Mascot Search Results

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MS data file    : cw0237_q2.pkl
Database        : CAlbicans dos (20286 sequences; 6720281 residues)
Timestamp       : 11 Aug 2005 at 11:25:17 GMT
Significant hits: TRBOTR      trypsin (EC3.4.21.4 ) precursor-bovine
                  mALS1.5eoc  |predicted| C. albicans |CA0909| 658 aa - agglutinin-like,5-prime end
                  mALS3.5eoc  |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 30 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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  Significance threshold p< Max. number of hits  
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1.    TRBOTR                            Mass: 24662    Score: 67     Queries matched: 2
 trypsin (EC3.4.21.4 ) precursor-bovine
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   577.34   1152.67   1152.57   0.11 0  12  4.8 1  K.SSGTSYPDVLK.C
17   758.44   2272.30   2272.15   0.15 0  55  9.4e-005 1  K.SIVHPSYNSNTLNNDIMLIK.L

2.    mALS1.5eoc                        Mass: 69052    Score: 65     Queries matched: 2
 |predicted| C. albicans |CA0909| 658 aa - agglutinin-like,5-prime end
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
10   708.41   1414.81   1414.67   0.13 0  59  7.9e-005 1  K.STVDPSGYLYASR.V
13   843.48   1684.94   1684.82   0.12 0  6  13 1  K.YTTSQTSVDLTADGVK.Y

 
      Proteins matching the same set of peptides:
      13002.2                           Mass: 133694   Score: 65     Queries matched: 2
 |Als1| CONTIG=Contig10233 POSCDS1=72223 POSCDS2=76005
      21498.1                           Mass: 133402   Score: 65     Queries matched: 2
 |Als1| CONTIG=Contig20233 POSCDS1=71475 POSCDS2=75257
3.    mALS3.5eoc                        Mass: 94266    Score: 45     Queries matched: 2
 |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   546.83   1091.64   1091.56   0.08 0  45  0.0027 1  K.ISINVDFER.S
 9   696.39   1390.77   1389.66   1.11 0  0  64 2  K.TCSSNGIFITYK.N

 
      Proteins matching the same set of peptides:
      12561.3                           Mass: 95687    Score: 45     Queries matched: 2
 |Als3.5'| CONTIG=Contig10129 POSCDS1=15287 POSCDS2=17932
      25511.1                           Mass: 95687    Score: 45     Queries matched: 2
 |Als3.5'| CONTIG=Contig20129 POSCDS1=15283 POSCDS2=17928
4.    mEcm33.3                          Mass: 41984    Score: 29     Queries matched: 1
 |predicted|m5366.2|CaECM33
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
12   787.47   1572.93   1572.81   0.12 0  29  0.06 1  K.TIGGALQISDNSELR.S

 
      Proteins matching the same set of peptides:
      5366.2                            Mass: 43798    Score: 29     Queries matched: 1
 |Ecm33.3| CaECM33 CONTIG=Contig10163 POSCDS1=164904 POSCDS2=166142
      24311.1                           Mass: 42880    Score: 29     Queries matched: 1
 CONTIG=Contig20163 POSCDS1=164513 POSCDS2=165751
5.    mCHT2                             Mass: 59565    Score: 26     Queries matched: 1
 583 aa - chitinase 2 precursor Candida albicans
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
14   968.51   1935.01   1933.90   1.11 0  26  0.12 1  -.SNQVALYWGQNGAGGQER.L

6.    mIPF8527|mPga29:                  Mass: 19928    Score: 14     Queries matched: 1
 189 aa - Candida albicans unknown function
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   554.36   1106.71   1106.63   0.08 0  14  2.7 1  -.TISSIQLFAK.S

7.    hemogl_alpha                      Mass: 15174    Score: 14     Queries matched: 1
 chain human
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   510.62   1528.83   1528.73   0.10 0  14  2.2 1  K.VGAHAGEYGAEALER.M

8.    44.1                              Score: 12     Queries matched: 1
 CONTIG=Contig10236 POSCDS1=217539 POSCDS2=218771
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   577.34   1152.67   1151.53   1.14 1  12  4.9 2  K.NGKNNNYSNK.H

 
      Proteins matching the same set of peptides:
      21226.1                           Score: 12     Queries matched: 1
9.    12606.1                           Score: 9      Queries matched: 1
 CONTIG=Contig10173 POSCDS1=3 POSCDS2=2555
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   546.83   1091.64   1091.63   0.01 0  9  9.9 2  K.LSILSGFISR.L

 
      Proteins matching the same set of peptides:
      23911.1                           Score: 9      Queries matched: 1
10.   3047.1                            Mass: 6497     Score: 9      Queries matched: 1
 CONTIG=Contig10216 POSCDS1=239740 POSCDS2=239916
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
15   721.74   2162.19   2162.08   0.12 0  9  4.2 1  K.INSNAPTPPFPNIAFNTYGK.T

 
      Proteins matching the same set of peptides:
      22241.1                           Mass: 6497     Score: 9      Queries matched: 1
 CONTIG=Contig20216 POSCDS1=240178 POSCDS2=240354
11.   24924.1                           Score: 9      Queries matched: 1
 CONTIG=Contig20148 POSCDS1=46786 POSCDS2=47613
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   708.41   1414.81   1414.64   0.17 1  9  8 2  -.STTMQDSFDQKK.Q

12.   mRBT5                             Mass: 22745    Score: 8      Queries matched: 1
 |predicted| C. albicans fungus|CA2558| 241 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   618.85   1235.68   1235.59   0.10 0  8  10 1  R.IYDQLPECAK.E

 
      Proteins matching the same set of peptides:
      mPga10                            Mass: 23610    Score: 8      Queries matched: 1
 |predicted| C. albicans fungus|CA4381|mIPF20169: 250 aa
      19952.1                           Mass: 24614    Score: 8      Queries matched: 1
 |Rbt5| CONTIG=Contig10231 POSCDS1=6478 POSCDS2=7203
      20169.1                           Mass: 25603    Score: 8      Queries matched: 1
 |Pga10| CONTIG=Contig10231 POSCDS1=59560 POSCDS2=60312
      21543.1                           Mass: 25731    Score: 8      Queries matched: 1
 CONTIG=Contig20231 POSCDS1=59564 POSCDS2=60316
      21544.1                           Mass: 25457    Score: 8      Queries matched: 1
 CONTIG=Contig20231 POSCDS1=6478 POSCDS2=7203
13.   8830.2                            Score: 8      Queries matched: 1
 CONTIG=Contig10051 POSCDS1=26847 POSCDS2=28985
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 17   758.44   2272.30   2273.29   -0.99 1  8  5 2  K.QILHALAINANNESVGQKLLK.N

 
      Proteins matching the same set of peptides:
      26907.1                           Score: 8      Queries matched: 1
14.   77.2                              Score: 8      Queries matched: 1
 CONTIG=Contig10236 POSCDS1=177843 POSCDS2=178322
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   546.83   1091.64   1091.60   0.04 0  8  14 3  K.ISLETLFNR.W

 
      Proteins matching the same set of peptides:
      21281.1                           Score: 8      Queries matched: 1
15.   4009.2                            Score: 7      Queries matched: 1
 CONTIG=Contig10150 POSCDS1=137214 POSCDS2=140846
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   546.83   1091.64   1091.50   0.14 1  7  15 6  K.IAENSKCDR.K

 
      Proteins matching the same set of peptides:
      24887.1                           Score: 7      Queries matched: 1
16.   4131.2                            Score: 7      Queries matched: 1
 CONTIG=Contig10139 POSCDS1=90516 POSCDS2=91856
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 10   708.41   1414.81   1414.80   0.01 0  7  13 3  R.IAMLLFNIPDIR.L

 
      Proteins matching the same set of peptides:
      25267.1                           Score: 7      Queries matched: 1
17.   14199.2                           Mass: 69267    Score: 6      Queries matched: 1
 CONTIG=Contig20090 POSCDS1=5074 POSCDS2=6942
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
16   734.39   2200.15   2199.16   0.99 1  6  8.8 1  K.LLNMDLQFHLSRQTGGLTR.A

 
      Proteins matching the same set of peptides:
      19157.2                           Mass: 84194    Score: 6      Queries matched: 1
 CONTIG=Contig10090 POSCDS1=4693 POSCDS2=6945
18.   621.1                             Score: 6      Queries matched: 1
 CONTIG=Contig2518 POSCDS1=157092 POSCDS2=158630
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   546.83   1091.64   1092.58   -0.94 0  8  14 3  R.NVTPIGYTTK.E

19.   6439.3                            Score: 6      Queries matched: 1
 CONTIG=Contig20183 POSCDS1=37172 POSCDS2=40570
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   546.83   1091.64   1091.62   0.02 0  8  14 3  R.ISIGSIDLFK.L

 
      Proteins matching the same set of peptides:
      28743.1                           Score: 6      Queries matched: 1
20.   11881.2                           Mass: 102010   Score: 5      Queries matched: 2
 CONTIG=Contig10225 POSCDS1=5530 POSCDS2=8331
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   453.29   904.56   904.54   0.02 1  5  34 1  K.IKIQFEK.N
 15   721.74   2162.19   2163.03   -0.84 1  0  32 3  K.SYSSSDRPIIAYYGRSADR.S

 
      Proteins matching the same set of peptides:
      21817.1                           Mass: 106704   Score: 5      Queries matched: 2
 CONTIG=Contig20225 POSCDS1=5530 POSCDS2=8331
Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   696.39   1390.77   1389.69   1.07 1  5  23 1  MTDEERQQLLK
8   463.94   1388.81   1388.70   0.11 1  2  42 1  LQERINNYDPK
2   497.79   993.56        
3   542.32   1082.63        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 17

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 17 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  4.5    0.02   20  11881.2 K.IKIQFEK.N
  3.8    0.07               K.KETNSILT.-
  3.7    0.02               K.KLLFEQK.L
  3.6   -0.87               R.QQEKDMK.R
  3.6   -0.94               K.QIGQGLYK.E
  3.1   -0.96               K.KINTTTTK.T
  3.1   -0.97               K.QLNKLYK.I
  3.1   -0.97               K.QLNKYIK.E
  2.5    0.11               K.SDLNVTEK.K
  2.1    0.05               R.KTGLEVMK.K
Top scoring peptide matches to query 2
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 3
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 4
Score greater than 24 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 44.9    0.08    3  mALS3.5eoc K.ISINVDFER.S
  9.2    0.01    9  12606.1    K.LSILSGFISR.L
  7.7    0.02   19  6439.3     R.ISIGSIDLFK.L
  7.7    0.04   14  77.2       K.ISLETLFNR.W
  7.7   -0.94   18  621.1      R.NVTPIGYTTK.E
  7.3    0.14   15  4009.2     K.IAENSKCDR.K
  5.5   -0.98                  K.LTVLTNSAFK.D
  3.9   -0.93                  K.TGRNVYDIR.T
  0.9   -0.91                  K.ILSQDDFQK.Q
Top scoring peptide matches to query 5
Score greater than 15 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein          Peptide
 14.3    0.08    6  mIPF8527|mPga29: -.TISSIQLFAK.S
  1.8    0.18                        R.ADGEAFWRR.D
  1.6    1.17                        K.SVTNVDCALK.N
  1.5   -0.84                        K.SSSSNAKGSGVK.T
  1.4    0.05                        K.HINQLKLNK.L
  1.2    1.01                        K.HQLIKLVQK.H
  1.2    0.08                        R.YSELNKILK.T
Top scoring peptide matches to query 6
Score greater than 14 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 11.6    0.11    1  TRBOTR K.SSGTSYPDVLK.C
 11.5    1.14    8  44.1   K.NGKNNNYSNK.H
  0.9    1.13              R.GDNLIAGSYDK.R
  0.7   -0.85              K.KYSDEDIER.R
  0.1    0.14              R.QTEAGDSFSIV.-
Top scoring peptide matches to query 7
Score greater than 21 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  8.2    0.10   12  mRBT5 IYDQLPECAK
Top scoring peptide matches to query 8
Score greater than 14 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  1.8    0.11        K.LQERINNYDPK.T
  1.4    0.97        K.NIGLAGLTIYIIK.K
Top scoring peptide matches to query 9
Score greater than 13 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
  4.8    1.07                  K.MTDEERQQLLK.K
  0.3    1.11    3  mALS3.5eoc K.TCSSNGIFITYK.N
Top scoring peptide matches to query 10
Score greater than 25 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 58.9    0.13    2  mALS1.5eoc K.STVDPSGYLYASR.V
  8.8    0.17   11  24924.1    -.STTMQDSFDQKK.Q
  6.8    0.01   16  4131.2     R.IAMLLFNIPDIR.L
  0.9   -0.91                  R.TSTNPNSLSPRSR.S
Top scoring peptide matches to query 11
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein      Peptide
 13.9    0.10    7  hemogl_alpha K.VGAHAGEYGAEALER.M
  0.0    1.04                    K.LQYKSEYESLLR.D
Top scoring peptide matches to query 12
Score greater than 15 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 29.4    0.12    4  mEcm33.3 K.TIGGALQISDNSELR.S
  0.2   -0.86                K.AAQKDGAVMRPTDSK.A
Top scoring peptide matches to query 13
Score greater than 16 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
  6.0    0.12    2  mALS1.5eoc K.YTTSQTSVDLTADGVK.Y
  3.4    1.01                  -.MSISSSASTLPIIHIK.D
  0.2    1.15                  K.NANETIDIIEQCHK.S
Top scoring peptide matches to query 14
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 25.7    1.11    5  mCHT2 -.SNQVALYWGQNGAGGQER.L
Top scoring peptide matches to query 15
Score greater than 14 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  9.1    0.12   10  3047.1  K.INSNAPTPPFPNIAFNTYGK.T
  1.3    0.17               K.YLKSSMPIWLMGMFDTDK.S
  0.3   -0.84   20  11881.2 K.SYSSSDRPIIAYYGRSADR.S
Top scoring peptide matches to query 16
Score greater than 18 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  6.0    0.99   17  14199.2 K.LLNMDLQFHLSRQTGGLTR.A
Top scoring peptide matches to query 17
Score greater than 24 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
 55.3    0.15    1  TRBOTR K.SIVHPSYNSNTLNNDIMLIK.L
  8.1   -0.99   13  8830.2 K.QILHALAINANNESVGQKLLK.N
  1.5   -0.88              K.KCAVGYINGDVLLYDFVSLK.L
  0.8    1.11              K.QEIFNQVVQSNPEKQGVSLK.F