Mascot Search Results

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Search title    : 
MS data file    : cw0097_q2.pkl
Database        : CAlbicans dos (20286 sequences; 6720281 residues)
Timestamp       : 21 Mar 2005 at 16:02:55 GMT
Significant hits: Glu-C       |Endoproteinase- mature protein - (Staphylococcal serine proteinase) ACC P04188
                  mSSR1       |Candida albicans 212 aa - Secretory Stress Response
                  mALS3.5eoc  |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end
                  mEcm33.3    |predicted|m5366.2|CaECM33
                  9023.3      |Scw1| CONTIG=Contig10125 POSCDS1=84138 POSCDS2=85121

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 29 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

Switch to Protein Summary Report

To create a bookmark for this report, right click this link: Peptide Summary Report (../data/20050321/F002749.dat)

        Error tolerant   

1.    Glu-C                             Mass: 29006    Score: 252    Peptides matched: 4
 |Endoproteinase- mature protein - (Staphylococcal serine proteinase) ACC P04188
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   559.32   1116.63   1116.62   0.01 0  71  6.3e-006 1  LAIVKFSPNE
5   630.82   1259.63   1259.62   0.02 0  50  0.00077 1  VVKPATMSNNAE
11   759.92   1517.83   1517.81   0.02 0  103  2.7e-009 1  APTGTFIASGVVVGKD
17   498.26   1988.99   1988.97   0.02 0  28  0.063 1  ATHGDPHALKAFPSAINQD

 
      Proteins matching the same set of peptides:
      Glu-C/V8                          Mass: 36304    Score: 250    Peptides matched: 4
 |Endoproteinase precursor (Staphylococcal serine proteinase) ACC P04188

2.    mSSR1                             Mass: 20690    Score: 179    Peptides matched: 2
 |Candida albicans 212 aa - Secretory Stress Response
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   749.90   1497.79   1497.77   0.02 0  108  9.3e-010 1  APPACLLACVAKVE
12   763.85   1525.69   1525.68   0.02 1  71  4e-006 1  LSCICTTKNSDVE

3.    mALS3.5eoc                        Mass: 94266    Score: 68     Peptides matched: 3
 |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   455.57   1363.68   1363.67   0.01 0  (28) 0.11 1  RSNVDPKGYLTD
7   682.86   1363.70   1363.67   0.02 0  53  0.00028 1  RSNVDPKGYLTD
 12   763.85   1525.69   1526.75   -1.06 0  15  1.7 2  CSNIHVGITKGLND

 
      Proteins matching the same set of peptides:
      12561.3                           Mass: 95687    Score: 68     Peptides matched: 3
 |Als3.5'| CONTIG=Contig10129 POSCDS1=15287 POSCDS2=17932
      25511.1                           Mass: 95687    Score: 68     Peptides matched: 3
 |Als3.5'| CONTIG=Contig20129 POSCDS1=15283 POSCDS2=17928
4.    mEcm33.3                          Mass: 41984    Score: 61     Peptides matched: 1
 |predicted|m5366.2|CaECM33
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   574.82   1147.63   1147.61   0.03 0  61  6.8e-005 1  KTGLTAGITSAE

 
      Proteins matching the same set of peptides:
      5366.2                            Mass: 43798    Score: 61     Peptides matched: 1
 |Ecm33.3| CaECM33 CONTIG=Contig10163 POSCDS1=164904 POSCDS2=166142
      24311.1                           Mass: 42880    Score: 61     Peptides matched: 1
 CONTIG=Contig20163 POSCDS1=164513 POSCDS2=165751
5.    9023.3                            Mass: 33980    Score: 45     Peptides matched: 1
 |Scw1| CONTIG=Contig10125 POSCDS1=84138 POSCDS2=85121
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   515.76   1029.51   1029.49   0.02 0  45  0.0034 1  KYWGIYSN

 
      Proteins matching the same set of peptides:
      15898.1                           Mass: 39582    Score: 45     Peptides matched: 1
 |Scw1| CONTIG=Contig20125 POSCDS1=83941 POSCDS2=85077
      m15898.1|mSCW1:                   Mass: 35901    Score: 45     Peptides matched: 1
 346 aa - glucanase (by homology) Candida albicans
6.    12258.2                           Mass: 94881    Score: 15     Peptides matched: 1
 CONTIG=Contig10215 POSCDS1=289938 POSCDS2=292448
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   574.82   1147.63   1147.58   0.05 0  15  2.3 2  KGIKQSNSSAE

 
      Proteins matching the same set of peptides:
      16151.2                           Score: 15     Peptides matched: 1
7.    10098.1                           Score: 13     Peptides matched: 1
 CONTIG=Contig20046 POSCDS1=13608 POSCDS2=14708
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   559.32   1116.63   1116.60   0.03 1  13  3.9 2  IATKISENLE

 
      Proteins matching the same set of peptides:
      18721.2                           Score: 13     Peptides matched: 1
8.    15466.2                           Score: 9      Peptides matched: 1
 CONTIG=Contig10143 POSCDS1=50632 POSCDS2=51816
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 12   763.85   1525.69   1525.81   -0.12 1  9  6.2 3  LNWVLADALGAGVVE

 
      Proteins matching the same set of peptides:
      25059.1                           Score: 9      Peptides matched: 1
9.    14225.2                           Score: 7      Peptides matched: 1
 CONTIG=Contig20123 POSCDS1=159451 POSCDS2=160653
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   682.86   1363.70   1363.64   0.06 0  7  11 2  FVNNINNGGSITD

 
      Proteins matching the same set of peptides:
      29346.1                           Score: 7      Peptides matched: 1
10.   22514.1                           Score: 7      Peptides matched: 1
 CONTIG=Contig20215 POSCDS1=59070 POSCDS2=59435
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 12   763.85   1525.69   1526.84   -1.15 1  7  9.5 4  DFVLFSKIFAILD

 
      Proteins matching the same set of peptides:
      28236.1                           Score: 7      Peptides matched: 1
11.   2894.1                            Score: 7      Peptides matched: 1
 CONTIG=Contig2514 POSCDS1=106009 POSCDS2=109980
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 12   763.85   1525.69   1525.75   -0.06 1  7  10 5  EASPLFRSASHPVE

12.   10159.2                           Score: 7      Peptides matched: 1
 CONTIG=Contig10212 POSCDS1=116403 POSCDS2=117584
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   559.32   1116.63   1117.58   -0.95 1  7  17 3  LANGKMLKDE

 
      Proteins matching the same set of peptides:
      22598.1                           Score: 7      Peptides matched: 1
13.   8047.2                            Score: 7      Peptides matched: 1
 CONTIG=Contig10209 POSCDS1=41098 POSCDS2=41817
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   574.82   1147.63   1147.57   0.06 1  7  17 3  KITLSDTQNE

 
      Proteins matching the same set of peptides:
      22722.1                           Score: 7      Peptides matched: 1
14.   7880.2                            Score: 7      Peptides matched: 1
 CONTIG=Contig10216 POSCDS1=68642 POSCDS2=73546
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 12   763.85   1525.69   1525.86   -0.17 0  7  11 6  QKKHLLAFGGSLVE

 
      Proteins matching the same set of peptides:
      22294.1                           Score: 7      Peptides matched: 1
15.   27080.1                           Mass: 15622    Score: 7      Peptides matched: 1
 CONTIG=Contig20046 POSCDS1=46598 POSCDS2=46987
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   725.34   1448.66   1448.80   -0.14 0  7  14 1  VLATTYFIHKKE

 
      Proteins matching the same set of peptides:
      29899.1                           Mass: 16831    Score: 7      Peptides matched: 1
 CONTIG=Contig10046 POSCDS1=46465 POSCDS2=46887
16.   17991.2                           Score: 6      Peptides matched: 1
 CONTIG=Contig10248 POSCDS1=28765 POSCDS2=30078
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 11   759.92   1517.83   1517.81   0.02 1  6  13 2  KKPNIYSLEAQVE

17.   8860.1                            Score: 6      Peptides matched: 1
 CONTIG=Contig20215 POSCDS1=396386 POSCDS2=397447
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 12   763.85   1525.69   1526.71   -1.02 0  6  12 7  NNNNNLQLQNLND

 
      Proteins matching the same set of peptides:
      28257.1                           Score: 6      Peptides matched: 1
18.   11491.2                           Mass: 41365    Score: 6      Peptides matched: 1
 CONTIG=Contig10148 POSCDS1=16719 POSCDS2=17792
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   574.82   1147.63   1147.60   0.03 1  6  20 4  AQDRAFKLAE

 
      Proteins matching the same set of peptides:
      24921.1                           Mass: 42018    Score: 6      Peptides matched: 1
 CONTIG=Contig20148 POSCDS1=16727 POSCDS2=17800
19.   10309.2                           Score: 6      Peptides matched: 1
 CONTIG=Contig10143 POSCDS1=84151 POSCDS2=84945
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   515.76   1029.51   1030.57   -1.06 1  6  26 2  RILILDSSD

 
      Proteins matching the same set of peptides:
      25068.1                           Score: 6      Peptides matched: 1
20.   5897.1                            Score: 6      Peptides matched: 1
 CONTIG=Contig10259 POSCDS1=36854 POSCDS2=38902
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   630.82   1259.63   1259.60   0.03 1  7  13 2  NSNNSIDALIAE

 
      Proteins matching the same set of peptides:
      20182.1                           Score: 6      Peptides matched: 1
Peptide matches not assigned to protein hits: (no details means no match)
List sorted by Decreasing Score. Sort by Decreasing Intensity or Increasing Query # / Mr

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   563.28   1124.55   1124.56   -0.00 0  5  21 1  TLQAQAGKHGD
13   512.92   1535.73   1535.69   0.04 0  4  25 1  ASSIYMSATSRSFE
15   522.22   1563.65   1563.74   -0.09 0  3  22 1  NTTSQPNNHNPNVK
10   502.22   1503.64   1503.74   -0.10 0  1  42 1  EPKPMVFVGAFPAD
14   519.60   1555.77         
16   603.97   1808.90         
18   1138.46   2274.91         
19   1149.45   2296.89         
20   894.33   2679.96         
21   934.67   2800.99         
22   977.38   2929.13         
23   1011.79   3032.36         
24   1054.73   3161.17         
25   1065.81   3194.41         

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : V8-DE
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Data File Name         : cw0097_q2.pkl
Number of queries      : 25

Mascot:  http://www.matrixscience.com/ 

Top scoring peptide matches to query 1
Score greater than 21 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 44.6    0.02   5                            9023.3  KYWGIYSN
  5.8   -1.06  19                           10309.2  RILILDSSD
  5.2   -0.97                                        LARHEGFSD
  4.3   -0.95                                        KFGVESSYD
  2.3   -0.93                                        GIGYYWASD
  0.8   -0.95                                        SVYKASFDD
Top scoring peptide matches to query 2
Score greater than 30 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 71.1    0.01   1                             Glu-C  LAIVKFSPNE
 13.1    0.03   7                           10098.1  IATKISENLE
  6.8   -0.95  12                           10159.2  LANGKMLKDE
  5.1   -0.01                                        LRRVFQGLE
  4.0    0.09                                        ALIAGNSDGKGD
  3.8   -0.93                                        NSPAIIFIDE
  2.5    0.03                                        TLTEINGKIE
  2.5    1.01                                        KAPAIIFIDE
  2.4    0.02                                        KDSKVNGKIE
  2.2    1.01                                        KDNLAKSVIE
Top scoring peptide matches to query 3
Score greater than 13 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  5.3   -0.00                                        TLQAQAGKHGD
Top scoring peptide matches to query 4
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 60.6    0.03   4                          mEcm33.3  KTGLTAGITSAE
 15.4    0.05   6                           12258.2  KGIKQSNSSAE
  6.8    0.06  13                            8047.2  KITLSDTQNE
  6.0    0.03  18                           11491.2  AQDRAFKLAE
  5.5    1.08                                        SQTKTPGTQAE
  5.3    0.03                                        KAKAKCDKAE
  4.9    0.05                                        AFIADIAKGNE
  3.6    1.07                                        FSTFQYILE
  3.5    0.08                                        FAVELLNNND
  3.2   -0.00                                        MSKRKKELE
Top scoring peptide matches to query 5
Score greater than 23 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 49.8    0.02   1                             Glu-C  VVKPATMSNNAE
  7.5    0.03  20                            5897.1  NSNNSIDALIAE
  4.5   -1.05                                        ESVTSVLVSVLE
  3.8   -0.09                                        KSMAKLNKLVE
  2.3   -1.02                                        MNKKTLQAQAE
  0.5   -1.06                                        MLAKFKLPNAE
Top scoring peptide matches to query 6
Score greater than 16 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 27.7    0.01   3                        mALS3.5eoc  RSNVDPKGYLTD
  1.8   -1.03                                        NVKSTYAAIQRD
  1.6    0.04                                        QNSLRSQRSSSD
  0.1    1.02                                        KINESCQNKKD
  0.1    0.94                                        FSKLIPTTTNIE
Top scoring peptide matches to query 7
Score greater than 23 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 53.5    0.02   3                        mALS3.5eoc  RSNVDPKGYLTD
  7.4    0.06   9                           14225.2  FVNNINNGGSITD
  4.0    0.06                                        NWPTFEKNLSE
  4.0    0.01                                        FLRMDIKNVND
  3.0    1.04                                        KINESCQNKKD
  2.9   -0.05                                        KSIQRSSIFIAD
  0.4    0.02                                        GTERKITINSMD
  0.4    0.02                                        NGTGLTKLGFAGND
  0.3    0.05                                        QNSLRSQRSSSD
Top scoring peptide matches to query 8
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  6.5   -0.14  15                           27080.1  VLATTYFIHKKE
  4.1   -1.06                                        FIFQNKINSNPE
  1.6    0.83                                        TVFKKDGLVSLIE
  1.4   -0.05                                        NSELNNIIYQLE
  0.1    0.90                                        TIGGKSIRVTSNSE
  0.1   -1.09                                        SYHDFIKVLTLD
Top scoring peptide matches to query 9
Score greater than 19 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
108.2    0.02   2                             mSSR1  APPACLLACVAKVE
Top scoring peptide matches to query 10
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  1.2   -0.10                                        EPKPMVFVGAFPAD
Top scoring peptide matches to query 11
Score greater than 25 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
103.2    0.02   1                             Glu-C  APTGTFIASGVVVGKD
  6.4    0.02  16                           17991.2  KKPNIYSLEAQVE
  0.6    0.01                                        FIRDKILNSIGNE
Top scoring peptide matches to query 12
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 71.0    0.02   2                             mSSR1  LSCICTTKNSDVE
 14.8   -1.06   3                        mALS3.5eoc  CSNIHVGITKGLND
  9.1   -0.12   8                           15466.2  LNWVLADALGAGVVE
  7.3   -1.15  10                           22514.1  DFVLFSKIFAILD
  7.0   -0.06  11                            2894.1  EASPLFRSASHPVE
  6.7   -0.17  14                            7880.2  QKKHLLAFGGSLVE
  6.2   -1.02  17                            8860.1  NNNNNLQLQNLND
  4.5   -0.06                                        TYSVEVINATTLSE
  4.3   -0.15                                        IARFHLCAVVIVE
  3.9   -0.06                                        KNHTIESNKNNID
Top scoring peptide matches to query 13
Score greater than 13 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  3.6    0.04                                        ASSIYMSATSRSFE
Top scoring peptide matches to query 14
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
Top scoring peptide matches to query 15
Score greater than 15 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  3.3   -0.09                                        NTTSQPNNHNPNVK
  2.1   -1.16                                        TIKTSLTMPTSEKE
Top scoring peptide matches to query 16
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
Top scoring peptide matches to query 17
Score greater than 15 indicates homology
Score greater than 28 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 27.9    0.02   1                             Glu-C  ATHGDPHALKAFPSAINQD
  1.0   -0.92                                        TTSSIGTDSSTVTFCRRD
  0.7   -0.02                                        KDNWALPMIGRIISNFD
  0.3    0.90                                        AWTMAFRILANILINIE
  0.1    0.82                                        ALEKHILSKPLITGLINE
Top scoring peptide matches to query 18
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
Top scoring peptide matches to query 19
Score greater than 26 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
Top scoring peptide matches to query 20
Score greater than 24 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
Top scoring peptide matches to query 21
Score greater than 24 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
Top scoring peptide matches to query 22
Score greater than 23 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
Top scoring peptide matches to query 23
Score greater than 24 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
Top scoring peptide matches to query 24
Score greater than 23 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
Top scoring peptide matches to query 25
Score greater than 23 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide