Mascot Search Results

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MS data file    : cw0252_q1.pkl
Database        : CAlbicans dos (20286 sequences; 6720281 residues)
Timestamp       : 17 Oct 2005 at 12:55:56 GMT
Significant hits: mCHT2       583 aa - chitinase 2 precursor Candida albicans
                  mSSR1       |Candida albicans 212 aa - Secretory Stress Response
                  TRBOTR      trypsin (EC3.4.21.4 ) precursor-bovine
                  mALS1.5eoc  |predicted| C. albicans |CA0909| 658 aa - agglutinin-like,5-prime end

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 31 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    mCHT2                             Mass: 59565    Score: 276    Queries matched: 8
 583 aa - chitinase 2 precursor Candida albicans
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   397.21   792.41   792.38   0.03 0  26  0.22 1  K.TCQSLGK.T
2   425.22   848.42   848.40   0.02 0  41  0.0086 1  K.FADSAPNK.N
3   495.28   988.56   988.54   0.01 0  66  2e-005 1  K.LSSAIEEIK.C
4   495.28   988.56   988.54   0.01 0  (52) 0.00055 1  K.LSSAIEEIK.C
5   497.76   993.51   993.49   0.02 0  32  0.046 1  K.FADTLWNK.F
6   510.28   1018.55   1018.54   0.01 0  28  0.14 1  K.GENFVVQVK.N
18   968.48   1934.94   1933.90   1.04 0  35  0.013 1  -.SNQVALYWGQNGAGGQER.L
19   970.06   1938.11   1938.01   0.10 0  48  0.00063 1  K.LFVGVPATSNIAGYVDTSK.L

2.    mSSR1                             Mass: 20690    Score: 224    Queries matched: 4
 |Candida albicans 212 aa - Secretory Stress Response
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   635.84   1269.67   1269.66   0.02 0  (56) 0.00017 1  -.APPACLLACVAK.V
12   635.84   1269.67   1269.66   0.02 0  60  5.8e-005 1  -.APPACLLACVAK.V
16   814.88   1627.75   1627.70   0.04 0  93  2.6e-008 1  K.CSGLNDLSCICTTK.N
17   854.91   1707.80   1707.78   0.03 0  71  3.7e-006 1  K.EICPNGDADTAISAFK.S

3.    TRBOTR                            Mass: 24662    Score: 130    Queries matched: 2
 trypsin (EC3.4.21.4 ) precursor-bovine
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
22   1082.07   2162.13   2162.05   0.09 0  93  1.6e-008 1  R.LGEDNINVVEGNEQFISASK.S
23   758.40   2272.17   2272.15   0.02 0  36  0.008 1  K.SIVHPSYNSNTLNNDIMLIK.L

4.    mALS1.5eoc                        Mass: 69052    Score: 55     Queries matched: 2
 |predicted| C. albicans |CA0909| 658 aa - agglutinin-like,5-prime end
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   561.79   1121.56   1120.55   1.00 0  15  2.1 1  K.TCTSNGIQIK.Y
13   708.35   1414.68   1414.67   0.01 0  39  0.007 1  K.STVDPSGYLYASR.V

 
      Proteins matching the same set of peptides:
      13002.2                           Mass: 133694   Score: 55     Queries matched: 2
 |Als1| CONTIG=Contig10233 POSCDS1=72223 POSCDS2=76005
      21498.1                           Mass: 133402   Score: 55     Queries matched: 2
 |Als1| CONTIG=Contig20233 POSCDS1=71475 POSCDS2=75257
5.    13836.2                           Score: 27     Queries matched: 1
 CONTIG=Contig10146 POSCDS1=76347 POSCDS2=77504
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   425.22   848.42   847.44   0.98 1  27  0.18 2  K.AGKTADSAK.D

 
      Proteins matching the same set of peptides:
      24991.1                           Score: 27     Queries matched: 1
6.    20170.1                           Mass: 17760    Score: 19     Queries matched: 2
 CONTIG=Contig10053 POSCDS1=71020 POSCDS2=71511
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.28   988.56   989.48   -0.92 0  19  1.1 2  K.NSIASAGENK.L
 4   495.28   988.56   989.48   -0.92 0  (10) 9 3  K.NSIASAGENK.L

 
      Proteins matching the same set of peptides:
      26819.1                           Mass: 17760    Score: 19     Queries matched: 2
 CONTIG=Contig20053 POSCDS1=70977 POSCDS2=71468
7.    mIPF8527|mPga29:                  Mass: 19928    Score: 18     Queries matched: 1
 189 aa - Candida albicans unknown function
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   554.33   1106.64   1106.63   0.00 0  18  1.3 1  -.TISSIQLFAK.S

8.    14262.2                           Score: 16     Queries matched: 3
 CONTIG=Contig10218 POSCDS1=118253 POSCDS2=121198
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.28   988.56   988.52   0.04 1  10  8.1 7  R.IRSEDEIK.R
 4   495.28   988.56   988.52   0.04 1  (9) 12 5  R.IRSEDEIK.R
 7   547.29   1092.57   1092.50   0.06 0  5  23 2  K.SACWTNNIK.K

 
      Proteins matching the same set of peptides:
      22110.1                           Score: 16     Queries matched: 3
9.    2807.2                            Score: 13     Queries matched: 2
 CONTIG=Contig10254 POSCDS1=99221 POSCDS2=101809
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.28   988.56   989.55   -1.00 0  13  4.1 4  K.NNLLEFLK.S
 4   495.28   988.56   989.55   -1.00 0  (7) 18 8  K.NNLLEFLK.S

 
      Proteins matching the same set of peptides:
      20309.1                           Score: 13     Queries matched: 2
10.   8495.2                            Score: 13     Queries matched: 2
 CONTIG=Contig10163 POSCDS1=196876 POSCDS2=198465
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.28   988.56   989.55   -1.00 1  15  2.8 3  R.NSSLSNLKK.K
 4   495.28   988.56   989.55   -1.00 1  (9) 11 4  R.NSSLSNLKK.K

 
      Proteins matching the same set of peptides:
      24293.1                           Score: 13     Queries matched: 2
11.   18628.1                           Score: 13     Queries matched: 1
 CONTIG=Contig10216 POSCDS1=12957 POSCDS2=14267
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   510.28   1018.55   1018.53   0.02 1  13  4.5 2  K.SAIDDVKSGK.V

 
      Proteins matching the same set of peptides:
      19869.2                           Score: 13     Queries matched: 1
12.   8884.2                            Mass: 296514   Score: 12     Queries matched: 1
 CONTIG=Contig10176 POSCDS1=47092 POSCDS2=54798
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   510.28   1018.55   1018.54   0.01 0  12  5.8 3  K.DANVFVLNK.E

 
      Proteins matching the same set of peptides:
      23847.1                           Mass: 298487   Score: 12     Queries matched: 1
 CONTIG=Contig20176 POSCDS1=47004 POSCDS2=54761
13.   1460.1                            Score: 11     Queries matched: 1
 CONTIG=Contig10237 POSCDS1=76798 POSCDS2=79839
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.28   988.56   989.53   -0.97 0  11  7.8 5  K.NNNLQFLK.N

 
      Proteins matching the same set of peptides:
      21148.1                           Score: 11     Queries matched: 1
14.   13268.1                           Mass: 57351    Score: 11     Queries matched: 2
 CONTIG=Contig10158 POSCDS1=178031 POSCDS2=179503
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.28   988.56   989.48   -0.92 1  11  8 6  K.DGSNKEVNK.K
 4   495.28   988.56   989.48   -0.92 1  (8) 13 6  K.DGSNKEVNK.K

 
      Proteins matching the same set of peptides:
      24610.1                           Mass: 60756    Score: 11     Queries matched: 2
 CONTIG=Contig20158 POSCDS1=171085 POSCDS2=172557
15.   10160.1                           Score: 10     Queries matched: 1
 CONTIG=Contig10212 POSCDS1=115588 POSCDS2=115980
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.28   988.56   987.53   1.02 1  10  9.9 8  K.NENIVGKSK.L

 
      Proteins matching the same set of peptides:
      22646.1                           Score: 10     Queries matched: 1
16.   16861.2                           Score: 9      Queries matched: 1
 CONTIG=Contig10155 POSCDS1=36781 POSCDS2=37440
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.28   988.56   988.58   -0.03 0  9  10 9  R.SSILTDLIK.Y

 
      Proteins matching the same set of peptides:
      24694.1                           Score: 9      Queries matched: 1
17.   7414.1                            Score: 9      Queries matched: 1
 CONTIG=Contig10212 POSCDS1=206668 POSCDS2=209133
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.28   988.56   989.46   -0.90 0  9  12 10  K.NNECLVNK.G

 
      Proteins matching the same set of peptides:
      22550.1                           Score: 9      Queries matched: 1
18.   793.1                             Score: 8      Queries matched: 1
 CONTIG=Contig10225 POSCDS1=88407 POSCDS2=93209
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   495.28   988.56   988.61   -0.05 1  10  8.5 2  K.IRLFALEK.V

 
      Proteins matching the same set of peptides:
      21886.1                           Score: 8      Queries matched: 1
19.   13398.2                           Score: 8      Queries matched: 1
 CONTIG=Contig20014 POSCDS1=39804 POSCDS2=42866
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   495.28   988.56   988.56   -0.00 0  8  14 7  K.SLNSQSLIK.L

 
      Proteins matching the same set of peptides:
      19358.2                           Score: 8      Queries matched: 1
20.   21423.1                           Score: 8      Queries matched: 1
 CONTIG=Contig20234 POSCDS1=92886 POSCDS2=93365
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   510.28   1018.55   1017.53   1.02 0  8  15 4  K.GQTEIETIK.D

 
      Proteins matching the same set of peptides:
      27914.1                           Score: 8      Queries matched: 1
Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   547.29   1092.57   1092.58   -0.02 0  6  22 1  TLYQDVGLGK
20   974.49   1946.97   1947.03   -0.06 1  5  16 1  QVIDEKQDLQQYLLSK
10   626.31   1250.61   1251.63   -1.02 1  4  28 1  DMKQYIVAER
15   806.36   1610.70   1610.85   -0.14 1  1  40 1  WSLKDLAFEFLSR
14   792.93   1583.85        
21   677.67   2029.99        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 23

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 21 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 26.0    0.03    1  mCHT2 K.TCQSLGK.T
  7.3    0.05             K.TTQSNDK.E
  5.6   -0.04             K.KVFTNGK.V
  4.0   -0.07             K.TRIYLK.G
  3.9   -0.91             K.KCDEDK.V
  3.2    0.02             K.CTQTRK.T
  3.1   -1.02             K.VYQEKK.R
  2.9    0.99             K.MESKLGK.I
  2.9    0.00             K.TGLGNFGK.K
  2.7    1.00             R.FIGVNDK.Y
Top scoring peptide matches to query 2
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 40.8    0.02    1  mCHT2   K.FADSAPNK.N
 27.5    0.98    5  13836.2 K.AGKTADSAK.D
  6.6   -0.02               K.NTIYPNK.F
  6.6    0.01               K.RGDYPNK.Y
  4.7    0.98               K.QEQTSKK.E
  3.6    0.97               K.QFSKPNK.F
  3.2    1.02               K.DQNKDTK.V
  1.6    0.02               K.ISWSDNK.D
  0.8   -0.06               -.KQTITMK.K
  0.6    0.95               K.VRPWYK.W
Top scoring peptide matches to query 3
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 66.5    0.01    1  mCHT2   K.LSSAIEEIK.C
 19.1   -0.92    6  20170.1 K.NSIASAGENK.L
 15.1   -1.00   10  8495.2  R.NSSLSNLKK.K
 13.4   -1.00    9  2807.2  K.NNLLEFLK.S
 10.7   -0.97   13  1460.1  K.NNNLQFLK.N
 10.5   -0.92   14  13268.1 K.DGSNKEVNK.K
 10.5    0.04    8  14262.2 R.IRSEDEIK.R
  9.6    1.02   15  10160.1 K.NENIVGKSK.L
  9.4   -0.03   16  16861.2 R.SSILTDLIK.Y
  8.7   -0.90   17  7414.1  K.NNECLVNK.G
Top scoring peptide matches to query 4
Score greater than 26 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 52.2    0.01    1  mCHT2   K.LSSAIEEIK.C
 10.3   -0.05   18  793.1   K.IRLFALEK.V
 10.0   -0.92    6  20170.1 K.NSIASAGENK.L
  9.2   -1.00   10  8495.2  R.NSSLSNLKK.K
  8.7    0.04    8  14262.2 R.IRSEDEIK.R
  8.3   -0.92   14  13268.1 K.DGSNKEVNK.K
  8.2   -0.00   19  13398.2 K.SLNSQSLIK.L
  6.9   -1.00    9  2807.2  K.NNLLEFLK.S
  6.7    0.06               K.QTEITAGNR.V
  5.8    0.02               K.MKLQSGTPK.R
Top scoring peptide matches to query 5
Score greater than 17 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 32.0    0.02    1  mCHT2 K.FADTLWNK.F
  2.3    1.03             R.ADGKTSAESK.D
  2.1    1.07             -.MSNANQSNK.F
  1.6    0.05             K.FEGTEFHK.M
Top scoring peptide matches to query 6
Score greater than 27 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 27.9    0.01    1  mCHT2   K.GENFVVQVK.N
 12.9    0.02   11  18628.1 K.SAIDDVKSGK.V
 11.8    0.01   12  8884.2  K.DANVFVLNK.E
  7.6    1.02   20  21423.1 K.GQTEIETIK.D
  6.0   -0.01               K.TESVTKQVK.N
  5.6   -0.98               K.GEFDVNLVK.K
  5.1   -0.00               R.GGFGEVVKAR.S
  5.1    0.06               K.DTKGQEDVK.L
  4.1   -1.03               K.TWYPVKVK.K
  3.8    0.95               R.ITIMSVKAR.K
Top scoring peptide matches to query 7
Score greater than 17 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  5.6   -0.02               K.TLYQDVGLGK.V
  5.5    0.06    8  14262.2 K.SACWTNNIK.K
  4.6   -1.00               R.NSELYDLIK.S
  4.1   -0.99               K.MQFLSNNLK.N
  3.7    0.98               K.AEEFKDIIK.I
  1.4    0.98               K.KVYETPEVK.E
  1.1    0.98               K.EQYAIEVLK.I
  1.0    0.01               R.AQYDELARK.N
  0.6   -0.04               K.TLIDLLYDK.G
  0.4   -0.03               K.KYNNNTLVK.E
Top scoring peptide matches to query 8
Score greater than 21 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein          Peptide
 17.5    0.00    7  mIPF8527|mPga29: -.TISSIQLFAK.S
  7.6   -0.92                        K.YSINQLDGAK.S
  4.4    1.09                        K.SVTNVDCALK.N
  3.8    0.08                        R.FILAGEDNTK.N
  3.4    0.06                        R.TSNAKLMQSK.N
  2.8   -0.92                        R.KAAESLGSSMK.Y
  2.2   -0.88                        K.SKHLMFSVC.-
  2.1    1.05                        R.IGSNLDGKFR.T
  1.9   -1.01                        K.NLHSQRIIK.F
  0.8    1.10                        K.EKMAEELEK.L
Top scoring peptide matches to query 9
Score greater than 20 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 15.4    1.00    4  mALS1.5eoc K.TCTSNGIQIK.Y
  6.2    0.01                  R.ASSGNQKSTSR.S
  3.0    0.08                  K.EEEKEDESK.S
  1.7    0.02                  M.MSTSNQVLDK.I
Top scoring peptide matches to query 10
Score greater than 15 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.9   -1.02        K.DMKQYIVAER.E
  2.5   -1.02        K.EEVSKISDFAK.Q
  2.0   -0.02        K.TTETAKAAETTK.A
  1.8   -1.04        K.FVCVCVIISR.D
  0.2   -0.05        K.QQQTTTYIIR.F
Top scoring peptide matches to query 11
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 55.8    0.02    2  mSSR1 -.APPACLLACVAK.V
Top scoring peptide matches to query 12
Score greater than 18 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 60.4    0.02    2  mSSR1 -.APPACLLACVAK.V
  1.6   -0.04             K.KQVATQNLAAAR.L
Top scoring peptide matches to query 13
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 39.4    0.01    4  mALS1.5eoc K.STVDPSGYLYASR.V
Top scoring peptide matches to query 14
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 15
Score greater than 13 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  0.8   -0.14        R.WSLKDLAFEFLSR.R
  0.3    1.01        R.NGIMDPNSFTEEEK.K
Top scoring peptide matches to query 16
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 92.7    0.04    2  mSSR1 K.CSGLNDLSCICTTK.N
Top scoring peptide matches to query 17
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 71.1    0.03    2  mSSR1 K.EICPNGDADTAISAFK.S
Top scoring peptide matches to query 18
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 35.1    1.04    1  mCHT2 -.SNQVALYWGQNGAGGQER.L
Top scoring peptide matches to query 19
Score greater than 18 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 48.0    0.10    1  mCHT2 K.LFVGVPATSNIAGYVDTSK.L
  2.8   -0.88             K.QQCQQLSQEIAKHTIK.L
Top scoring peptide matches to query 20
Score greater than 17 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  4.5   -0.06        K.QVIDEKQDLQQYLLSK.I
  3.9   -1.09        K.IVGTRTDVNEIYAIGSIK.E
  0.3    1.12        K.SSSNISIHPSSSDDDSSPK.T
Top scoring peptide matches to query 21
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 22
Score greater than 20 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 93.4    0.09    3  TRBOTR R.LGEDNINVVEGNEQFISASK.S
  2.1   -0.03              K.IVSVKDNLGLVMFAVSDVEK.C
  1.9    1.12              M.GLSNDQSPLLGGDSLTCWNK.K
Top scoring peptide matches to query 23
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 36.4    0.02    3  TRBOTR K.SIVHPSYNSNTLNNDIMLIK.L