Mascot Search Results

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MS data file    : cw0078_q3.pkl
Database        : CAlbicans dos (20286 sequences; 6720281 residues)
Timestamp       : 21 Mar 2005 at 15:59:39 GMT
Significant hits: mSSR1       |Candida albicans 212 aa - Secretory Stress Response
                  mIPF19516   |mHYR1| validated by us| C. albicans fungus|CA1576| 900 aa
                  mALS3.5eoc  |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end
                  mCHT2       583 aa - chitinase 2 precursor Candida albicans
                  mRBT5       |predicted| C. albicans fungus|CA2558| 241 aa

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 32 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

Switch to Protein Summary Report

To create a bookmark for this report, right click this link: Peptide Summary Report (../data/20050321/F002743.dat)

        Error tolerant   

1.    mSSR1                             Mass: 20690    Score: 85     Peptides matched: 1
 |Candida albicans 212 aa - Secretory Stress Response
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   635.83   1269.66   1269.66   -0.00 0  85  2e-007 1  APPACLLACVAK

2.    mIPF19516                         Mass: 90303    Score: 79     Peptides matched: 3
 |mHYR1| validated by us| C. albicans fungus|CA1576| 900 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   402.24   802.46   802.45   0.01 0  28  0.18 1  LEVVTSR
3   472.75   943.49   943.48   0.01 0  12  6.9 1  STAYLYAR
5   504.78   1007.55   1007.54   0.01 0  40  0.0093 1  AAALPQYFK

 
      Proteins matching the same set of peptides:
      m19734.1                          Mass: 91992    Score: 79     Peptides matched: 3
 |mHyr1| validated by us| C. albicans fungus 917 aa
3.    mALS3.5eoc                        Mass: 94266    Score: 54     Peptides matched: 2
 |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   546.79   1091.57   1091.56   0.00 0  39  0.01 1  ISINVDFER
10   696.32   1390.63   1389.66   0.97 0  14  2.5 1  TCSSNGIFITYK

 
      Proteins matching the same set of peptides:
      12561.3                           Mass: 95687    Score: 54     Peptides matched: 2
 |Als3.5'| CONTIG=Contig10129 POSCDS1=15287 POSCDS2=17932
      25511.1                           Mass: 95687    Score: 54     Peptides matched: 2
 |Als3.5'| CONTIG=Contig20129 POSCDS1=15283 POSCDS2=17928
4.    mCHT2                             Mass: 59565    Score: 53     Peptides matched: 1
 583 aa - chitinase 2 precursor Candida albicans
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   495.28   988.55   988.54   0.01 0  53  0.00043 1  LSSAIEEIK

 
      Proteins matching the same set of peptides:
      15860.2                           Mass: 61554    Score: 53     Peptides matched: 1
 |Cht2| CONTIG=Contig10194 POSCDS1=2376 POSCDS2=4127
      19719.2                           Mass: 51350    Score: 53     Peptides matched: 1
 CONTIG=Contig20194 POSCDS1=2710 POSCDS2=4131
5.    mRBT5                             Mass: 22745    Score: 45     Peptides matched: 1
 |predicted| C. albicans fungus|CA2558| 241 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   618.80   1235.58   1235.59   -0.00 0  45  0.002 1  IYDQLPECAK

 
      Proteins matching the same set of peptides:
      mPga10                            Mass: 23610    Score: 45     Peptides matched: 1
 |predicted| C. albicans fungus|CA4381|mIPF20169: 250 aa
      19952.1                           Mass: 24614    Score: 45     Peptides matched: 1
 |Rbt5| CONTIG=Contig10231 POSCDS1=6478 POSCDS2=7203
      20169.1                           Mass: 25603    Score: 45     Peptides matched: 1
 |Pga10| CONTIG=Contig10231 POSCDS1=59560 POSCDS2=60312
      21543.1                           Mass: 25731    Score: 45     Peptides matched: 1
 CONTIG=Contig20231 POSCDS1=59564 POSCDS2=60316
      21544.1                           Mass: 25457    Score: 45     Peptides matched: 1
 CONTIG=Contig20231 POSCDS1=6478 POSCDS2=7203
6.    mEcm33.3                          Mass: 41984    Score: 28     Peptides matched: 1
 |predicted|m5366.2|CaECM33
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   410.75   819.49   819.49   0.01 0  28  0.14 1  VSGGFILK

 
      Proteins matching the same set of peptides:
      5366.2                            Mass: 43798    Score: 28     Peptides matched: 1
 |Ecm33.3| CaECM33 CONTIG=Contig10163 POSCDS1=164904 POSCDS2=166142
      24311.1                           Mass: 42880    Score: 28     Peptides matched: 1
 CONTIG=Contig20163 POSCDS1=164513 POSCDS2=165751
7.    mCrh11                            Mass: 44667    Score: 21     Peptides matched: 1
 |predicted| C. albicans |CA0375|: 453 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   563.30   1124.58   1124.57   0.01 0  21  0.45 1  SVLVADYSSGK

 
      Proteins matching the same set of peptides:
      14360.2                           Mass: 46818    Score: 21     Peptides matched: 1
 |Crh11| CONTIG=Contig10158 POSCDS1=92919 POSCDS2=94280
      24611.1                           Mass: 47890    Score: 21     Peptides matched: 1
 CONTIG=Contig20158 POSCDS1=86016 POSCDS2=87377
8.    20153.1                           Score: 19     Peptides matched: 1
 CONTIG=Contig10119 POSCDS1=223321 POSCDS2=224067
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   410.75   819.49   819.40   0.10 0  19  1.4 2  DASTGELK

 
      Proteins matching the same set of peptides:
      25802.1                           Score: 19     Peptides matched: 1
9.    928.1                             Score: 16     Peptides matched: 1
 CONTIG=Contig2518 POSCDS1=59592 POSCDS2=62003
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   410.75   819.49   819.49   0.01 0  16  2.7 3  VSFVLQK

10.   1491.1                            Score: 14     Peptides matched: 1
 CONTIG=Contig10046 POSCDS1=232986 POSCDS2=233642
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   410.75   819.49   819.44   0.05 1  14  3.5 5  WSRMIK

 
      Proteins matching the same set of peptides:
      27039.1                           Score: 14     Peptides matched: 1
11.   20025.1                           Score: 14     Peptides matched: 1
 CONTIG=Contig10163 POSCDS1=255513 POSCDS2=259052
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   402.24   802.46   801.46   1.01 0  14  4.5 2  QLVVSEK

 
      Proteins matching the same set of peptides:
      24395.1                           Score: 14     Peptides matched: 1
12.   10638.2                           Score: 14     Peptides matched: 1
 CONTIG=Contig20215 POSCDS1=35952 POSCDS2=36452
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   410.75   819.49   819.46   0.03 1  14  3.7 6  VMKSSIR

 
      Proteins matching the same set of peptides:
      28263.1                           Score: 14     Peptides matched: 1
13.   660.1                             Score: 14     Peptides matched: 1
 CONTIG=Contig2518 POSCDS1=120781 POSCDS2=121107
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   410.75   819.49   819.39   0.10 0  14  3.8 7  VVCWEK

14.   18513.1                           Score: 13     Peptides matched: 1
 CONTIG=Contig10065 POSCDS1=21101 POSCDS2=21268
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   410.75   819.49   820.49   -0.99 0  13  4.9 8  VFIAMIK

 
      Proteins matching the same set of peptides:
      26597.1                           Score: 13     Peptides matched: 1
15.   sw|O93532|K2CO_CHICK              Score: 13     Peptides matched: 1
 Keratin, type II cytoskeletal cochleal (Cytokeratin otokeratin)
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   410.75   819.49   819.44   0.05 0  15  3.2 4  VSSVSSVR

16.   11729.2                           Score: 13     Peptides matched: 1
 CONTIG=Contig10177 POSCDS1=45463 POSCDS2=46764
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   402.24   802.46   802.45   0.01 1  13  6.7 3  LENTKAK

 
      Proteins matching the same set of peptides:
      23792.1                           Score: 13     Peptides matched: 1
17.   8358.1                            Score: 12     Peptides matched: 1
 CONTIG=Contig10046 POSCDS1=167504 POSCDS2=167905
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   410.75   819.49   818.45   1.04 1  12  5.6 9  GKSSEIAK

 
      Proteins matching the same set of peptides:
      27053.1                           Score: 12     Peptides matched: 1
18.   2175.1                            Mass: 37271    Score: 12     Peptides matched: 1
 CONTIG=Contig2506 POSCDS1=68234 POSCDS2=69229
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 2   410.75   819.49   819.40   0.10 0  12  5.7 10  SASEGEIK

19.   20170.1                           Score: 11     Peptides matched: 1
 CONTIG=Contig10053 POSCDS1=71020 POSCDS2=71511
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   495.28   988.55   989.48   -0.93 0  11  6.5 3  NSIASAGENK

 
      Proteins matching the same set of peptides:
      26819.1                           Score: 11     Peptides matched: 1
20.   9417.2                            Score: 11     Peptides matched: 1
 CONTIG=Contig10215 POSCDS1=124668 POSCDS2=126953
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 5   504.78   1007.55   1006.62   0.93 1  11  7.4 2  VILPKYFK

 
      Proteins matching the same set of peptides:
      19046.2                           Score: 11     Peptides matched: 1

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Data File Name         : cw0078_q3.pkl
Number of queries      : 10

Mascot:  http://www.matrixscience.com/ 

Top scoring peptide matches to query 1
Score greater than 29 indicates homology
Score greater than 33 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 28.2    0.01   2                         mIPF19516  LEVVTSR
 14.3    1.01  11                           20025.1  QLVVSEK
 12.6    0.01  16                           11729.2  LENTKAK
  7.1   -0.00                                        IKAAGTSR
  6.7    0.01                                        IKDVNSK
  5.0    0.05                                        IDLNNSK
  4.8    0.05                                        LEEEKR
  4.8   -0.91                                        NEEKER
  4.0    1.04                                        QLVEADK
  3.7    0.03                                        LKNWDK
Top scoring peptide matches to query 2
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 28.5    0.01   6                          mEcm33.3  VSGGFILK
 18.6    0.10   8                           20153.1  DASTGELK
 15.5    0.01   9                             928.1  VSFVLQK
 14.8    0.05  15              sw|O93532|K2CO_CHICK  VSSVSSVR
 14.4    0.05  10                            1491.1  WSRMIK
 14.2    0.03  12                           10638.2  VMKSSIR
 14.1    0.10  13                             660.1  VVCWEK
 13.0   -0.99  14                           18513.1  VFIAMIK
 12.4    1.04  17                            8358.1  GKSSEIAK
 12.4    0.10  18                            2175.1  SASEGEIK
Top scoring peptide matches to query 3
Score greater than 16 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 11.6    0.01   2                         mIPF19516  STAYLYAR
  2.8   -1.08                                        ATSLGQKLK
  0.2   -1.03                                        DLIAEKEK
Top scoring peptide matches to query 4
Score greater than 28 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 53.2    0.01   4                             mCHT2  LSSAIEEIK
 11.9   -1.00                                        NSSLSNLKK
 11.4   -0.93  19                           20170.1  NSIASAGENK
 10.4   -0.04                                        ISKLSNISK
  4.9    0.03                                        IRSEDEIK
  4.4   -0.00                                        SLNSQSLIK
  4.2   -0.04                                        SLSIITSIR
  4.2    0.06                                        QTEITAGNR
  4.1   -0.93                                        DGSNKEVNK
  3.5    1.02                                        NENIVGKSK
Top scoring peptide matches to query 5
Score greater than 25 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 39.5    0.01   2                         mIPF19516  AAALPQYFK
 10.5    0.93  20                            9417.2  VILPKYFK
  3.3   -1.04                                        LTLIHGDLK
  0.3   -0.99                                        IKGGTGWYK
  0.3    0.91                                        VLLLIDPPK
Top scoring peptide matches to query 6
Score greater than 24 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 39.1    0.00   3                        mALS3.5eoc  ISINVDFER
  8.6   -0.07                                        LSILSGFISR
  8.6   -0.03                                        ISLETLFNR
  8.2   -0.06                                        ISIGSIDLFK
  7.1    0.06                                        IAENSKCDR
  6.8   -1.00                                        TGRNVYDIR
Top scoring peptide matches to query 7
Score greater than 23 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 21.4    0.01   7                            mCrh11  SVLVADYSSGK
  8.7   -0.21                                        VSLLKVLLLK
  1.7    0.97                                        LAINGESHKR
Top scoring peptide matches to query 8
Score greater than 20 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 45.3   -0.00   5                             mRBT5  IYDQLPECAK
  4.5   -0.15                                        HRLLTEIINK
  2.7   -0.06                                        IDYQSIQIEK
  1.4   -1.02                                        DYPKIEVDMK
  0.9   -1.08                                        LKDTLEEVYK
  0.6    0.00                                        SIHHELEEDK
  0.1    1.02                                        NGTVTMLDDNR
Top scoring peptide matches to query 9
Score greater than 18 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 85.0   -0.00   1                             mSSR1  APPACLLACVAK
Top scoring peptide matches to query 10
Score greater than 22 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 14.4    0.97   3                        mALS3.5eoc  TCSSNGIFITYK
  8.7   -0.94                                        SSSSSSSSSSSSNSK
  5.4   -0.13                                        TRALNVVVNYDK
  2.1   -0.08                                        LTIGSLSEAGNSSR
  1.4   -0.10                                        TCMEAIQKVALK
  1.0    0.91                                        VPPTKPSATYSDK