Mascot Search Results

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MS data file    : cw0304_q2.pkl
Database        : CAlbicans dos251005 (20288 sequences; 6720871 residues)
Timestamp       : 14 Nov 2006 at 13:39:26 GMT
Significant hits: mSSR1       |Candida albicans 212 aa - Secretory Stress Response
                  mPhr2       |validated by us| C albicans |CA3867| 544 aa pH-regulated protein 2
                  mRBT5       |predicted| C. albicans fungus|CA2558| 241 aa
                  mSod4/Pga2  |predicted| mIPF1218 C. albicans |CA4835| 232 aa
                  8527.1      |Pga29| CONTIG=Contig10225 POSCDS1=30293 POSCDS2=30907

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 30 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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1.    mSSR1                             Mass: 20690    Score: 255    Queries matched: 3
 |Candida albicans 212 aa - Secretory Stress Response
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   635.87   1269.73   1269.66   0.07 0  56  0.00014 1  -.APPACLLACVAK.V
16   814.91   1627.80   1627.70   0.10 0  106  1.3e-009 1  K.CSGLNDLSCICTTK.N
17   855.44   1708.86   1708.76   0.09 0  93  2.7e-008 1  K.EICPNGDADTAISAFK.S + Deamidation (NQ)

2.    mPhr2                             Mass: 57243    Score: 137    Queries matched: 3
 |validated by us| C albicans |CA3867| 544 aa pH-regulated protein 2
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   653.32   1304.63   1304.55   0.07 0  51  0.00039 1  K.ASGYESATNDYK.N
10   782.93   1563.84   1563.75   0.09 0  62  3.9e-005 1  R.SIPVGYSANDDSAIR.V
15   809.51   1617.00   1616.84   0.16 0  25  0.23 1  R.DIPYLEAVDTNVIR.V

 
      Proteins matching the same set of peptides:
      4604.2                            Mass: 59477    Score: 137    Queries matched: 3
 |Phr2| CONTIG=Contig20237 POSCDS1=156145 POSCDS2=157779
      14612.3                           Mass: 67274    Score: 137    Queries matched: 3
 CONTIG=Contig10237 POSCDS1=160121 POSCDS2=161755
3.    mRBT5                             Mass: 22745    Score: 63     Queries matched: 3
 |predicted| C. albicans fungus|CA2558| 241 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   618.84   1235.66   1235.59   0.08 0  26  0.15 1  R.IYDQLPECAK.E
18   584.98   1751.92   1751.82   0.10 1  (22) 0.33 1  R.IYDQLPECAKECVK.Q
19   876.97   1751.92   1751.82   0.10 1  37  0.0099 1  R.IYDQLPECAKECVK.Q

 
      Proteins matching the same set of peptides:
      mPga10                            Mass: 23610    Score: 63     Queries matched: 3
 |predicted| C. albicans fungus|CA4381|mIPF20169: 250 aa
      19952.1                           Mass: 24614    Score: 63     Queries matched: 3
 |Rbt5| CONTIG=Contig10231 POSCDS1=6478 POSCDS2=7203
      20169.1                           Mass: 25603    Score: 63     Queries matched: 3
 |Pga10| CONTIG=Contig10231 POSCDS1=59560 POSCDS2=60312
      21543.1                           Mass: 25731    Score: 63     Queries matched: 3
 CONTIG=Contig20231 POSCDS1=59564 POSCDS2=60316
      21544.1                           Mass: 25457    Score: 63     Queries matched: 3
 CONTIG=Contig20231 POSCDS1=6478 POSCDS2=7203
4.    mSod4/Pga2                        Mass: 22239    Score: 59     Queries matched: 1
 |predicted| mIPF1218 C. albicans |CA4835| 232 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   650.39   1298.76   1298.68   0.08 0  59  7.5e-005 1  K.TPAALELGDLSGR.H

 
      Proteins matching the same set of peptides:
      1218.1                            Mass: 23826    Score: 59     Queries matched: 1
 |Pga2/Sod4| CONTIG=Contig10139 POSCDS1=127254 POSCDS2=127952
      25297.1                           Mass: 25136    Score: 59     Queries matched: 1
 CONTIG=Contig20139 POSCDS1=127009 POSCDS2=127707
5.    8527.1                            Mass: 21461    Score: 35     Queries matched: 1
 |Pga29| CONTIG=Contig10225 POSCDS1=30293 POSCDS2=30907
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   638.83   1275.65   1275.56   0.09 0  35  0.018 1  R.YSESEYAVSNK.K

 
      Proteins matching the same set of peptides:
      mIPF8527|mPga29:                  Mass: 19928    Score: 35     Queries matched: 1
 189 aa - Candida albicans unknown function
      21875.1                           Mass: 22824    Score: 35     Queries matched: 1
 CONTIG=Contig20225 POSCDS1=30286 POSCDS2=30900
6.    12297.1                           Mass: 15525    Score: 27     Queries matched: 2
 CONTIG=Contig10166 POSCDS1=62870 POSCDS2=63313
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 18   584.98   1751.92   1751.82   0.10 1  (16) 1.5 2  R.IYDQIPKCAQECVK.Q + Deamidation (NQ)
 19   876.97   1751.92   1751.82   0.10 1  27  0.12 2  R.IYDQIPKCAQECVK.Q + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      24214.1                           Mass: 16067    Score: 27     Queries matched: 2
 CONTIG=Contig20166 POSCDS1=63254 POSCDS2=63697
7.    mEcm33.3                          Mass: 41984    Score: 24     Queries matched: 1
 |predicted|m5366.2|CaECM33
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   787.46   1572.90   1572.81   0.09 0  24  0.21 1  K.TIGGALQISDNSELR.S

 
      Proteins matching the same set of peptides:
      5366.2                            Mass: 43798    Score: 24     Queries matched: 1
 |Ecm33.3| CaECM33 CONTIG=Contig10163 POSCDS1=164904 POSCDS2=166142
      24311.1                           Mass: 42880    Score: 24     Queries matched: 1
 CONTIG=Contig20163 POSCDS1=164513 POSCDS2=165751
8.    19584.1                           Mass: 93366    Score: 22     Queries matched: 2
 CONTIG=Contig10143 POSCDS1=61144 POSCDS2=63636
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 18   584.98   1751.92   1751.82   0.10 0  (7) 10 3  R.TTQSTLNFEPLGDSNK.R + Deamidation (NQ)
 19   876.97   1751.92   1751.82   0.10 0  22  0.39 3  R.TTQSTLNFEPLGDSNK.R + Deamidation (NQ)

9.    19585.2                           Score: 18     Queries matched: 2
 CONTIG=Contig20143 POSCDS1=60861 POSCDS2=63353
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 18   584.98   1751.92   1751.86   0.06 0  (1) 42 6  R.TTQSTLNFKPLGDSNK.R + 2 Deamidation (NQ)
 19   876.97   1751.92   1751.86   0.06 0  18  0.99 4  R.TTQSTLNFKPLGDSNK.R + 2 Deamidation (NQ)

10.   13042.2                           Mass: 102761   Score: 12     Queries matched: 1
 CONTIG=Contig20148 POSCDS1=47790 POSCDS2=50471
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 19   876.97   1751.92   1751.81   0.11 1  12  3.8 5  K.NYKILNQQYQYMK.E + 3 Deamidation (NQ); Oxidation (M)

 
      Proteins matching the same set of peptides:
      29110.1                           Mass: 103986   Score: 12     Queries matched: 1
 CONTIG=Contig10148 POSCDS1=47768 POSCDS2=50455
11.   10888.1                           Mass: 85036    Score: 10     Queries matched: 1
 CONTIG=Contig10218 POSCDS1=4505 POSCDS2=6775
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   716.89   1431.77   1431.72   0.05 0  10  5.5 1  K.QSPVSSTGVDVSIR.D + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      22116.1                           Mass: 88705    Score: 10     Queries matched: 1
 CONTIG=Contig20218 POSCDS1=4505 POSCDS2=6775
12.   19715.2                           Score: 7      Queries matched: 1
 CONTIG=Contig10241 POSCDS1=115431 POSCDS2=121751
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 17   855.44   1708.86   1708.91   -0.06 0  9  7.2 2  R.QLFAQVTNPPIDPIR.E + Deamidation (NQ)

 
      Proteins matching the same set of peptides:
      20929.1                           Score: 7      Queries matched: 1
13.   5909.2                            Score: 5      Queries matched: 1
 CONTIG=Contig10259 POSCDS1=42835 POSCDS2=43773
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   638.83   1275.65   1275.72   -0.07 1  5  18 2  -.MSLVAIKNGISK.L + Oxidation (M)

14.   15363.2                           Mass: 40824    Score: 5      Queries matched: 1
 |Pir1| CONTIG=Contig20045 POSCDS1=52307 POSCDS2=53464
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   540.81   1079.60   1079.53   0.07 0  5  20 1  K.LSVIEFVNC.-

 
      Proteins matching the same set of peptides:
      m15363.2                          Mass: 38940    Score: 5      Queries matched: 1
 |mPir1| 367 aa Candida albicans
      19968.1                           Mass: 36680    Score: 5      Queries matched: 1
 |Pir1| CONTIG=Contig10045 POSCDS1=52996 POSCDS2=54036
      m19968.1                          Mass: 34796    Score: 5      Queries matched: 1
 |mPir1| 328 aa CONTIG=Contig10045 POSCDS1=52996 POSCDS2=54036
15.   24798.1                           Score: 5      Queries matched: 1
 CONTIG=Contig20151 POSCDS1=36092 POSCDS2=36535
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 18   584.98   1751.92   1751.93   -0.01 0  5  19 4  K.IYNLYLFLSGQFFK.N

 
      Proteins matching the same set of peptides:
      29063.1                           Score: 5      Queries matched: 1
16.   7763.2                            Mass: 53445    Score: 5      Queries matched: 1
 CONTIG=Contig10193 POSCDS1=63588 POSCDS2=64991
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
14   806.39   1610.76   1610.82   -0.06 0  5  18 1  K.EATPDVQQVAETVPK.T

 
      Proteins matching the same set of peptides:
      23392.1                           Mass: 54032    Score: 5      Queries matched: 1
 CONTIG=Contig20193 POSCDS1=63599 POSCDS2=65002
17.   2857.1                            Score: 4      Queries matched: 1
 CONTIG=Contig2511 POSCDS1=9440 POSCDS2=10216
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 9   716.89   1431.77   1431.68   0.09 1  4  21 2  K.GEESVEQGENKVK.A

18.   380.1                             Score: 3      Queries matched: 1
 CONTIG=Contig2516 POSCDS1=118955 POSCDS2=121846
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 16   814.91   1627.80   1627.74   0.06 1  3  23 2  K.SAVEGRNENNHLSAK.S + 3 Deamidation (NQ)

19.   19971.1                           Mass: 18219    Score: 3      Queries matched: 1
 CONTIG=Contig10248 POSCDS1=174069 POSCDS2=174557
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
13   797.92   1593.83   1593.95   -0.12 1  3  29 1  R.KFFNISVVIVTLSK.M

 
      Proteins matching the same set of peptides:
      20658.1                           Mass: 18219    Score: 3      Queries matched: 1
 CONTIG=Contig20248 POSCDS1=166623 POSCDS2=167111
20.   21908.1                           Score: 3      Queries matched: 1
 CONTIG=Contig20225 POSCDS1=50093 POSCDS2=50443
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 9   716.89   1431.77   1431.74   0.03 0  3  29 3  -.GNYLFPLTLHQK.C + 2 Deamidation (NQ)

Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
20   758.44   2272.30   2272.15   0.15 0  1  55 1  SIVHPSYNSNTLNNDIMLIK
8   664.39   1326.76   1326.67   0.09 1  1  43 1  KPEMPENGRLR + Deamidation (NQ)
3   633.34   1264.66        
12   532.28   1593.83        
21   1137.16   2272.31        
22   1220.16   2438.31        
23   1000.51   2998.50        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Deamidation (NQ),Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 0.8 Da
Fragment Mass Tolerance: ± 0.4 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 23

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  4.9    0.07   14  15363.2 K.LSVIEFVNC.-
Top scoring peptide matches to query 2
Score greater than 15 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 25.8    0.08    3  mRBT5 IYDQLPECAK
  1.3    0.06             K.INQFITNQQK.L
  0.9   -0.07             R.HRLLTEIINK.D
  0.7    0.11             K.GRNEMIQSER.L
Top scoring peptide matches to query 3
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 4
Score greater than 18 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 55.9    0.07    1  mSSR1 -.APPACLLACVAK.V
  2.6    0.07             M.APVVQQPAPSFK.K
  2.3    0.11             K.APKFLTYCNR.F
  2.2    0.14             K.KGNSNDHQQIK.D
  1.6    0.14             K.KGNSNDHQQIK.D
Top scoring peptide matches to query 5
Score greater than 20 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 35.4    0.09    5  8527.1 R.YSESEYAVSNK.K
  5.3   -0.07   13  5909.2 -.MSLVAIKNGISK.L
  2.0    0.07              -.MVNFSYQKNK.N
  0.8    0.07              -.MVNFSYQKNK.N
Top scoring peptide matches to query 6
Score greater than 19 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 59.0    0.08    4  mSod4/Pga2 K.TPAALELGDLSGR.H
  2.8    0.07                  R.AGVLEPEGMVGIK.Y
Top scoring peptide matches to query 7
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 51.4    0.07    2  mPhr2 K.ASGYESATNDYK.N
Top scoring peptide matches to query 8
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.0    0.09        R.KPEMPENGRLR.R
  1.0    0.07        K.KPESQNAPGSKGK.D
Top scoring peptide matches to query 9
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 10.1    0.05   11  10888.1 K.QSPVSSTGVDVSIR.D
  4.3    0.09   17  2857.1  K.GEESVEQGENKVK.A
  2.9    0.03   20  21908.1 -.GNYLFPLTLHQK.C
  1.3    0.08               K.QVSDAWVAAGKGDK.I
  1.3    0.10               R.KLGNNDGQLNLNK.Q
  1.0    0.01               K.ENIPSFLEKLDK.Q
  0.9   -0.02               K.ILSICFLNPISR.H
  0.5    0.12               -.MYINQYLNIDK.L
  0.5    0.12               -.MYINQYLNIDK.L
Top scoring peptide matches to query 10
Score greater than 18 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 61.7    0.09    2  mPhr2 R.SIPVGYSANDDSAIR.V
  1.5    0.05             K.SLSLSQSQSQQSRK.I
  0.9    0.10             K.SINEESEKYSHLK.D
Top scoring peptide matches to query 11
Score greater than 14 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 24.3    0.09    7  mEcm33.3 K.TIGGALQISDNSELR.S
  0.4    0.13                R.KDFLYSSSSLSPNK.S
  0.2    0.04                K.NVGALQGIQFLRQK.G
  0.2    0.04                K.NVGALQGIQFLRQK.G
Top scoring peptide matches to query 12
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 13
Score greater than 15 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.0   -0.12   19  19971.1 R.KFFNISVVIVTLSK.M
Top scoring peptide matches to query 14
Score greater than 16 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  4.6   -0.06   16  7763.2 K.EATPDVQQVAETVPK.T
  2.8   -0.09              R.WSLKDLAFEFLSR.R
  1.1    0.08              R.NGIMDPNSFTEEEK.K
Top scoring peptide matches to query 15
Score greater than 17 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 25.4    0.16    2  mPhr2 R.DIPYLEAVDTNVIR.V
  2.6   -0.64             K.DNENENVKSTHDSE.-
  1.2    0.11             R.NIFEKGQSIIDVVR.S
  0.5    0.17             K.LNELSVQTNDKINK.N
Top scoring peptide matches to query 16
Score greater than 22 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
106.0    0.10    1  mSSR1 K.CSGLNDLSCICTTK.N
  3.4    0.06   18  380.1 K.SAVEGRNENNHLSAK.S
  1.2    0.02             M.TTPSYTKNLNMNTK.S
  0.5   -0.06             R.DTNVPVELNKVSLAK.S
Top scoring peptide matches to query 17
Score greater than 27 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 92.9    0.09    1  mSSR1   K.EICPNGDADTAISAFK.S
  8.7   -0.06   12  19715.2 R.QLFAQVTNPPIDPIR.E
  3.8   -0.06   12  19715.2 R.QLFAQVTNPPIDPIR.E
  3.7   -0.06   12  19715.2 R.QLFAQVTNPPIDPIR.E
  0.6   -0.01               R.SLLANSSATNLNAYLR.G
  0.2   -0.12               R.YIYITLVALKEIDR.R
Top scoring peptide matches to query 18
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 22.3    0.10    3  mRBT5   IYDQLPECAKECVK
 15.7    0.10    6  12297.1 R.IYDQIPKCAQECVK.Q
  7.3    0.10    8  19584.1 R.TTQSTLNFEPLGDSNK.R
  4.7   -0.01   15  24798.1 K.IYNLYLFLSGQFFK.N
  3.6    0.10    8  19584.1 R.TTQSTLNFEPLGDSNK.R
  1.2    0.06    9  19585.2 R.TTQSTLNFKPLGDSNK.R
  0.1    0.03               R.SWLPSIYLCITGTNK.L
Top scoring peptide matches to query 19
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 37.5    0.10    3  mRBT5   IYDQLPECAKECVK
 26.7    0.10    6  12297.1 R.IYDQIPKCAQECVK.Q
 21.5    0.10    8  19584.1 R.TTQSTLNFEPLGDSNK.R
 17.5    0.06    9  19585.2 R.TTQSTLNFKPLGDSNK.R
 11.7    0.11   10  13042.2 K.NYKILNQQYQYMK.E
 11.7    0.11   10  13042.2 K.NYKILNQQYQYMK.E
 11.7    0.11   10  13042.2 K.NYKILNQQYQYMK.E
 10.4    0.10    8  19584.1 R.TTQSTLNFEPLGDSNK.R
  3.9    0.11   10  13042.2 K.NYKILNQQYQYMK.E
  3.9    0.11   10  13042.2 K.NYKILNQQYQYMK.E
Top scoring peptide matches to query 20
Score greater than 14 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.1    0.15        K.SIVHPSYNSNTLNNDIMLIK.L
Top scoring peptide matches to query 21
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 22
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 23
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide