Mascot Search Results

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MS data file    : cw0260_q1.pkl
Database        : CAlbicans dos251005 (20288 sequences; 6720871 residues)
Timestamp       : 14 Nov 2005 at 16:12:52 GMT
Significant hits: mCHT2    583 aa - chitinase 2 precursor Candida albicans
                  mSSR1    |Candida albicans 212 aa - Secretory Stress Response
                  TRBOTR   trypsin (EC3.4.21.4 ) precursor-bovine
                  11189.3  CONTIG=Contig10212 POSCDS1=259364 POSCDS2=274477

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 30 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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1.    mCHT2                             Mass: 59565    Score: 199    Queries matched: 5
 583 aa - chitinase 2 precursor Candida albicans
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   495.30   988.58   988.54   0.04 0  63  4.3e-005 1  K.LSSAIEEIK.C
3   497.76   993.51   993.49   0.02 0  43  0.0042 1  K.FADTLWNK.F
4   510.30   1018.58   1018.54   0.04 0  19  1.1 1  K.GENFVVQVK.N
19   970.03   1938.05   1938.01   0.04 0  60  4.6e-005 1  K.LFVGVPATSNIAGYVDTSK.L
26   1107.58   2213.15   2213.12   0.03 0  15  1 1  K.TVLLSLGGGVGDYGFSDVASATK.F

 
      Proteins matching the same set of peptides:
      15860.2                           Mass: 61554    Score: 199    Queries matched: 5
 |Cht2| CONTIG=Contig10194 POSCDS1=2376 POSCDS2=4127
      19719.2                           Mass: 51350    Score: 199    Queries matched: 5
 CONTIG=Contig20194 POSCDS1=2710 POSCDS2=4131

2.    mSSR1                             Mass: 20690    Score: 89     Queries matched: 1
 |Candida albicans 212 aa - Secretory Stress Response
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   635.88   1269.75   1269.66   0.09 0  89  7.6e-008 1  -.APPACLLACVAK.V

3.    TRBOTR                            Mass: 24662    Score: 66     Queries matched: 2
 trypsin (EC3.4.21.4 ) precursor-bovine
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   577.33   1152.65   1152.57   0.08 0  44  0.0027 1  K.SSGTSYPDVLK.C
13   745.89   1489.77   1489.73   0.04 0  21  0.51 1  K.LQGIVSWGSGCAQK.N

4.    11189.3                           Mass: 575592   Score: 31     Queries matched: 1
 CONTIG=Contig10212 POSCDS1=259364 POSCDS2=274477
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
21   985.04   1968.07   1968.05   0.01 1  31  0.033 1  K.SVSKSVIEHSVEVLNDVK.G

 
      Proteins matching the same set of peptides:
      22610.1                           Mass: 428895   Score: 31     Queries matched: 1
 CONTIG=Contig20212 POSCDS1=264139 POSCDS2=275394
5.    mRBT5                             Mass: 22745    Score: 21     Queries matched: 1
 |predicted| C. albicans fungus|CA2558| 241 aa
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
10   618.83   1235.64   1235.59   0.05 0  21  0.56 1  R.IYDQLPECAK.E

 
      Proteins matching the same set of peptides:
      mPga10                            Mass: 23610    Score: 21     Queries matched: 1
 |predicted| C. albicans fungus|CA4381|mIPF20169: 250 aa
      19952.1                           Mass: 24614    Score: 21     Queries matched: 1
 |Rbt5| CONTIG=Contig10231 POSCDS1=6478 POSCDS2=7203
      20169.1                           Mass: 25603    Score: 21     Queries matched: 1
 |Pga10| CONTIG=Contig10231 POSCDS1=59560 POSCDS2=60312
      21543.1                           Mass: 25731    Score: 21     Queries matched: 1
 CONTIG=Contig20231 POSCDS1=59564 POSCDS2=60316
      21544.1                           Mass: 25457    Score: 21     Queries matched: 1
 CONTIG=Contig20231 POSCDS1=6478 POSCDS2=7203
6.    20170.1                           Score: 18     Queries matched: 1
 CONTIG=Contig10053 POSCDS1=71020 POSCDS2=71511
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   495.30   988.58   989.48   -0.89 0  18  1.5 3  K.NSIASAGENK.L

 
      Proteins matching the same set of peptides:
      26819.1                           Score: 18     Queries matched: 1
7.    8495.2                            Score: 16     Queries matched: 1
 CONTIG=Contig10163 POSCDS1=196876 POSCDS2=198465
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   495.30   988.58   989.55   -0.97 1  18  1.4 2  R.NSSLSNLKK.K

 
      Proteins matching the same set of peptides:
      24293.1                           Score: 16     Queries matched: 1
8.    19558.1                           Score: 16     Queries matched: 1
 CONTIG=Contig10147 POSCDS1=50671 POSCDS2=52716
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 21   985.04   1968.07   1967.11   0.96 1  16  1 2  K.NLIDKISININQIINDK.Q

 
      Proteins matching the same set of peptides:
      19559.1                           Score: 16     Queries matched: 1
      24944.1                           Score: 16     Queries matched: 1
9.    14540.2                           Score: 12     Queries matched: 1
 CONTIG=Contig10171 POSCDS1=25279 POSCDS2=26745
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   495.30   988.58   988.59   -0.01 1  12  5.4 4  K.ISKLSNISK.Y

 
      Proteins matching the same set of peptides:
      24097.1                           Score: 12     Queries matched: 1
10.   1460.1                            Score: 11     Queries matched: 1
 CONTIG=Contig10237 POSCDS1=76798 POSCDS2=79839
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   495.30   988.58   989.53   -0.95 0  11  7.5 5  K.NNNLQFLK.N

 
      Proteins matching the same set of peptides:
      21148.1                           Score: 11     Queries matched: 1
11.   2807.2                            Score: 11     Queries matched: 1
 CONTIG=Contig10254 POSCDS1=99221 POSCDS2=101809
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   495.30   988.58   989.55   -0.97 0  11  7.5 5  K.NNLLEFLK.S

 
      Proteins matching the same set of peptides:
      20309.1                           Score: 11     Queries matched: 1
12.   13398.2                           Score: 10     Queries matched: 1
 CONTIG=Contig20014 POSCDS1=39804 POSCDS2=42866
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   495.30   988.58   988.56   0.03 0  10  9.2 7  K.SLNSQSLIK.L

 
      Proteins matching the same set of peptides:
      19358.2                           Score: 10     Queries matched: 1
13.   18977.1                           Score: 10     Queries matched: 1
 CONTIG=Contig10152 POSCDS1=1048 POSCDS2=1380
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   497.76   993.51   994.49   -0.98 0  10  7.8 2  -.MSDLINFR.I

 
      Proteins matching the same set of peptides:
      24717.1                           Score: 10     Queries matched: 1
14.   10160.1                           Score: 10     Queries matched: 1
 CONTIG=Contig10212 POSCDS1=115588 POSCDS2=115980
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   495.30   988.58   987.53   1.05 1  10  9.8 8  K.NENIVGKSK.L

 
      Proteins matching the same set of peptides:
      22646.1                           Score: 10     Queries matched: 1
15.   23344.1                           Mass: 15135    Score: 9      Queries matched: 1
 CONTIG=Contig20194 POSCDS1=172045 POSCDS2=172410
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
18   855.41   1708.81   1708.98   -0.17 1  9  5.5 1  R.YIYITLVALKEIDR.R

16.   6816.1                            Mass: 45848    Score: 9      Queries matched: 1
 CONTIG=Contig10230 POSCDS1=131424 POSCDS2=132680
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   463.74   925.47   925.40   0.07 1  9  8.9 1  R.GDKGDFCK.S

 
      Proteins matching the same set of peptides:
      21596.1                           Mass: 46246    Score: 9      Queries matched: 1
 CONTIG=Contig20230 POSCDS1=131718 POSCDS2=132974
17.   11273.2                           Score: 8      Queries matched: 1
 CONTIG=Contig20223 POSCDS1=29752 POSCDS2=30987
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   497.76   993.51   992.54   0.97 1  8  12 3  K.FWKNISAK.S

 
      Proteins matching the same set of peptides:
      18148.2                           Score: 8      Queries matched: 1
18.   7525.1                            Score: 8      Queries matched: 1
 CONTIG=Contig10209 POSCDS1=14882 POSCDS2=15388
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   495.30   988.58   988.53   0.05 0  8  15 9  K.ISTHAVSFK.L

 
      Proteins matching the same set of peptides:
      22725.1                           Score: 8      Queries matched: 1
19.   14262.2                           Score: 8      Queries matched: 1
 CONTIG=Contig10218 POSCDS1=118253 POSCDS2=121198
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   495.30   988.58   988.52   0.06 1  8  15 10  R.IRSEDEIK.R

 
      Proteins matching the same set of peptides:
      22110.1                           Score: 8      Queries matched: 1
20.   15236.2                           Score: 7      Queries matched: 1
 CONTIG=Contig10163 POSCDS1=1921 POSCDS2=4413
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   577.33   1152.65   1152.60   0.05 0  7  14 2  K.IIYCTGITGR.L

 
      Proteins matching the same set of peptides:
      24250.1                           Score: 7      Queries matched: 1
Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
16   806.36   1610.71   1610.82   -0.10 0  6  11 1  EATPDVQQVAETVPK
12   716.88   1431.74   1431.79   -0.05 0  2  35 1  ILSICFLNPISR
22   664.00   1988.98   1988.98   -0.01 0  1  31 1  EFDSFNNDLILNPNIPK
9   594.30   1186.59   1185.54   1.05 0  1  61 1  NSLNEFSGYR
5   532.32   1062.62        
6   573.83   1145.65        
8   584.82   1167.62        
14   755.39   1508.76        
15   797.90   1593.79        
17   815.38   1628.75        
20   984.02   1966.03        
23   1010.49   2018.96        
24   1052.04   2102.07        
25   1082.07   2162.12        
27   758.74   2273.21        
28   850.76   2549.27        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 28

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 15 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  9.3    0.07   16  6816.1 R.GDKGDFCK.S
  1.7   -1.08              K.NNLLLVNK.A
Top scoring peptide matches to query 2
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 63.2    0.04    1  mCHT2   K.LSSAIEEIK.C
 18.1   -0.97    7  8495.2  R.NSSLSNLKK.K
 17.7   -0.89    6  20170.1 K.NSIASAGENK.L
 12.2   -0.01    9  14540.2 K.ISKLSNISK.Y
 10.8   -0.97   11  2807.2  K.NNLLEFLK.S
 10.8   -0.95   10  1460.1  K.NNNLQFLK.N
  9.9    0.03   12  13398.2 K.SLNSQSLIK.L
  9.7    1.05   14  10160.1 K.NENIVGKSK.L
  7.9    0.05   18  7525.1  K.ISTHAVSFK.L
  7.7    0.06   19  14262.2 R.IRSEDEIK.R
Top scoring peptide matches to query 3
Score greater than 25 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 42.5    0.02    1  mCHT2   K.FADTLWNK.F
  9.8   -0.98   13  18977.1 MSDLINFR
  8.1    0.97   17  11273.2 K.FWKNISAK.S
  5.9    0.06               K.FEGTEFHK.M
  5.4    1.08               R.VETDDGETK.L
  4.7   -0.02               K.FPQFLTNK.F
  3.2    0.99               K.KDFEDVLK.T
  2.9   -0.07               R.FIWILFR.M
  1.2    1.02               R.EKFENQAK.I
  0.3   -0.00               K.FIGKDETGK.R
Top scoring peptide matches to query 4
Score greater than 19 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 19.0    0.04    1  mCHT2 K.GENFVVQVK.N
  5.7    0.04             K.DANVFVLNK.E
Top scoring peptide matches to query 5
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 6
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 7
Score greater than 22 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 44.2    0.08    3  TRBOTR  K.SSGTSYPDVLK.C
  7.1    0.05   20  15236.2 K.IIYCTGITGR.L
  5.6    0.03               K.SSPLKSYIMK.N
  5.4    1.10               R.SIMSGMVWNK.N
  5.4    1.14               R.SMLSEESNQK.K
  5.0    1.08               -.MVNTTSTSRR.R
  4.4   -0.93               K.YDTKNISWK.Y
  2.6   -0.93               R.RYNSLTSASR.V
  0.7   -0.84               K.EDVASEEYGR.G
Top scoring peptide matches to query 8
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 9
Score greater than 13 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.0    1.05        R.NSLNEFSGYR.D
  0.7    0.07        K.EHQTQTQTQS.-
  0.5   -0.00        R.QSVEGDINGLR.K
Top scoring peptide matches to query 10
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 20.9    0.05    5  mRBT5 IYDQLPECAK
Top scoring peptide matches to query 11
Score greater than 21 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 89.2    0.09    2  mSSR1 -.APPACLLACVAK.V
  3.0    0.03             K.KQVATQNLAAAR.L
Top scoring peptide matches to query 12
Score greater than 15 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.3   -0.05        K.ILSICFLNPISR.H
  2.0   -1.06        R.YIVASQDLQLRK.K
  0.4   -0.07        K.IFNTIGEILEKR.L
Top scoring peptide matches to query 13
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 21.3    0.04    3  TRBOTR K.LQGIVSWGSGCAQK.N
Top scoring peptide matches to query 14
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 15
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 16
Score greater than 16 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  6.3   -0.10        K.EATPDVQQVAETVPK.T
  2.9   -0.13        R.WSLKDLAFEFLSR.R
Top scoring peptide matches to query 17
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 18
Score greater than 22 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  9.3   -0.17   15  23344.1 R.YIYITLVALKEIDR.R
Top scoring peptide matches to query 19
Score greater than 23 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 59.6    0.04    1  mCHT2 K.LFVGVPATSNIAGYVDTSK.L
  7.0    1.12             K.LNTSVYYEYAKAEQMK.S
  2.6    0.02             -.TLPNLSAEQICPVQAGIK.D
  2.1    1.06             K.LALAEQGLNSELYFQNK.N
  0.0   -1.00             M.YELVKVGHDNLVGEVIR.I
Top scoring peptide matches to query 20
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 21
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 31.1    0.01    4  11189.3 K.SVSKSVIEHSVEVLNDVK.G
 16.0    0.96    8  19558.1 K.NLIDKISININQIINDK.Q
  5.8   -0.15               R.FLPSIRNIIVTLLQTLK.T
  4.6   -0.96               R.AGTQVMLKTGHIEQTIDK.E
  2.0   -0.84               R.SSASIPHDSFGYSGSITEK.I
  1.4    1.12               R.SFTNTFVAEAVVNNANNR.G
  1.4   -0.09               K.VVLGSLIFKPAHTLQFAK.N
  1.4    0.03               K.VGEEHFGVSIYYISKIK.S
  0.9   -0.03               K.SQLQQIINLVKESQVNK.A
Top scoring peptide matches to query 22
Score greater than 14 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.3   -0.01        R.EFDSFNNDLILNPNIPK.T
  0.9   -1.07        R.SIVQHRMVCLNPRPQR.S
  0.0    0.91        R.SLLLSVSMEGILNIWDAK.T
Top scoring peptide matches to query 23
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 24
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 25
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 26
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 15.2    0.03    1  mCHT2 K.TVLLSLGGGVGDYGFSDVASATK.F
Top scoring peptide matches to query 27
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 28
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide