Mascot Search Results

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MS data file    : cw0246_2.pkl
Database        : CAlbicans dos (20286 sequences; 6720281 residues)
Timestamp       : 11 Oct 2005 at 16:12:35 GMT
Significant hits: 6365.2  CONTIG=Contig10051 POSCDS1=935 POSCDS2=2311
                  8215.2  CONTIG=Contig10227 POSCDS1=17731 POSCDS2=20418
                  2353.1  CONTIG=Contig10262 POSCDS1=40829 POSCDS2=41236

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 34 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    6365.2                            Mass: 50410    Score: 73     Queries matched: 5
 CONTIG=Contig10051 POSCDS1=935 POSCDS2=2311
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   426.26   850.51   850.44   0.07 0  3  77 10  K.FDTLVEK.I
4   513.34   1024.66   1024.60   0.05 0  (53) 0.00074 1  K.IGGIGTVPVGR.V
5   513.34   1024.66   1024.60   0.05 0  65  4.4e-005 1  K.IGGIGTVPVGR.V
12   568.36   1134.70   1134.64   0.06 1  8  23 1  K.YAWVLDKLK.A
 13   568.36   1134.70   1134.64   0.06 1  (0) 1.4e+002 3  K.YAWVLDKLK.A

 
      Proteins matching the same set of peptides:
      22137.1                           Mass: 50636    Score: 73     Queries matched: 5
 CONTIG=Contig20218 POSCDS1=144186 POSCDS2=145562
      26916.1                           Mass: 50610    Score: 73     Queries matched: 5
 CONTIG=Contig20051 POSCDS1=935 POSCDS2=2311
      28153.1                           Mass: 50636    Score: 73     Queries matched: 5
 CONTIG=Contig10218 POSCDS1=144463 POSCDS2=145839

2.    8215.2                            Mass: 98267    Score: 63     Queries matched: 2
 CONTIG=Contig10227 POSCDS1=17731 POSCDS2=20418
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   522.34   1042.66   1042.61   0.05 0  65  5.9e-005 1  K.TLANSALVVR.N
7   522.34   1042.66   1042.61   0.05 0  (60) 0.00017 1  K.TLANSALVVR.N

 
      Proteins matching the same set of peptides:
      21692.1                           Mass: 98368    Score: 63     Queries matched: 2
 CONTIG=Contig20227 POSCDS1=17718 POSCDS2=20405
3.    2353.1                            Mass: 15995    Score: 43     Queries matched: 2
 CONTIG=Contig10262 POSCDS1=40829 POSCDS2=41236
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   508.82   1015.62   1015.56   0.06 0  45  0.0056 1  K.IPAWFLNR.Q
3   508.82   1015.62   1015.56   0.06 0  (45) 0.0065 1  K.IPAWFLNR.Q

4.    4281.2                            Mass: 23254    Score: 21     Queries matched: 1
 CONTIG=Contig10053 POSCDS1=137830 POSCDS2=138459
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
10   541.85   1081.69   1082.61   -0.92 1  21  1.1 1  R.TALKLQDPR.I + Acetyl (K)

 
      Proteins matching the same set of peptides:
      26808.1                           Mass: 27151    Score: 21     Queries matched: 1
 CONTIG=Contig20053 POSCDS1=138178 POSCDS2=138912
5.    10590.2                           Score: 13     Queries matched: 2
 CONTIG=Contig10105 POSCDS1=15019 POSCDS2=17814
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   513.34   1024.66   1024.54   0.12 1  13  7 2  K.IGKWYFK.D + 2 Acetyl (K)
 5   513.34   1024.66   1024.54   0.12 1  (12) 9 4  K.IGKWYFK.D + 2 Acetyl (K)

 
      Proteins matching the same set of peptides:
      26042.1                           Score: 13     Queries matched: 2
6.    13904.3                           Score: 12     Queries matched: 1
 CONTIG=Contig10035 POSCDS1=60377 POSCDS2=61150
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   513.34   1024.66   1024.66   -0.01 1  12  8.2 2  R.LGKLNVIVK.Y + Acetyl (K)

 
      Proteins matching the same set of peptides:
      27174.1                           Score: 12     Queries matched: 1
7.    6284.1                            Score: 12     Queries matched: 1
 CONTIG=Contig10137 POSCDS1=97226 POSCDS2=98647
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   513.34   1024.66   1024.57   0.09 1  12  9 3  K.IGYEKFLR.V

 
      Proteins matching the same set of peptides:
      25370.1                           Score: 12     Queries matched: 1
8.    11901.2                           Mass: 20419    Score: 12     Queries matched: 1
 CONTIG=Contig10200 POSCDS1=38996 POSCDS2=39526
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   426.26   850.51   849.44   1.06 0  12  11 1  R.SSSILSEK.I

 
      Proteins matching the same set of peptides:
      23104.1                           Mass: 20789    Score: 12     Queries matched: 1
 CONTIG=Contig20200 POSCDS1=39191 POSCDS2=39730
9.    5002.2                            Mass: 20469    Score: 11     Queries matched: 1
 CONTIG=Contig10206 POSCDS1=32724 POSCDS2=33251
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   513.34   1024.66   1024.59   0.06 0  11  9.9 5  K.ILLEEVGPR.R

 
      Proteins matching the same set of peptides:
      20507.1                           Score: 11     Queries matched: 1
10.   19343.2                           Mass: 14921    Score: 11     Queries matched: 2
 CONTIG=Contig20223 POSCDS1=20268 POSCDS2=20603
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   522.34   1042.66   1043.65   -0.99 0  (11) 13 3  -.LNFLAIVVR.G
 7   522.34   1042.66   1043.65   -0.99 0  11  13 2  -.LNFLAIVVR.G

11.   12079.1                           Score: 11     Queries matched: 1
 CONTIG=Contig10254 POSCDS1=229640 POSCDS2=232294
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   522.34   1042.66   1042.54   0.12 0  11  14 3  K.GLDNLQEVR.D

 
      Proteins matching the same set of peptides:
      20305.1                           Score: 11     Queries matched: 1
12.   24794.1                           Score: 10     Queries matched: 2
 CONTIG=Contig20151 POSCDS1=73861 POSCDS2=74316
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   541.85   1081.69   1080.59   1.10 1  10  12 3  K.EITNKGLHK.V + Acetyl (K)
 11   541.85   1081.69   1080.59   1.10 1  (6) 32 5  K.EITNKGLHK.V + Acetyl (K)

 
      Proteins matching the same set of peptides:
      29061.1                           Score: 10     Queries matched: 2
13.   14407.1                           Score: 10     Queries matched: 1
 CONTIG=Contig10173 POSCDS1=90013 POSCDS2=90762
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   513.34   1024.66   1024.64   0.02 1  10  13 6  K.GIVASPVVKR.L

 
      Proteins matching the same set of peptides:
      23952.1                           Score: 10     Queries matched: 1
14.   8073.2                            Score: 10     Queries matched: 1
 CONTIG=Contig10187 POSCDS1=19810 POSCDS2=21699
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   426.26   850.51   849.43   1.07 0  10  15 2  K.QFEIASR.L

 
      Proteins matching the same set of peptides:
      23570.1                           Score: 10     Queries matched: 1
15.   21118.1                           Score: 10     Queries matched: 2
 CONTIG=Contig20237 POSCDS1=175273 POSCDS2=175686
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   522.34   1042.66   1042.61   0.05 1  10  17 4  K.SVVNKLSVR.L + Acetyl (K)
 7   522.34   1042.66   1042.61   0.05 1  (8) 26 6  K.SVVNKLSVR.L + Acetyl (K)

 
      Proteins matching the same set of peptides:
      27832.1                           Score: 10     Queries matched: 2
16.   25638.1                           Score: 10     Queries matched: 2
 CONTIG=Contig20123 POSCDS1=59684 POSCDS2=60814
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   522.34   1042.66   1042.64   0.03 1  10  18 5  K.KTGLSALIAK.S + Acetyl (K)
 7   522.34   1042.66   1042.64   0.03 1  (8) 26 5  K.KTGLSALIAK.S + Acetyl (K)

 
      Proteins matching the same set of peptides:
      4255.2                            Score: 8      Queries matched: 2
17.   1574.1                            Mass: 155634   Score: 9      Queries matched: 2
 CONTIG=Contig10080 POSCDS1=105034 POSCDS2=109140
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   541.85   1081.69   1080.56   1.13 1  12  8.1 2  K.NKGIDLDHK.R + Acetyl (K)
11   541.85   1081.69   1080.56   1.13 1  (9) 18 1  K.NKGIDLDHK.R + Acetyl (K)

 
      Proteins matching the same set of peptides:
      26364.1                           Mass: 163629   Score: 9      Queries matched: 2
 CONTIG=Contig20080 POSCDS1=104903 POSCDS2=109207
18.   11558.1                           Score: 9      Queries matched: 2
 CONTIG=Contig10151 POSCDS1=52933 POSCDS2=53124
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   522.34   1042.66   1043.57   -0.91 1  (9) 22 6  K.NTTNKITPR.T
 7   522.34   1042.66   1043.57   -0.91 1  9  22 4  K.NTTNKITPR.T

 
      Proteins matching the same set of peptides:
      24742.1                           Score: 9      Queries matched: 2
19.   3566.1                            Score: 9      Queries matched: 1
 CONTIG=Contig10057 POSCDS1=74168 POSCDS2=74395
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   513.34   1024.66   1024.60   0.06 0  9  18 3  R.LNIVYLYK.K

 
      Proteins matching the same set of peptides:
      26689.1                           Score: 9      Queries matched: 1
20.   10134.2                           Score: 9      Queries matched: 1
 CONTIG=Contig10051 POSCDS1=47962 POSCDS2=49410
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   513.34   1024.66   1024.52   0.13 0  9  18 3  K.LNIDFFEK.L

 
      Proteins matching the same set of peptides:
      17908.2                           Score: 9      Queries matched: 1
      23741.1                           Score: 9      Queries matched: 1
      26915.1                           Score: 9      Queries matched: 1
Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   537.33   1072.65   1072.58   0.06 1  7  34 1  IQPWFGKR + Acetyl (K)
8   537.33   1072.65   1072.58   0.06 1  6  43 1  IQPWFGKR + Acetyl (K)
14   545.24   1632.69   1632.84   -0.15 1  5  35 1  VEKYRPDSLEEVK + Acetyl (K)
13   568.36   1134.70   1134.58   0.13 1  4  64 1  SKVDSDTITK + Acetyl (K)
15   713.40   2137.17        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Acetyl (K)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 15

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 23 indicates homology
Score greater than 35 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 11.6    1.06    8  11901.2 R.SSSILSEK.I
 10.1    1.07   14  8073.2  K.QFEIASR.L
  7.8    1.09               K.SSSEKNAK.F
  6.0    0.10               K.FDELAEK.L
  4.8    0.07               K.ITPMSFR.N
  4.2    0.05               R.KFSDNLK.F
  3.7   -0.93               K.EVYLNSK.R
  3.5    0.05               K.QFKAETK.E
  3.5    1.12               K.QFDAAEK.A
  3.2    0.07    1  6365.2  K.FDTLVEK.I
Top scoring peptide matches to query 2
Score greater than 20 indicates homology
Score greater than 35 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 45.3    0.06    3  2353.1 K.IPAWFLNR.Q
  4.8    0.02              K.IGFKPIWR.I
  4.7   -0.98              K.LFLEILNR.C
  4.6    1.09              K.EDADLALIR.R
  3.1    0.12              K.LANLNDSNR.T
  2.1    0.07              K.TGRSINNVR.E
  1.7    0.07              K.EKDVVEAVK.K
Top scoring peptide matches to query 3
Score greater than 21 indicates homology
Score greater than 35 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 44.6    0.06    3  2353.1 K.IPAWFLNR.Q
  6.0    0.12              K.LANLNDSNR.T
  4.3   -0.92              K.IMAKDIGNR.V
  3.2    1.09              K.EDADLALIR.R
  2.3    1.08              K.EEIKNLNR.D
  1.0   -0.01              R.LKLITEATK.K
  0.9    0.04              K.VLVVGLTCR.I
  0.9    0.09              K.IPVMAKCR.I
  0.3    0.08              K.ITENRIDR.G
Top scoring peptide matches to query 4
Score greater than 29 indicates homology
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 52.7    0.05    1  6365.2  K.IGGIGTVPVGR.V
 13.0    0.12    5  10590.2 K.IGKWYFK.D
  8.8    0.13   20  10134.2 K.LNIDFFEK.L
  8.8    0.06   19  3566.1  R.LNIVYLYK.K
  7.0    0.02               K.LNKINLIR.I
  6.7    1.08               K.LILGFYNGK.T
  6.7    0.13               R.AVGGQPQSPGK.N
  6.4   -0.89               K.KGINPNATK.K
  4.6   -0.91               R.VAGLFVHNK.G
  3.5    0.06               K.LNAKPTKK.W
Top scoring peptide matches to query 5
Score greater than 29 indicates homology
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 65.0    0.05    1  6365.2  K.IGGIGTVPVGR.V
 12.3   -0.01    6  13904.3 R.LGKLNVIVK.Y
 11.9    0.09    7  6284.1  K.IGYEKFLR.V
 11.9    0.12    5  10590.2 K.IGKWYFK.D
 11.4    0.06    9  5002.2  K.ILLEEVGPR.R
 10.2    0.02   13  14407.1 K.GIVASPVVKR.L
  9.1    0.02               K.LNKINLIR.I
  7.4    0.09               K.KQGTLPSPR.V
  6.9   -0.94               K.NKNLIGLGR.T
  5.9    1.08               K.KIGIGHSTK.W
Top scoring peptide matches to query 6
Score greater than 28 indicates homology
Score greater than 35 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 64.7    0.05    2  8215.2  K.TLANSALVVR.N
 11.2    0.04               R.SLATIRVQR.D
 11.2   -0.99   10  19343.2 -.LNFLAIVVR.G
 10.1    0.05   15  21118.1 K.SVVNKLSVR.L
  9.9    0.03   16  25638.1 K.KTGLSALIAK.S
  8.9   -0.91   18  11558.1 K.NTTNKITPR.T
  7.6    0.06               K.TAINSLLPSK.F
  6.3   -0.90               K.SKANKPTSK.S
  6.1   -0.93               K.VSGELALLSR.Q
  6.0   -0.90               K.LSEKDGVVR.T
Top scoring peptide matches to query 7
Score greater than 27 indicates homology
Score greater than 35 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 60.1    0.05    2  8215.2  K.TLANSALVVR.N
 11.3   -0.99   10  19343.2 -.LNFLAIVVR.G
 10.8    0.12   11  12079.1 K.GLDNLQEVR.D
  9.0   -0.91   18  11558.1 K.NTTNKITPR.T
  8.3    0.03   16  25638.1 K.KTGLSALIAK.S
  8.2    0.05   15  21118.1 K.SVVNKLSVR.L
  6.2   -0.86               K.AQVAESSPQK.S
  6.1   -0.93               K.VSGELALLSR.Q
  6.0    0.10               R.ANISIGDLAK.E
  6.0   -0.90               K.LSEKDGVVR.T
Top scoring peptide matches to query 8
Score greater than 18 indicates homology
Score greater than 35 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  5.9    0.06        K.IQPWFGKR.N
  5.4    0.01        R.KNIPLPPPR.G
  2.3    0.07        K.AALVREQMR.E
  1.2    0.02        K.KPDFLKGVK.V
  1.2    0.12        K.ERQEELNR.K
  1.2   -0.90        K.AITQESVNGR.I
  0.9    0.08        K.APVELQNFR.S
Top scoring peptide matches to query 9
Score greater than 14 indicates homology
Score greater than 35 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  7.0    0.06        K.IQPWFGKR.N
  1.4    0.07        K.AALVREQMR.E
  1.4    0.02        K.KPDFLKGVK.V
Top scoring peptide matches to query 10
Score greater than 26 indicates homology
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 20.7   -0.92    4  4281.2  R.TALKLQDPR.I
 12.2    1.13   17  1574.1  K.NKGIDLDHK.R
 10.3    1.10   12  24794.1 K.EITNKGLHK.V
  8.1    0.09               K.KLIVPGDEK.I
  7.0    0.04               R.AVLITLNNPK.K
  7.0    0.04               K.KILTIHTSK.M
  7.0   -0.92               K.WIKQELPK.E
  7.0    1.07               K.QDKVIKPK.N
  6.8    0.09               K.RQGIPINER.K
  5.9    0.08               K.INKVNPDLK.V
Top scoring peptide matches to query 11
Score greater than 21 indicates homology
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  8.6    1.13   17  1574.1  K.NKGIDLDHK.R
  8.1    0.08               K.INKVNPDLK.V
  7.6   -0.91               K.NIGNRPKSR.K
  6.9   -0.91               K.TAKSSPAIPK.T
  6.2    1.10   12  24794.1 K.EITNKGLHK.V
  5.8   -0.92               R.NLGVPDLSLR.D
  3.8    0.09               K.KLIVPGDEK.I
  3.0    0.04               K.NITKVIAPGK.I
  2.7    1.18               R.NIGGFCMLK.-
  2.2    0.09               K.RQGIPINER.K
Top scoring peptide matches to query 12
Score greater than 15 indicates homology
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  8.3    0.06    1  6365.2 K.YAWVLDKLK.A
  2.3    0.13              K.SKVDSDTITK.L
Top scoring peptide matches to query 13
Score greater than 16 indicates homology
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.8    0.13              K.SKVDSDTITK.L
  3.5    0.02              K.VKLHIQSIR.V
  0.5    0.06    1  6365.2 K.YAWVLDKLK.A
Top scoring peptide matches to query 14
Score greater than 14 indicates homology
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  4.8   -0.15        R.VEKYRPDSLEEVK.G
  1.6    0.02        K.QNEGEDGDGDELEVK.K
  1.6    0.89        R.GDIIMFRSPENPEK.L
  1.3   -1.12        K.STDVNHIPSHATVSK.E
  1.3   -0.03        K.QESNDIDNNTNEIK.Q
  1.2   -1.14        K.THKIEEDFVLFSK.I
  0.2   -0.22        K.TANLDKLAELLASFK.S
  0.0    0.86        K.NHTDSIGIQHELVK.K
Top scoring peptide matches to query 15
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide