Mascot Search Results

User            : 
Email           : 
Search title    : 
MS data file    : cw0194_q2.pkl
Database        : CAlbicans dos (20286 sequences; 6720281 residues)
Timestamp       : 6 Sep 2005 at 10:08:15 GMT
Significant hits: mEcm33.3    |predicted|m5366.2|CaECM33
                  mALS1.5eoc  |predicted| C. albicans |CA0909| 658 aa - agglutinin-like,5-prime end
                  mALS3.5eoc  |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 29 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

  Help
  Significance threshold p< Max. number of hits  
  Standard scoring  MudPIT scoring  Ions score cut-off Show sub-sets
  Show pop-ups  Suppress pop-ups  Sort unassigned Require bold red

        Error tolerant   

1.    mEcm33.3                          Mass: 41984    Score: 53     Queries matched: 1
 |predicted|m5366.2|CaECM33
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   787.56   1573.11   1572.81   0.30 0  53  0.00019 1  K.TIGGALQISDNSELR.S

 
      Proteins matching the same set of peptides:
      5366.2                            Mass: 43798    Score: 53     Queries matched: 1
 |Ecm33.3| CaECM33 CONTIG=Contig10163 POSCDS1=164904 POSCDS2=166142
      24311.1                           Mass: 42880    Score: 53     Queries matched: 1
 CONTIG=Contig20163 POSCDS1=164513 POSCDS2=165751

2.    mALS1.5eoc                        Mass: 69052    Score: 40     Queries matched: 1
 |predicted| C. albicans |CA0909| 658 aa - agglutinin-like,5-prime end
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   708.47   1414.93   1414.67   0.26 0  40  0.005 1  K.STVDPSGYLYASR.V

 
      Proteins matching the same set of peptides:
      13002.2                           Mass: 133694   Score: 40     Queries matched: 1
 |Als1| CONTIG=Contig10233 POSCDS1=72223 POSCDS2=76005
      21498.1                           Mass: 133402   Score: 40     Queries matched: 1
 |Als1| CONTIG=Contig20233 POSCDS1=71475 POSCDS2=75257
3.    mALS3.5eoc                        Mass: 94266    Score: 37     Queries matched: 1
 |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   610.94   1219.87   1219.66   0.21 1  37  0.01 1  K.KISINVDFER.S

 
      Proteins matching the same set of peptides:
      12561.3                           Mass: 95687    Score: 37     Queries matched: 1
 |Als3.5'| CONTIG=Contig10129 POSCDS1=15287 POSCDS2=17932
      25511.1                           Mass: 95687    Score: 37     Queries matched: 1
 |Als3.5'| CONTIG=Contig20129 POSCDS1=15283 POSCDS2=17928
4.    mSod5/Pga3                        Mass: 22006    Score: 21     Queries matched: 1
 |validated by us| mIPF1222 C. albicans |CA4836| 228 aa
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   791.55   2371.63   2371.23   0.39 0  21  0.17 1  K.GLPSDIGPFPYHVHEKPVPASK.N

 
      Proteins matching the same set of peptides:
      1222.2                            Mass: 23689    Score: 21     Queries matched: 1
 |Pga3/Sod5| CONTIG=Contig10139 POSCDS1=124618 POSCDS2=125304
      25296.1                           Mass: 26345    Score: 21     Queries matched: 1
 CONTIG=Contig20139 POSCDS1=124373 POSCDS2=125059
5.    714.1                             Mass: 60747    Score: 14     Queries matched: 1
 CONTIG=Contig10225 POSCDS1=137903 POSCDS2=139516
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   610.94   1219.87   1220.58   -0.71 0  14  2.2 2  K.QNSIDVNEFR.L

 
      Proteins matching the same set of peptides:
      21843.1                           Mass: 63014    Score: 14     Queries matched: 1
 CONTIG=Contig20225 POSCDS1=137901 POSCDS2=139514
6.    2817.1                            Score: 14     Queries matched: 1
 CONTIG=Contig10254 POSCDS1=112959 POSCDS2=114299
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   610.94   1219.87   1219.66   0.21 1  14  2.2 3  K.KLDEGLNLYR.V

 
      Proteins matching the same set of peptides:
      20366.1                           Score: 14     Queries matched: 1
7.    17021.2                           Score: 7      Queries matched: 1
 CONTIG=Contig10248 POSCDS1=25792 POSCDS2=27645
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   610.94   1219.87   1219.64   0.23 0  7  11 4  R.QLSVYQPMVR.F

8.    8109.2                            Score: 3      Queries matched: 1
 CONTIG=Contig20163 POSCDS1=24036 POSCDS2=24698
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   610.94   1219.87   1219.66   0.21 0  3  27 5  R.GAWGKPHGLAAR.V

 
      Proteins matching the same set of peptides:
      28907.1                           Score: 3      Queries matched: 1
9.    19723.1                           Mass: 261815   Score: 3      Queries matched: 1
 CONTIG=Contig10241 POSCDS1=130344 POSCDS2=137165
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   746.01   1490.01   1489.83   0.19 1  3  29 1  K.KLSFGGGVTLENIR.Q

 
      Proteins matching the same set of peptides:
      20927.1                           Mass: 263618   Score: 3      Queries matched: 1
 CONTIG=Contig20241 POSCDS1=130291 POSCDS2=137157
10.   11849.2                           Score: 0      Queries matched: 1
 CONTIG=Contig10206 POSCDS1=8366 POSCDS2=10057
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   746.01   1490.01   1489.74   0.28 1  0  52 2  K.NSSGSSAATPTAPSKK.S

 
      Proteins matching the same set of peptides:
      22769.1                           Score: 0      Queries matched: 1
Peptide matches not assigned to protein hits: (no details means no match)

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   618.90   1235.79        
3   681.96   1361.91        
7   895.58   1789.14        

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Number of queries      : 8

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 29 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 37.4    0.21    3  mALS3.5eoc K.KISINVDFER.S
 14.1   -0.71    5  714.1      K.QNSIDVNEFR.L
 14.1    0.21    6  2817.1     K.KLDEGLNLYR.V
  7.0    0.23    7  17021.2    R.QLSVYQPMVR.F
  3.1    0.21    8  8109.2     R.GAWGKPHGLAAR.V
Top scoring peptide matches to query 2
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 3
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 4
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 40.0    0.26    2  mALS1.5eoc K.STVDPSGYLYASR.V
Top scoring peptide matches to query 5
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
  2.6    0.19    9  19723.1 K.KLSFGGGVTLENIR.Q
  0.1    0.28   10  11849.2 K.NSSGSSAATPTAPSKK.S
Top scoring peptide matches to query 6
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein  Peptide
 53.0    0.30    1  mEcm33.3 K.TIGGALQISDNSELR.S
Top scoring peptide matches to query 7
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein Peptide
Top scoring peptide matches to query 8
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein    Peptide
 21.4    0.39    4  mSod5/Pga3 K.GLPSDIGPFPYHVHEKPVPASK.N