Mascot Search Results

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MS data file    : cw0238_q5.pkl
Database        : CAlbicans dos (20286 sequences; 6720281 residues)
Timestamp       : 6 Sep 2005 at 08:38:12 GMT
Significant hits: mALS1.5eoc  |predicted| C. albicans |CA0909| 658 aa - agglutinin-like,5-prime end
                  mALS3.5eoc  |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end
                  mSod5/Pga3  |validated by us| mIPF1222 C. albicans |CA4836| 228 aa

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 31 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    mALS1.5eoc                        Mass: 69052    Score: 142    Queries matched: 2
 |predicted| C. albicans |CA0909| 658 aa - agglutinin-like,5-prime end
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   532.38   1062.75   1062.58   0.17 0  49  0.00076 1  R.SQSKPFTLR.W
21   843.53   1685.05   1684.82   0.23 0  93  2.7e-008 1  K.YTTSQTSVDLTADGVK.Y

 
      Proteins matching the same set of peptides:
      13002.2                           Mass: 133694   Score: 142    Queries matched: 2
 |Als1| CONTIG=Contig10233 POSCDS1=72223 POSCDS2=76005
      21498.1                           Mass: 133402   Score: 142    Queries matched: 2
 |Als1| CONTIG=Contig20233 POSCDS1=71475 POSCDS2=75257

2.    mALS3.5eoc                        Mass: 94266    Score: 77     Queries matched: 3
 |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   352.74   703.46   703.40   0.06 0  15  4.1 1  R.APFTLR.W
22   846.54   1691.07   1690.85   0.21 0  56  0.00011 1  K.FTTSQTSVDLTAHGVK.Y
23   909.54   1817.07   1816.84   0.23 1  6  13 1  K.CFTAGTNTVTFNDGGKK.I

 
      Proteins matching the same set of peptides:
      12561.3                           Mass: 95687    Score: 77     Queries matched: 3
 |Als3.5'| CONTIG=Contig10129 POSCDS1=15287 POSCDS2=17932
      25511.1                           Mass: 95687    Score: 77     Queries matched: 3
 |Als3.5'| CONTIG=Contig20129 POSCDS1=15283 POSCDS2=17928
3.    mSod5/Pga3                        Mass: 22006    Score: 76     Queries matched: 1
 |validated by us| mIPF1222 C. albicans |CA4836| 228 aa
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
15   704.46   1406.90   1406.72   0.18 0  76  1.5e-006 1  R.AATPAAHEVGDLAGK.H

 
      Proteins matching the same set of peptides:
      1222.2                            Mass: 23689    Score: 76     Queries matched: 1
 |Pga3/Sod5| CONTIG=Contig10139 POSCDS1=124618 POSCDS2=125304
      25296.1                           Mass: 26345    Score: 76     Queries matched: 1
 CONTIG=Contig20139 POSCDS1=124373 POSCDS2=125059
4.    22365.1                           Score: 12     Queries matched: 1
 CONTIG=Contig20215 POSCDS1=355726 POSCDS2=355977
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   352.74   703.46   703.37   0.10 0  12  8.8 2  R.APFDVR.G

 
      Proteins matching the same set of peptides:
      28240.1                           Score: 12     Queries matched: 1
5.    mRBT5                             Mass: 22745    Score: 9      Queries matched: 1
 |predicted| C. albicans fungus|CA2558| 241 aa
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
12   618.88   1235.75   1235.59   0.16 0  9  7.6 1  R.IYDQLPECAK.E

 
      Proteins matching the same set of peptides:
      mPga10                            Mass: 23610    Score: 9      Queries matched: 1
 |predicted| C. albicans fungus|CA4381|mIPF20169: 250 aa
      19952.1                           Mass: 24614    Score: 9      Queries matched: 1
 |Rbt5| CONTIG=Contig10231 POSCDS1=6478 POSCDS2=7203
      20169.1                           Mass: 25603    Score: 9      Queries matched: 1
 |Pga10| CONTIG=Contig10231 POSCDS1=59560 POSCDS2=60312
      21543.1                           Mass: 25731    Score: 9      Queries matched: 1
 CONTIG=Contig20231 POSCDS1=59564 POSCDS2=60316
      21544.1                           Mass: 25457    Score: 9      Queries matched: 1
 CONTIG=Contig20231 POSCDS1=6478 POSCDS2=7203
6.    28817.1                           Score: 8      Queries matched: 1
 CONTIG=Contig10173 POSCDS1=102887 POSCDS2=103240
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   532.38   1062.75   1061.56   1.20 1  8  9.8 2  K.ITSTRNNTR.K

7.    1035.1                            Score: 7      Queries matched: 1
 CONTIG=Contig10212 POSCDS1=14225 POSCDS2=14692
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   532.38   1062.75   1062.53   0.22 0  7  12 3  R.QSSSTLTPSR.I

 
      Proteins matching the same set of peptides:
      22653.1                           Score: 7      Queries matched: 1
8.    13836.2                           Mass: 37190    Score: 7      Queries matched: 1
 CONTIG=Contig10146 POSCDS1=76347 POSCDS2=77504
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   425.25   848.49   847.44   1.05 1  7  20 1  K.AGKTADSAK.D

 
      Proteins matching the same set of peptides:
      24991.1                           Mass: 37577    Score: 7      Queries matched: 1
 CONTIG=Contig20146 POSCDS1=76357 POSCDS2=77520
9.    1719.2                            Score: 6      Queries matched: 1
 CONTIG=Contig10166 POSCDS1=12672 POSCDS2=13763
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   352.74   703.46   703.40   0.06 0  6  32 4  R.YGPVIR.L

 
      Proteins matching the same set of peptides:
      24184.1                           Score: 6      Queries matched: 1
10.   hemogl_alpha                      Mass: 15174    Score: 6      Queries matched: 1
 chain human
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
19   765.48   1528.94   1528.73   0.22 0  6  13 1  K.VGAHAGEYGAEALER.M

11.   7679.2                            Score: 6      Queries matched: 1
 CONTIG=Contig10113 POSCDS1=82341 POSCDS2=84584
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   352.74   703.46   703.36   0.10 0  6  36 5  K.GGSNTIR.S

 
      Proteins matching the same set of peptides:
      25931.1                           Score: 6      Queries matched: 1
12.   1118.1                            Mass: 23020    Score: 6      Queries matched: 1
 CONTIG=Contig10227 POSCDS1=89628 POSCDS2=90257
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
24   903.84   2708.50   2707.38   1.12 0  6  6 1  R.QQSPVLDLVHPISSGNLTTTSQETR.T

 
      Proteins matching the same set of peptides:
      21761.1                           Mass: 23107    Score: 6      Queries matched: 1
 CONTIG=Contig20227 POSCDS1=89616 POSCDS2=90182
13.   12246.2                           Score: 5      Queries matched: 1
 CONTIG=Contig10140 POSCDS1=16265 POSCDS2=17455
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   352.74   703.46   702.38   1.08 0  5  40 6  R.QWTIR.K

 
      Proteins matching the same set of peptides:
      25188.1                           Score: 5      Queries matched: 1
14.   11865.1                           Score: 5      Queries matched: 1
 CONTIG=Contig10057 POSCDS1=48147 POSCDS2=49046
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 24   903.84   2708.50   2709.33   -0.83 0  5  7.3 2  K.QIDQIYTPWSSWVVATSSDSLQAK.S

 
      Proteins matching the same set of peptides:
      26715.1                           Score: 5      Queries matched: 1
15.   16143.2                           Score: 4      Queries matched: 1
 CONTIG=Contig10143 POSCDS1=52044 POSCDS2=52859
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   352.74   703.46   702.40   1.06 0  6  31 3  M.LNSTIR.S

16.   6070.2                            Score: 4      Queries matched: 1
 CONTIG=Contig10216 POSCDS1=15263 POSCDS2=17434
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   532.38   1062.75   1062.59   0.16 1  4  24 4  R.KSITASSLEK.D

 
      Proteins matching the same set of peptides:
      13892.3                           Score: 4      Queries matched: 1
17.   22073.1                           Score: 4      Queries matched: 1
 CONTIG=Contig20219 POSCDS1=56640 POSCDS2=57251
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   352.74   703.46   703.35   0.11 0  4  52 9  K.DLSVDR.S

18.   TRBOTR                            Mass: 24662    Score: 4      Queries matched: 1
 trypsin (EC3.4.21.4 ) precursor-bovine
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   577.37   1152.73   1152.57   0.16 0  4  27 1  K.SSGTSYPDVLK.C

19.   21410.1                           Mass: 33469    Score: 3      Queries matched: 1
 CONTIG=Contig20234 POSCDS1=105333 POSCDS2=106175
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   394.76   787.50   786.50   1.00 1  3  51 1  K.TKAGILGK.Q

 
      Proteins matching the same set of peptides:
      11229.1                           Mass: 32404    Score: 2      Queries matched: 1
 CONTIG=Contig10234 POSCDS1=105342 POSCDS2=106184
20.   1872.1                            Score: 3      Queries matched: 1
 CONTIG=Contig10236 POSCDS1=75467 POSCDS2=78760
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   532.38   1062.75   1061.59   1.16 0  3  29 5  R.LTVSITCIR.D

 
      Proteins matching the same set of peptides:
      21335.1                           Score: 3      Queries matched: 1
Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
10   596.37   1190.73   1189.62   1.11 1  3  39 1  RVNDYPTAVR
14   689.95   1377.88   1377.61   0.27 1  3  33 1  QTNDNNRQCTK
11   606.92   1211.83   1210.68   1.15 1  2  32 1  KTSSLYLIISS
2   385.78   769.54   768.42   1.13 1  2  48 1  FPYSKK
13   645.39   1288.77   1289.69   -0.92 1  1  51 1  NMSLNPALKFR
5   441.84   881.66   882.38   -0.72 0  1  47 1  FLLCDSE
16   716.95   1431.88   1431.77   0.11 1  1  49 1  KLYNETAAQGLPK
6   501.84   1001.67        
8   542.35   1082.69        
17   742.46   1482.91        
18   747.40   1492.78        
20   797.98   1593.94        
25   919.21   2754.59        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 25

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 25 indicates homology
Score greater than 34 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 15.1    0.06    2  mALS3.5eoc R.APFTLR.W
 11.8    0.10    4  22365.1    R.APFDVR.G
  6.4    1.06   15  16143.2    M.LNSTIR.S
  6.2    0.06    9  1719.2     R.YGPVIR.L
  5.6    0.10   11  7679.2     K.GGSNTIR.S
  5.3    1.08   13  12246.2    R.QWTIR.K
  5.3    0.10                  R.EWTIR.K
  5.3    0.04                  R.TITTIR.T
  4.1    1.06                  R.NISITR.A
  4.1    0.11   17  22073.1    K.DLSVDR.S
Top scoring peptide matches to query 2
Score greater than 14 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.6    1.13        R.FPYSKK.F
Top scoring peptide matches to query 3
Score greater than 16 indicates homology
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  3.5    1.00   19  21410.1 K.TKAGILGK.Q
Top scoring peptide matches to query 4
Score greater than 14 indicates homology
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  7.1    1.05    8  13836.2 K.AGKTADSAK.D
  1.4    1.12               R.QTDNDQK.T
  1.0    0.97               K.FQVIKLT.-
  0.5    1.04               R.NAGPFKSK.L
  0.2    0.01               -.KQTITMK.K
Top scoring peptide matches to query 5
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  0.8   -0.72        R.FLLCDSE.-
Top scoring peptide matches to query 6
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 7
Score greater than 24 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 49.1    0.17    1  mALS1.5eoc R.SQSKPFTLR.W
  8.0    1.20    6  28817.1    K.ITSTRNNTR.K
  7.3    0.22    7  1035.1     R.QSSSTLTPSR.I
  4.2    0.16   16  6070.2     R.KSITASSLEK.D
  3.3    1.16   20  1872.1     R.LTVSITCIR.D
  2.8    1.17                  K.LNDLAFTLR.N
  0.8    0.26                  K.SNQTELDTR.L
  0.2    0.20                  R.SSNCLLTIR.S
Top scoring peptide matches to query 8
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 9
Score greater than 15 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  4.0    0.16   18  TRBOTR K.SSGTSYPDVLK.C
  2.2    1.11              K.YIHSAGVIHR.D
  1.4   -0.92              K.QETAHIISKK.L
  1.4    1.16              K.KGFHTNFSSK.S
Top scoring peptide matches to query 10
Score greater than 13 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.7    1.11        R.RVNDYPTAVR.T
  0.3    0.12        R.EKLCIIMER.I
Top scoring peptide matches to query 11
Score greater than 14 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.3    1.15        R.KTSSLYLIISS.-
  0.9    0.16        K.QICHITSLLK.D
Top scoring peptide matches to query 12
Score greater than 22 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  9.4    0.16    5  mRBT5 IYDQLPECAK
Top scoring peptide matches to query 13
Score greater than 13 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.0   -0.92        K.NMSLNPALKFR.D
Top scoring peptide matches to query 14
Score greater than 15 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.6    0.27        K.QTNDNNRQCTK.Y
  2.6    0.12        K.EQLTPAGPISPLR.L
  2.2   -0.85        R.QVGVIFTEAYPR.K
  2.0   -0.72        K.QDDKTSCPLCR.K
Top scoring peptide matches to query 15
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 75.7    0.18    3  mSod5/Pga3 R.AATPAAHEVGDLAGK.H
Top scoring peptide matches to query 16
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  0.6    0.11        R.KLYNETAAQGLPK.V
Top scoring peptide matches to query 17
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 18
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 19
Score greater than 15 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
  5.9    0.22   10  hemogl_alpha K.VGAHAGEYGAEALER.M
  2.5    1.20                    R.EWEIPEVIVDDGK.V
  0.9    0.06                    K.KPVFISKNWVVGR.S
Top scoring peptide matches to query 20
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 21
Score greater than 20 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 92.5    0.23    1  mALS1.5eoc K.YTTSQTSVDLTADGVK.Y
  1.4    0.11                  -.LLVGSIGVDTLEADKR.V
Top scoring peptide matches to query 22
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 56.0    0.21    2  mALS3.5eoc K.FTTSQTSVDLTAHGVK.Y
Top scoring peptide matches to query 23
Score greater than 18 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
  5.6    0.23    2  mALS3.5eoc K.CFTAGTNTVTFNDGGKK.I
Top scoring peptide matches to query 24
Score greater than 18 indicates homology
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  5.6    1.12   12  1118.1  R.QQSPVLDLVHPISSGNLTTTSQETR.T
  4.8   -0.83   14  11865.1 K.QIDQIYTPWSSWVVATSSDSLQAK.S
  3.0    1.02               K.QDLQNQPTLLADGDYLVIAGLIPIK.F
  2.8    1.00               K.AGATPEVQVVDEVLKALEGLTISQLK.E
  2.5    0.04               K.KPELYLKVGIAAPAGVLMWGPPGCGK.T
  2.5    1.13               K.QLIANKSGLISTTTLPDYESAGWDK.I
  2.0   -0.85               R.KIDLYLLPVMCLLYCFQFMDK.L
  2.0   -0.92               R.QIDWANLEILSSQEINELLLGSPK.E
  1.8    0.02               K.QGVIGEGTHFLVPWLQKAVIFDVR.V
  1.6   -0.94               K.SAVAKQTSLAAVQSSAASVQLSAAHAQK.S
Top scoring peptide matches to query 25
Score greater than 25 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide