Mascot Search Results

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MS data file    : cw0306_q2.pkl
Database        : CAlbicans dos251005 (20288 sequences; 6720871 residues)
Timestamp       : 14 Nov 2006 at 16:40:16 GMT
Significant hits: mSSR1     |Candida albicans 212 aa - Secretory Stress Response
                  mRBT5     |predicted| C. albicans fungus|CA2558| 241 aa
                  mEcm33.3  |predicted|m5366.2|CaECM33
                  mPga24    |predicted| mIPF5185 C. albicans |CA1678| 533 aa

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 30 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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1.    mSSR1                             Mass: 20690    Score: 149    Queries matched: 2
 |Candida albicans 212 aa - Secretory Stress Response
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   635.87   1269.72   1269.66   0.07 0  32  0.031 1  -.APPACLLACVAK.V
16   814.90   1627.79   1627.70   0.09 0  116  1.2e-010 1  K.CSGLNDLSCICTTK.N

2.    mRBT5                             Mass: 22745    Score: 67     Queries matched: 3
 |predicted| C. albicans fungus|CA2558| 241 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   618.83   1235.65   1235.59   0.07 0  10  5 1  R.IYDQLPECAK.E
18   584.98   1751.92   1751.82   0.09 1  37  0.011 1  R.IYDQLPECAKECVK.Q
26   1184.02   3549.03   3548.53   0.50 0  21  0.54 1  K.QSTSSTPCPYWDTGCLCVMPQFAGAVGNCVAK.N

 
      Proteins matching the same set of peptides:
      19952.1                           Mass: 24614    Score: 67     Queries matched: 3
 |Rbt5| CONTIG=Contig10231 POSCDS1=6478 POSCDS2=7203
      21544.1                           Mass: 25457    Score: 67     Queries matched: 3
 CONTIG=Contig20231 POSCDS1=6478 POSCDS2=7203
3.    mEcm33.3                          Mass: 41984    Score: 58     Queries matched: 1
 |predicted|m5366.2|CaECM33
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
12   787.45   1572.89   1572.81   0.08 0  58  8e-005 1  K.TIGGALQISDNSELR.S

 
      Proteins matching the same set of peptides:
      5366.2                            Mass: 43798    Score: 58     Queries matched: 1
 |Ecm33.3| CaECM33 CONTIG=Contig10163 POSCDS1=164904 POSCDS2=166142
      24311.1                           Mass: 42880    Score: 58     Queries matched: 1
 CONTIG=Contig20163 POSCDS1=164513 POSCDS2=165751
4.    mPga24                            Mass: 52805    Score: 35     Queries matched: 1
 |predicted| mIPF5185 C. albicans |CA1678| 533 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   635.87   1269.73   1269.64   0.09 0  35  0.017 1  K.VITVVACDEHK.C

 
      Proteins matching the same set of peptides:
      5185.2                            Mass: 54872    Score: 35     Queries matched: 1
 |Pga24/Ycw1| CONTIG=Contig10184 POSCDS1=48416 POSCDS2=50017
      23664.1                           Mass: 56212    Score: 35     Queries matched: 1
 CONTIG=Contig20184 POSCDS1=48545 POSCDS2=50146
5.    8527.1                            Mass: 21461    Score: 30     Queries matched: 1
 |Pga29| CONTIG=Contig10225 POSCDS1=30293 POSCDS2=30907
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
10   638.82   1275.62   1275.56   0.06 0  30  0.063 1  R.YSESEYAVSNK.K

 
      Proteins matching the same set of peptides:
      mIPF8527|mPga29:                  Mass: 19928    Score: 30     Queries matched: 1
 189 aa - Candida albicans unknown function
      21875.1                           Mass: 22824    Score: 30     Queries matched: 1
 CONTIG=Contig20225 POSCDS1=30286 POSCDS2=30900
6.    mPhr2                             Mass: 57243    Score: 21     Queries matched: 1
 |validated by us| C albicans |CA3867| 544 aa pH-regulated protein 2
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   782.93   1563.85   1563.75   0.09 0  21  0.46 1  R.SIPVGYSANDDSAIR.V

 
      Proteins matching the same set of peptides:
      4604.2                            Mass: 59477    Score: 21     Queries matched: 1
 |Phr2| CONTIG=Contig20237 POSCDS1=156145 POSCDS2=157779
      14612.3                           Mass: 67274    Score: 21     Queries matched: 1
 CONTIG=Contig10237 POSCDS1=160121 POSCDS2=161755
7.    12297.1                           Mass: 15525    Score: 21     Queries matched: 1
 CONTIG=Contig10166 POSCDS1=62870 POSCDS2=63313
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 18   584.98   1751.92   1751.82   0.09 1  21  0.47 2  R.IYDQIPKCAQECVK.Q + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      24214.1                           Mass: 16067    Score: 21     Queries matched: 1
 CONTIG=Contig20166 POSCDS1=63254 POSCDS2=63697
8.    4129.1                            Mass: 48889    Score: 14     Queries matched: 1
 CONTIG=Contig10139 POSCDS1=89222 POSCDS2=90478
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 9   635.87   1269.73   1269.77   -0.04 1  14  2.2 2  K.LISVEKLNNLK.V

 
      Proteins matching the same set of peptides:
      25271.1                           Score: 14     Queries matched: 1
9.    14452.2                           Mass: 6744     Score: 8      Queries matched: 1
 CONTIG=Contig10230 POSCDS1=154864 POSCDS2=155055
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 9   635.87   1269.73   1269.61   0.12 0  8  7.8 3  K.QGEPVALKPTDN.- + 2 Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      17243.2                           Mass: 6744     Score: 8      Queries matched: 1
 CONTIG=Contig20230 POSCDS1=155162 POSCDS2=155353
10.   6901.3                            Mass: 33703    Score: 8      Queries matched: 1
 CONTIG=Contig10192 POSCDS1=53156 POSCDS2=53932
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   631.85   1261.68   1261.70   -0.03 1  8  9.6 1  K.VLYLNLDQRK.K + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      14249.2                           Mass: 29127    Score: 8      Queries matched: 1
 CONTIG=Contig20192 POSCDS1=53157 POSCDS2=53933
11.   3636.2                            Score: 8      Queries matched: 1
 CONTIG=Contig10254 POSCDS1=19872 POSCDS2=22001
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   631.85   1261.68   1261.58   0.09 0  8  11 2  K.DWDELELSQK.S

 
      Proteins matching the same set of peptides:
      20323.1                           Score: 8      Queries matched: 1
12.   19584.1                           Score: 6      Queries matched: 1
 CONTIG=Contig10143 POSCDS1=61144 POSCDS2=63636
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 18   584.98   1751.92   1751.82   0.09 0  6  13 4  R.TTQSTLNFEPLGDSNK.R + Deamidation (NQ)

13.   8067.1                            Score: 6      Queries matched: 1
 CONTIG=Contig10187 POSCDS1=13746 POSCDS2=16727
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   631.85   1261.68   1261.76   -0.08 1  6  16 3  R.IRYLILMVNK.L

 
      Proteins matching the same set of peptides:
      23569.1                           Score: 6      Queries matched: 1
14.   TRBOTR                            Mass: 24662    Score: 5      Queries matched: 1
 trypsin (EC3.4.21.4 ) precursor-bovine
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
21   1082.60   2163.19   2163.03   0.15 0  5  19 1  R.LGEDNINVVEGNEQFISASK.S + Deamidation (NQ)

15.   2330.2                            Mass: 9814     Score: 5      Queries matched: 1
 CONTIG=Contig20093 POSCDS1=31539 POSCDS2=31748
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
15   806.39   1610.76   1610.64   0.12 1  5  17 1  K.KCPEDGQQCLSCK.T + 2 Deamidation (NQ)

16.   14673.2                           Score: 5      Queries matched: 1
 CONTIG=Contig1867 POSCDS1=2250 POSCDS2=3386
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   618.83   1235.65   1235.64   0.01 0  5  19 2  K.FQELLNTITR.E + 2 Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      14858.2                           Mass: 47158    Score: 5      Queries matched: 1
 CONTIG=Contig2010 POSCDS1=9667 POSCDS2=10854
17.   9465.1                            Score: 5      Queries matched: 1
 CONTIG=Contig10196 POSCDS1=5686 POSCDS2=6048
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 21   1082.60   2163.19   2163.04   0.15 0  5  21 3  R.YFQVQELLLDDYYPVTR.F + 2 Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      19219.1                           Score: 5      Queries matched: 1
18.   19585.2                           Score: 4      Queries matched: 1
 CONTIG=Contig20143 POSCDS1=60861 POSCDS2=63353
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 18   584.98   1751.92   1751.86   0.06 0  4  21 5  R.TTQSTLNFKPLGDSNK.R + 2 Deamidation (NQ)

19.   2593.2                            Score: 4      Queries matched: 1
 CONTIG=Contig10253 POSCDS1=34575 POSCDS2=36650
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   631.85   1261.68   1261.69   -0.01 0  4  23 4  K.LSTQSSAAVLSAK.T

 
      Proteins matching the same set of peptides:
      20474.1                           Score: 4      Queries matched: 1
20.   19971.1                           Mass: 18219    Score: 4      Queries matched: 1
 CONTIG=Contig10248 POSCDS1=174069 POSCDS2=174557
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
13   797.93   1593.84   1593.95   -0.11 1  4  22 1  R.KFFNISVVIVTLSK.M

 
      Proteins matching the same set of peptides:
      20658.1                           Mass: 18219    Score: 4      Queries matched: 1
 CONTIG=Contig20248 POSCDS1=166623 POSCDS2=167111
Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
17   855.46   1708.90   1708.83   0.07 0  4  23 1  DLYAAGGIDMSENILK
23   1220.16   2438.31   2438.17   0.14 0  3  26 1  FEQAEELVGIFNVNLTEEDIK + 2 Deamidation (NQ)
1   531.30   1060.58   1060.55   0.03 0  1  46 1  QISITQQSR + Deamidation (NQ)
2   540.80   1079.60   1079.44   0.16 0  0  57 1  STSNNNGEEK + Deamidation (NQ)
3   547.81   1093.60   1093.49   0.12 0  0  58 1  YGLCGPNGAGK + Deamidation (NQ)
4   596.82   1191.63        
7   633.33   1264.65        
14   532.29   1593.84        
19   930.44   1858.86        
20   974.53   1947.05        
22   758.44   2272.29        
24   1000.52   2998.53        
25   1023.53   3067.57        
27   1203.03   3606.06        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Deamidation (NQ),Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.8 Da
Fragment Mass Tolerance: ± 0.4 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 27

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 14 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.5    0.03        K.QISITQQSR.N
Top scoring peptide matches to query 2
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  0.3    0.16        STSNNNGEEK
  0.3    0.16        STSNNNGEEK
  0.0    0.06        K.LSVIEFVNC.-
Top scoring peptide matches to query 3
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  0.2    0.12        R.YGLCGPNGAGK.S
Top scoring peptide matches to query 4
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 5
Score greater than 18 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 10.4    0.07    2  mRBT5   IYDQLPECAK
  4.8    0.01   16  14673.2 K.FQELLNTITR.E
  0.8    0.13               K.ENFPNDIEQK.T
  0.0    0.02               K.FENETELLLK.Y
Top scoring peptide matches to query 6
Score greater than 19 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  7.9   -0.03   10  6901.3 K.VLYLNLDQRK.K
  7.5    0.09   11  3636.2 K.DWDELELSQK.S
  5.7   -0.08   13  8067.1 R.IRYLILMVNK.L
  4.1   -0.01   19  2593.2 K.LSTQSSAAVLSAK.T
  2.3   -0.09              -.LLATFIKTINK.K
  1.6    0.11              K.QRFGDQQPQR.Q
  0.5    0.03              K.NVAHHVVSQAAK.Q
Top scoring peptide matches to query 7
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 8
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 32.4    0.07    1  mSSR1 -.APPACLLACVAK.V
  2.5    0.06             M.APVVQQPAPSFK.K
  2.2    0.11             K.APKFLTYCNR.F
Top scoring peptide matches to query 9
Score greater than 29 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 35.0    0.09    4  mPga24  K.VITVVACDEHK.C
 14.0   -0.04    8  4129.1  K.LISVEKLNNLK.V
  8.4    0.12    9  14452.2 K.QGEPVALKPTDN.-
  2.1   -0.00               R.IVLSQVNEIQK.H
  1.9    0.05               R.SLPPGSMTGAPKK.R
  0.4    0.01               K.VITPSISDLTPK.I
  0.2    0.09               R.SNISLPMVEHK.G
Top scoring peptide matches to query 10
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 29.9    0.06    5  8527.1 R.YSESEYAVSNK.K
Top scoring peptide matches to query 11
Score greater than 15 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 20.9    0.09    6  mPhr2 R.SIPVGYSANDDSAIR.V
  1.1    0.08             K.SLNTMTLVNERNR.K
  0.9    0.08             K.SLNTMTLVNERNR.K
  0.8    0.01             K.TVSIGSLGSMTISRR.F
  0.6    0.20             K.AQMTNNANAGNAANAK.G
  0.5    0.09             R.ISLSEMTVPYGDPR.A
  0.3    0.14             R.SLEENTKESNNGDK.A
  0.1    0.10             K.SINEESEKYSHLK.D
Top scoring peptide matches to query 12
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 58.4    0.08    3  mEcm33.3 K.TIGGALQISDNSELR.S
Top scoring peptide matches to query 13
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  4.1   -0.11   20  19971.1 R.KFFNISVVIVTLSK.M
Top scoring peptide matches to query 14
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 15
Score greater than 16 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  4.8    0.12   15  2330.2 K.KCPEDGQQCLSCK.T
  3.4    0.08              R.NGIMDPNSFTEEEK.K
  1.7   -0.08              R.WSLKDLAFEFLSR.R
  1.1   -0.14              R.QVVAKSIQNASQPIK.G
  1.1   -0.14              R.QVVAKSIQNASQPIK.G
  1.1   -0.14              R.QVVAKSIQNASQPIK.G
Top scoring peptide matches to query 16
Score greater than 22 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
116.3    0.09    1  mSSR1 K.CSGLNDLSCICTTK.N
  2.7    0.08             R.TEQGNDNDSLQIHR.D
  0.7    0.79             K.LLVLLLNARSISSTK.E
Top scoring peptide matches to query 17
Score greater than 14 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.7    0.07        K.DLYAAGGIDMSENILK.V
  1.2    0.01        K.SNTSKFLETQLEAIK.L
  1.1    0.04        K.WGFTPLNRDEYIAK.K
  0.6    0.17        R.ESSKSPSETNGNVDEK.V
Top scoring peptide matches to query 18
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 37.1    0.09    2  mRBT5   IYDQLPECAKECVK
 20.7    0.09    7  12297.1 R.IYDQIPKCAQECVK.Q
 12.3    0.09    7  12297.1 R.IYDQIPKCAQECVK.Q
  6.4    0.09   12  19584.1 R.TTQSTLNFEPLGDSNK.R
  4.2    0.06   18  19585.2 R.TTQSTLNFKPLGDSNK.R
  3.2    0.00               K.ISMDAVQQLLFKTNK.F
  3.2    0.00               K.ISMDAVQQLLFKTNK.F
  2.5   -0.01               K.IYNLYLFLSGQFFK.N
  2.4    0.05               K.IYSRWILQSYNFY.-
  2.4    0.13               K.IQKDFSNFSAWHNR.T
Top scoring peptide matches to query 19
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 20
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 21
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  5.2    0.15   14  TRBOTR R.LGEDNINVVEGNEQFISASK.S
  4.8    0.15   14  TRBOTR R.LGEDNINVVEGNEQFISASK.S
  4.7    0.15   17  9465.1 R.YFQVQELLLDDYYPVTR.F
  4.5    0.15   14  TRBOTR R.LGEDNINVVEGNEQFISASK.S
  3.5    0.15   14  TRBOTR R.LGEDNINVVEGNEQFISASK.S
Top scoring peptide matches to query 22
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 23
Score greater than 15 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.3    0.14        K.FEQAEELVGIFNVNLTEEDIK.Y
  2.6    0.14        K.FEQAEELVGIFNVNLTEEDIK.Y
  2.4   -0.73        R.LSGGSNSSGSSSANTAIGGGGGGGGSENR.N
  2.0    0.11        K.ILGNTWNYKTLASQILNEMNL.-
  1.7    0.11        K.ILGNTWNYKTLASQILNEMNL.-
  0.9    0.14        K.FEQAEELVGIFNVNLTEEDIK.Y
Top scoring peptide matches to query 24
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 25
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 26
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 20.5    0.50    2  mRBT5 K.QSTSSTPCPYWDTGCLCVMPQFAGAVGNCVAK.N
  5.0   -0.48    2  mRBT5 K.QSTSSTPCPYWDTGCLCVMPQFAGAVGNCVAK.N
  4.6   -0.48    2  mRBT5 K.QSTSSTPCPYWDTGCLCVMPQFAGAVGNCVAK.N
  4.0   -0.48    2  mRBT5 K.QSTSSTPCPYWDTGCLCVMPQFAGAVGNCVAK.N
Top scoring peptide matches to query 27
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide