Mascot Search Results

User            : 
Email           : 
Search title    : 
MS data file    : cw0195_q1.pkl
Database        : aspergillus  (34936 sequences; 16232982 residues)
Timestamp       : 16 Jan 2007 at 09:31:04 GMT
Significant hits: AN7950  | Aspergillus nidulans (AN7950.3) hypothetical protein (translation) (466 aa)
                  AN1551  | Aspergillus nidulans (AN1551.3) hypothetical protein (translation) (556 aa)
                  AN7323  | Aspergillus nidulans (AN7323.3) hypothetical protein (translation) (210 aa)

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 33 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

  Help
  Significance threshold p< Max. number of hits  
  Standard scoring  MudPIT scoring  Ions score cut-off Show sub-sets
  Show pop-ups  Suppress pop-ups  Sort unassigned Require bold red

        Error tolerant   

1.    AN7950                            Mass: 46771    Score: 252    Queries matched: 4
 | Aspergillus nidulans (AN7950.3) hypothetical protein (translation) (466 aa)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   518.76   1035.50   1035.52   -0.03 0  58  0.00022 1  K.ALFDESVQK.T
12   648.80   1295.58   1295.61   -0.03 0  58  0.00018 1  K.NLETTSGFNSAR.L
13   663.80   1325.59   1325.63   -0.03 0  64  4.8e-005 1  K.AAINQYGDEFAK.L
15   741.36   1480.70   1480.73   -0.03 0  72  6.7e-006 1  K.GFNYGANKPDGTLK.V

 
      Proteins matching the same set of peptides:
      mAN7950                           Mass: 44811    Score: 252    Queries matched: 4
  ScBgl2p homologue

2.    AN1551                            Mass: 58320    Score: 97     Queries matched: 1
 | Aspergillus nidulans (AN1551.3) hypothetical protein (translation) (556 aa)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
14   671.86   1341.70   1341.73   -0.03 0  97  2.2e-008 1  K.AIPGQSQQTTAIK.S

 
      Proteins matching the same set of peptides:
      mAN1551                           Mass: 56676    Score: 97     Queries matched: 1
 Scw11p/Bgl2p homologue
3.    AN7323                            Mass: 21726    Score: 41     Queries matched: 1
 | Aspergillus nidulans (AN7323.3) hypothetical protein (translation) (210 aa)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   640.24   1278.46   1278.48   -0.02 0  41  0.013 1  R.SDGYCDSSNFK.S

 
      Proteins matching the same set of peptides:
      mAN7323                           Mass: 19907    Score: 41     Queries matched: 1
  no BLast homology
4.    An01g03460                        Mass: 24318    Score: 31     Queries matched: 1
 cytoplasmic ribosomal protein of the large subunit L15 rpl15 - Aspergillus niger
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   541.74   1081.46   1081.49   -0.03 0  31  0.087 1  R.QNTQSYWR.Y

 
      Proteins matching the same set of peptides:
      AN0445                            Mass: 24190    Score: 31     Queries matched: 1
 | Aspergillus nidulans (AN0445.3) 60S ribosomal protein L15 (translation) (204 aa)
5.    An07g02150                        Mass: 119195   Score: 21     Queries matched: 1
 similarity to hypothetical AAA family ATPase Cj0377 - Campylobacter jejuni
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   518.76   1035.50   1035.41   0.09 0  21  1 2  R.GSGDGNTDGQK.G + Deamidation (NQ)

6.    66845974|gb|EAL86307.1|           Mass: 92461    Score: 20     Queries matched: 1
 beta-glucosidase, putative [Aspergillus fumigatus Af293]
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 15   741.36   1480.70   1480.70   -0.00 0  20  1.2 2  R.GFDMLHYAIVDGK.Q + Oxidation (M)

7.    An02g10710                        Mass: 66794    Score: 19     Queries matched: 1
 strong similarity to severin kinase - Dictyostelium discoideum
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 14   671.86   1341.70   1341.79   -0.09 1  19  1.4 2  K.AIEKATGEIVAIK.H

8.    66844789|gb|EAL85126.1|           Mass: 16045    Score: 18     Queries matched: 1
 hypothetical protein Afu8g00400 [Aspergillus fumigatus Af293]
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
10   638.83   1275.65   1275.64   0.01 0  18  2.1 1  -.MADIAEQLIEK.L + Oxidation (M)

9.    AN7222                            Mass: 227545   Score: 17     Queries matched: 1
 | Aspergillus nidulans (AN7222.3) conserved hypothetical protein (translation) (2015 aa)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   582.33   1162.64   1162.59   0.05 1  17  2.7 1  R.WQQASRMIK.R + Oxidation (M)

10.   AN2482                            Score: 16     Queries matched: 1
 | Aspergillus nidulans (AN2482.3) conserved hypothetical protein (translation) (2016 aa)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   582.33   1162.64   1162.64   0.00 1  16  3.1 2  K.DTVLTARMLK.Q + Oxidation (M)

11.   AN0124                            Score: 15     Queries matched: 1
 | Aspergillus nidulans (AN0124.3) conserved hypothetical protein (translation) (406 aa)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   582.33   1162.64   1162.46   0.18 0  15  4.3 3  R.EGEDDDENLK.L

12.   AN6395                            Score: 15     Queries matched: 1
 | Aspergillus nidulans (AN6395.3) conserved hypothetical protein (translation) (665 aa)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   582.33   1162.64   1162.58   0.06 0  15  4.3 3  K.WEFPASWLK.E

13.   AN7282                            Score: 15     Queries matched: 1
 | Aspergillus nidulans (AN7282.3) conserved hypothetical protein (translation) (530 aa)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   582.33   1162.64   1162.62   0.03 1  15  4.3 3  K.WETRLMSLK.F

14.   An18g03610                        Score: 14     Queries matched: 1
 similarity to mucin-like protein Muc1 - Saccharomyces cerevisiae
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 14   671.86   1341.70   1341.75   -0.05 1  15  3.7 3  K.ALPETASKLSTPK.K

15.   An11g10610                        Score: 13     Queries matched: 1
 similarity to glycogenin glucosyltransferase - Oryctolagus cuniculus
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 15   741.36   1480.70   1480.76   -0.06 1  13  5.3 3  M.AHNTGVPRITDATK.V + Deamidation (NQ)

16.   AN7335                            Score: 12     Queries matched: 1
 | Aspergillus nidulans (AN7335.3) conserved hypothetical protein (translation) (678 aa)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 14   671.86   1341.70   1341.77   -0.07 1  12  7.6 4  R.AIDWLKNEILK.A

17.   An07g09240                        Mass: 25526    Score: 12     Queries matched: 1
 strong similarity to hypothetical protein SPCC16A11.07 - Schizosaccharomyces pombe
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   582.33   1162.64   1162.51   0.13 0  12  8.9 6  R.WELESQGEGK.G + Deamidation (NQ)

18.   An06g00050                        Score: 12     Queries matched: 1
 hypothetical protein
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   638.83   1275.65   1275.69   -0.05 1  12  8.8 2  K.LQTTVSASRWK.L

19.   AN4279                            Mass: 71517    Score: 12     Queries matched: 1
 | Aspergillus nidulans (AN4279.3) conserved hypothetical protein (translation) (631 aa)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   638.83   1275.65   1275.59   0.06 1  12  8.8 2  R.DGCDAKPTKER.S

20.   AN5491                            Score: 12     Queries matched: 1
 | Aspergillus nidulans (AN5491.3) conserved hypothetical protein (translation) (2103 aa)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   638.83   1275.65   1275.54   0.10 0  12  8.8 2  K.FATYMNEAGEK.E + Oxidation (M)

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   523.25   1044.49   1044.48   0.01 0  9  19 1  SDQPNGGLTR + Deamidation (NQ)
9   587.30   1172.59   1172.61   -0.03 1  7  25 1  RGGSSGPLDLSK
5   526.77   1051.53   1051.58   -0.05 0  6  25 1  ATNHVELLR
1   556.27   555.26        
2   507.26   1012.51        
7   569.82   1137.63        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Deamidation (NQ),Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.8 Da
Fragment Mass Tolerance: ± 0.4 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 15

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 2
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 3
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 57.8   -0.03    1  AN7950     K.ALFDESVQK.T
 21.1    0.09    5  An07g02150 R.GSGDGNTDGQK.G
  9.4   -0.06                  R.ALNFSKLDK.Y
  8.9   -0.05                  R.ALHPERADK.T
  7.0    0.10                  K.NESDNEQAK.L
  7.0    0.10                  K.NESDNEQAK.L
  6.5    0.01                  K.SPFQSVDEK.G
  6.1   -0.07                  R.ALKTYAQNK.G
  5.8   -0.10                  R.LASYLDKVK.S
  5.7   -0.00                  K.DXFNRVQK.I
Top scoring peptide matches to query 4
Score greater than 18 indicates homology
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  8.5    0.01        K.SDQPNGGLTR.G
  4.8   -0.02        K.GHGFQGVTSR.W
  3.5   -0.03        K.AADGLVEGWK.G
  3.5   -0.12        AVSIRVAAMK
  2.0    0.01        K.SDQPNGGLTR.G
  2.0   -0.05        R.AGIDAAITDAK.V
Top scoring peptide matches to query 5
Score greater than 17 indicates homology
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  6.4   -0.05        R.ATNHVELLR.K
  6.1    0.02        R.SKEAFWQR.D
  4.0   -0.10        R.LHRLSTGLR.A
  3.4    0.06        R.NGNAGYGDKR.Q
  3.0    0.08        K.GNTAEESSTR.N
  2.5   -0.03        K.MYSRGLGLR.M
  2.2   -0.02        -.MGKLNYLGR.K
  2.2   -0.00        R.MVSGWFGLR.V
  1.9   -0.00        K.GDNKASYVAK.D
  1.7    0.02        R.TAADREFSR.F
Top scoring peptide matches to query 6
Score greater than 24 indicates homology
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 31.1   -0.03    4  An01g03460 R.QNTQSYWR.Y
  9.5   -0.01                  K.EITTGGMNMK.I
  8.6   -0.15                  R.LTEQHITLK.Y
  8.1   -0.14                  M.NIPQGLPTLK.W
  5.4   -0.03                  K.LQNNVYSDK.E
  5.4   -0.03                  K.LQNNVYSDK.E
  4.7   -0.06                  K.GASPMVSYVR.A
  4.3    0.03                  R.DQFGGSQADR.Q
  4.1   -0.05                  K.LELGENHDR.E
  3.8   -0.17                  K.ELGIDVPVLK.V
Top scoring peptide matches to query 7
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 8
Score greater than 28 indicates homology
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 16.9    0.05    9  AN7222     R.WQQASRMIK.R
 16.3    0.00   10  AN2482     K.DTVLTARMLK.Q
 14.9    0.18   11  AN0124     R.EGEDDDENLK.L
 14.9    0.06   12  AN6395     K.WEFPASWLK.E
 14.9    0.03   13  AN7282     K.WETRLMSLK.F
 11.7    0.13   17  An07g09240 R.WELESQGEGK.G
 10.5   -0.00                  R.GYSNRAAALIK.L
  5.8    0.13                  R.NAQNKTDDVR.D
  5.8    0.08                  K.WEEVSSGTIR.T
  5.8    0.12                  K.WQNIETNTR.G
Top scoring peptide matches to query 9
Score greater than 20 indicates homology
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  7.2   -0.03        K.RGGSSGPLDLSK.L
Top scoring peptide matches to query 10
Score greater than 25 indicates homology
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein                 Peptide
 17.9    0.01    8  66844789|gb|EAL85126.1| -.MADIAEQLIEK.L
 11.7    0.06   19  AN4279                  R.DGCDAKPTKER.S
 11.7    0.10   20  AN5491                  K.FATYMNEAGEK.E
 11.7   -0.05   18  An06g00050              K.LQTTVSASRWK.L
Top scoring peptide matches to query 11
Score greater than 21 indicates homology
Score greater than 35 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 40.7   -0.02    3  AN7323 R.SDGYCDSSNFK.S
  5.5   -0.21              R.NIVLKECFEK.D
  2.9   -0.09              R.TTSTIHMDSDR.T
  2.4    0.74              R.RQHLVVAAINR.A
  1.9   -0.21              R.TVFGNCLEVLK.V
  1.6   -0.14              R.SNTSKSSLNNPK.L
  0.6   -0.16              R.QSQIAAQFNSGK.R
Top scoring peptide matches to query 12
Score greater than 27 indicates homology
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 58.2   -0.03    1  AN7950 K.NLETTSGFNSAR.L
 11.0   -0.12              R.LNEVHMVLLGR.D
  9.3   -0.19              R.NILAEKPRVTR.F
  7.9   -0.00              R.TNFSMAAVNPAR.V
  7.9   -0.14              K.VPTTPNQTIGIR.H
  7.5   -0.14              R.TSAPTRPIAGPTK.S
  7.2   -0.07              R.AREEEAVFFAK.S
  6.2   -0.14              K.GGIQPGTLTRPAK.V
  6.1   -0.10              R.TQSGGKSYIEVK.G
  5.7   -0.25              K.KVNATVILLVAR.E
Top scoring peptide matches to query 13
Score greater than 22 indicates homology
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 63.8   -0.03    1  AN7950 K.AAINQYGDEFAK.L
  5.9   -0.14              R.QSGPTGITSPLLR.Q
  4.7   -0.19              K.AGKLQIFIHTAK.G
  1.4   -0.09              M.SAPQIPNLNTLR.R
Top scoring peptide matches to query 14
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 97.4   -0.03    2  AN1551     K.AIPGQSQQTTAIK.S
 19.2   -0.09    7  An02g10710 K.AIEKATGEIVAIK.H
 15.1   -0.05   14  An18g03610 K.ALPETASKLSTPK.K
 11.9   -0.07   16  AN7335     R.AIDWLKNEILK.A
 11.3   -0.01                  R.AIPFQLPENRR.-
 11.3   -0.00                  K.ALAGAAVEEAQRR.L
  9.9    0.05                  K.AIAIGNPGNGAEEK.E
  9.2    0.01                  R.AIDNTEPSQVIR.K
  9.1    0.05                  K.ALYGTEKSSNSGK.E
  8.7   -0.02                  K.IANMGKPDARIR.L
Top scoring peptide matches to query 15
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein                 Peptide
 72.2   -0.03    1  AN7950                  K.GFNYGANKPDGTLK.V
 19.7   -0.00    6  66845974|gb|EAL86307.1| R.GFDMLHYAIVDGK.Q
 13.2   -0.06   15  An11g10610              M.AHNTGVPRITDATK.V
 10.7    0.00                               K.AVRDACQVVYDGK.G
 10.7   -0.07                               R.MALYGGAVFGPVATK.W
  8.8    0.00                               K.MGNVVDTNGVVFGR.L
  8.7    0.02                               K.SSTRTPVFGNDGNK.S
  5.7   -0.08                               R.QNCNIPAQPAKIK.A
  5.3    0.05                               R.FNNACNPVSGLSAK.E
  4.9    0.00                               K.GYVSVMDGGPTDRK.V