Mascot Search Results

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MS data file    : cw0092_q2.pkl
Database        : CAlbicans dos (20286 sequences; 6720281 residues)
Timestamp       : 21 Mar 2005 at 16:00:18 GMT
Significant hits: mSSR1       |Candida albicans 212 aa - Secretory Stress Response
                  mALS3.5eoc  |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end
                  mEcm33.3    |predicted|m5366.2|CaECM33
                  mRBT5       |predicted| C. albicans fungus|CA2558| 241 aa
                  19516.2     |Hyr1| CONTIG=Contig10216 POSCDS1=152086 POSCDS2=154845

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 31 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

Switch to Protein Summary Report

To create a bookmark for this report, right click this link: Peptide Summary Report (../data/20050321/F002744.dat)

        Error tolerant   

1.    mSSR1                             Mass: 20690    Score: 204    Peptides matched: 3
 |Candida albicans 212 aa - Secretory Stress Response
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   635.84   1269.67   1269.66   0.02 0  82  3.7e-007 1  APPACLLACVAK
11   814.86   1627.70   1627.70   0.00 0  109  6.4e-010 1  CSGLNDLSCICTTK
13   854.90   1707.79   1707.78   0.01 0  13  2.2 1  EICPNGDADTAISAFK

2.    mALS3.5eoc                        Mass: 94266    Score: 130    Peptides matched: 3
 |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   546.80   1091.58   1091.56   0.02 0  45  0.0027 1  ISINVDFER
9   696.34   1390.67   1389.66   1.01 0  24  0.27 1  TCSSNGIFITYK
12   564.63   1690.88   1690.85   0.03 0  61  4.4e-005 1  FTTSQTSVDLTAHGVK

 
      Proteins matching the same set of peptides:
      12561.3                           Mass: 95687    Score: 130    Peptides matched: 3
 |Als3.5'| CONTIG=Contig10129 POSCDS1=15287 POSCDS2=17932
      25511.1                           Mass: 95687    Score: 130    Peptides matched: 3
 |Als3.5'| CONTIG=Contig20129 POSCDS1=15283 POSCDS2=17928
3.    mEcm33.3                          Mass: 41984    Score: 91     Peptides matched: 2
 |predicted|m5366.2|CaECM33
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   410.76   819.51   819.49   0.02 0  31  0.082 1  VSGGFILK
10   787.43   1572.84   1572.81   0.03 0  60  5.2e-005 1  TIGGALQISDNSELR

 
      Proteins matching the same set of peptides:
      5366.2                            Mass: 43798    Score: 91     Peptides matched: 2
 |Ecm33.3| CaECM33 CONTIG=Contig10163 POSCDS1=164904 POSCDS2=166142
      24311.1                           Mass: 42880    Score: 91     Peptides matched: 2
 CONTIG=Contig20163 POSCDS1=164513 POSCDS2=165751
4.    mRBT5                             Mass: 22745    Score: 72     Peptides matched: 2
 |predicted| C. albicans fungus|CA2558| 241 aa
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   618.82   1235.62   1235.59   0.03 0  44  0.0026 1  IYDQLPECAK
14   584.95   1751.84   1751.82   0.02 1  28  0.075 1  IYDQLPECAKECVK

 
      Proteins matching the same set of peptides:
      mPga10                            Mass: 23610    Score: 72     Peptides matched: 2
 |predicted| C. albicans fungus|CA4381|mIPF20169: 250 aa
      19952.1                           Mass: 24614    Score: 72     Peptides matched: 2
 |Rbt5| CONTIG=Contig10231 POSCDS1=6478 POSCDS2=7203
      20169.1                           Mass: 25603    Score: 72     Peptides matched: 2
 |Pga10| CONTIG=Contig10231 POSCDS1=59560 POSCDS2=60312
      21543.1                           Mass: 25731    Score: 72     Peptides matched: 2
 CONTIG=Contig20231 POSCDS1=59564 POSCDS2=60316
      21544.1                           Mass: 25457    Score: 72     Peptides matched: 2
 CONTIG=Contig20231 POSCDS1=6478 POSCDS2=7203
5.    19516.2                           Mass: 94340    Score: 43     Peptides matched: 2
 |Hyr1| CONTIG=Contig10216 POSCDS1=152086 POSCDS2=154845
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   472.76   943.50   943.48   0.02 0  12  6.6 1  STAYLYAR
4   504.79   1007.56   1007.54   0.02 0  31  0.068 1  AAALPQYFK

 
      Proteins matching the same set of peptides:
      mIPF19516                         Mass: 90303    Score: 43     Peptides matched: 2
 |mHYR1| validated by us| C. albicans fungus|CA1576| 900 aa
      19734.1                           Mass: 94169    Score: 43     Peptides matched: 2
 |Hyr1| CONTIG=Contig20216 POSCDS1=152490 POSCDS2=155303
      m19734.1                          Mass: 91992    Score: 43     Peptides matched: 2
 |mHyr1| validated by us| C. albicans fungus 917 aa
6.    mCrh11                            Mass: 44667    Score: 16     Peptides matched: 1
 |predicted| C. albicans |CA0375|: 453 aa
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   563.31   1124.60   1124.57   0.03 0  16  1.5 1  SVLVADYSSGK

 
      Proteins matching the same set of peptides:
      14360.2                           Mass: 46818    Score: 16     Peptides matched: 1
 |Crh11| CONTIG=Contig10158 POSCDS1=92919 POSCDS2=94280
      24611.1                           Mass: 47890    Score: 16     Peptides matched: 1
 CONTIG=Contig20158 POSCDS1=86016 POSCDS2=87377
7.    12297.1                           Mass: 15525    Score: 15     Peptides matched: 1
 CONTIG=Contig10166 POSCDS1=62870 POSCDS2=63313
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 14   584.95   1751.84   1750.84   1.00 1  15  1.4 2  IYDQIPKCAQECVK

 
      Proteins matching the same set of peptides:
      24214.1                           Mass: 16067    Score: 15     Peptides matched: 1
 CONTIG=Contig20166 POSCDS1=63254 POSCDS2=63697
8.    928.1                             Score: 13     Peptides matched: 1
 CONTIG=Contig2518 POSCDS1=59592 POSCDS2=62003
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   410.76   819.51   819.49   0.02 0  13  5.2 2  VSFVLQK

9.    9417.2                            Score: 12     Peptides matched: 1
 CONTIG=Contig10215 POSCDS1=124668 POSCDS2=126953
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   504.79   1007.56   1006.62   0.94 1  12  5.6 2  VILPKYFK

 
      Proteins matching the same set of peptides:
      19046.2                           Score: 12     Peptides matched: 1
10.   10638.2                           Score: 11     Peptides matched: 1
 CONTIG=Contig20215 POSCDS1=35952 POSCDS2=36452
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   410.76   819.51   819.46   0.04 1  11  7.5 3  VMKSSIR

 
      Proteins matching the same set of peptides:
      28263.1                           Score: 11     Peptides matched: 1
11.   660.1                             Score: 11     Peptides matched: 1
 CONTIG=Contig2518 POSCDS1=120781 POSCDS2=121107
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   410.76   819.51   819.39   0.11 0  11  7.9 4  VVCWEK

12.   11847.2                           Score: 10     Peptides matched: 1
 CONTIG=Contig10206 POSCDS1=956 POSCDS2=5923
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   546.80   1091.58   1092.59   -1.01 1  10  9.1 2  LSNFKSIER

 
      Proteins matching the same set of peptides:
      22779.1                           Score: 10     Peptides matched: 1
13.   12606.1                           Score: 9      Peptides matched: 1
 CONTIG=Contig10173 POSCDS1=3 POSCDS2=2555
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   546.80   1091.58   1091.63   -0.05 0  9  9.3 3  LSILSGFISR

 
      Proteins matching the same set of peptides:
      23911.1                           Score: 9      Peptides matched: 1
14.   77.2                              Score: 9      Peptides matched: 1
 CONTIG=Contig10236 POSCDS1=177843 POSCDS2=178322
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   546.80   1091.58   1091.60   -0.02 0  9  11 4  ISLETLFNR

 
      Proteins matching the same set of peptides:
      21281.1                           Score: 9      Peptides matched: 1
15.   2175.1                            Score: 9      Peptides matched: 1
 CONTIG=Contig2506 POSCDS1=68234 POSCDS2=69229
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   410.76   819.51   819.40   0.11 0  9  13 7  SASEGEIK

16.   6403.2                            Score: 9      Peptides matched: 1
 CONTIG=Contig20158 POSCDS1=107530 POSCDS2=108552
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   410.76   819.51   820.47   -0.96 0  9  13 8  SVFIDLK

 
      Proteins matching the same set of peptides:
      18718.1                           Score: 9      Peptides matched: 1
17.   23820.1                           Score: 8      Peptides matched: 1
 CONTIG=Contig20176 POSCDS1=5113 POSCDS2=5289
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   410.76   819.51   818.53   0.98 0  8  14 9  VYAILLK

 
      Proteins matching the same set of peptides:
      28761.1                           Score: 8      Peptides matched: 1
18.   4009.2                            Score: 8      Peptides matched: 1
 CONTIG=Contig10150 POSCDS1=137214 POSCDS2=140846
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   546.80   1091.58   1091.50   0.08 1  8  13 6  IAENSKCDR

 
      Proteins matching the same set of peptides:
      24887.1                           Score: 8      Peptides matched: 1
19.   23687.1                           Score: 8      Peptides matched: 1
 CONTIG=Contig20184 POSCDS1=49345 POSCDS2=49773
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 6   563.31   1124.60   1124.79   -0.19 1  8  11 2  VSLLKVLLLK

 
      Proteins matching the same set of peptides:
      28713.1                           Score: 8      Peptides matched: 1
20.   9887.3                            Score: 7      Peptides matched: 1
 CONTIG=Contig10104 POSCDS1=43800 POSCDS2=51911
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   410.76   819.51   819.38   0.13 0  10  10 5  ADPFENK

 
      Proteins matching the same set of peptides:
      26084.1                           Score: 7      Peptides matched: 1
Peptide matches not assigned to protein hits: (no details means no match)
List sorted by Decreasing Score. Sort by Decreasing Intensity or Increasing Query # / Mr

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   497.76   993.51   992.54   0.97 0  2  47 1  VIPAQYFR
15   603.60   1807.78         

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Data File Name         : cw0092_q2.pkl
Number of queries      : 15

Mascot:  http://www.matrixscience.com/ 

Top scoring peptide matches to query 1
Score greater than 27 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 30.8    0.02   3                          mEcm33.3  VSGGFILK
 12.7    0.02   8                             928.1  VSFVLQK
 11.2    0.04  10                           10638.2  VMKSSIR
 10.9    0.11  11                             660.1  VVCWEK
  9.7    0.13  20                            9887.3  ADPFENK
  9.2    0.09                                        VCSDVIK
  8.7    0.11  15                            2175.1  SASEGEIK
  8.6   -0.96  16                            6403.2  SVFIDLK
  8.4    0.98  17                           23820.1  VYAILLK
  8.3    0.12                                        CVLDSAR
Top scoring peptide matches to query 2
Score greater than 13 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 11.7    0.02   5                           19516.2  STAYLYAR
  0.0    0.96                                        GITPRIMR
Top scoring peptide matches to query 3
Score greater than 14 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  2.0    0.97                                        VIPAQYFR
  1.8    0.02                                        AIHEYYAK
  1.1    0.95                                        EHIEKLPK
Top scoring peptide matches to query 4
Score greater than 26 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 30.9    0.02   5                           19516.2  AAALPQYFK
 11.7    0.94   9                            9417.2  VILPKYFK
  0.3    0.96                                        VNLLKHQR
Top scoring peptide matches to query 5
Score greater than 25 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 44.8    0.02   2                        mALS3.5eoc  ISINVDFER
  9.5   -1.01  12                           11847.2  LSNFKSIER
  9.4   -0.05  13                           12606.1  LSILSGFISR
  8.8   -0.02  14                              77.2  ISLETLFNR
  8.5   -0.04                                        ISIGSIDLFK
  7.8    0.08  18                            4009.2  IAENSKCDR
  7.2   -0.99                                        TGRNVYDIR
  6.3   -1.02                                        SNLLSRFTR
  5.5   -1.00                                        NVTPIGYTTK
  3.5   -1.00                                        GEKLVFSADK
Top scoring peptide matches to query 6
Score greater than 21 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 16.2    0.03   6                            mCrh11  SVLVADYSSGK
  7.7   -0.19  19                           23687.1  VSLLKVLLLK
  4.5    0.04                                        WLIEENGHK
  1.3    0.02                                        WIFIGSSSTK
Top scoring peptide matches to query 7
Score greater than 20 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 44.3    0.03   4                             mRBT5  IYDQLPECAK
  5.0    0.89                                        YLTLKIVETR
  3.4   -0.02                                        IDYQSIQIEK
  1.4   -0.99                                        DYPKIEVDMK
  0.9   -0.12                                        HRLLTEIINK
Top scoring peptide matches to query 8
Score greater than 18 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 82.3    0.02   1                             mSSR1  APPACLLACVAK
  0.6   -0.04                                        KQVATQNLAAAR
Top scoring peptide matches to query 9
Score greater than 19 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 24.1    1.01   2                        mALS3.5eoc  TCSSNGIFITYK
  4.5   -0.90                                        SSSSSSSSSSSSNSK
  3.1   -0.04                                        LTIGSLSEAGNSSR
  1.8   -0.09                                        EFLQTLEIRSR
  1.0    0.03                                        NFDYFIMAQSR
  1.0    0.00                                        NFHHGRFFSSR
  0.6   -0.09                                        TRALNVVVNYDK
Top scoring peptide matches to query 10
Score greater than 19 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 60.1    0.03   3                          mEcm33.3  TIGGALQISDNSELR
  2.8    1.01                                        GVNQEILEKATAGSR
Top scoring peptide matches to query 11
Score greater than 22 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
108.7    0.00   1                             mSSR1  CSGLNDLSCICTTK
  2.2   -0.05                                        DALSPYEARMYVVD
Top scoring peptide matches to query 12
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 60.7    0.03   2                        mALS3.5eoc  FTTSQTSVDLTAHGVK
  0.5   -0.09                                        DGSILVDIVAIVGHRK
Top scoring peptide matches to query 13
Score greater than 13 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 13.3    0.01   1                             mSSR1  EICPNGDADTAISAFK
Top scoring peptide matches to query 14
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 28.0    0.02   4                             mRBT5  IYDQLPECAKECVK
 15.1    1.00   7                           12297.1  IYDQIPKCAQECVK
Top scoring peptide matches to query 15
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide