Mascot Search Results

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MS data file    : cw0401_q3.pkl
Database        : spombe A_gossypii (4731 sequences; 2318540 residues)
Timestamp       : 27 Apr 2007 at 07:43:18 GMT
Significant hits: Q756G2|TOM1_ASHGO  Probable E3 ubiquitin protein ligase TOM1 (EC 6.3.2.-) - Ashbya gossypii (Yeast) (Eremothecium goss

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 25 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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1.    Q756G2|TOM1_ASHGO                 Mass: 373977   Score: 27     Queries matched: 1
 Probable E3 ubiquitin protein ligase TOM1 (EC 6.3.2.-) - Ashbya gossypii (Yeast) (Eremothecium goss
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   421.79   841.56   841.50   0.06 0  27  0.036 1  R.IGLSSIPR.L

2.    Q753X4|Q753X4_ASHGO               Mass: 89453    Score: 17     Queries matched: 1
 AFR198Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   523.81   1045.60   1045.46   0.14 0  17  0.51 1  K.NSSYFWSR.S

3.    Q755C8|PUS1_ASHGO                 Mass: 60157    Score: 11     Queries matched: 1
 tRNA pseudouridine synthase 1 (EC 5.4.99.-) (tRNA-uridine isomerase 1) (tRNA pseudouridylate syntha
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   971.02   1940.02   1939.91   0.11 0  11  1.5 1  K.FTQEEQDAIVNYVAPSK.E + 2 Deamidation (NQ)

4.    Q751I4|Q751I4_ASHGO               Mass: 26178    Score: 9      Queries matched: 1
 AGL278Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   421.79   841.56   841.47   0.10 0  9  2.5 2  R.GTLEAVPR.R

5.    Q750Q1|Q750Q1_ASHGO               Score: 7      Queries matched: 1
 AGL109Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   421.79   841.56   841.50   0.06 0  9  2.6 3  R.LTVSAVPR.E

6.    Q75C32|Q75C32_ASHGO               Score: 7      Queries matched: 1
 ACR085Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   897.95   1793.88   1793.96   -0.07 0  7  4 2  R.QFFIPLTGISVNSTLR.E + 2 Deamidation (NQ)

7.    Q75DV0|Q75DV0_ASHGO               Mass: 159064   Score: 7      Queries matched: 1
 ABL077Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   747.08   2238.23   2238.04   0.18 0  7  4.5 1  R.EQNVVFITSTGGQGEFPQDGK.A + Deamidation (NQ)

8.    Q756R6|Q756R6_ASHGO               Mass: 247767   Score: 5      Queries matched: 1
 AER188Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   897.95   1793.88   1793.89   -0.01 1  7  3.9 1  K.LVSFQNSGVSVEDRTR.T + Deamidation (NQ)

9.    Q754Y2|Q754Y2_ASHGO               Mass: 39430    Score: 4      Queries matched: 1
 AFL071Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   747.08   2238.23   2238.98   -0.75 0  4  7.4 3  R.LEDDSSLFPDTAAGEDPFADK.N

10.   Q75A83|MRD1_ASHGO                 Score: 3      Queries matched: 1
 Multiple RNA-binding domain-containing protein 1 - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   897.95   1793.88   1793.93   -0.04 1  3  9.2 3  K.QTKPQHGIDAEISKNK.Q + Deamidation (NQ)

11.   Q75A65|Q75A65_ASHGO               Score: 1      Queries matched: 1
 ADR053Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   421.79   841.56   841.50   0.06 0  1  14 4  K.AAIEILGR.Q

12.   Q750I7|Q750I7_ASHGO               Score: 1      Queries matched: 1
 AGL036Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   421.79   841.56   841.49   0.07 0  1  14 5  R.LTGIPVNK.L + Deamidation (NQ)

13.   Q755A7|RL7_ASHGO                  Score: 1      Queries matched: 1
 60S ribosomal protein L7 - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   971.02   1940.02   1940.16   -0.14 1  1  13 3  K.VLQLLRLNQINSGVFVK.V

14.   Q74ZK4|GSHR_ASHGO                 Score: 1      Queries matched: 1
 Glutathione reductase (EC 1.8.1.7) (GR) (GRase) - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   897.95   1793.88   1793.87   0.01 0  1  15 5  K.HYDYLVIGGGSGGVASSR.R

15.   Q75ER5|Q75ER5_ASHGO               Score: 0      Queries matched: 1
 AAR014Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   421.79   841.56   841.44   0.12 1  0  17 6  R.LGRENPR.G + Deamidation (NQ)

16.   Q75F27|Q75F27_ASHGO               Score: 0      Queries matched: 1
 AAL099Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   421.79   841.56   841.41   0.15 0  0  17 7  R.NMLPNPR.T + Deamidation (NQ)

17.   Q75ES2|Q75ES2_ASHGO               Score: 0      Queries matched: 1
 AAR007Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   421.79   841.56   841.54   0.03 0  0  19 8  K.VLAGLTIR.E

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   428.80   427.79        
2   435.81   434.80        
3   592.79   591.78        
8   1042.07   2082.13        
9   737.73   2210.18        
10   742.41   2224.22        
12   983.83   2948.48        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Deamidation (NQ),Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.8 Da
Fragment Mass Tolerance: ± 0.4 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 12

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 3 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 2
Score greater than 6 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 3
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 4
Score greater than 23 indicates homology
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein             Peptide
 27.3    0.06    1  Q756G2|TOM1_ASHGO   R.IGLSSIPR.L
  8.9    0.10    4  Q751I4|Q751I4_ASHGO R.GTLEAVPR.R
  8.7    0.06    5  Q750Q1|Q750Q1_ASHGO R.LTVSAVPR.E
  1.3    0.06   11  Q75A65|Q75A65_ASHGO K.AAIEILGR.Q
  1.3    0.07   12  Q750I7|Q750I7_ASHGO R.LTGIPVNK.L
  0.5    0.12   15  Q75ER5|Q75ER5_ASHGO R.LGRENPR.G
  0.5    0.15   16  Q75F27|Q75F27_ASHGO R.NMLPNPR.T
  0.1    0.03   17  Q75ES2|Q75ES2_ASHGO K.VLAGLTIR.E
Top scoring peptide matches to query 5
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein             Peptide
 16.5    0.14    2  Q753X4|Q753X4_ASHGO K.NSSYFWSR.S
Top scoring peptide matches to query 6
Score greater than 16 indicates homology
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein             Peptide
  6.6   -0.01    8  Q756R6|Q756R6_ASHGO K.LVSFQNSGVSVEDRTR.T
  6.6   -0.07    6  Q75C32|Q75C32_ASHGO R.QFFIPLTGISVNSTLR.E
  2.9   -0.04   10  Q75A83|MRD1_ASHGO   K.QTKPQHGIDAEISKNK.Q
  1.7   -0.01    8  Q756R6|Q756R6_ASHGO K.LVSFQNSGVSVEDRTR.T
  0.7    0.01   14  Q74ZK4|GSHR_ASHGO   K.HYDYLVIGGGSGGVASSR.R
Top scoring peptide matches to query 7
Score greater than 14 indicates homology
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein           Peptide
 10.8    0.11    3  Q755C8|PUS1_ASHGO K.FTQEEQDAIVNYVAPSK.E
 10.8    0.11    3  Q755C8|PUS1_ASHGO K.FTQEEQDAIVNYVAPSK.E
  1.2   -0.14   13  Q755A7|RL7_ASHGO  K.VLQLLRLNQINSGVFVK.V
Top scoring peptide matches to query 8
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 9
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 10
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 11
Score greater than 17 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein             Peptide
  6.5    0.18    7  Q75DV0|Q75DV0_ASHGO R.EQNVVFITSTGGQGEFPQDGK.A
  5.0    0.18    7  Q75DV0|Q75DV0_ASHGO R.EQNVVFITSTGGQGEFPQDGK.A
  4.4   -0.75    9  Q754Y2|Q754Y2_ASHGO R.LEDDSSLFPDTAAGEDPFADK.N
Top scoring peptide matches to query 12
Score greater than 24 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide