Mascot Search Results

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MS data file    : cw0195_q1.pkl
Database        : anidulans  (9710 sequences; 5130102 residues)
Timestamp       : 8 Apr 2005 at 15:00:13 GMT
Significant hits: AN7950.2	hypothetical  protein	228024	229421	-
                  AN1551.2	hypothetical  protein	62392	64115	-
                  AN7323.2	hypothetical  protein	113713	114452	+

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 30 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

Switch to Protein Summary Report

To create a bookmark for this report, right click this link: Peptide Summary Report (../data/20050408/F002855.dat)

        Error tolerant   

1.    AN7950.2 hypothetical             Mass: 46771    Score: 224    Peptides matched: 4
 protein 228024 229421 -
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   518.76   1035.50   1035.52   -0.03 0  57  0.00011 1  ALFDESVQK
12   648.80   1295.58   1295.61   -0.03 0  48  0.00069 1  NLETTSGFNSAR
13   663.80   1325.59   1325.63   -0.03 0  48  0.00075 1  AAINQYGDEFAK
15   741.36   1480.70   1480.73   -0.03 0  70  4.1e-006 1  GFNYGANKPDGTLK

 
      Proteins matching the same set of peptides:
      mAN7950.2 ScBgl2p                 Mass: 44811    Score: 224    Peptides matched: 4
 homologue

2.    AN1551.2 hypothetical             Mass: 58320    Score: 99     Peptides matched: 1
 protein 62392 64115 -
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
14   671.85   1341.69   1341.73   -0.03 0  99  5.8e-009 1  AIPGQSQQTTAIK

 
      Proteins matching the same set of peptides:
      mAN1551.2                         Mass: 56676    Score: 99     Peptides matched: 1
 Scw11p/Bgl2p homologue
3.    AN7323.2 hypothetical             Mass: 21726    Score: 40     Peptides matched: 1
 protein 113713 114452 +
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   640.24   1278.46   1278.48   -0.02 0  40  0.004 1  SDGYCDSSNFK

 
      Proteins matching the same set of peptides:
      mAN7323.2 no                      Mass: 19907    Score: 40     Peptides matched: 1
 BLast homology
4.    AN0445.2 RL15_ASPNG               Mass: 24190    Score: 26     Peptides matched: 1
 60S RIBOSOMAL PROTEIN L15 202260 203211 -
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   541.74   1081.46   1081.49   -0.03 0  26  0.13 1  QNTQSYWR

5.    AN8102.2 hypothetical             Mass: 49350    Score: 22     Peptides matched: 1
 protein 264377 265753 +
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   556.26   1110.51   1110.55   -0.03 0  22  0.31 1  SYWATTVQR

 
      Proteins matching the same set of peptides:
      mAN8102.2 homology                Mass: 47871    Score: 22     Peptides matched: 1
 with pepsin-like asp. proteases
6.    AN0716.2 hypothetical             Mass: 60622    Score: 17     Peptides matched: 1
 protein 33889 35565 +
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   582.33   1162.64   1163.61   -0.97 1  17  1.2 1  FGIPRGEFNK

7.    AN0124.2 hypothetical             Mass: 45637    Score: 15     Peptides matched: 1
 protein 93846 95205 -
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   582.33   1162.64   1162.46   0.18 0  15  1.8 2  EGEDDDENLK

8.    AN6395.2 hypothetical             Mass: 74220    Score: 15     Peptides matched: 1
 protein 35900 38031 -
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   582.33   1162.64   1162.58   0.06 0  15  1.8 2  WEFPASWLK

9.    AN7282.2 hypothetical             Score: 15     Peptides matched: 1
 protein 38508 40369 -
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   582.33   1162.64   1162.62   0.02 1  15  1.8 2  WETRLMSLK

10.   AN7335.2 hypothetical             Score: 12     Peptides matched: 1
 protein 8055 10109 -
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 14   671.85   1341.69   1341.77   -0.07 1  12  3.3 2  AIDWLKNEILK

11.   AN2461.2 hypothetical             Score: 9      Peptides matched: 1
 protein 113743 119211 +
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 14   671.85   1341.69   1341.69   0.01 0  9  6.1 3  AIDNTEPSQVIR

12.   AN2469.2 hypothetical             Score: 9      Peptides matched: 1
 protein 2144 3019 -
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   541.74   1081.46   1082.57   -1.11 0  9  6.2 2  NEGIALPVDR

13.   AN4891.2 hypothetical             Score: 8      Peptides matched: 1
 protein 120776 121529 -
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 12   648.80   1295.58   1295.77   -0.19 1  8  6.8 2  NILAEKPRVTR

14.   AN5254.2 hypothetical             Score: 8      Peptides matched: 1
 protein 23094 26311 -
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 15   741.36   1480.70   1480.78   -0.08 1  8  6.5 2  QNCNIPAQPAKIK

15.   AN0282.2 predicted                Score: 8      Peptides matched: 1
 protein 416884 418871 -
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 12   648.80   1295.58   1295.72   -0.14 0  8  8.2 3  TSAPTRPIAGPTK

16.   AN4279.2 hypothetical             Mass: 73509    Score: 6      Peptides matched: 1
 protein 12937 15351 +
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
10   638.83   1275.65   1275.59   0.06 1  6  12 1  DGCDAKPTKER

17.   AN6037.2 G6PI_ASPOR               Score: 6      Peptides matched: 1
 Glucose-6-phosphate isomerase (GPI) (Phosphoglucose isomerase) (PGI) (Phosphohexose isomerase) (PHI) 15514 17227 +
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   640.24   1278.46   1278.66   -0.21 1  6  11 2  NIVLKECFEK

18.   AN3320.2 hypothetical             Score: 6      Peptides matched: 1
 protein 141707 149400 +
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   518.76   1035.50   1035.61   -0.11 0  9  7.3 2  ALNHLLDLK

19.   AN6317.2 hypothetical             Score: 6      Peptides matched: 1
 protein similar to AF429307_1 chitin synthase 238292 240485 -
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   541.74   1081.46   1081.49   -0.03 0  6  12 4  NSHFSTFSR

20.   AN5893.2 FLBA_EMENI               Score: 6      Peptides matched: 1
 Developmental regulator flbA 329870 332215 +
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 15   741.36   1480.70   1481.75   -1.05 1  6  11 3  FVDARFIESVDGK

Peptide matches not assigned to protein hits: (no details means no match)
List sorted by Decreasing Score. Sort by Decreasing Intensity or Increasing Query # / Mr

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   587.30   1172.59   1172.61   -0.03 1  7  11 1  RGGSSGPLDLSK
4   526.77   1051.52   1051.58   -0.06 0  6  12 1  ATNHVELLR
3   523.25   1044.49   1044.51   -0.01 0  2  41 1  QTPTASDPTK
1   507.26   1012.51         
7   569.82   1137.63         

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Data File Name         : cw0195_q1.pkl
Number of queries      : 15

Mascot:  http://www.matrixscience.com/ 

Top scoring peptide matches to query 1
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
Top scoring peptide matches to query 2
Score greater than 25 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 57.4   -0.03   1             AN7950.2	hypothetical  ALFDESVQK
  9.1   -0.11  18             AN3320.2	hypothetical  ALNHLLDLK
  6.0   -0.07                                        ALKTYAQNK
  5.9   -0.10                                        LASYLDKVK
  3.3    0.01                                        RTEEEGTSK
  2.2   -0.10                                        ALIAAERHR
  2.1   -0.99                                        VYEAENQGK
  1.9    0.07                                        AMEQEASDR
  1.4    0.92                                        AEIAYSLLR
  1.3   -0.00                                        DSGFGGIDLR
Top scoring peptide matches to query 3
Score greater than 14 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  2.3   -0.01                                        QTPTASDPTK
  1.8    0.94                                        LAELDEGVAK
  1.3   -0.05                                        VSLQPGESTK
  1.0   -0.02                                        GHGFQGVTSR
Top scoring peptide matches to query 4
Score greater than 18 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  6.3   -0.06                                        ATNHVELLR
  4.7    0.96                                        SYAKAWIGR
  4.6    1.05                                        DDDLSPPHR
  3.8    1.04                                        SFGATGGANNR
  3.6    0.98                                        YASLNSAIGR
  2.1   -1.05                                        HLNGISSGLR
  1.5   -0.01                                        ATVTEGFTAR
  1.4    0.07                                        DGMFQNANR
  1.3    0.99                                        GGGVAFGPHPR
  1.1    0.93                                        GNKVLHIDR
Top scoring peptide matches to query 5
Score greater than 23 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 25.8   -0.03   4               AN0445.2	RL15_ASPNG  QNTQSYWR
  9.0   -1.11  12             AN2469.2	hypothetical  NEGIALPVDR
  7.0   -1.10                                        EAHWKVASR
  6.0   -0.03  19             AN6317.2	hypothetical  NSHFSTFSR
  5.6   -0.17                                        ELGIDVPVLK
  5.4   -1.12                                        LSLATGGHATR
  4.7   -0.17                                        KAATPRPSVR
  4.0   -0.07                                        QMRHHAFR
  4.0   -1.06                                        DQGLVMAGHR
  2.6   -1.12                                        FITAFGKGSR
Top scoring peptide matches to query 6
Score greater than 20 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 22.1   -0.03   5             AN8102.2	hypothetical  SYWATTVQR
  6.0   -0.01                                        THHDTTPFR
  2.7   -0.13                                        HLEKYIVLP
  0.4   -0.02                                        SYTEQTQVR
  0.1   -0.05                                        HADLAENLTK
Top scoring peptide matches to query 7
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
Top scoring peptide matches to query 8
Score greater than 28 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 16.7   -0.97   6             AN0716.2	hypothetical  FGIPRGEFNK
 14.8    0.18   7             AN0124.2	hypothetical  EGEDDDENLK
 14.8    0.06   8             AN6395.2	hypothetical  WEFPASWLK
 14.8    0.02   9             AN7282.2	hypothetical  WETRLMSLK
  4.6   -0.01                                        GYSNRAAALIK
  4.4    0.07                                        IMEWASGLEK
  4.2    0.00                                        STAFASGGKIPK
  4.2    0.00                                        AFQALPEFLK
  4.2    0.00                                        NLSQVQSFLK
  3.4    0.00                                        KLEYEQVVR
Top scoring peptide matches to query 9
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  7.2   -0.03                                        RGGSSGPLDLSK
  3.9    0.97                                        IATASSVPSSPR
  3.9   -0.99                                        DSRSSEQPLR
Top scoring peptide matches to query 10
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  6.4    0.06  16             AN4279.2	hypothetical  DGCDAKPTKER
Top scoring peptide matches to query 11
Score greater than 21 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 40.3   -0.02   3             AN7323.2	hypothetical  SDGYCDSSNFK
  6.1   -0.21  17               AN6037.2	G6PI_ASPOR  NIVLKECFEK
Top scoring peptide matches to query 12
Score greater than 24 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 48.3   -0.03   1             AN7950.2	hypothetical  NLETTSGFNSAR
  8.4   -0.19  13             AN4891.2	hypothetical  NILAEKPRVTR
  7.6   -0.14  15                AN0282.2	predicted  TSAPTRPIAGPTK
  1.4   -1.14                                        LDEWPQLKIR
  1.1   -0.25                                        KVNATVILLVAR
  0.4   -0.04                                        KVEQYEQNMK
Top scoring peptide matches to query 13
Score greater than 16 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 48.0   -0.03   1             AN7950.2	hypothetical  AAINQYGDEFAK
Top scoring peptide matches to query 14
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 99.3   -0.03   2             AN1551.2	hypothetical  AIPGQSQQTTAIK
 11.8   -0.07  10             AN7335.2	hypothetical  AIDWLKNEILK
  9.1    0.01  11             AN2461.2	hypothetical  AIDNTEPSQVIR
  2.8    0.04                                        AIGAAFSSFDAASK
  2.8    0.08                                        KHSNDVEAGEEK
  2.5    0.03                                        ALHEMESPTVTK
  2.4   -1.09                                        GQIAELKALSVSK
  2.3   -0.03                                        IADLEQQLREK
  2.3    0.00                                        ALELVHMMKDR
  2.0    0.04                                        ALCAGFFMQVAK
Top scoring peptide matches to query 15
Score greater than 25 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 70.4   -0.03   1             AN7950.2	hypothetical  GFNYGANKPDGTLK
  8.4   -0.08  14             AN5254.2	hypothetical  QNCNIPAQPAKIK
  6.0   -1.05  20               AN5893.2	FLBA_EMENI  FVDARFIESVDGK
  4.4   -0.02                                        EGYDKLAALMAGDK
  1.9    0.94                                        AFFDKHPSGPHLK
  0.9    0.83                                        AVGVVTVPTKPLDGK
  0.2   -0.10                                        LRLVNEAVDTHSK
  0.2   -0.03                                        EMLVMEFPSASLK