Mascot Search Results

User            : 
Email           : 
Search title    : 
MS data file    : cw0252_q3.pkl
Database        : CAlbicans dos (20286 sequences; 6720281 residues)
Timestamp       : 17 Oct 2005 at 14:36:14 GMT
Significant hits: mSSR1       |Candida albicans 212 aa - Secretory Stress Response
                  TRBOTR      trypsin (EC3.4.21.4 ) precursor-bovine
                  mCHT2       583 aa - chitinase 2 precursor Candida albicans
                  mCrh11      |predicted| C. albicans |CA0375|: 453 aa
                  mALS1.5eoc  |predicted| C. albicans |CA0909| 658 aa - agglutinin-like,5-prime end
                  13836.2     CONTIG=Contig10146 POSCDS1=76347 POSCDS2=77504

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 30 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

  Help
  Significance threshold p< Max. number of hits  
  Standard scoring  MudPIT scoring  Ions score cut-off Show sub-sets
  Show pop-ups  Suppress pop-ups  Sort unassigned Require bold red

        Error tolerant   

1.    mSSR1                             Mass: 20690    Score: 275    Queries matched: 5
 |Candida albicans 212 aa - Secretory Stress Response
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   635.84   1269.66   1269.66   0.00 0  58  0.00011 1  -.APPACLLACVAK.V
18   542.94   1625.79   1625.86   -0.07 1  83  2.3e-007 1  -.APPACLLACVAKVEK.G
19   815.41   1628.82   1627.70   1.11 0  47  0.001 1  K.CSGLNDLSCICTTK.N
20   855.38   1708.75   1707.78   0.97 0  43  0.0024 1  K.EICPNGDADTAISAFK.S
22   704.31   2109.90   2108.99   0.91 1  44  0.0014 1  K.CLKEICPNGDADTAISAFK.S

2.    TRBOTR                            Mass: 24662    Score: 209    Queries matched: 3
 trypsin (EC3.4.21.4 ) precursor-bovine
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
10   577.29   1152.56   1152.57   -0.00 0  61  5.3e-005 1  K.SSGTSYPDVLK.C
23   1082.53   2163.05   2162.05   1.00 0  95  1.1e-008 1  R.LGEDNINVVEGNEQFISASK.S
24   758.36   2272.06   2272.15   -0.10 0  53  0.00017 1  K.SIVHPSYNSNTLNNDIMLIK.L

3.    mCHT2                             Mass: 59565    Score: 172    Queries matched: 6
 583 aa - chitinase 2 precursor Candida albicans
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   397.21   792.40   792.38   0.02 0  12  5.9 1  K.TCQSLGK.T
2   425.20   848.39   848.40   -0.02 0  43  0.0055 1  K.FADSAPNK.N
3   425.21   848.40   848.40   -0.00 0  (36) 0.027 1  K.FADSAPNK.N
5   495.29   988.56   988.54   0.01 0  54  0.00033 1  K.LSSAIEEIK.C
6   497.74   993.47   993.49   -0.03 0  34  0.03 1  K.FADTLWNK.F
7   510.29   1018.57   1018.54   0.02 0  29  0.11 1  K.GENFVVQVK.N

 
      Proteins matching the same set of peptides:
      15860.2                           Mass: 61554    Score: 172    Queries matched: 6
 |Cht2| CONTIG=Contig10194 POSCDS1=2376 POSCDS2=4127
      19719.2                           Mass: 51350    Score: 172    Queries matched: 6
 CONTIG=Contig20194 POSCDS1=2710 POSCDS2=4131
4.    mCrh11                            Mass: 44667    Score: 48     Queries matched: 1
 |predicted| C. albicans |CA0375|: 453 aa
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   563.28   1124.54   1124.57   -0.04 0  48  0.001 1  K.SVLVADYSSGK.Q

 
      Proteins matching the same set of peptides:
      14360.2                           Mass: 46818    Score: 48     Queries matched: 1
 |Crh11| CONTIG=Contig10158 POSCDS1=92919 POSCDS2=94280
      24611.1                           Mass: 47890    Score: 48     Queries matched: 1
 CONTIG=Contig20158 POSCDS1=86016 POSCDS2=87377
5.    mALS1.5eoc                        Mass: 69052    Score: 44     Queries matched: 1
 |predicted| C. albicans |CA0909| 658 aa - agglutinin-like,5-prime end
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
13   708.33   1414.64   1414.67   -0.03 0  44  0.0025 1  K.STVDPSGYLYASR.V

 
      Proteins matching the same set of peptides:
      13002.2                           Mass: 133694   Score: 44     Queries matched: 1
 |Als1| CONTIG=Contig10233 POSCDS1=72223 POSCDS2=76005
      21498.1                           Mass: 133402   Score: 44     Queries matched: 1
 |Als1| CONTIG=Contig20233 POSCDS1=71475 POSCDS2=75257
6.    13836.2                           Mass: 37190    Score: 32     Queries matched: 2
 CONTIG=Contig10146 POSCDS1=76347 POSCDS2=77504
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   425.20   848.39   847.44   0.95 1  (25) 0.33 2  K.AGKTADSAK.D
 3   425.21   848.40   847.44   0.96 1  32  0.07 2  K.AGKTADSAK.D

 
      Proteins matching the same set of peptides:
      24991.1                           Mass: 37577    Score: 32     Queries matched: 2
 CONTIG=Contig20146 POSCDS1=76357 POSCDS2=77520
7.    14262.2                           Score: 11     Queries matched: 1
 CONTIG=Contig10218 POSCDS1=118253 POSCDS2=121198
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   495.29   988.56   988.52   0.04 1  11  7.8 2  R.IRSEDEIK.R

 
      Proteins matching the same set of peptides:
      22110.1                           Score: 11     Queries matched: 1
8.    23687.1                           Score: 10     Queries matched: 1
 CONTIG=Contig20184 POSCDS1=49345 POSCDS2=49773
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 9   563.28   1124.54   1124.79   -0.25 1  10  5.6 2  K.VSLLKVLLLK.L

 
      Proteins matching the same set of peptides:
      28713.1                           Score: 10     Queries matched: 1
9.    6749.2                            Score: 10     Queries matched: 1
 CONTIG=Contig10046 POSCDS1=148294 POSCDS2=149190
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   510.29   1018.57   1019.42   -0.85 0  10  8.7 2  K.WESAGEVDQ.-

 
      Proteins matching the same set of peptides:
      27025.1                           Score: 10     Queries matched: 1
10.   20568.1                           Mass: 7455     Score: 10     Queries matched: 1
 CONTIG=Contig20248 POSCDS1=139219 POSCDS2=139419
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   451.66   901.31   902.49   -1.19 1  10  9.3 1  R.RQQSSGIK.N

 
      Proteins matching the same set of peptides:
      27685.1                           Mass: 7455     Score: 10     Queries matched: 1
 CONTIG=Contig10248 POSCDS1=146655 POSCDS2=146855
11.   8884.2                            Score: 10     Queries matched: 1
 CONTIG=Contig10176 POSCDS1=47092 POSCDS2=54798
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   510.29   1018.57   1018.54   0.02 0  10  9.6 3  K.DANVFVLNK.E

 
      Proteins matching the same set of peptides:
      23847.1                           Score: 10     Queries matched: 1
12.   13152.2                           Score: 9      Queries matched: 1
 CONTIG=Contig10166 POSCDS1=40346 POSCDS2=40987
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   510.29   1018.57   1017.51   1.06 1  9  11 4  K.SDNKNVVDK.T

 
      Proteins matching the same set of peptides:
      17498.2                           Score: 9      Queries matched: 1
13.   20170.1                           Score: 9      Queries matched: 1
 CONTIG=Contig10053 POSCDS1=71020 POSCDS2=71511
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   495.29   988.56   989.48   -0.92 0  9  12 5  K.NSIASAGENK.L

 
      Proteins matching the same set of peptides:
      26819.1                           Score: 9      Queries matched: 1
14.   3370.2                            Score: 8      Queries matched: 1
 CONTIG=Contig10194 POSCDS1=235169 POSCDS2=235759
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   510.29   1018.57   1019.60   -1.03 1  8  13 5  K.GVYIKVVDK.D

 
      Proteins matching the same set of peptides:
      23307.1                           Score: 8      Queries matched: 1
15.   13398.2                           Score: 8      Queries matched: 1
 CONTIG=Contig20014 POSCDS1=39804 POSCDS2=42866
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   495.29   988.56   988.56   0.00 0  8  14 6  K.SLNSQSLIK.L

 
      Proteins matching the same set of peptides:
      19358.2                           Score: 8      Queries matched: 1
16.   13268.1                           Score: 8      Queries matched: 1
 CONTIG=Contig10158 POSCDS1=178031 POSCDS2=179503
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   495.29   988.56   989.48   -0.92 1  8  16 7  K.DGSNKEVNK.K

 
      Proteins matching the same set of peptides:
      24610.1                           Score: 8      Queries matched: 1
17.   2807.2                            Score: 8      Queries matched: 1
 CONTIG=Contig10254 POSCDS1=99221 POSCDS2=101809
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   495.29   988.56   989.55   -1.00 0  8  16 8  K.NNLLEFLK.S

 
      Proteins matching the same set of peptides:
      20309.1                           Score: 8      Queries matched: 1
18.   3569.2                            Score: 7      Queries matched: 1
 CONTIG=Contig20057 POSCDS1=76699 POSCDS2=79866
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   495.29   988.56   988.53   0.03 0  9  11 3  R.QMLDILEK.F

 
      Proteins matching the same set of peptides:
      29784.1                           Score: 7      Queries matched: 1
19.   1334.1                            Score: 7      Queries matched: 1
 CONTIG=Contig10247 POSCDS1=74356 POSCDS2=76884
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   495.29   988.56   988.49   0.06 0  7  17 9  K.QTEITAGNR.V

 
      Proteins matching the same set of peptides:
      20707.1                           Score: 7      Queries matched: 1
20.   12253.1                           Score: 7      Queries matched: 1
 CONTIG=Contig20215 POSCDS1=286847 POSCDS2=287659
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   425.20   848.39   847.39   0.99 0  7  21 3  K.AGETVDEK.L

 
      Proteins matching the same set of peptides:
      19185.1                           Score: 7      Queries matched: 1
Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   533.25   1064.48   1063.55   0.93 1  6  21 1  SLTGKSESQK
17   806.32   1610.63   1609.95   0.68 1  1  32 1  TGLLDLAKGLVAANVR
12   662.28   1322.55        
14   716.83   1431.65        
15   747.29   1492.57        
16   797.87   1593.73        
21   894.41   1786.81        
25   1178.86   3533.57        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 25

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 16 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 11.8    0.02    3  mCHT2 K.TCQSLGK.T
  2.8   -0.93             K.KCDEDK.V
  1.3   -1.05             K.GYLRTGK.I
Top scoring peptide matches to query 2
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 42.7   -0.02    3  mCHT2   K.FADSAPNK.N
 25.0    0.95    6  13836.2 K.AGKTADSAK.D
  7.0    0.99   20  12253.1 K.AGETVDEK.L
  5.0    0.98               K.GGNVSEASK.I
  3.8   -0.05               R.DKPTFNK.D
  3.7    0.93               K.QFSKPNK.F
  3.4   -0.02               M.PANGSFEK.M
  2.9   -0.05               K.NTIYPNK.F
  2.4    0.91               K.GAAEIIFK.N
  2.1    0.96               K.KTELDDK.N
Top scoring peptide matches to query 3
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 35.8   -0.00    3  mCHT2   K.FADSAPNK.N
 31.6    0.96    6  13836.2 K.AGKTADSAK.D
  6.6   -0.08               -.KQTITMK.K
  5.6   -0.07               R.QEIFGKK.K
  4.6   -0.04               K.NTIYPNK.F
  4.6   -0.01               K.RGDYPNK.Y
  4.3   -0.05               R.QKNFANK.Q
  3.0   -0.00               M.PANGSFEK.M
  2.3    0.95               K.QFSKPNK.F
  1.3    1.00               K.RTEEGEK.W
Top scoring peptide matches to query 4
Score greater than 22 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  9.9   -1.19   10  20568.1 R.RQQSSGIK.N
Top scoring peptide matches to query 5
Score greater than 26 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 54.3    0.01    3  mCHT2   K.LSSAIEEIK.C
 10.6    0.04    7  14262.2 R.IRSEDEIK.R
  9.2    0.03   18  3569.2  R.QMLDILEK.F
  8.9   -0.99               R.NSSLSNLKK.K
  8.6   -0.92   13  20170.1 K.NSIASAGENK.L
  8.2    0.00   15  13398.2 K.SLNSQSLIK.L
  7.6   -0.92   16  13268.1 K.DGSNKEVNK.K
  7.6   -1.00   17  2807.2  K.NNLLEFLK.S
  7.2    0.06   19  1334.1  K.QTEITAGNR.V
  6.7   -0.02               R.SSILTDLIK.Y
Top scoring peptide matches to query 6
Score greater than 17 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 34.0   -0.03    3  mCHT2 K.FADTLWNK.F
  2.5    0.01             K.FEGTEFHK.M
  0.8   -0.02             K.DNGFNKATK.L
  0.8    1.03             -.MSNANQSNK.F
  0.6   -0.07             K.TVATTTTTAK.K
Top scoring peptide matches to query 7
Score greater than 24 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 29.2    0.02    3  mCHT2   K.GENFVVQVK.N
 10.0   -0.85    9  6749.2  K.WESAGEVDQ.-
  9.6    0.02   11  8884.2  K.DANVFVLNK.E
  9.2    1.06   12  13152.2 K.SDNKNVVDK.T
  8.3   -1.03   14  3370.2  K.GVYIKVVDK.D
  6.7   -0.96               K.GEFDVNLVK.K
  4.9    0.01               R.GGFGEVVKAR.S
  4.8   -1.01               K.TWYPVKVK.K
  4.7    0.06               K.QSEDIRGSK.N
  3.1    1.00               R.TTSIQILDK.W
Top scoring peptide matches to query 8
Score greater than 17 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  5.7    0.93        R.SLTGKSESQK.L
  3.9    0.93        K.YEDGINLIK.K
  3.6   -1.09        K.FESEVLSKK.L
  3.1   -0.01        K.STMQELNSR.L
  1.5   -1.08        R.HAHAHTHKK.K
  1.0   -0.03        K.SDTTSLWQK.R
  0.9   -0.04        R.STMEISNVGK.M
  0.5   -1.13        R.TVYDLLKSK.Y
Top scoring peptide matches to query 9
Score greater than 26 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 47.8   -0.04    4  mCrh11  K.SVLVADYSSGK.Q
 10.4   -0.25    8  23687.1 K.VSLLKVLLLK.L
  1.8    1.00               K.EIEDLFGSSK.K
  0.3   -0.05               K.WIFIGSSSTK.W
Top scoring peptide matches to query 10
Score greater than 22 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 61.2   -0.00    2  TRBOTR K.SSGTSYPDVLK.C
  5.7    1.02              R.SIMSGMVWNK.N
  5.7    1.05              R.SMLSEESNQK.K
  3.2   -0.01              R.QMLQSPFFR.Q
  2.3   -0.03              K.SGLFDFQIAR.D
  2.0   -0.06              K.KEIAHTIGER.N
  1.8   -1.08              K.QETAHIISKK.L
  0.1   -0.11              R.LTTSYKTLVK.K
Top scoring peptide matches to query 11
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 57.7    0.00    1  mSSR1 -.APPACLLACVAK.V
Top scoring peptide matches to query 12
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 13
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 43.9   -0.03    5  mALS1.5eoc K.STVDPSGYLYASR.V
Top scoring peptide matches to query 14
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 15
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 16
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 17
Score greater than 14 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.1    0.68        K.TGLLDLAKGLVAANVR.I
Top scoring peptide matches to query 18
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 83.4   -0.07    1  mSSR1 -.APPACLLACVAKVEK.G
Top scoring peptide matches to query 19
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 47.2    1.11    1  mSSR1 K.CSGLNDLSCICTTK.N
Top scoring peptide matches to query 20
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 42.7    0.97    1  mSSR1 K.EICPNGDADTAISAFK.S
Top scoring peptide matches to query 21
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 22
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 44.0    0.91    1  mSSR1 K.CLKEICPNGDADTAISAFK.S
Top scoring peptide matches to query 23
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 95.3    1.00    2  TRBOTR R.LGEDNINVVEGNEQFISASK.S
Top scoring peptide matches to query 24
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 52.9   -0.10    2  TRBOTR K.SIVHPSYNSNTLNNDIMLIK.L
Top scoring peptide matches to query 25
Score greater than 23 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide