Mascot Search Results

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MS data file    : cw0400_q1.pkl
Database        : spombe A_gossypii (4731 sequences; 2318540 residues)
Timestamp       : 27 Apr 2007 at 07:40:39 GMT
Significant hits: Ssr1  AER058Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
                  Pir2  AEL111Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
                  Fig2  AFR711Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
                  Gas1  AGL352Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 26 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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        Error tolerant   

1.    Ssr1                              Mass: 19834    Score: 210    Queries matched: 5
 AER058Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   496.22   990.42   990.46   -0.04 0  14  0.85 1  D.VAHFCTKE.S
7   619.77   1237.53   1237.58   -0.04 0  53  9.9e-005 1  E.GALSAFKANCAE.H
13   830.32   1658.63   1658.67   -0.04 1  71  2.4e-006 1  E.SDAMQKCFSSICPE.G
17   581.25   1740.73   1740.79   -0.06 1  71  1.8e-006 1  E.SSHPLNDVAHFCTKE.S
18   871.38   1740.74   1740.79   -0.04 1  (65) 7.4e-006 1  E.SSHPLNDVAHFCTKE.S

 
      Proteins matching the same set of peptides:
      Q757F5|Q757F5_ASHGO               Mass: 22944    Score: 210    Queries matched: 5
 AER058Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)

2.    Pir2                              Mass: 33919    Score: 107    Queries matched: 2
 AEL111Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
20   714.35   2140.04   2140.10   -0.06 1  (1) 15 1  E.NIAPQCSPIKLQAIDLVTC.-
21   1071.04   2140.07   2140.10   -0.03 1  107  3.3e-010 1  E.NIAPQCSPIKLQAIDLVTC.-

 
      Proteins matching the same set of peptides:
      Q757X3|Q757X3_ASHGO               Mass: 35909    Score: 107    Queries matched: 2
 AEL111Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
3.    Fig2                              Mass: 55938    Score: 97     Queries matched: 3
 AFR711Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   545.25   1088.48   1088.52   -0.03 1  18  0.35 1  E.GLKVTAPDCE.A
5   608.28   1214.54   1214.58   -0.04 1  3  12 1  -.ATYNVKLDYE.Q
19   980.96   1959.90   1959.94   -0.04 1  76  4.3e-007 1  E.QYVDVFYHFKSGGVSVE.A

4.    Gas1                              Mass: 55771    Score: 42     Queries matched: 1
 AGL352Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   698.85   1395.68   1395.72   -0.04 0  42  0.0011 1  E.FSRLPVPAFFSE.Y

 
      Proteins matching the same set of peptides:
      Q751P1|Q751P1_ASHGO               Mass: 58032    Score: 42     Queries matched: 1
 AGL352Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
5.    Q751A2|Q751A2_ASHGO               Mass: 116428   Score: 19     Queries matched: 1
 AGL078Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   623.32   1244.63   1244.68   -0.05 0  19  0.27 1  D.IRSMAQLAILE.L + Deamidation (NQ)

6.    Q75AU0|Q75AU0_ASHGO               Mass: 62572    Score: 15     Queries matched: 1
 ADL170Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   623.32   1244.63   1244.65   -0.02 1  16  0.54 2  E.SVEQNKLALIE.K + 2 Deamidation (NQ)

7.    Q75CE2|Q75CE2_ASHGO               Mass: 95183    Score: 13     Queries matched: 1
 ACR128Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 19   980.96   1959.90   1959.79   0.11 1  13  0.96 2  E.GAYYNYHLNMINNNEE.I + 2 Deamidation (NQ)

8.    Ecm33                             Mass: 39065    Score: 10     Queries matched: 1
 ABL063Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   623.32   1244.63   1244.67   -0.04 1  10  2.2 3  D.SLKLMDLPSLE.A

 
      Proteins matching the same set of peptides:
      Q75DT6|Q75DT6_ASHGO               Mass: 42563    Score: 10     Queries matched: 1
 ABL063Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
9.    Q74ZH1|Q74ZH1_ASHGO               Score: 9      Queries matched: 1
 AGR236Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 19   980.96   1959.90   1959.91   -0.01 1  9  2.1 5  E.NSWLSDAGLVANILNQNE.G + 3 Deamidation (NQ)

10.   Q751M2|Q751M2_ASHGO               Score: 9      Queries matched: 1
 AGL325Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 19   980.96   1959.90   1959.95   -0.05 1  9  2.1 6  D.FWDPFRKLHQQLTED.F + Deamidation (NQ)

11.   Q75AM4|Q75AM4_ASHGO               Mass: 30979    Score: 9      Queries matched: 1
 ADL101Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   623.32   1244.63   1244.61   0.01 1  9  2.7 4  E.NLESNLQALIE.L + 2 Deamidation (NQ)

12.   Q75CM2|Q75CM2_ASHGO               Mass: 79488    Score: 9      Queries matched: 2
 ACL103Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
15   567.94   1700.79   1700.90   -0.11 1  9  3.1 1  E.VQLVSFKLFLYDTE.S
 16   851.41   1700.81   1700.90   -0.09 1  (2) 14 4  E.VQLVSFKLFLYDTE.S

13.   Q75EK8|Q75EK8_ASHGO               Score: 8      Queries matched: 1
 AAR071Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   619.77   1237.53   1237.62   -0.09 1  10  2.4 2  E.KLFSGSKVNEE.D + Deamidation (NQ)

14.   Q757G9|Q757G9_ASHGO               Score: 8      Queries matched: 1
 AER044Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 18   871.38   1740.74   1740.89   -0.15 1  8  3.8 2  D.RHIKQAEKSVGMTQE.E

15.   Q751N8|Q751N8_ASHGO               Score: 7      Queries matched: 1
 AGL348Wp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 13   830.32   1658.63   1658.83   -0.20 0  9  4.1 2  D.FTRQLYKMVLQSE.G + Deamidation (NQ); Oxidation (M)

16.   Q753A9|Q753A9_ASHGO               Mass: 48152    Score: 7      Queries matched: 1
 AFR415Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   612.34   1222.66   1222.66   0.01 0  7  3.6 1  E.GASYALAKKVSE.L

17.   Q74Z46|Q74Z46_ASHGO               Score: 6      Queries matched: 1
 AGR360Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   612.34   1222.66   1222.55   0.11 0  6  4.9 2  E.QGFTVLSNMPE.R + Deamidation (NQ)

18.   Q754X5|Q754X5_ASHGO               Mass: 144124   Score: 5      Queries matched: 2
 AFL059Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 15   567.94   1700.79   1700.80   -0.01 1  5  6.7 2  E.KYLQIALDNNHNQE.L + 2 Deamidation (NQ)
16   851.41   1700.81   1700.80   0.01 1  (4) 8.7 1  E.KYLQIALDNNHNQE.L + 2 Deamidation (NQ)

19.   Q759H7|Q759H7_ASHGO               Score: 5      Queries matched: 1
 ADR300Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   612.34   1222.66   1222.52   0.14 1  5  5.5 3  D.QTSMDRNVGSE.R

20.   Q752J4|Q752J4_ASHGO               Score: 5      Queries matched: 1
 AFR580Cp - Ashbya gossypii (Yeast) (Eremothecium gossypii)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 19   980.96   1959.90   1959.90   -0.00 1  5  5.8 8  E.EPKHMMVGKTNTTDSGLD.M

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   674.32   1346.62   1346.57   0.05 0  4  9.7 1  NSVTQKLYMMD + 2 Deamidation (NQ); Oxidation (M)
12   706.37   1410.73   1410.69   0.03 0  3  11 1  FFIHYITANRE + Deamidation (NQ)
4   558.28   1114.55   1114.57   -0.02 0  3  12 1  SPGAKARAATAD
10   683.83   1365.65   1365.68   -0.03 1  2  14 1  LSQQFRDKLTE + 2 Deamidation (NQ)
1   959.79   958.78        
14   838.90   1675.79        
22   1437.63   2873.24        
23   1257.96   3770.87        
24   978.61   3910.42        
25   1440.39   4318.16        
26   1080.55   4318.19        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : V8-DE
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Deamidation (NQ),Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.8 Da
Fragment Mass Tolerance: ± 0.4 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 26

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 2
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 14.2   -0.04    1  Ssr1 D.VAHFCTKE.S
Top scoring peptide matches to query 3
Score greater than 17 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 17.8   -0.03    3  Fig2 E.GLKVTAPDCE.A
  4.0   -0.05            E.AGLSLGALQSGD.A
Top scoring peptide matches to query 4
Score greater than 13 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.6   -0.02        D.SPGAKARAATAD.V
  0.3    0.07        E.SVNQKNFFE.S
Top scoring peptide matches to query 5
Score greater than 15 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.9   -0.04    3  Fig2 -.ATYNVKLDYE.Q
Top scoring peptide matches to query 6
Score greater than 18 indicates homology
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein             Peptide
  7.0    0.01   16  Q753A9|Q753A9_ASHGO E.GASYALAKKVSE.L
  5.6    0.11   17  Q74Z46|Q74Z46_ASHGO E.QGFTVLSNMPE.R
  5.2    0.14   19  Q759H7|Q759H7_ASHGO D.QTSMDRNVGSE.R
  1.5    0.12                           E.SDSSKWLSGQE.L
Top scoring peptide matches to query 7
Score greater than 25 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein             Peptide
 53.4   -0.04    1  Ssr1                E.GALSAFKANCAE.H
  9.6   -0.09   13  Q75EK8|Q75EK8_ASHGO E.KLFSGSKVNEE.D
  5.0   -0.10                           E.TRAAFVKNTTE.E
  3.7   -0.05                           E.TVQAATYLQNE.Q
  3.7   -0.05                           E.TVQAATYLQNE.Q
  2.0   -0.13                           E.SLQAEHKVVLD.R
  1.6   -0.14                           D.FKLSSTWLKE.Y
  1.3   -0.16                           D.LQLLPGEVIQE.F
  1.2   -0.03                           E.CVSIGMVKEAE.L
  0.5   -0.02                           D.NTIMGSLQTEE.K
Top scoring peptide matches to query 8
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein             Peptide
 19.0   -0.05    5  Q751A2|Q751A2_ASHGO D.IRSMAQLAILE.L
 15.9   -0.02    6  Q75AU0|Q75AU0_ASHGO E.SVEQNKLALIE.K
  9.8   -0.04    8  Ecm33               D.SLKLMDLPSLE.A
  9.0    0.01   11  Q75AM4|Q75AM4_ASHGO E.NLESNLQALIE.L
  8.2    0.01   11  Q75AM4|Q75AM4_ASHGO E.NLESNLQALIE.L
  3.4    0.01   11  Q75AM4|Q75AM4_ASHGO E.NLESNLQALIE.L
Top scoring peptide matches to query 9
Score greater than 14 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.7    0.05        E.NSVTQKLYMMD.D
  2.4    0.05        E.NSVTQKLYMMD.D
  1.2   -0.08        E.DSLSSNVKLQKE.L
Top scoring peptide matches to query 10
Score greater than 14 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.0   -0.03        E.LSQQFRDKLTE.F
Top scoring peptide matches to query 11
Score greater than 19 indicates homology
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 42.5   -0.04    4  Gas1 E.FSRLPVPAFFSE.Y
  3.7    0.05            E.QAPVDSAVHGQASE.H
  3.6    0.11            M.SFNAHYGAQSAQD.Y
  1.7    0.18            -.MSNVNCYFQSE.I
  1.7    0.18            -.MSNVNCYFQSE.I
Top scoring peptide matches to query 12
Score greater than 14 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.6    0.03        D.FFIHYITANRE.R
  1.2    0.08        D.KSERNGFFPAQE.E
Top scoring peptide matches to query 13
Score greater than 26 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein             Peptide
 70.9   -0.04    1  Ssr1                E.SDAMQKCFSSICPE.G
  8.6   -0.20   15  Q751N8|Q751N8_ASHGO D.FTRQLYKMVLQSE.G
  3.7   -0.19                           E.TNSTLNLSLLIEPED.S
  1.8   -0.19                           D.LMHQLVTLISPYDE.K
  1.6   -0.19                           D.ERHTPVIEPIQNPE.A
  1.6   -0.19                           D.ERHTPVIEPIQNPE.A
  0.5    0.79                           E.TNSTLNLSLLIEPED.S
  0.2   -0.27                           E.FSTILAEFHKLKPE.Y
Top scoring peptide matches to query 14
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 15
Score greater than 18 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein             Peptide
  8.6   -0.11   12  Q75CM2|Q75CM2_ASHGO E.VQLVSFKLFLYDTE.S
  5.2   -0.01   18  Q754X5|Q754X5_ASHGO E.KYLQIALDNNHNQE.L
  0.0    0.05                           D.TAAPQSTANPDPSVQTD.R
Top scoring peptide matches to query 16
Score greater than 16 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein             Peptide
  4.1    0.01   18  Q754X5|Q754X5_ASHGO E.KYLQIALDNNHNQE.L
  4.1    0.01   18  Q754X5|Q754X5_ASHGO E.KYLQIALDNNHNQE.L
  3.0    0.08                           D.TAAPQSTANPDPSVQTD.R
  2.0   -0.09   12  Q75CM2|Q75CM2_ASHGO E.VQLVSFKLFLYDTE.S
  0.7    0.01   18  Q754X5|Q754X5_ASHGO E.KYLQIALDNNHNQE.L
  0.6   -0.09                           D.QLVSVIELQQITSLE.S
Top scoring peptide matches to query 17
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 71.3   -0.06    1  Ssr1 E.SSHPLNDVAHFCTKE.S
Top scoring peptide matches to query 18
Score greater than 24 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein             Peptide
 65.1   -0.04    1  Ssr1                E.SSHPLNDVAHFCTKE.S
  8.0   -0.15   14  Q757G9|Q757G9_ASHGO D.RHIKQAEKSVGMTQE.E
  2.7   -0.13                           E.HEKLNIWIAMLNLE.N
  2.1   -0.13                           D.SATNFSKALNRFQKE.D
Top scoring peptide matches to query 19
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein             Peptide
 76.3   -0.04    3  Fig2                E.QYVDVFYHFKSGGVSVE.A
 12.8    0.11    7  Q75CE2|Q75CE2_ASHGO E.GAYYNYHLNMINNNEE.I
 12.8    0.11    7  Q75CE2|Q75CE2_ASHGO E.GAYYNYHLNMINNNEE.I
 12.8    0.11    7  Q75CE2|Q75CE2_ASHGO E.GAYYNYHLNMINNNEE.I
  9.5   -0.01    9  Q74ZH1|Q74ZH1_ASHGO E.NSWLSDAGLVANILNQNE.G
  9.3   -0.05   10  Q751M2|Q751M2_ASHGO D.FWDPFRKLHQQLTED.F
  8.9   -0.01    9  Q74ZH1|Q74ZH1_ASHGO E.NSWLSDAGLVANILNQNE.G
  5.0   -0.00   20  Q752J4|Q752J4_ASHGO E.EPKHMMVGKTNTTDSGLD.M
  4.2    0.11    7  Q75CE2|Q75CE2_ASHGO E.GAYYNYHLNMINNNEE.I
  4.2    0.11    7  Q75CE2|Q75CE2_ASHGO E.GAYYNYHLNMINNNEE.I
Top scoring peptide matches to query 20
Score greater than 13 indicates homology
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  0.8   -0.06    2  Pir2 E.NIAPQCSPIKLQAIDLVTC.-
Top scoring peptide matches to query 21
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
107.3   -0.03    2  Pir2 E.NIAPQCSPIKLQAIDLVTC.-
Top scoring peptide matches to query 22
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 23
Score greater than 23 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 24
Score greater than 24 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 25
Score greater than 22 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 26
Score greater than 22 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide