Mascot Search Results

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MS data file    : cw0240_q4.pkl
Database        : CAlbicans dos (20286 sequences; 6720281 residues)
Timestamp       : 11 Aug 2005 at 14:41:34 GMT
Significant hits: mCHT2  583 aa - chitinase 2 precursor Candida albicans
                  mSSR1  |Candida albicans 212 aa - Secretory Stress Response

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 29 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    mCHT2                             Mass: 59565    Score: 64     Queries matched: 5
 583 aa - chitinase 2 precursor Candida albicans
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
14   647.06   1938.17   1938.01   0.16 0  (6) 8.3 1  K.LFVGVPATSNIAGYVDTSK.L
15   970.10   1938.19   1938.01   0.18 0  13  1.6 1  K.LFVGVPATSNIAGYVDTSK.L
18   738.78   2213.31   2213.12   0.19 0  (22) 0.18 1  K.TVLLSLGGGVGDYGFSDVASATK.F
19   1108.14   2214.26   2213.12   1.14 0  45  0.001 1  K.TVLLSLGGGVGDYGFSDVASATK.F
24   843.15   2526.42   2526.21   0.21 0  5  8.3 1  K.NYFLSAAPQCPYPDASLGDLLSK.V

 
      Proteins matching the same set of peptides:
      15860.2                           Mass: 61554    Score: 64     Queries matched: 5
 |Cht2| CONTIG=Contig10194 POSCDS1=2376 POSCDS2=4127
      19719.2                           Mass: 51350    Score: 64     Queries matched: 5
 CONTIG=Contig20194 POSCDS1=2710 POSCDS2=4131

2.    mSSR1                             Mass: 20690    Score: 39     Queries matched: 1
 |Candida albicans 212 aa - Secretory Stress Response
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
10   814.93   1627.84   1627.70   0.14 0  39  0.0065 1  K.CSGLNDLSCICTTK.N

 
      Proteins matching the same set of peptides:
      3054.1                            Mass: 22993    Score: 39     Queries matched: 1
 |Ssr1| CONTIG=Contig10262 POSCDS1=71490 POSCDS2=72194
3.    mUTR2                             Mass: 49676    Score: 27     Queries matched: 1
 |predicted| C. albicans |CA0605| 470 aa - 1,3-1,4-beta-glucanase
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   803.97   1605.92   1605.78   0.14 0  27  0.11 1  R.IQFSLWPGGDSSNAK.G

 
      Proteins matching the same set of peptides:
      8080.2                            Mass: 52187    Score: 27     Queries matched: 1
 |Utr2| CONTIG=Contig20123 POSCDS1=148155 POSCDS2=149567
      29345.1                           Mass: 54908    Score: 27     Queries matched: 1
 CONTIG=Contig10123 POSCDS1=148333 POSCDS2=149745
4.    15898.1                           Mass: 39582    Score: 24     Queries matched: 1
 |Scw1| CONTIG=Contig20125 POSCDS1=83941 POSCDS2=85077
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   790.97   1579.93   1579.79   0.14 0  24  0.2 1  R.LYGVDCSQVEAVLK.A

 
      Proteins matching the same set of peptides:
      m15898.1|mSCW1:                   Mass: 35901    Score: 24     Queries matched: 1
 346 aa - glucanase (by homology) Candida albicans
5.    2690.1                            Score: 14     Queries matched: 1
 CONTIG=Contig10227 POSCDS1=145442 POSCDS2=148636
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   551.81   1101.61   1101.57   0.04 0  14  3.5 1  K.LDALESAVER.A

 
      Proteins matching the same set of peptides:
      21813.1                           Score: 14     Queries matched: 1
6.    4929.1                            Mass: 72369    Score: 14     Queries matched: 1
 CONTIG=Contig2518 POSCDS1=198867 POSCDS2=200780
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   551.81   1101.61   1101.59   0.02 0  14  3.5 1  R.DAIELLDVSK.G

7.    929.2                             Score: 9      Queries matched: 1
 CONTIG=Contig2518 POSCDS1=57770 POSCDS2=59320
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   814.93   1627.84   1628.86   -1.02 1  9  5.6 2  K.FSKMVELVSFAPFK.G

8.    21309.1                           Mass: 15341    Score: 7      Queries matched: 1
 CONTIG=Contig20236 POSCDS1=302514 POSCDS2=302921
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
23   758.78   2273.33   2274.23   -0.91 1  7  5.4 1  R.KKPPFFFTSSFLACILMIK.L

 
      Proteins matching the same set of peptides:
      27878.1                           Mass: 15341    Score: 7      Queries matched: 1
 CONTIG=Contig10236 POSCDS1=302531 POSCDS2=302938
9.    19635.2                           Mass: 77562    Score: 6      Queries matched: 1
 CONTIG=Contig10183 POSCDS1=70303 POSCDS2=72273
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
12   626.03   1875.08   1874.88   0.20 0  6  10 1  K.FYNMQEVLFTYHQR.S

 
      Proteins matching the same set of peptides:
      19636.2                           Mass: 77695    Score: 6      Queries matched: 1
 CONTIG=Contig20183 POSCDS1=70426 POSCDS2=72399
10.   TRBOTR                            Score: 6      Queries matched: 1
 trypsin (EC3.4.21.4 ) precursor-bovine
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 23   758.78   2273.33   2272.15   1.17 0  6  7.6 2  K.SIVHPSYNSNTLNNDIMLIK.L

11.   10288.2                           Score: 6      Queries matched: 1
 CONTIG=Contig10234 POSCDS1=45773 POSCDS2=49684
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 12   626.03   1875.08   1874.99   0.09 1  6  11 2  K.KLGHLYYQVLEIDER.V

 
      Proteins matching the same set of peptides:
      21434.1                           Score: 6      Queries matched: 1
12.   5345.3                            Mass: 91902    Score: 4      Queries matched: 1
 CONTIG=Contig10163 POSCDS1=141867 POSCDS2=144284
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   717.44   1432.87   1433.81   -0.94 1  4  20 1  R.LHTSQKLNALPGR.E

 
      Proteins matching the same set of peptides:
      5612.2                            Mass: 91495    Score: 4      Queries matched: 1
 CONTIG=Contig20163 POSCDS1=141528 POSCDS2=143945
13.   26646.1                           Score: 4      Queries matched: 1
 CONTIG=Contig20064 POSCDS1=41979 POSCDS2=42434
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 12   626.03   1875.08   1874.86   0.22 0  4  15 3  R.EAICDFFAFNTSNLAR.F

 
      Proteins matching the same set of peptides:
      29745.1                           Score: 4      Queries matched: 1
14.   6747.2                            Score: 4      Queries matched: 1
 CONTIG=Contig20046 POSCDS1=144897 POSCDS2=147896
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 20   557.08   2224.30   2224.17   0.13 1  4  11 2  R.NISSVVTTFTGNESSKISVVR.F

 
      Proteins matching the same set of peptides:
      29919.1                           Score: 4      Queries matched: 1
15.   6455.2                            Mass: 90063    Score: 4      Queries matched: 1
 CONTIG=Contig10135 POSCDS1=50084 POSCDS2=52453
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
22   1137.16   2272.31   2271.23   1.08 1  6  8.2 1  K.QPVFQVATIDDNLKFLAGAPK.E

 
      Proteins matching the same set of peptides:
      25421.1                           Mass: 90922    Score: 4      Queries matched: 1
 CONTIG=Contig20135 POSCDS1=50067 POSCDS2=52457
16.   7198.1                            Score: 3      Queries matched: 1
 CONTIG=Contig20150 POSCDS1=93496 POSCDS2=94443
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 8   790.97   1579.93   1580.82   -0.89 0  3  23 2  R.IHQLEQQQLETSK.A

 
      Proteins matching the same set of peptides:
      16941.2                           Score: 3      Queries matched: 1
17.   sw|P35527|K1CI_HUMAN              Mass: 62178    Score: 3      Queries matched: 1
 Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   618.85   1235.69   1234.52   1.17 0  3  35 1  R.FSSSSGYGGGSSR.V

18.   8573.1                            Score: 3      Queries matched: 1
 CONTIG=Contig20231 POSCDS1=65129 POSCDS2=66940
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   717.44   1432.87   1431.70   1.16 0  3  30 2  R.MITSCGHIICLK.C

 
      Proteins matching the same set of peptides:
      27959.1                           Score: 3      Queries matched: 1
19.   1063.2                            Score: 2      Queries matched: 1
 CONTIG=Contig10227 POSCDS1=40794 POSCDS2=44453
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 15   970.10   1938.19   1938.02   0.16 1  2  19 2  K.ELSSPLEAHSKQVLCLK.I

 
      Proteins matching the same set of peptides:
      21794.1                           Score: 2      Queries matched: 1
20.   12120.1                           Score: 2      Queries matched: 1
 CONTIG=Contig10231 POSCDS1=20148 POSCDS2=21230
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 22   1137.16   2272.31   2271.13   1.18 0  2  18 2  R.INLAINYDLPNEADQYLHR.V

 
      Proteins matching the same set of peptides:
      18224.2                           Score: 2      Queries matched: 1
Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
20   557.08   2224.30   2225.22   -0.92 1  5  9.7 1  VLKNLLQDIILYSNNYFR
17   1082.11   2162.21   2162.27   -0.06 1  2  22 1  FFSYPVLVVLVLKILPCR
5   635.90   1269.79   1268.70   1.08 0  2  39 1  GAHLSFFLLHK
11   855.46   1708.91   1707.86   1.05 0  2  34 1  YTEEITDLNIQIEK
1   497.79   993.57        
2   540.82   1079.62        
7   787.49   1572.96        
13   968.52   1935.02        
16   664.05   1989.14        
21   756.78   2267.31        
25   850.82   2549.43        
26   1039.35   3115.04        
27   1178.22   3531.62        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 27

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 2
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 3
Score greater than 26 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 13.9    0.02    6  4929.1 R.DAIELLDVSK.G
 13.9    0.04    5  2690.1 K.LDALESAVER.A
Top scoring peptide matches to query 4
Score greater than 15 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein              Peptide
  2.9    1.17   17  sw|P35527|K1CI_HUMAN R.FSSSSGYGGGSSR.V
Top scoring peptide matches to query 5
Score greater than 14 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.9    1.08        K.GAHLSFFLLHK.K
Top scoring peptide matches to query 6
Score greater than 15 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  4.5   -0.94   12  5345.3 R.LHTSQKLNALPGR.E
  2.6    1.16   18  8573.1 R.MITSCGHIICLK.C
  1.6   -0.76              R.QLENSEDSSSPNK.E
Top scoring peptide matches to query 7
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 8
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 24.0    0.14    4  15898.1 R.LYGVDCSQVEAVLK.A
  3.3   -0.89   16  7198.1  R.IHQLEQQQLETSK.A
Top scoring peptide matches to query 9
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 26.8    0.14    3  mUTR2 R.IQFSLWPGGDSSNAK.G
Top scoring peptide matches to query 10
Score greater than 24 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 38.8    0.14    2  mSSR1 K.CSGLNDLSCICTTK.N
  9.5   -1.02    7  929.2 K.FSKMVELVSFAPFK.G
Top scoring peptide matches to query 11
Score greater than 14 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.6    1.05        K.YTEEITDLNIQIEK.K
Top scoring peptide matches to query 12
Score greater than 18 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  6.0    0.20    9  19635.2 K.FYNMQEVLFTYHQR.S
  5.5    0.09   11  10288.2 K.KLGHLYYQVLEIDER.V
  4.4    0.22   13  26646.1 R.EAICDFFAFNTSNLAR.F
  1.6    0.14               K.GFGKRPANDASWSDIVR.E
  1.5    1.11               K.DLGIASELWDPSFIRR.W
  1.1    1.19               K.TRSDNSIFFEVDGPYK.A
  1.1    0.25               K.MFYCGLIFGFFSCAR.H
  1.0   -0.87               R.AKQNIMDLAIFDNIDR.K
  0.3    0.15               R.FSQGFKELLHDVNDAR.R
  0.1    1.17               R.MTVDEVDQEAIQEVLR.V
Top scoring peptide matches to query 13
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 14
Score greater than 19 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  6.4    0.16    1  mCHT2 K.LFVGVPATSNIAGYVDTSK.L
Top scoring peptide matches to query 15
Score greater than 16 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 13.4    0.18    1  mCHT2  K.LFVGVPATSNIAGYVDTSK.L
  2.5    0.16   19  1063.2 K.ELSSPLEAHSKQVLCLK.I
  0.9   -0.76              K.ANGSSLKVAFESVEMTNR.S
  0.7   -0.75              R.AVTFPYDDPTIPAETFR.V
  0.3    0.28              K.AYSDSPMTIQDLEAEIR.A
  0.2   -0.75              R.ISCIEGLIDPYFDELR.N
  0.1    0.30              -.PSQLYMNNNVASSQNSGK.S
Top scoring peptide matches to query 16
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 17
Score greater than 14 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.9   -0.06        R.FFSYPVLVVLVLKILPCR.A
Top scoring peptide matches to query 18
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 22.1    0.19    1  mCHT2 K.TVLLSLGGGVGDYGFSDVASATK.F
Top scoring peptide matches to query 19
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 45.0    1.14    1  mCHT2 K.TVLLSLGGGVGDYGFSDVASATK.F
Top scoring peptide matches to query 20
Score greater than 17 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  4.6   -0.92              K.VLKNLLQXIILYSNNYFR.T
  4.0    0.13   14  6747.2 R.NISSVVTTFTGNESSKISVVR.F
Top scoring peptide matches to query 21
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 22
Score greater than 15 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  5.9    1.08   15  6455.2  K.QPVFQVATIDDNLKFLAGAPK.E
  2.4    1.18   20  12120.1 R.INLAINYDLPNEADQYLHR.V
Top scoring peptide matches to query 23
Score greater than 18 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  7.2   -0.91    8  21309.1 R.KKPPFFFTSSFLACILMIK.L
  5.7    1.17   10  TRBOTR  K.SIVHPSYNSNTLNNDIMLIK.L
Top scoring peptide matches to query 24
Score greater than 18 indicates homology
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  5.3    0.21    1  mCHT2 K.NYFLSAAPQCPYPDASLGDLLSK.V
Top scoring peptide matches to query 25
Score greater than 27 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 26
Score greater than 24 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 27
Score greater than 24 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide