  2.3    0.73        P.RLSLAPPPINIGR.R
Top scoring peptide matches to query 18
Score greater than 22 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 45.1   -0.04    1  mAEL111C K.HDSKDPVTAVSCR.S
  6.8   -0.13                K.PNPSADPVFSKAIK.V
  1.3    0.00                N.GGYTFGPFDPEQR.L
Top scoring peptide matches to query 19
Score greater than 16 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 15.6    0.00    1  mAEL111C K.HDSKDPVTAVSCR.S
  2.9    0.10                R.YMDRLSNSYYC.F
  1.6    0.06                S.PVVQGYENEFCK.E
  1.4   -0.08                D.PTLRDVGGADQITK.V
  1.2   -0.12                E.TLEQFLRLPPEK.I
  0.9   -0.03                Y.SPPLSTLPSNNNTK.C
  0.9   -0.03                Y.SPPLSTLPSNNNTK.C
  0.9   -0.03                Y.SPPLSTLPSNNNTK.C
  0.9    0.02                F.ASHATNDANRVESV.-
  0.9   -0.02                D.PLGSLNDAHYNLR.I
Top scoring peptide matches to query 20
Score greater than 14 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 10.6   -0.14        K.AVLETMQLINSTSAI.H
 10.6    0.76        L.GIMVSREALLLFLK.S
 10.6   -0.00        R.GINMQNLEQEREQ.L
 10.6   -0.00        R.GINMQNLEQEREQ.L
 10.6   -0.00        R.GINMQNLEQEREQ.L
 10.6   -0.15        S.VAFQGVGISLVNTER.L
  0.7   -0.17        A.VAVVVLVTAANDYQK.E
Top scoring peptide matches to query 21
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 47.3   -0.01    1  mAEL111C N.LYDENIAPQCSPIK.L
 47.3   -0.01    3  mAEL103W N.LYDQNIAPQCSPIK.L
 34.8   -0.01    3  mAEL103W N.LYDQNIAPQCSPIK.L
 34.8   -0.03    5  ADR112W  S.YLNDKLAIYDQYK.N
 11.6   -0.06                K.FERMALLDRPVAET.P
 11.5   -0.10                S.DFQRSLDAVLLLEK.S
  5.8   -0.00                R.DQQTTRVNALQMEI.L
  5.0   -0.05   10  ABR028C  R.DLQSVKTGASQALQVT.A
  4.9    0.04                V.ENFQQVLGNFYTGK.D
  4.5   -0.01    3  mAEL103W N.LYDQNIAPQCSPIK.L
Top scoring peptide matches to query 22
Score greater than 32 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
121.6   -0.04    2  ABR025C K.LGGSTADALSAVVTDDGK.L
 12.2   -0.03               L.RSAGNWSLGLQETEK.S
  7.7   -0.06               K.KIEQYINIEKPNW.K
  7.2   -0.10               R.AGHVSVLLNLAPKDADG.D
  6.7   -0.10               A.ENEVYGAKLWAGLVK.E
  6.1   -0.07               R.FAQVIDESLAVLQQL.S
  5.7   -0.09               C.SSGSDISVGIVLAALCK.N
  5.4    0.04               K.TANAEQAVKAEEQADT.G
  3.0   -0.05               K.TSPQNLASFGALTAALN.Y
  2.4   -0.05               K.TSPQNLASFGALTAALN.Y
Top scoring peptide matches to query 23
Score greater than 15 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.9   -0.18        E.RNPQLLGDLGAEELR.A
  2.6    0.80        K.DFHPVSVLIPSQSVR.G
  2.4    0.69        K.FVLVNTLQQKLHLK.C
  2.4    0.69        K.FVLVNTLQQKLHLK.C
  2.4   -0.30        K.FVLVNTLQQKLHLK.C
  0.7   -0.14        -.MDNARQAGAHQSIVLA.A
  0.6    0.75        H.PAVMSPLAKSAGLVANR.F
  0.5   -0.06        R.NCGHTYYIKNSIPN.L
  0.1   -0.26        R.DTLKPAGVNIERVVAT.L
  0.0   -0.18        E.EAAALLRNGIDPADEK.D
Top scoring peptide matches to query 24
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 36.6   -0.04    1  mAEL111C Y.NLYDENIAPQCSPIK.L
 36.6   -0.04    3  mAEL103W Y.NLYDQNIAPQCSPIK.L
 24.5    0.12    8  ACL006W  K.NSNNNNNQINQNNEQ.L
 23.4   -0.04    3  mAEL103W Y.NLYDQNIAPQCSPIK.L
 22.8   -0.04    3  mAEL103W Y.NLYDQNIAPQCSPIK.L
  9.1   -0.03                N.PLDNPLLTYVQDDEK.T
  7.7   -0.08                A.LSKPEGHFRATFEDK.I
  7.6   -0.01                M.LPNIYPESAAAEDENK.Y
  7.5   -0.05                F.NYLLMAYSRYQPNK.L
  7.0    0.79                E.IPPTKPARPFGLQNPK.R
Top scoring peptide matches to query 25
Score greater than 15 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.2   -0.04        R.LDGFQSNQNTAAEVSAI.I
  2.6    0.80        K.RIDTLLYLMIYPQQ.P
  2.5   -0.00        K.QQFDQMRANYSHQN.T
  2.5   -0.00        K.QQFDQMRANYSHQN.T
  2.2   -0.04        E.FCDIINSGEAENEIR.L
  1.5   -0.21        Q.LKVQFQTGDTLQFIK.L
  1.5   -0.21        Q.LKVQFQTGDTLQFIK.L
  1.5   -0.05        R.FNFRSPQQINDDAAGS.H
  1.5   -0.05        R.FNFRSPQQINDDAAGS.H
  1.5   -0.05        R.FNFRSPQQINDDAAGS.H
Top scoring peptide matches to query 26
Score greater than 14 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.5   -0.09        K.PCVLFFDEFDSIAPK.R
  2.4    0.78        K.ILNPNLEPEVGAPHKR.I
  1.4   -0.07        Q.IGSYVQAFFDSMGHPK.Y
  0.9    0.00        K.QKQQQFFANAEPSNSG.K
  0.9    0.00        K.QKQQQFFANAEPSNSG.K
  0.8   -0.16        L.GSIPLNESTVTEALPQK.F
  0.5   -0.15        L.KQVQGHGILVGPNYSGK.T
Top scoring peptide matches to query 27
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 28
Score greater than 23 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 11.9   -0.07        Y.EPSEDSFLLLDCLEK.E
 10.2   -0.01        L.EDNSDKFALHEGFER.Y
  9.6    0.01        K.FLQQQQQLMQSGPQQ.T
  9.5   -0.18        D.PKKPIGGNIMAHSSTTR.L
  9.2   -0.14        S.YHTASSKGVSILSQSTK.L
  9.0    0.05        R.MQEGQSPTGTSVNVEND.S
  8.7   -0.18        R.QFFIPLTGISVNSTLR.E
  7.9   -0.20        K.IQLPPPSRLFNVPSTQ.-
  7.4   -0.12        K.LVSFQNSGVSVEDRTR.T
  6.5   -0.12        K.LVSFQNSGVSVEDRTR.T
Top scoring peptide matches to query 29
Score greater than 25 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 17.2    0.04   15  AAR129C L.QFNETYIADVTEEEK.L
 11.7   -0.00               K.EQQRLQDNLQGLDISG.G
 11.4    0.09   14  ABR112C R.QQQQQQQQQQQQQQ.Q
  8.1   -0.14               I.NGKSFNALLQLPPAAFK.L
  6.9    0.08               R.FDSDLQEVVYNACEQ.R
  6.3   -0.13               K.TGSLYAIVLYDFKAEK.S
  6.2    0.03               R.QQKQQQQQQQLSQSP.Q
  6.1    0.02               P.QEKQQQQQNEQLQR.I
  6.1    0.03               R.QQKQQQQQQQLSQSP.Q
  5.6    0.09   14  ABR112C R.QQQQQQQQQQQQQQ.Q
Top scoring peptide matches to query 30
Score greater than 36 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
120.8   -0.07    1  mAEL111C R.QFQFDGPPPQAGAIYAK.G
 24.7   -0.08    9  AGR066W  L.HSRLGATLDGNEPLPEK.E
 14.2   -0.18   14  ABR112C  I.NAIVLHVGIEAALEKQK.T
 10.2    0.12                L.QCWSSNGHMNPVCEK.L
 10.1   -0.12                S.IPNSVGPIQKWSLHEK.W
  9.0   -0.10                G.QSISQAVLTVPTNFAEK.Q
  8.7    0.02                R.WSIAMSLPDWCPPFQ.G
  8.1   -0.12                V.QFWNQLNLKSAATLAK.H
  8.1   -0.12                V.QFWNQLNLKSAATLAK.H
  7.4   -0.08                P.KHLDEVTVTNAVDHEK.D
Top scoring peptide matches to query 31
Score greater than 19 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  6.7    0.69        H.SVIVSNIPVEVSNLTVMK.F
  6.7    0.76        S.YNSIIRGCPGVPQTLPR.I
  6.5   -0.10        K.LLMEGSYQKAWDMLQN.G
  5.8   -0.17        E.LPDGPQNMQAKVYNPEK.K
  4.3   -0.22        N.AIDLFVTNQVPTEEPEK.S
  3.5   -0.26        A.WWNLLNKYHIVLDSK.F
  3.3   -0.17        R.DIQDNPQNADPRLLYAS.L
  3.3    0.78        S.VCYVTVVLQGTAFTQDK.T
  3.3   -0.20        S.VCYVTVVLQGTAFTQDK.T
  2.8    0.00        R.QNPSMMRNMMGMLGGGSGA.A
Top scoring peptide matches to query 32
Score greater than 22 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 12.7    0.12        K.SSAGGASNGSRGNSDNSANNN.S
  8.3   -0.05        K.FTQEEQDAIVNYVAPSK.E
  2.2   -0.04        N.IQDKSTNHHNMANSIVK.H
  1.5   -0.12        R.YDNQIAVFGLDFQRALA.N
  1.3   -0.24        -.MRIAATFSLLFLAIFQV.S
  0.5   -0.06        R.SGRILFGFFHPFCNAGGG.G
  0.0   -0.00        Y.MSSSDENTPLVKDHDHK.H
Top scoring peptide matches to query 33
Score greater than 16 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  5.1   -0.18        K.FLHAPNQAVLNGQEAPPAA.P
  2.9   -0.24        K.QLLLLSLPADARPAQHSD.M
  2.7    0.74        K.QLLLLSLPADARPAQHSD.M
  1.8   -0.32        N.PPVFKLQIGSPDQPPVVK.L
  1.7   -0.17        T.EQALLHTIQQNFSSNSK.T
  1.7   -0.17        T.EQALLHTIQQNFSSNSK.T
  1.7   -0.17        T.EQALLHTIQQNFSSNSK.T
  1.1    0.67        N.PPVFKLQIGSPDQPPVVK.L
  0.5   -0.03        K.LSEYTQGWGNMASTATDD.L
Top scoring peptide matches to query 34
Score greater than 25 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 66.5   -0.09    2  ABR025C R.SASMYHMSSVFEDNGALK.L
  8.0    0.76               Q.DQLFQFLQGHNIRLNK.V
  7.0    0.79               P.AYMQMVLDQSRQLYVK.R
  5.8    0.77               M.VGEFNELLQLPEDELVK.A
  5.6    0.77               R.TGSGANNAPLGKPRQSAAFK.G
  5.6   -0.22               R.TGSGANNAPLGKPRQSAAFK.G
  5.3    0.79               E.ENQQIWDREALQTLVK.Q
  5.2   -0.08               T.MNMNTDFRASSQTDDLK.Y
  4.4   -0.30               E.LLIVTLNQSSLDIDMAVK.I
  4.3    0.74               L.TATLNNTGFNFIRYIVK.N
Top scoring peptide matches to query 35
Score greater than 14 indicates homology
Score greater than 37 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.2    0.72        G.NTDQIQAVIDANLIPPLVK.L
  1.2    0.72        G.NTDQIQAVIDANLIPPLVK.L
  0.8    0.72        G.NTDQIQAVIDANLIPPLVK.L
  0.5   -0.26        G.NTDQIQAVIDANLIPPLVK.L
  0.5   -0.26        G.NTDQIQAVIDANLIPPLVK.L
  0.1   -0.26        G.NTDQIQAVIDANLIPPLVK.L
Top scoring peptide matches to query 36
Score greater than 24 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 61.3   -0.19    1  mAEL111C L.SGSFYNLYDENIAPQCSPIK.L
  7.9    0.72                E.DGLSIIETAQQVNVFVQDPAR.V
  7.9    0.72                E.DGLSIIETAQQVNVFVQDPAR.V
  4.9   -0.27                E.DGLSIIETAQQVNVFVQDPAR.V
  4.9   -0.27                E.DGLSIIETAQQVNVFVQDPAR.V
  4.2    0.72                E.DGLSIIETAQQVNVFVQDPAR.V
  4.0    0.72                E.DGLSIIETAQQVNVFVQDPAR.V
  4.0    0.72                E.DGLSIIETAQQVNVFVQDPAR.V
  3.1   -0.19                F.AGDQRLSGEVNEDTTPDLQQK.Y
  2.9    0.76                K.ALQISNQGSSSTGAAVGAAGEAAVPS.M
Top scoring peptide matches to query 37
Score greater than 25 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 12.3    0.47        R.IILSEIFPQKLAMFDHIDAFVQ.V
 12.3   -0.51        R.IILSEIFPQKLAMFDHIDAFVQ.V
Top scoring peptide matches to query 38
Score greater than 23 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 12.6    0.78        R.GIMSSGGSSNYTGIHSTPAALTSREQ.T
 12.6    0.77        S.SMTLNTVHNNTVRSSDAAQYSVGR.Y
 12.6    0.77        S.SMTLNTVHNNTVRSSDAAQYSVGR.Y
 12.6   -0.22        S.SMTLNTVHNNTVRSSDAAQYSVGR.Y
 12.6    0.77        S.SMTLNTVHNNTVRSSDAAQYSVGR.Y
 12.6   -0.22        S.SMTLNTVHNNTVRSSDAAQYSVGR.Y
 12.6   -0.22        S.SMTLNTVHNNTVRSSDAAQYSVGR.Y
  9.9   -0.38        T.SDGFITFFSSVQLPRLHYLNLR.R
  9.6   -0.20        R.AVSVAYKTDESYTVDDAFTMLNNG.V
  8.5    0.77        R.EYQHHGSAATNNFLIKHISPSQQ.N
Top scoring peptide matches to query 39
Score greater than 15 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.6    0.27        R.KVIEIPLYDYSVFYSLWLQCIQ.D
  2.2    0.40        Q.TGTPNAEGNEDILEFLDELEQSNLK.V
  2.2    0.40        Q.TGTPNAEGNEDILEFLDELEQSNLK.V
Top scoring peptide matches to query 40
Score greater than 20 indicates homology
Score greater than 38 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  8.3    0.45        D.AVDLLLEVESIDKLPQFIDENTFQR.V
  8.3    0.45        D.AVDLLLEVESIDKLPQFIDENTFQR.V
  7.1    0.47        K.IKVQGSEALLTSLFDILADLDTLDNDGG.L
  7.1    0.45        R.KVIFATNIAETSITIDGIYYVVDPGYA.K
  7.1   -0.50        A.NARTWSVGYLLDQVVLQTSFEYTSR.T
  7.1    0.54        R.NLPELVPPYCNYLALQGYHEPKMTS.Y
  7.1   -0.45        R.NLPELVPPYCNYLALQGYHEPKMTS.Y
  7.1   -0.37        V.VFTRCGHPFCESCLLEYIQFQNK.K
  7.1   -0.37        V.VFTRCGHPFCESCLLEYIQFQNK.K
  7.1   -0.37        V.VFTRCGHPFCESCLLEYIQFQNK.K
Top scoring peptide matches to query 41
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide