Mascot Search Results

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MS data file    : cw0305_q2.pkl
Database        : CAlbicans dos251005 (20288 sequences; 6720871 residues)
Timestamp       : 14 Nov 2006 at 15:04:48 GMT
Significant hits: mRBT5  |predicted| C. albicans fungus|CA2558| 241 aa
                  mPhr2  |validated by us| C albicans |CA3867| 544 aa pH-regulated protein 2

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 30 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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1.    mRBT5                             Mass: 22745    Score: 75     Queries matched: 2
 |predicted| C. albicans fungus|CA2558| 241 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   618.83   1235.65   1235.59   0.07 0  43  0.0029 1  R.IYDQLPECAK.E
16   584.97   1751.90   1751.82   0.08 1  33  0.03 1  R.IYDQLPECAKECVK.Q

 
      Proteins matching the same set of peptides:
      mPga10                            Mass: 23610    Score: 75     Queries matched: 2
 |predicted| C. albicans fungus|CA4381|mIPF20169: 250 aa
      19952.1                           Mass: 24614    Score: 75     Queries matched: 2
 |Rbt5| CONTIG=Contig10231 POSCDS1=6478 POSCDS2=7203
      20169.1                           Mass: 25603    Score: 75     Queries matched: 2
 |Pga10| CONTIG=Contig10231 POSCDS1=59560 POSCDS2=60312
      21543.1                           Mass: 25731    Score: 75     Queries matched: 2
 CONTIG=Contig20231 POSCDS1=59564 POSCDS2=60316
      21544.1                           Mass: 25457    Score: 75     Queries matched: 2
 CONTIG=Contig20231 POSCDS1=6478 POSCDS2=7203

2.    mPhr2                             Mass: 57243    Score: 33     Queries matched: 1
 |validated by us| C albicans |CA3867| 544 aa pH-regulated protein 2
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
14   809.47   1616.93   1616.84   0.09 0  35  0.02 1  R.DIPYLEAVDTNVIR.V

 
      Proteins matching the same set of peptides:
      4604.2                            Mass: 59477    Score: 33     Queries matched: 1
 |Phr2| CONTIG=Contig20237 POSCDS1=156145 POSCDS2=157779
      14612.3                           Mass: 67274    Score: 33     Queries matched: 1
 CONTIG=Contig10237 POSCDS1=160121 POSCDS2=161755
3.    12297.1                           Mass: 15525    Score: 22     Queries matched: 1
 CONTIG=Contig10166 POSCDS1=62870 POSCDS2=63313
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 16   584.97   1751.90   1751.82   0.08 1  22  0.31 2  R.IYDQIPKCAQECVK.Q + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      24214.1                           Mass: 16067    Score: 22     Queries matched: 1
 CONTIG=Contig20166 POSCDS1=63254 POSCDS2=63697
4.    8527.1                            Mass: 21461    Score: 22     Queries matched: 1
 |Pga29| CONTIG=Contig10225 POSCDS1=30293 POSCDS2=30907
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   679.93   1357.84   1357.75   0.09 1  22  0.45 1  K.KTDDSAPITIVAK.F

 
      Proteins matching the same set of peptides:
      mIPF8527|mPga29:                  Mass: 19928    Score: 22     Queries matched: 1
 189 aa - Candida albicans unknown function
      21875.1                           Mass: 22824    Score: 22     Queries matched: 1
 CONTIG=Contig20225 POSCDS1=30286 POSCDS2=30900
5.    4222.2                            Mass: 152716   Score: 16     Queries matched: 1
 CONTIG=Contig10259 POSCDS1=8804 POSCDS2=12805
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   547.31   1092.61   1092.56   0.05 1  16  1.6 1  R.FAQKLSQDR.N + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      20209.1                           Mass: 153746   Score: 16     Queries matched: 1
 CONTIG=Contig20259 POSCDS1=8803 POSCDS2=12804
6.    11991.3                           Mass: 133429   Score: 15     Queries matched: 1
 CONTIG=Contig20184 POSCDS1=65365 POSCDS2=68817
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   587.86   1173.72   1173.58   0.14 0  15  1.9 1  K.VNEVLNDEIK.I + 2 Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      28720.1                           Mass: 133315   Score: 15     Queries matched: 1
 CONTIG=Contig10184 POSCDS1=65195 POSCDS2=68647
7.    11025.2                           Score: 15     Queries matched: 1
 CONTIG=Contig10233 POSCDS1=37721 POSCDS2=39712
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   587.86   1173.72   1173.56   0.16 0  15  2 2  K.VDASYYNLTK.E + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      21449.1                           Score: 15     Queries matched: 1
8.    12310.2                           Score: 11     Queries matched: 1
 CONTIG=Contig10217 POSCDS1=9312 POSCDS2=11090
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   587.86   1173.72   1173.59   0.13 0  13  3.4 3  K.VNQIINQQSK.L + 3 Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      22186.1                           Score: 11     Queries matched: 1
9.    6901.3                            Mass: 33703    Score: 11     Queries matched: 1
 CONTIG=Contig10192 POSCDS1=53156 POSCDS2=53932
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   631.84   1261.67   1261.70   -0.03 1  11  5.3 1  K.VLYLNLDQRK.K + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      14249.2                           Mass: 29127    Score: 11     Queries matched: 1
 CONTIG=Contig20192 POSCDS1=53157 POSCDS2=53933
10.   TRBOTR                            Mass: 24662    Score: 9      Queries matched: 1
 trypsin (EC3.4.21.4 ) precursor-bovine
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
20   758.43   2272.28   2272.15   0.13 0  9  8.2 1  K.SIVHPSYNSNTLNNDIMLIK.L

11.   3636.2                            Score: 9      Queries matched: 1
 CONTIG=Contig10254 POSCDS1=19872 POSCDS2=22001
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   631.84   1261.67   1261.58   0.09 0  9  7.6 2  K.DWDELELSQK.S

 
      Proteins matching the same set of peptides:
      20323.1                           Score: 9      Queries matched: 1
12.   5499.1                            Mass: 13395    Score: 8      Queries matched: 1
 CONTIG=Contig10141 POSCDS1=78809 POSCDS2=79147
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   587.86   1173.72   1173.59   0.13 0  8  9.3 7  K.VNQISEQDLK.L + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      18593.2                           Mass: 13496    Score: 8      Queries matched: 1
 CONTIG=Contig20141 POSCDS1=78810 POSCDS2=79148
13.   10175.2                           Score: 8      Queries matched: 1
 CONTIG=Contig20063 POSCDS1=29537 POSCDS2=30943
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   587.86   1173.72   1173.61   0.10 0  8  9.4 8  K.TINDDEILIK.I + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      17304.3                           Score: 8      Queries matched: 1
14.   11261.2                           Score: 8      Queries matched: 1
 CONTIG=Contig10221 POSCDS1=7464 POSCDS2=9779
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 14   809.47   1616.93   1616.83   0.10 1  9  7.4 2  K.LNELSVQTNDKINK.N + 2 Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      21999.1                           Score: 8      Queries matched: 1
15.   8970.1                            Score: 8      Queries matched: 1
 CONTIG=Contig10053 POSCDS1=61286 POSCDS2=64057
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   547.31   1092.61   1092.53   0.08 0  8  11 3  R.MAGFPENALK.A + Oxidation (M)

 
      Proteins matching the same set of peptides:
      26756.1                           Score: 8      Queries matched: 1
16.   15676.2                           Score: 7      Queries matched: 1
 CONTIG=Contig10123 POSCDS1=223452 POSCDS2=224639
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   587.86   1173.72   1173.66   0.05 0  8  9.4 8  K.LTGDQLSSILK.N

 
      Proteins matching the same set of peptides:
      25635.1                           Score: 7      Queries matched: 1
17.   402.1                             Mass: 134371   Score: 7      Queries matched: 1
 CONTIG=Contig2516 POSCDS1=100054 POSCDS2=103692
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
21   1220.15   2438.29   2438.20   0.09 1  7  12 1  K.ILGNTWNYKTLASQILNEMNL.- + 3 Deamidation (NQ)

18.   27570.1                           Score: 6      Queries matched: 1
 CONTIG=Contig1370 POSCDS1=1 POSCDS2=357
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   547.31   1092.61   1092.57   0.03 0  8  10 2  R.FAGCGSLLLR.T

19.   11826.1                           Score: 5      Queries matched: 1
 CONTIG=Contig10248 POSCDS1=179964 POSCDS2=180848
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   547.31   1092.61   1092.51   0.10 0  5  18 4  -.MSQEDLLTR.V + Deamidation (NQ)

20.   15457.2                           Score: 4      Queries matched: 1
 CONTIG=Contig10161 POSCDS1=82084 POSCDS2=83856
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   547.31   1092.61   1092.51   0.10 1  4  22 6  K.SMKIDQENK.F + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      17922.3                           Score: 4      Queries matched: 1
Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   531.30   1060.58   1060.44   0.14 0  4  28 1  GNENSNPATR + 2 Deamidation (NQ)
12   797.92   1593.83   1593.95   -0.12 1  3  28 1  KFFNISVVIVTLSK
8   633.33   1264.64   1264.67   -0.03 1  3  32 1  IMTTSVDKLNK + Oxidation (M)
19   1027.04   2052.06   2052.00   0.06 1  2  30 1  TTNLKANNIYPSNSISNAK + 3 Deamidation (NQ)
17   930.43   1858.85   1858.90   -0.05 1  2  37 1  KSSTSLTNMTNILSMSK + Deamidation (NQ); Oxidation (M)
5   596.82   1191.63   1191.41   0.22 0  2  40 1  MMQQFGGMGGM + 2 Deamidation (NQ); Oxidation (M)
13   532.29   1593.84   1593.90   -0.05 0  1  42 1  PSLDDILKPVAEAVK
15   855.94   1709.86   1709.85   0.01 1  0  49 1  EIEEKLANFETMIK + Oxidation (M)
3   559.30   1116.59        
10   716.90   1431.79        
11   761.41   1520.80        
18   974.52   1947.03        
22   1023.73   4090.90        
23   1423.19   4266.54        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Deamidation (NQ),Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.8 Da
Fragment Mass Tolerance: ± 0.4 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 23

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 13 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.6    0.14        K.GNENSNPATR.V
  0.1    0.06        K.IQYPPNNSK.R
  0.1    0.08        K.LQENLDNSK.T
Top scoring peptide matches to query 2
Score greater than 21 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 15.7    0.05    5  4222.2  R.FAQKLSQDR.N
  7.9    0.03   18  27570.1 R.FAGCGSLLLR.T
  7.6    0.08   15  8970.1  R.MAGFPENALK.A
  5.3    0.10   19  11826.1 -.MSQEDLLTR.V
  5.3    0.03               R.KFADIGNTVK.K
  4.5    0.10   20  15457.2 K.SMKIDQENK.F
  4.5    0.10   20  15457.2 K.SMKIDQENK.F
  2.3    0.09               R.NSNHRHATR.V
  1.8    0.11               K.QSSELSNAQK.N
  1.6    0.13               K.NSEDIMQQK.S
Top scoring peptide matches to query 3
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 4
Score greater than 26 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 15.3    0.14    6  11991.3 K.VNEVLNDEIK.I
 15.2    0.16    7  11025.2 K.VDASYYNLTK.E
 12.8    0.13    8  12310.2 K.VNQIINQQSK.L
 12.8    0.13    8  12310.2 K.VNQIINQQSK.L
 11.5    0.13    8  12310.2 K.VNQIINQQSK.L
  9.7    0.13    8  12310.2 K.VNQIINQQSK.L
  8.5    0.13   12  5499.1  K.VNQISEQDLK.L
  8.4    0.05   16  15676.2 K.LTGDQLSSILK.N
  8.4    0.10   13  10175.2 K.TINDDEILIK.I
  8.1    0.13    8  12310.2 K.VNQIINQQSK.L
Top scoring peptide matches to query 5
Score greater than 14 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.8    0.22        K.MMQQFGGMGGM.-
Top scoring peptide matches to query 6
Score greater than 19 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 42.8    0.07    1  mRBT5 IYDQLPECAK
  3.7    0.03             K.FPFLQNDLNK.F
  2.1    0.01             K.IDYQSIQIEK.S
  0.1    0.02             K.FENETELLLK.Y
Top scoring peptide matches to query 7
Score greater than 22 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 10.5   -0.03    9  6901.3 K.VLYLNLDQRK.K
  8.9    0.09   11  3636.2 K.DWDELELSQK.S
  5.1    0.02              K.IIDDSNLSTKR.K
  3.5    0.06              K.LSSVASGNGDNLK.D
  2.9   -0.09              R.IRYLILMVNK.L
  2.9   -0.09              -.LLATFIKTINK.K
  1.9   -0.01              R.KSSVHHQTIPK.F
  0.7    0.03              K.NVAHHVVSQAAK.Q
  0.3    0.14              R.MNGIENEQNGR.L
  0.3    0.14              R.MNGIENEQNGR.L
Top scoring peptide matches to query 8
Score greater than 15 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.7   -0.03        R.IMTTSVDKLNK.L
  2.3   -0.79        R.NNNNNNNNSNK.Y
  1.4   -0.02        K.STIFSVVSSPNK.S
Top scoring peptide matches to query 9
Score greater than 15 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 21.9    0.09    4  8527.1 K.KTDDSAPITIVAK.F
  0.8   -0.78              R.QQQQQQQRQR.D
Top scoring peptide matches to query 10
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 11
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 12
Score greater than 16 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.1   -0.12        R.KFFNISVVIVTLSK.M
Top scoring peptide matches to query 13
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.4   -0.05        M.PSLDDILKPVAEAVK.N
  0.6    0.12        K.ALDLRNTQQQQMK.K
  0.6    0.02        R.ALINADKSSYEAALK.D
Top scoring peptide matches to query 14
Score greater than 24 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 34.9    0.09    2  mPhr2   R.DIPYLEAVDTNVIR.V
  9.3    0.10   14  11261.2 K.LNELSVQTNDKINK.N
  8.3    0.10   14  11261.2 K.LNELSVQTNDKINK.N
  2.4    0.03               K.LVYLINNIVSDKPK.S
  2.1    0.11               -.LDLMVYHLQNNLK.S
  1.2    0.16               K.QVLETSMQSLHTNK.A
  0.8    0.02               K.DLLPYSKTSKPQIK.E
Top scoring peptide matches to query 15
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  0.5    0.01        K.EIEEKLANFETMIK.K
Top scoring peptide matches to query 16
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 32.6    0.08    1  mRBT5   IYDQLPECAKECVK
 22.5    0.08    3  12297.1 R.IYDQIPKCAQECVK.Q
  7.8    0.08    3  12297.1 R.IYDQIPKCAQECVK.Q
  3.0    0.15               K.LNYQQFENICFMK.F
  3.0    0.15               K.LNYQQFENICFMK.F
  2.8    0.08               R.TTQSTLNFEPLGDSNK.R
  2.6    0.08               R.TTQSTLNFEPLGDSNK.R
  1.4    0.03               K.LSFGDGDSIASASQRLK.M
  1.4    0.03               R.IYKLQNMDNLVNLR.V
  1.4    0.03               R.IYKLQNMDNLVNLR.V
Top scoring peptide matches to query 17
Score greater than 14 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.9   -0.05        R.KSSTSLTNMTNILSMSK.L
Top scoring peptide matches to query 18
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 19
Score greater than 15 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.2    0.06        K.TTNLKANNIYPSNSISNAK.Y
Top scoring peptide matches to query 20
Score greater than 22 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  9.2    0.13   10  TRBOTR K.SIVHPSYNSNTLNNDIMLIK.L
Top scoring peptide matches to query 21
Score greater than 19 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  6.5    0.09   17  402.1 K.ILGNTWNYKTLASQILNEMNL.-
Top scoring peptide matches to query 22
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 23
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide