Mascot Search Results

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MS data file    : cw0078_q1.pkl
Database        : CAlbicans dos (20286 sequences; 6720281 residues)
Timestamp       : 21 Mar 2005 at 15:58:39 GMT
Significant hits: mSSR1       |Candida albicans 212 aa - Secretory Stress Response
                  mALS3.5eoc  |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end
                  mPga36.1    |predicted1| mIPF10662| C. albicans fungus|CA3827| 392 aa
                  mALS1.5eoc  |predicted| C. albicans |CA0909| 658 aa - agglutinin-like,5-prime end
                  mCHT2       583 aa - chitinase 2 precursor Candida albicans
                  mRBT5       |predicted| C. albicans fungus|CA2558| 241 aa
                  19516.2     |Hyr1| CONTIG=Contig10216 POSCDS1=152086 POSCDS2=154845

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 31 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

Switch to Protein Summary Report

To create a bookmark for this report, right click this link: Peptide Summary Report (../data/20050321/F002741.dat)

        Error tolerant   

1.    mSSR1                             Mass: 20690    Score: 206    Peptides matched: 3
 |Candida albicans 212 aa - Secretory Stress Response
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
9   635.87   1269.73   1269.66   0.07 0  85  2.1e-007 1  APPACLLACVAK
12   814.91   1627.82   1627.70   0.11 0  103  2.6e-009 1  CSGLNDLSCICTTK
14   855.45   1708.88   1707.78   1.10 0  18  0.76 1  EICPNGDADTAISAFK

2.    mALS3.5eoc                        Mass: 94266    Score: 148    Peptides matched: 3
 |predicted| C. albicans |CA0591| 886 aa agglutinin-like,5-prime end
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
5   546.82   1091.63   1091.56   0.07 0  38  0.012 1  ISINVDFER
10   695.89   1389.76   1389.66   0.10 0  37  0.014 1  TCSSNGIFITYK
13   845.45   1688.88   1688.75   0.13 0  73  2.7e-006 1  CFTAGTNTVTFNDGGK

 
      Proteins matching the same set of peptides:
      12561.3                           Mass: 95687    Score: 148    Peptides matched: 3
 |Als3.5'| CONTIG=Contig10129 POSCDS1=15287 POSCDS2=17932
      25511.1                           Mass: 95687    Score: 148    Peptides matched: 3
 |Als3.5'| CONTIG=Contig20129 POSCDS1=15283 POSCDS2=17928
3.    mPga36.1                          Mass: 35596    Score: 121    Peptides matched: 1
 |predicted1| mIPF10662| C. albicans fungus|CA3827| 392 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
15   934.97   1867.92   1867.82   0.10 0  121  3.2e-011 1  QQSCGGTDDVSSQSATLK

 
      Proteins matching the same set of peptides:
      mPga36.2                          Mass: 35170    Score: 121    Peptides matched: 1
 |predicted2| mIPF10662| C. albicans fungus|CA3827| 392 aa
      10662.2                           Mass: 37379    Score: 121    Peptides matched: 1
 |Pga36| CONTIG=Contig10233 POSCDS1=119212 POSCDS2=120390
      21466.1                           Mass: 39845    Score: 121    Peptides matched: 1
 CONTIG=Contig20233 POSCDS1=118488 POSCDS2=119666
4.    mALS1.5eoc                        Mass: 69052    Score: 56     Peptides matched: 1
 |predicted| C. albicans |CA0909| 658 aa - agglutinin-like,5-prime end
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
11   708.40   1414.78   1414.67   0.11 0  56  0.00017 1  STVDPSGYLYASR

 
      Proteins matching the same set of peptides:
      13002.2                           Mass: 133694   Score: 56     Peptides matched: 1
 |Als1| CONTIG=Contig10233 POSCDS1=72223 POSCDS2=76005
      21498.1                           Mass: 133402   Score: 56     Peptides matched: 1
 |Als1| CONTIG=Contig20233 POSCDS1=71475 POSCDS2=75257
5.    mCHT2                             Mass: 59565    Score: 49     Peptides matched: 1
 583 aa - chitinase 2 precursor Candida albicans
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
3   495.31   988.61   988.54   0.07 0  49  0.0012 1  LSSAIEEIK

 
      Proteins matching the same set of peptides:
      15860.2                           Mass: 61554    Score: 49     Peptides matched: 1
 |Cht2| CONTIG=Contig10194 POSCDS1=2376 POSCDS2=4127
      19719.2                           Mass: 51350    Score: 49     Peptides matched: 1
 CONTIG=Contig20194 POSCDS1=2710 POSCDS2=4131
6.    mRBT5                             Mass: 22745    Score: 45     Peptides matched: 1
 |predicted| C. albicans fungus|CA2558| 241 aa
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
8   618.83   1235.65   1235.59   0.07 0  45  0.002 1  IYDQLPECAK

 
      Proteins matching the same set of peptides:
      mPga10                            Mass: 23610    Score: 45     Peptides matched: 1
 |predicted| C. albicans fungus|CA4381|mIPF20169: 250 aa
      19952.1                           Mass: 24614    Score: 45     Peptides matched: 1
 |Rbt5| CONTIG=Contig10231 POSCDS1=6478 POSCDS2=7203
      20169.1                           Mass: 25603    Score: 45     Peptides matched: 1
 |Pga10| CONTIG=Contig10231 POSCDS1=59560 POSCDS2=60312
      21543.1                           Mass: 25731    Score: 45     Peptides matched: 1
 CONTIG=Contig20231 POSCDS1=59564 POSCDS2=60316
      21544.1                           Mass: 25457    Score: 45     Peptides matched: 1
 CONTIG=Contig20231 POSCDS1=6478 POSCDS2=7203
7.    19516.2                           Mass: 94340    Score: 39     Peptides matched: 1
 |Hyr1| CONTIG=Contig10216 POSCDS1=152086 POSCDS2=154845
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
4   504.83   1007.64   1007.54   0.09 0  39  0.011 1  AAALPQYFK

 
      Proteins matching the same set of peptides:
      mIPF19516                         Mass: 90303    Score: 39     Peptides matched: 1
 |mHYR1| validated by us| C. albicans fungus|CA1576| 900 aa
      19734.1                           Mass: 94169    Score: 39     Peptides matched: 1
 |Hyr1| CONTIG=Contig20216 POSCDS1=152490 POSCDS2=155303
      m19734.1                          Mass: 91992    Score: 39     Peptides matched: 1
 |mHyr1| validated by us| C. albicans fungus 917 aa
8.    mEcm33.3                          Mass: 41984    Score: 31     Peptides matched: 1
 |predicted|m5366.2|CaECM33
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   410.77   819.53   819.49   0.05 0  31  0.082 1  VSGGFILK

 
      Proteins matching the same set of peptides:
      5366.2                            Mass: 43798    Score: 31     Peptides matched: 1
 |Ecm33.3| CaECM33 CONTIG=Contig10163 POSCDS1=164904 POSCDS2=166142
      24311.1                           Mass: 42880    Score: 31     Peptides matched: 1
 CONTIG=Contig20163 POSCDS1=164513 POSCDS2=165751
9.    mCrh11                            Mass: 44667    Score: 28     Peptides matched: 1
 |predicted| C. albicans |CA0375|: 453 aa
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
7   563.33   1124.65   1124.57   0.08 0  28  0.094 1  SVLVADYSSGK

 
      Proteins matching the same set of peptides:
      14360.2                           Mass: 46818    Score: 28     Peptides matched: 1
 |Crh11| CONTIG=Contig10158 POSCDS1=92919 POSCDS2=94280
      24611.1                           Mass: 47890    Score: 28     Peptides matched: 1
 CONTIG=Contig20158 POSCDS1=86016 POSCDS2=87377
10.   mIPF8527|mPga29:                  Mass: 19928    Score: 25     Peptides matched: 1
 189 aa - Candida albicans unknown function
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
6   554.36   1106.70   1106.63   0.07 0  25  0.22 1  TISSIQLFAK

11.   18513.1                           Score: 22     Peptides matched: 1
 CONTIG=Contig10065 POSCDS1=21101 POSCDS2=21268
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   410.77   819.53   820.49   -0.96 0  22  0.65 2  VFIAMIK

 
      Proteins matching the same set of peptides:
      26597.1                           Score: 22     Peptides matched: 1
12.   20170.1                           Mass: 17760    Score: 17     Peptides matched: 1
 CONTIG=Contig10053 POSCDS1=71020 POSCDS2=71511
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.31   988.61   989.48   -0.86 0  17  1.7 3  NSIASAGENK

 
      Proteins matching the same set of peptides:
      26819.1                           Mass: 17760    Score: 17     Peptides matched: 1
 CONTIG=Contig20053 POSCDS1=70977 POSCDS2=71468
13.   8495.2                            Score: 17     Peptides matched: 1
 CONTIG=Contig10163 POSCDS1=196876 POSCDS2=198465
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.31   988.61   989.55   -0.94 1  19  1.1 2  NSSLSNLKK

 
      Proteins matching the same set of peptides:
      24293.1                           Score: 17     Peptides matched: 1
14.   10019.2                           Score: 17     Peptides matched: 1
 CONTIG=Contig10194 POSCDS1=31204 POSCDS2=31500
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   410.77   819.53   819.32   0.21 0  17  1.9 3  DADADADK

 
      Proteins matching the same set of peptides:
      23328.1                           Score: 17     Peptides matched: 1
15.   23687.1                           Score: 17     Peptides matched: 1
 CONTIG=Contig20184 POSCDS1=49345 POSCDS2=49773
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 7   563.33   1124.65   1124.79   -0.14 1  17  1.4 2  VSLLKVLLLK

 
      Proteins matching the same set of peptides:
      28713.1                           Score: 17     Peptides matched: 1
16.   928.1                             Score: 13     Peptides matched: 1
 CONTIG=Contig2518 POSCDS1=59592 POSCDS2=62003
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   410.77   819.53   819.49   0.05 0  13  5.2 4  VSFVLQK

17.   10160.1                           Score: 13     Peptides matched: 1
 CONTIG=Contig10212 POSCDS1=115588 POSCDS2=115980
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.31   988.61   987.53   1.08 1  13  4.9 4  NENIVGKSK

 
      Proteins matching the same set of peptides:
      22646.1                           Score: 13     Peptides matched: 1
18.   7525.1                            Score: 13     Peptides matched: 1
 CONTIG=Contig10209 POSCDS1=14882 POSCDS2=15388
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 3   495.31   988.61   988.53   0.08 0  13  5 5  ISTHAVSFK

 
      Proteins matching the same set of peptides:
      22725.1                           Score: 13     Peptides matched: 1
19.   9417.2                            Score: 12     Peptides matched: 1
 CONTIG=Contig10215 POSCDS1=124668 POSCDS2=126953
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 4   504.83   1007.64   1006.62   1.01 1  12  5.4 2  VILPKYFK

 
      Proteins matching the same set of peptides:
      19046.2                           Score: 12     Peptides matched: 1
20.   2404.1                            Score: 11     Peptides matched: 1
 CONTIG=Contig10254 POSCDS1=197616 POSCDS2=199169
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   410.77   819.53   820.51   -0.97 0  11  7.1 5  VVSYLIK

 
      Proteins matching the same set of peptides:
      20357.1                           Score: 11     Peptides matched: 1
Peptide matches not assigned to protein hits: (no details means no match)
List sorted by Decreasing Score. Sort by Decreasing Intensity or Increasing Query # / Mr

      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
2   486.77   971.53   972.54   -1.01 1  5  34 1  NVAKSVAER

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 1.2 Da
Fragment Mass Tolerance: ± 0.6 Da
Max Missed Cleavages   : 1
Instrument type        : ESI-QUAD-TOF
Data File Name         : cw0078_q1.pkl
Number of queries      : 15

Mascot:  http://www.matrixscience.com/ 

Top scoring peptide matches to query 1
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 30.8    0.05   8                          mEcm33.3  VSGGFILK
 21.8   -0.96  11                           18513.1  VFIAMIK
 17.1    0.21  14                           10019.2  DADADADK
 12.8    0.05  16                             928.1  VSFVLQK
 11.4   -0.97  20                            2404.1  VVSYLIK
 10.5    0.09                                        VSSVSSVR
  9.9   -0.88                                        VSSCAGIK
  8.0   -0.97                                        YITALLK
  7.4    0.12                                        VCSDVIK
  7.2    0.16                                        VSNESER
Top scoring peptide matches to query 2
Score greater than 13 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
  4.5   -1.01                                        NVAKSVAER
  0.2   -0.98                                        HTPYNITK
Top scoring peptide matches to query 3
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 48.7    0.07   5                             mCHT2  LSSAIEEIK
 19.2   -0.94  13                            8495.2  NSSLSNLKK
 17.3   -0.86  12                           20170.1  NSIASAGENK
 12.7    1.08  17                           10160.1  NENIVGKSK
 12.6    0.08  18                            7525.1  ISTHAVSFK
 10.5    0.02                                        SLSIITSIR
  9.1    0.02                                        ISKLSNISK
  8.6   -0.90                                        SNSISQLNK
  8.6    0.02                                        SIISTISLR
  7.4    0.09                                        ISKEDEIR
Top scoring peptide matches to query 4
Score greater than 27 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 38.7    0.09   7                           19516.2  AAALPQYFK
 11.8    1.01  19                            9417.2  VILPKYFK
  0.7   -0.90                                        IKGGTGWYK
  0.1   -0.96                                        LTLIHGDLK
  0.1    0.99                                        VLLLIDPPK
Top scoring peptide matches to query 5
Score greater than 23 indicates homology
Score greater than 32 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 38.4    0.07   2                        mALS3.5eoc  ISINVDFER
  8.0   -0.00                                        LSILSGFISR
  7.7    0.03                                        ISLETLFNR
  7.4    0.01                                        ISIGSIDLFK
  6.7    0.13                                        IAENSKCDR
  6.3   -0.94                                        TGRNVYDIR
  3.1   -0.86                                        NSGSESNSRR
  1.8   -0.99                                        LTVLTNSAFK
Top scoring peptide matches to query 6
Score greater than 21 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 25.2    0.07  10                  mIPF8527|mPga29:  TISSIQLFAK
  6.8    0.13                                        TSNAKLMQSK
  6.6    0.14                                        FILAGEDNTK
  4.4    0.07                                        YSELNKILK
  2.8    1.17                                        AEKTASESQR
  1.7    1.05                                        VFGGLYPILK
  1.2    0.18                                        DVSEGAGPTFK
  1.2    1.18                                        VDQAETCRK
  1.0   -0.95                                        NLHSQRIIK
Top scoring peptide matches to query 7
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 28.2    0.08   9                            mCrh11  SVLVADYSSGK
 16.6   -0.14  15                           23687.1  VSLLKVLLLK
  5.6    0.05                                        RQNQVQQPK
  3.4    1.04                                        GPSGVKSHISR
  2.7    0.06                                        WIFIGSSSTK
  2.7    0.09                                        WLIEENGHK
  2.7    0.10                                        SVLLYDEMR
  1.0   -0.02                                        INWIPKGAVK
  0.2   -0.93                                        GPNNQELINK
  0.1   -1.06                                        LLRHPHIIK
Top scoring peptide matches to query 8
Score greater than 24 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 45.3    0.07   6                             mRBT5  IYDQLPECAK
  9.0   -0.95                                        DYPKIEVDMK
  5.0    0.92                                        YLTLKIVETR
  3.4    0.07                                        SIHHELEEDK
  3.0    0.01                                        IDYQSIQIEK
  0.7   -0.08                                        HRLLTEIINK
  0.3   -0.90                                        KDTMELEEDK
Top scoring peptide matches to query 9
Score greater than 18 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 84.8    0.07   1                             mSSR1  APPACLLACVAK
Top scoring peptide matches to query 10
Score greater than 17 indicates homology
Score greater than 31 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 36.8    0.10   2                        mALS3.5eoc  TCSSNGIFITYK
  2.1   -0.99                                        FASISLSGGVQPTK
  1.6    0.04                                        VPPTKPSATYSDK
  0.7    1.04                                        HESITSVITSSTK
Top scoring peptide matches to query 11
Score greater than 23 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 55.6    0.11   4                        mALS1.5eoc  STVDPSGYLYASR
  6.9    0.14                                        STTMQDSFDQKK
  2.0   -0.02                                        IAMLLFNIPDIR
  1.9    0.00                                        LRTDLLETADIR
  1.1    1.11                                        STVDQSGYLTTSR
Top scoring peptide matches to query 12
Score greater than 21 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
102.8    0.11   1                             mSSR1  CSGLNDLSCICTTK
  1.9   -0.98                                        DFVFIERNNNSFK
  0.7    0.01                                        YTNDEQYVLIATAK
  0.2   -1.05                                        FSKMVELVSFAPFK
Top scoring peptide matches to query 13
Score greater than 17 indicates homology
Score greater than 30 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 73.0    0.13   2                        mALS3.5eoc  CFTAGTNTVTFNDGGK
Top scoring peptide matches to query 14
Score greater than 14 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
 18.1    1.10   1                             mSSR1  EICPNGDADTAISAFK
Top scoring peptide matches to query 15
Score greater than 23 indicates homology
Score greater than 29 indicates identity
Status bar shows all hits for this peptide
 
Score   Delta  Hit                         Protein   Peptide
121.5    0.10   3                          mPga36.1  QQSCGGTDDVSSQSATLK
  2.5    0.99                                        SIGDWNFVKTIGAGSMGK
  2.1    0.90                                        NDIHKILLGESQYIPK
  1.2    0.02                                        FIENILSSGSDMEIWK
  1.2   -0.11                                        SLMIDSSKLLGLYSTLK
  0.3   -0.12                                        LINPDNIQIIVITMGSK
  0.1    1.03                                        MWNEFEWENKITIK